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The map label for this gene is recF

Identifier: 197116405

GI number: 197116405

Start: 3017

End: 4111

Strand: Direct

Name: recF

Synonym: Gbem_0003

Alternate gene names: 197116405

Gene position: 3017-4111 (Clockwise)

Preceding gene: 197116404

Following gene: 197116406

Centisome position: 0.07

GC content: 58.45

Gene sequence:

>1095_bases
ATGAAGCTTATTAAGCTCAAACTTGCTTCCTTCCGCAACCTGCAGAACATAGAACTTGCGCCAGGCAAGAAGTTCAACGT
TTTCTACGGTAATAACGGCCAGGGCAAGACCAACCTGCTGGAGTCGATCTACCTCCTAGCTACCATGAAGTCGTTCAAGC
AGGCGCGAAATGCGGAGCTGATCGCGTTCGCAGGCGAGTTTGCGCTGGTGAAAGGGACCGTGGAACGGGACCAGGTCCGG
CGGGAAATAGCGGTCCTCATCGAGAAGCAGGGGAAAAAGGCGAAGGTCGACGCCAAGCTGATGACCAGGCTGGACGACTT
CTTCGGCAATTTGAACGTGGTGCTCTTCACCCCGGAAGAGATATCCATGGTGCGCGGGGGACCGGACCTCCGGCGCAGGT
ACCTGGACCGGGCGGTTTTCACCTGCGACCTCGGCTACCTGACCGCTTACCACGACTACGCAAAGATCCTGAAGAACCGC
AACGCCCTCCTCAAGGTGAACGAAACCACGGGGATTGAGGTCTGGACCGAGCAATTGGTACAGGCGGCGCTTTTGGTGAT
CGAGCGGAGAAAGGCGTACCTGGACCGGATTGGGAAGTTGCTGCAGGGGTTTTACAGCGAGATATCCGGCAACGACGAGA
CGGTTCAGATTGAATACCGGCTGCATGGGGTGGACGAGCGGCTTTTAGCCGAGGATCCGGCCGGGGCACTCAACCAGGCC
CTCAGGGCGCATGCGGCGGAGGAGAGAAGGCGCGGCACCACGGCCATCGGCCCCCACCGCGACGATCTTTACTTCGGCCT
CAACGGCCGCAGCGCCAGGCAGTTCGCTTCCCAGGGACAGCAGCGCTCCTTCGTGCTGGCGCTCAAGATGGCGGAGATAG
AACATATCACCAGGTGCTTCGAGGCGCCGCCGGTGCTCTTGCTGGACGACATGACCAGCGAGCTGGACCGGGAGCGCAAT
CGCAACCTGATGGAGTTTTTGAAGAAACGGGAGATGCAGGTATTCATCACCACGACGTCGCTCCATAACGTGGACATCGA
CGAACTGCAGGACAACAGGACGTTTCGCATCAAAGAGGGAAAAATTCTCGACTAG

Upstream 100 bases:

>100_bases
GAAACAACAGGCGGAGCTATTGATCATTTACTGTAACAGCCAGGTTTTCTTAATCTCAATCTCAATCTCAATCTTAATCT
TGAGTTAATCTAGATTTCAG

Downstream 100 bases:

>100_bases
GGGTAAAGATGAACTCAGAAGAACAGGGCGATTACGGGGCAGATAAGATCCAGATACTGGAAGGACTGGAAGCGGTACGA
AAACGTCCGGCCATGTACAT

Product: DNA replication/repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKLIKLKLASFRNLQNIELAPGKKFNVFYGNNGQGKTNLLESIYLLATMKSFKQARNAELIAFAGEFALVKGTVERDQVR
REIAVLIEKQGKKAKVDAKLMTRLDDFFGNLNVVLFTPEEISMVRGGPDLRRRYLDRAVFTCDLGYLTAYHDYAKILKNR
NALLKVNETTGIEVWTEQLVQAALLVIERRKAYLDRIGKLLQGFYSEISGNDETVQIEYRLHGVDERLLAEDPAGALNQA
LRAHAAEERRRGTTAIGPHRDDLYFGLNGRSARQFASQGQQRSFVLALKMAEIEHITRCFEAPPVLLLDDMTSELDRERN
RNLMEFLKKREMQVFITTTSLHNVDIDELQDNRTFRIKEGKILD

