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The map label for this gene is dnaA [H]

Identifier: 197116403

GI number: 197116403

Start: 166

End: 1542

Strand: Direct

Name: dnaA [H]

Synonym: Gbem_0001

Alternate gene names: 197116403

Gene position: 166-1542 (Clockwise)

Preceding gene: NA

Following gene: 197116404

Centisome position: 0.0

GC content: 53.23

Gene sequence:

>1377_bases
ATGGAAAATATTTGGCTGGAAGCCCAGACAAATCTTAAGCAAGTATTAACCGAACAGACATACAGTACGTGGATCGACCC
ATTGAAGTTTCTGGGCGCCACCGTTGACACCATAGTCCTCGAAGTCCCCAGTTCATTCTTTCAAAAGTGGGTTACTGACA
AATATCTGGCCATGATCAAGGAAGCCATCTCCGCGGTCAACGGCAAAAGCTACCAGATAGAGTTCCATGTCGCCGAAGAG
AAGCCGGAGGCAGCTCACGAGGCAAAGCCCGAAAAAGAGGCCAAGCCTGCCAGGGAGAAAGAAAGGGATAAGGACAAGGA
AAAAGAGAAGGATAGAGAAAAGGAAAAGAAGGAGCTGGTTCCCAATCTGAACCCCAAGTACACCTTCGAGTCTTTCGTCT
CGGGTCCTAGCAACCAGTTCGCGTATGCAGCTTCCCAGGCGGTGGCGAACAAGCCGGCTACCAATTACAACCCGCTTTTC
ATCTACGGCGGTGTAGGTCTTGGAAAGACGCACCTGGTCAACGCCATCGGCAACCATATCCTGGCCAAGAACCCGAAGGC
GAAGATCTGCTATTACTCCTCCGAAAAGTTCATGAACGAGATGATCAACTCGCTCCGCTACAAGAAGATGGACGAGTTCC
GCAACAAGTTCCGGAAAATGGACCTGCTGCTCATCGACGACATACAGTTCATGGCCGGAAAAGAGGCCACGCAGGAAGAG
TTCTTCCACACCTTCAATGCGCTCTACGAGTCGCACAAGCAGATCGTGGTCACCTCCGATAAGTTCCCGAAGGACATCCC
AGGGCTCGAGGAGCGGTTGAGAAGCCGTTTCGAATGGGGGCTGATCGCGGACATCCAGCCACCGGGAGTGGAGACCAAAG
TCGCCATTCTCAAGAAGAAGTCCGACATGCACGCGGTCAACCTGCCCGACGACGTGGCTCTTTTTCTAGCGGAAGGCGCG
AACAGCAACATCCGCGAGTTGGAAGGGATGCTGATCAGGCTGGAGGCGTTTGCAAGCCTCACCGGCCAGGAGATAACGCT
CAGCATGGCCCGCGAGGTGATGAAGGACATCATCGTCGAGAAGACCCGCGACATCACCGTCGAGATGATACAGAAGACCG
TGGCGGAGCACTTCCGCATCAAGGTATCCGAACTGAAGTCCGACAAAAGGATCAAGACCCTCGTGGTACCGCGCCAGATA
GCGATCTACATCTGCCGCGAGCTGACCAAGGCGTCCTACCCGGAAATAGGCGAGAAGTTCGGGGGGAAGGACCACTCCAC
CATCATCCACTCGGTAAAAAAGATTGAGAAGCAGATCGCGGGTGACGATGAGTTTAAGGCGACTGTGGAAGACATAAGGA
AAAAGCTGTTCACTTAA

Upstream 100 bases:

>100_bases
TGTGCAAAACCCGATAACCTACTGTATTTACCTATCTTATTTATTATCAACAGTTGTTGATAAATCTGTTTAAAAACAGT
GAAAAAAAAGGCCTCTCTCC

Downstream 100 bases:

>100_bases
AGGGGAAAACTGTGCATAACTTTCCCCTTCAGCGCGGTGTCAAAAAGGCCTGTGCAGAAACAAGCCAAACTCAACAGATT
ATGCCTGTGACTTTCCAGAG

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MENIWLEAQTNLKQVLTEQTYSTWIDPLKFLGATVDTIVLEVPSSFFQKWVTDKYLAMIKEAISAVNGKSYQIEFHVAEE
KPEAAHEAKPEKEAKPAREKERDKDKEKEKDREKEKKELVPNLNPKYTFESFVSGPSNQFAYAASQAVANKPATNYNPLF
IYGGVGLGKTHLVNAIGNHILAKNPKAKICYYSSEKFMNEMINSLRYKKMDEFRNKFRKMDLLLIDDIQFMAGKEATQEE
FFHTFNALYESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPGVETKVAILKKKSDMHAVNLPDDVALFLAEGA
NSNIRELEGMLIRLEAFASLTGQEITLSMAREVMKDIIVEKTRDITVEMIQKTVAEHFRIKVSELKSDKRIKTLVVPRQI
AIYICRELTKASYPEIGEKFGGKDHSTIIHSVKKIEKQIAGDDEFKATVEDIRKKLFT

Sequences:

>Translated_458_residues
MENIWLEAQTNLKQVLTEQTYSTWIDPLKFLGATVDTIVLEVPSSFFQKWVTDKYLAMIKEAISAVNGKSYQIEFHVAEE
KPEAAHEAKPEKEAKPAREKERDKDKEKEKDREKEKKELVPNLNPKYTFESFVSGPSNQFAYAASQAVANKPATNYNPLF
IYGGVGLGKTHLVNAIGNHILAKNPKAKICYYSSEKFMNEMINSLRYKKMDEFRNKFRKMDLLLIDDIQFMAGKEATQEE
FFHTFNALYESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPGVETKVAILKKKSDMHAVNLPDDVALFLAEGA
NSNIRELEGMLIRLEAFASLTGQEITLSMAREVMKDIIVEKTRDITVEMIQKTVAEHFRIKVSELKSDKRIKTLVVPRQI
AIYICRELTKASYPEIGEKFGGKDHSTIIHSVKKIEKQIAGDDEFKATVEDIRKKLFT
>Mature_458_residues
MENIWLEAQTNLKQVLTEQTYSTWIDPLKFLGATVDTIVLEVPSSFFQKWVTDKYLAMIKEAISAVNGKSYQIEFHVAEE
KPEAAHEAKPEKEAKPAREKERDKDKEKEKDREKEKKELVPNLNPKYTFESFVSGPSNQFAYAASQAVANKPATNYNPLF
IYGGVGLGKTHLVNAIGNHILAKNPKAKICYYSSEKFMNEMINSLRYKKMDEFRNKFRKMDLLLIDDIQFMAGKEATQEE
FFHTFNALYESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPGVETKVAILKKKSDMHAVNLPDDVALFLAEGA
NSNIRELEGMLIRLEAFASLTGQEITLSMAREVMKDIIVEKTRDITVEMIQKTVAEHFRIKVSELKSDKRIKTLVVPRQI
AIYICRELTKASYPEIGEKFGGKDHSTIIHSVKKIEKQIAGDDEFKATVEDIRKKLFT

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=445, Percent_Identity=43.5955056179775, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI226510964, Length=234, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52408; Mature: 52408

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIWLEAQTNLKQVLTEQTYSTWIDPLKFLGATVDTIVLEVPSSFFQKWVTDKYLAMIK
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EAISAVNGKSYQIEFHVAEEKPEAAHEAKPEKEAKPAREKERDKDKEKEKDREKEKKELV
HHHHHCCCCEEEEEEEECCCCCCHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PNLNPKYTFESFVSGPSNQFAYAASQAVANKPATNYNPLFIYGGVGLGKTHLVNAIGNHI
CCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
LAKNPKAKICYYSSEKFMNEMINSLRYKKMDEFRNKFRKMDLLLIDDIQFMAGKEATQEE
CCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHHHHHCCCHHHHH
FFHTFNALYESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPGVETKVAILKKK
HHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCC
SDMHAVNLPDDVALFLAEGANSNIRELEGMLIRLEAFASLTGQEITLSMAREVMKDIIVE
CCCCEECCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KTRDITVEMIQKTVAEHFRIKVSELKSDKRIKTLVVPRQIAIYICRELTKASYPEIGEKF
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHCCCHHHHHHH
GGKDHSTIIHSVKKIEKQIAGDDEFKATVEDIRKKLFT
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENIWLEAQTNLKQVLTEQTYSTWIDPLKFLGATVDTIVLEVPSSFFQKWVTDKYLAMIK
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EAISAVNGKSYQIEFHVAEEKPEAAHEAKPEKEAKPAREKERDKDKEKEKDREKEKKELV
HHHHHCCCCEEEEEEEECCCCCCHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PNLNPKYTFESFVSGPSNQFAYAASQAVANKPATNYNPLFIYGGVGLGKTHLVNAIGNHI
CCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
LAKNPKAKICYYSSEKFMNEMINSLRYKKMDEFRNKFRKMDLLLIDDIQFMAGKEATQEE
CCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHHHHHCCCHHHHH
FFHTFNALYESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPGVETKVAILKKK
HHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCC
SDMHAVNLPDDVALFLAEGANSNIRELEGMLIRLEAFASLTGQEITLSMAREVMKDIIVE
CCCCEECCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KTRDITVEMIQKTVAEHFRIKVSELKSDKRIKTLVVPRQIAIYICRELTKASYPEIGEKF
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHCCCHHHHHHH
GGKDHSTIIHSVKKIEKQIAGDDEFKATVEDIRKKLFT
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA