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The map label for this gene is dnaN [H]

Identifier: 197116404

GI number: 197116404

Start: 1757

End: 2875

Strand: Direct

Name: dnaN [H]

Synonym: Gbem_0002

Alternate gene names: 197116404

Gene position: 1757-2875 (Clockwise)

Preceding gene: 197116403

Following gene: 197116405

Centisome position: 0.04

GC content: 53.8

Gene sequence:

>1119_bases
ATGGAGTTCAGAATCAGCAAAGAGATTTTTATGAAAGCCCTCCAGAGAATCCAGGGTATCGTAGAAAAACGCAATACCAT
GCCGATTCTCTCCAACGCGCTGCTGGAGGTTCAAAACGACCAGCTCTTCATCACGGCCACCGATCTCGAGGTAGGCATGA
AGAGCTCCTACCCCACTGCCGTCATCAGGGAAGGAAAGATCACCGTATCGGCCAAAAAGCTTTACGAGATCGTCAAGGAG
ATGCCGGACGAGGAGATTCACTTCACCACCAAGGACAACGACTGGGTAGAGATCACCTGCGGCAAGGCGCGATTCAACAT
AGTCGGGCTTTCATCCGAAGAGTTCCCTTATTTCCCCAAGGTGAAGGAAGAGAGCTTCCTGGTCTTCAAGAACGAGATTC
TCGCAGACATGATCGAGAAGACCTCCTATGCCATCTGCTATGACGAGACCAAGTACAACCTGAACGGCGTCTTCATCAAA
GCGTTCGACGAAAACGGAGAGAACGTGCTCAAGATGGTGGCGACAGACGGTCACCGACTATCCGTCTCTCAGAAAGCGAT
CACCGGCAACATCACTTCCGAGCTCTCCAAAGGGGTCATCTTCCCCAAGAAGGGTATCTTCGAGCTGAAAAAGATGGTGG
AGGAGGAAGACGGGGAGATCATGCTCGGCTTCATGGACAACTCCGCGGTCATCAAGAAAGGGAACACGGTGGTGGTCATG
CGGCTCATCGACGGCGAGTTCCCGGACTACACCAAGGTGATTCCAGCCAGCAACGACCGGATCGTGCAGATAAACCGCGA
CAAGTTCCTGCACTCGCTGAAAAGGATGGCGATCCTCTCCAGCGAGAAGTTCAAGGGGGTCAAGATAGAGGTGAACCCCG
AGCTGGTGATTATCTCGTCCAGCAACCCGGAACTCGGCGAGGCGCGCGAAGAGATCGAGGTCCAATACGAAGGCAACAAC
ATCTCGGCCCGCTTCAACGCGAAGTACCTGATCGACGTCCTGTCGGTAATGGAGCAGCGCGAAGTGGAGCTGAAGCTCAA
GGACGAACTCTCCCCGGTCATCATGAAGGCAGCGGCAGAGGAGGAGTTCCTGGCAGTCATCATGCCGATGAGGCTTTAA

Upstream 100 bases:

>100_bases
TTTTCAAAGGGTAAGAGGGCTTGTCCACTTATCAACAGGCCACTATCTACTACTACTTTAAAAACTTTAAAAACATAATA
GAAAAAGAACCGGGGGACAC

Downstream 100 bases:

>100_bases
AAACAGTTCAGAGGCAGATTGAGATTAAGATTAAGAAAACCGAAACAACAGGCGGAGCTATTGATCATTTACTGTAACAG
CCAGGTTTTCTTAATCTCAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEFRISKEIFMKALQRIQGIVEKRNTMPILSNALLEVQNDQLFITATDLEVGMKSSYPTAVIREGKITVSAKKLYEIVKE
MPDEEIHFTTKDNDWVEITCGKARFNIVGLSSEEFPYFPKVKEESFLVFKNEILADMIEKTSYAICYDETKYNLNGVFIK
AFDENGENVLKMVATDGHRLSVSQKAITGNITSELSKGVIFPKKGIFELKKMVEEEDGEIMLGFMDNSAVIKKGNTVVVM
RLIDGEFPDYTKVIPASNDRIVQINRDKFLHSLKRMAILSSEKFKGVKIEVNPELVIISSSNPELGEAREEIEVQYEGNN
ISARFNAKYLIDVLSVMEQREVELKLKDELSPVIMKAAAEEEFLAVIMPMRL