Sequences:

>Translated_364_residues
MKLIKLKLASFRNLQNIELAPGKKFNVFYGNNGQGKTNLLESIYLLATMKSFKQARNAELIAFAGEFALVKGTVERDQVR
REIAVLIEKQGKKAKVDAKLMTRLDDFFGNLNVVLFTPEEISMVRGGPDLRRRYLDRAVFTCDLGYLTAYHDYAKILKNR
NALLKVNETTGIEVWTEQLVQAALLVIERRKAYLDRIGKLLQGFYSEISGNDETVQIEYRLHGVDERLLAEDPAGALNQA
LRAHAAEERRRGTTAIGPHRDDLYFGLNGRSARQFASQGQQRSFVLALKMAEIEHITRCFEAPPVLLLDDMTSELDRERN
RNLMEFLKKREMQVFITTTSLHNVDIDELQDNRTFRIKEGKILD
>Mature_364_residues
MKLIKLKLASFRNLQNIELAPGKKFNVFYGNNGQGKTNLLESIYLLATMKSFKQARNAELIAFAGEFALVKGTVERDQVR
REIAVLIEKQGKKAKVDAKLMTRLDDFFGNLNVVLFTPEEISMVRGGPDLRRRYLDRAVFTCDLGYLTAYHDYAKILKNR
NALLKVNETTGIEVWTEQLVQAALLVIERRKAYLDRIGKLLQGFYSEISGNDETVQIEYRLHGVDERLLAEDPAGALNQA
LRAHAAEERRRGTTAIGPHRDDLYFGLNGRSARQFASQGQQRSFVLALKMAEIEHITRCFEAPPVLLLDDMTSELDRERN
RNLMEFLKKREMQVFITTTSLHNVDIDELQDNRTFRIKEGKILD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=367, Percent_Identity=28.6103542234332, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_GEOBB (B5E7P8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002136832.1
- ProteinModelPortal:   B5E7P8
- SMR:   B5E7P8
- GeneID:   6782822
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0003
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK827576
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41657; Mature: 41657

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIKLKLASFRNLQNIELAPGKKFNVFYGNNGQGKTNLLESIYLLATMKSFKQARNAEL
CCEEEEEHHHHCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
IAFAGEFALVKGTVERDQVRREIAVLIEKQGKKAKVDAKLMTRLDDFFGNLNVVLFTPEE
EEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHH
ISMVRGGPDLRRRYLDRAVFTCDLGYLTAYHDYAKILKNRNALLKVNETTGIEVWTEQLV
HHHHCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
QAALLVIERRKAYLDRIGKLLQGFYSEISGNDETVQIEYRLHGVDERLLAEDPAGALNQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHCCCCCHHHHHH
LRAHAAEERRRGTTAIGPHRDDLYFGLNGRSARQFASQGQQRSFVLALKMAEIEHITRCF
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
EAPPVLLLDDMTSELDRERNRNLMEFLKKREMQVFITTTSLHNVDIDELQDNRTFRIKEG
CCCCEEEEHHHHHHHHHHHCCHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCEEEEECC
KILD
CCCC
>Mature Secondary Structure
MKLIKLKLASFRNLQNIELAPGKKFNVFYGNNGQGKTNLLESIYLLATMKSFKQARNAEL
CCEEEEEHHHHCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
IAFAGEFALVKGTVERDQVRREIAVLIEKQGKKAKVDAKLMTRLDDFFGNLNVVLFTPEE
EEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHH
ISMVRGGPDLRRRYLDRAVFTCDLGYLTAYHDYAKILKNRNALLKVNETTGIEVWTEQLV
HHHHCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
QAALLVIERRKAYLDRIGKLLQGFYSEISGNDETVQIEYRLHGVDERLLAEDPAGALNQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHCCCCCHHHHHH
LRAHAAEERRRGTTAIGPHRDDLYFGLNGRSARQFASQGQQRSFVLALKMAEIEHITRCF
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
EAPPVLLLDDMTSELDRERNRNLMEFLKKREMQVFITTTSLHNVDIDELQDNRTFRIKEG
CCCCEEEEHHHHHHHHHHHCCHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCEEEEECC
KILD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA