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The map label for this gene is fnpA [H]

Identifier: 197116421

GI number: 197116421

Start: 27683

End: 29092

Strand: Reverse

Name: fnpA [H]

Synonym: Gbem_0021

Alternate gene names: 197116421

Gene position: 29092-27683 (Counterclockwise)

Preceding gene: 197116422

Following gene: 197116420

Centisome position: 0.63

GC content: 66.17

Gene sequence:

>1410_bases
GTGAGCAACGATTTGTCCCCCAAAGAGAGGATGGCTATAGAGAGGGTCCACATGCCGGAGCTTCCGGCGGACGAGAGAAG
CAAGAATTTCGAAGAGGTGAACCAAGGGCTCAGTCCTGATCAGGCGGTCGCCGAGGCGCAGCGCTGCCTGCAGTGCAAAA
GCAGGAACTGTGTGGCCGGGTGCCCGGTCGGCGTCTCCATTCCCGAATTCATCGACGCGCTGGCAAGCGACGACATGCCC
AGGGCGGCCCAGGTACTGCGCGGCGACAACGCGCTTCCTGCGGTCTGCGGCAGGGTCTGTCCCCAGGAAACGCAGTGCGA
GGCGCTCTGCGTGCGCGGCAAGAAGGGGGACGCGGTGGCAATCGGCTACCTGGAGCGCTACGTGGCCGACTGGGCCATGC
AACATCCCGACGAGCTGATAACGGAAGATGCGCCGAAGCCGACCGGCAAATCGGTGGCCGTGGTCGGCTGCGGCCCGGCA
GGACTCACCGCCGCCGGTGAGTTGGCCCGGCAGGGGCACAAGGTGACCATCTTCGAAGCGCTGCACGACACCGGCGGGGT
GCTCCGCTACGGCATCCCCGAGTTCCGGCTTCCCAAGGAAATCATCGATCGCGAGGTGGCGGTGCTGAGCCGGATGGGGG
TTGCCATCGAGTGCAACGTCATCATCGGCAAGACCCTCACCATTCCGCAGCTGCGCAGCGAGTTCGACGCCGTCTTCATC
GCCAACGGCGCGGGCCTTCCCACCATGCTGAACATCATGGGCGAGAACCTGAAGGGGGTCTACGCCGCCAATGAGTTCCT
CACCCGCGTGAACCTCATGGAAGCCGGGCGCAGGGCCGACAGCTCCACCCCGATCCTGCAGCGGGACGAGGTAGCGGTGA
TCGGCGGCGGCAACACCGCCATGGACTGCGTGCGTACCGCCCGCAGGTTGGGCGCCAAGCGCGCCATGATCGTCTACCGC
CGCAGCGAGACCGAGATGCCTGCCAGGGTCGAAGAGATCAAGCACGCCAAGGAGGAAGGTGTTGAGTTCATCATGCTCAC
CGCCCCCGTCGCCATCGTCGCCACCGAGGACGGCTGGGTCTCCGCCTTGCGCTGCCAGAAGATGGAGCTCGGCCCCGCCG
ACGACTCAGGCCGCCGCAGGCCCCAGGCCATAGAAGGCTCCGAGTTCGACCTTCCCGCCGGAATCGTCATCAACGCCGTC
GGCACCAGCGCGAACCCGCTCCTCACCGCGACGGCTCCCGACCTCACCCTCAACAAGTGGGGCAACATCGTCACCAACGA
CGAAGGGGAGACCAGCATCCCCGGCGTCTTCGCCGGCGGCGACATCGTCAGGGGCGGGGCCACGGTCATCCTGGCCATGG
GCGACGGCAAGCGCGCCGCAGCCGCTATCGACAAGTACCTGAAAGGCTAG

Upstream 100 bases:

>100_bases
TCGATTTCGACGGGCTTTCCGACCGCCTCACCAGCTATCGCAAGGAAGAGGCGCTAAGGCATGCAGCGACGGATTGCAAG
ATAGCTAAGGAGGTAGCAAA

Downstream 100 bases:

>100_bases
CCAGAGCTAGCAGGAACAGCCTAGGGGGCGCCGCAAGGCGCCCCCTCTTTTCCACCCGCCCCCTCGCCTCCATCATCCCA
TTCCTATTGCCGTTCCGGTT

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSNDLSPKERMAIERVHMPELPADERSKNFEEVNQGLSPDQAVAEAQRCLQCKSRNCVAGCPVGVSIPEFIDALASDDMP
RAAQVLRGDNALPAVCGRVCPQETQCEALCVRGKKGDAVAIGYLERYVADWAMQHPDELITEDAPKPTGKSVAVVGCGPA
GLTAAGELARQGHKVTIFEALHDTGGVLRYGIPEFRLPKEIIDREVAVLSRMGVAIECNVIIGKTLTIPQLRSEFDAVFI
ANGAGLPTMLNIMGENLKGVYAANEFLTRVNLMEAGRRADSSTPILQRDEVAVIGGGNTAMDCVRTARRLGAKRAMIVYR
RSETEMPARVEEIKHAKEEGVEFIMLTAPVAIVATEDGWVSALRCQKMELGPADDSGRRRPQAIEGSEFDLPAGIVINAV
GTSANPLLTATAPDLTLNKWGNIVTNDEGETSIPGVFAGGDIVRGGATVILAMGDGKRAAAAIDKYLKG

Sequences:

>Translated_469_residues
MSNDLSPKERMAIERVHMPELPADERSKNFEEVNQGLSPDQAVAEAQRCLQCKSRNCVAGCPVGVSIPEFIDALASDDMP
RAAQVLRGDNALPAVCGRVCPQETQCEALCVRGKKGDAVAIGYLERYVADWAMQHPDELITEDAPKPTGKSVAVVGCGPA
GLTAAGELARQGHKVTIFEALHDTGGVLRYGIPEFRLPKEIIDREVAVLSRMGVAIECNVIIGKTLTIPQLRSEFDAVFI
ANGAGLPTMLNIMGENLKGVYAANEFLTRVNLMEAGRRADSSTPILQRDEVAVIGGGNTAMDCVRTARRLGAKRAMIVYR
RSETEMPARVEEIKHAKEEGVEFIMLTAPVAIVATEDGWVSALRCQKMELGPADDSGRRRPQAIEGSEFDLPAGIVINAV
GTSANPLLTATAPDLTLNKWGNIVTNDEGETSIPGVFAGGDIVRGGATVILAMGDGKRAAAAIDKYLKG
>Mature_468_residues
SNDLSPKERMAIERVHMPELPADERSKNFEEVNQGLSPDQAVAEAQRCLQCKSRNCVAGCPVGVSIPEFIDALASDDMPR
AAQVLRGDNALPAVCGRVCPQETQCEALCVRGKKGDAVAIGYLERYVADWAMQHPDELITEDAPKPTGKSVAVVGCGPAG
LTAAGELARQGHKVTIFEALHDTGGVLRYGIPEFRLPKEIIDREVAVLSRMGVAIECNVIIGKTLTIPQLRSEFDAVFIA
NGAGLPTMLNIMGENLKGVYAANEFLTRVNLMEAGRRADSSTPILQRDEVAVIGGGNTAMDCVRTARRLGAKRAMIVYRR
SETEMPARVEEIKHAKEEGVEFIMLTAPVAIVATEDGWVSALRCQKMELGPADDSGRRRPQAIEGSEFDLPAGIVINAVG
TSANPLLTATAPDLTLNKWGNIVTNDEGETSIPGVFAGGDIVRGGATVILAMGDGKRAAAAIDKYLKG

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=465, Percent_Identity=31.1827956989247, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=32.2784810126582, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI111118983, Length=158, Percent_Identity=32.2784810126582, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789606, Length=465, Percent_Identity=37.8494623655914, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082180, Length=473, Percent_Identity=37.2093023255814, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI1788811, Length=459, Percent_Identity=37.2549019607843, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788468, Length=448, Percent_Identity=35.0446428571429, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789243, Length=459, Percent_Identity=33.9869281045752, Blast_Score=192, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI71984108, Length=461, Percent_Identity=32.9718004338395, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17570289, Length=484, Percent_Identity=29.9586776859504, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=165, Percent_Identity=33.9393939393939, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=30.3092783505155, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320560, Length=348, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665547, Length=481, Percent_Identity=32.6403326403326, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI24665543, Length=481, Percent_Identity=32.6403326403326, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI28574881, Length=478, Percent_Identity=32.8451882845188, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24665539, Length=478, Percent_Identity=32.8451882845188, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24640763, Length=465, Percent_Identity=30.752688172043, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI18858217, Length=465, Percent_Identity=30.752688172043, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50100; Mature: 49969

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDLSPKERMAIERVHMPELPADERSKNFEEVNQGLSPDQAVAEAQRCLQCKSRNCVAG
CCCCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEE
CPVGVSIPEFIDALASDDMPRAAQVLRGDNALPAVCGRVCPQETQCEALCVRGKKGDAVA
CCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHEEECCCCCEEE
IGYLERYVADWAMQHPDELITEDAPKPTGKSVAVVGCGPAGLTAAGELARQGHKVTIFEA
HHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEE
LHDTGGVLRYGIPEFRLPKEIIDREVAVLSRMGVAIECNVIIGKTLTIPQLRSEFDAVFI
HHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHCCCCEEEE
ANGAGLPTMLNIMGENLKGVYAANEFLTRVNLMEAGRRADSSTPILQRDEVAVIGGGNTA
ECCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEECCCHH
MDCVRTARRLGAKRAMIVYRRSETEMPARVEEIKHAKEEGVEFIMLTAPVAIVATEDGWV
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEEECCCHH
SALRCQKMELGPADDSGRRRPQAIEGSEFDLPAGIVINAVGTSANPLLTATAPDLTLNKW
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHCC
GNIVTNDEGETSIPGVFAGGDIVRGGATVILAMGDGKRAAAAIDKYLKG
CCEEECCCCCCCCCEEEECCCEEECCEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SNDLSPKERMAIERVHMPELPADERSKNFEEVNQGLSPDQAVAEAQRCLQCKSRNCVAG
CCCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEE
CPVGVSIPEFIDALASDDMPRAAQVLRGDNALPAVCGRVCPQETQCEALCVRGKKGDAVA
CCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHEEECCCCCEEE
IGYLERYVADWAMQHPDELITEDAPKPTGKSVAVVGCGPAGLTAAGELARQGHKVTIFEA
HHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEE
LHDTGGVLRYGIPEFRLPKEIIDREVAVLSRMGVAIECNVIIGKTLTIPQLRSEFDAVFI
HHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHCCCCEEEE
ANGAGLPTMLNIMGENLKGVYAANEFLTRVNLMEAGRRADSSTPILQRDEVAVIGGGNTA
ECCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEEECCCHH
MDCVRTARRLGAKRAMIVYRRSETEMPARVEEIKHAKEEGVEFIMLTAPVAIVATEDGWV
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCEEEEEECCCHH
SALRCQKMELGPADDSGRRRPQAIEGSEFDLPAGIVINAVGTSANPLLTATAPDLTLNKW
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHCC
GNIVTNDEGETSIPGVFAGGDIVRGGATVILAMGDGKRAAAAIDKYLKG
CCEEECCCCCCCCCEEEECCCEEECCEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]