Sequences:

>Translated_372_residues
MEFRISKEIFMKALQRIQGIVEKRNTMPILSNALLEVQNDQLFITATDLEVGMKSSYPTAVIREGKITVSAKKLYEIVKE
MPDEEIHFTTKDNDWVEITCGKARFNIVGLSSEEFPYFPKVKEESFLVFKNEILADMIEKTSYAICYDETKYNLNGVFIK
AFDENGENVLKMVATDGHRLSVSQKAITGNITSELSKGVIFPKKGIFELKKMVEEEDGEIMLGFMDNSAVIKKGNTVVVM
RLIDGEFPDYTKVIPASNDRIVQINRDKFLHSLKRMAILSSEKFKGVKIEVNPELVIISSSNPELGEAREEIEVQYEGNN
ISARFNAKYLIDVLSVMEQREVELKLKDELSPVIMKAAAEEEFLAVIMPMRL
>Mature_372_residues
MEFRISKEIFMKALQRIQGIVEKRNTMPILSNALLEVQNDQLFITATDLEVGMKSSYPTAVIREGKITVSAKKLYEIVKE
MPDEEIHFTTKDNDWVEITCGKARFNIVGLSSEEFPYFPKVKEESFLVFKNEILADMIEKTSYAICYDETKYNLNGVFIK
AFDENGENVLKMVATDGHRLSVSQKAITGNITSELSKGVIFPKKGIFELKKMVEEEDGEIMLGFMDNSAVIKKGNTVVVM
RLIDGEFPDYTKVIPASNDRIVQINRDKFLHSLKRMAILSSEKFKGVKIEVNPELVIISSSNPELGEAREEIEVQYEGNN
ISARFNAKYLIDVLSVMEQREVELKLKDELSPVIMKAAAEEEFLAVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=32.7077747989276, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42282; Mature: 42282

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRISKEIFMKALQRIQGIVEKRNTMPILSNALLEVQNDQLFITATDLEVGMKSSYPTA
CCEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCEEEEEEECEEECCCCCCCCE
VIREGKITVSAKKLYEIVKEMPDEEIHFTTKDNDWVEITCGKARFNIVGLSSEEFPYFPK
EEECCEEEEEHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCEEEEEEECCCCCCCCCCC
VKEESFLVFKNEILADMIEKTSYAICYDETKYNLNGVFIKAFDENGENVLKMVATDGHRL
CCCCCEEEEHHHHHHHHHHHCCEEEEEECCEECCCEEEEEEECCCCCEEEEEEECCCCEE
SVSQKAITGNITSELSKGVIFPKKGIFELKKMVEEEDGEIMLGFMDNSAVIKKGNTVVVM
EEECCEEECCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCEEEECCCEEEEE
RLIDGEFPDYTKVIPASNDRIVQINRDKFLHSLKRMAILSSEKFKGVKIEVNPELVIISS
EEECCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEEC
SNPELGEAREEIEVQYEGNNISARFNAKYLIDVLSVMEQREVELKLKDELSPVIMKAAAE
CCCCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHCC
EEFLAVIMPMRL
CCEEEEEEECCC
>Mature Secondary Structure
MEFRISKEIFMKALQRIQGIVEKRNTMPILSNALLEVQNDQLFITATDLEVGMKSSYPTA
CCEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCEEEEEEECEEECCCCCCCCE
VIREGKITVSAKKLYEIVKEMPDEEIHFTTKDNDWVEITCGKARFNIVGLSSEEFPYFPK
EEECCEEEEEHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCEEEEEEECCCCCCCCCCC
VKEESFLVFKNEILADMIEKTSYAICYDETKYNLNGVFIKAFDENGENVLKMVATDGHRL
CCCCCEEEEHHHHHHHHHHHCCEEEEEECCEECCCEEEEEEECCCCCEEEEEEECCCCEE
SVSQKAITGNITSELSKGVIFPKKGIFELKKMVEEEDGEIMLGFMDNSAVIKKGNTVVVM
EEECCEEECCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCEEEECCCEEEEE
RLIDGEFPDYTKVIPASNDRIVQINRDKFLHSLKRMAILSSEKFKGVKIEVNPELVIISS
EEECCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEEC
SNPELGEAREEIEVQYEGNNISARFNAKYLIDVLSVMEQREVELKLKDELSPVIMKAAAE
CCCCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHCC
EEFLAVIMPMRL
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]