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The map label for this gene is yisY [H]

Identifier: 197116741

GI number: 197116741

Start: 417206

End: 418003

Strand: Reverse

Name: yisY [H]

Synonym: Gbem_0341

Alternate gene names: 197116741

Gene position: 418003-417206 (Counterclockwise)

Preceding gene: 197116742

Following gene: 197116740

Centisome position: 9.06

GC content: 63.28

Gene sequence:

>798_bases
ATGCTAACGCAAATAAACAACATCCGCCTCGCCTACGACGACCTCGGCCACGGCCCGGCCGTCTTGCTCATCCACGGCTT
CCCGCTGAACCGCCAGATGTGGCAGCCGCAATTAAAACCGTTGGCCGACGCCGGCTATCGCGCCATCGCGCCGGACCTGC
GCGGCTTCGGCGCCAGCGACGCGCCCGCTGCCGGCTACAGCATGGACCGCTTCGCCGACGACCTGGTGGCGCTGCTCGAT
GCGCTGCAGATCGACAAGGCAGTGGTGGGCGGGATGTCCATGGGGGGATACATCCTCATGAACCTCCTGGAGCGCCGCCC
CGACCGGGTCCGCGCCGCAGCCTTCATCGCCACCAGGAGCAACGCCGACGACGAAGCTGCACGCCAGCGCAGAAGTGAAA
TGGCCGCCCAAGCCGAAAGGCTGGGGGCAAACCCGATTACGAAGATCTTCGCCGAACTTCTTTTTGCCGACGACACCACC
GTGTCCCGCCCGGAACTTATCGCTCAGGTCACCTCCTGGATGCGCTCCACCAACCCCAATGGACTCGCCGGTGGCCTGGT
CGCCATCAGGGACAGGAAGGAATACACTCCCCTTCTCGCCTCCTTCGGCAAGCCTTCGCTCGTTATCGCCGGAACCGAGG
ATCGGGCCGCGCCGCTTGATGTCGCGAGGGTGCTAATCGAGGCGCTCCCCGGCTGCCGGACCAAAATCATCGAAAAGGCG
GGGCACATGGTTAACATGGAACAACCAGAACTCTTCAACAAGACGCTGGTCGACTTCCTCGATTCGCTTCCTGATTAG

Upstream 100 bases:

>100_bases
ACTACTGACCTCCATAATTAAAACGACGAAGATGCACAACAAAAAATAACCATTGTCAATTGTCAATTGTCAATCGTCAA
TTGGTTCAAAGGGTTTCTTC

Downstream 100 bases:

>100_bases
CGGGATTAAACATGACACCTGACCTCTTTCAATTGATCATCCTGCTGCTTGGCGCCGCCACCTTCGTGTTCGCGTTCCTT
TGCTACCTGCATCTCAAACG

Product: hydrolase or acyltransferase, alpha/beta fold family

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLTQINNIRLAYDDLGHGPAVLLIHGFPLNRQMWQPQLKPLADAGYRAIAPDLRGFGASDAPAAGYSMDRFADDLVALLD
ALQIDKAVVGGMSMGGYILMNLLERRPDRVRAAAFIATRSNADDEAARQRRSEMAAQAERLGANPITKIFAELLFADDTT
VSRPELIAQVTSWMRSTNPNGLAGGLVAIRDRKEYTPLLASFGKPSLVIAGTEDRAAPLDVARVLIEALPGCRTKIIEKA
GHMVNMEQPELFNKTLVDFLDSLPD

Sequences:

>Translated_265_residues
MLTQINNIRLAYDDLGHGPAVLLIHGFPLNRQMWQPQLKPLADAGYRAIAPDLRGFGASDAPAAGYSMDRFADDLVALLD
ALQIDKAVVGGMSMGGYILMNLLERRPDRVRAAAFIATRSNADDEAARQRRSEMAAQAERLGANPITKIFAELLFADDTT
VSRPELIAQVTSWMRSTNPNGLAGGLVAIRDRKEYTPLLASFGKPSLVIAGTEDRAAPLDVARVLIEALPGCRTKIIEKA
GHMVNMEQPELFNKTLVDFLDSLPD
>Mature_265_residues
MLTQINNIRLAYDDLGHGPAVLLIHGFPLNRQMWQPQLKPLADAGYRAIAPDLRGFGASDAPAAGYSMDRFADDLVALLD
ALQIDKAVVGGMSMGGYILMNLLERRPDRVRAAAFIATRSNADDEAARQRRSEMAAQAERLGANPITKIFAELLFADDTT
VSRPELIAQVTSWMRSTNPNGLAGGLVAIRDRKEYTPLLASFGKPSLVIAGTEDRAAPLDVARVLIEALPGCRTKIIEKA
GHMVNMEQPELFNKTLVDFLDSLPD

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=297, Percent_Identity=27.6094276094276, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554012, Length=290, Percent_Identity=26.551724137931, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 28869; Mature: 28869

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQINNIRLAYDDLGHGPAVLLIHGFPLNRQMWQPQLKPLADAGYRAIAPDLRGFGASD
CCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHCCCHHHHHHHCCCHHHCCHHHCCCCCC
APAAGYSMDRFADDLVALLDALQIDKAVVGGMSMGGYILMNLLERRPDRVRAAAFIATRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
NADDEAARQRRSEMAAQAERLGANPITKIFAELLFADDTTVSRPELIAQVTSWMRSTNPN
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
GLAGGLVAIRDRKEYTPLLASFGKPSLVIAGTEDRAAPLDVARVLIEALPGCRTKIIEKA
CCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
GHMVNMEQPELFNKTLVDFLDSLPD
CCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTQINNIRLAYDDLGHGPAVLLIHGFPLNRQMWQPQLKPLADAGYRAIAPDLRGFGASD
CCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHCCCHHHHHHHCCCHHHCCHHHCCCCCC
APAAGYSMDRFADDLVALLDALQIDKAVVGGMSMGGYILMNLLERRPDRVRAAAFIATRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
NADDEAARQRRSEMAAQAERLGANPITKIFAELLFADDTTVSRPELIAQVTSWMRSTNPN
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
GLAGGLVAIRDRKEYTPLLASFGKPSLVIAGTEDRAAPLDVARVLIEALPGCRTKIIEKA
CCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
GHMVNMEQPELFNKTLVDFLDSLPD
CCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]