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The map label for this gene is rpoD [H]

Identifier: 197106036

GI number: 197106036

Start: 2960031

End: 2961947

Strand: Reverse

Name: rpoD [H]

Synonym: PHZ_c2575

Alternate gene names: 197106036

Gene position: 2961947-2960031 (Counterclockwise)

Preceding gene: 197106037

Following gene: 197106035

Centisome position: 74.12

GC content: 66.51

Gene sequence:

>1917_bases
ATGAGCTCGAACACAGCCGAAGCCGAAACGCCCGAAACCACCCAAGGCGACGGCCCGCTGCTCGACCTGACCGACGCCGC
GGTCAAGAAGTTCATCAAGCAGGCCAAGGCCCGCGGCTACGTCACCATGGACGAGCTGAACAAGGTGATGCCCTCGGAAG
AGGTCACCTCGGAGGCCATCGAAGACACCCTGGCGATGCTCAACGAAATGGGCGTCAACGTCGTGGAGTCCGAAGAGGAC
GCCGAAGGCGGCGAGGTCGCGGTCCGCGAGGAGAGCGCCGTCGTCGAGACGACGAAGGAGCCGGCCTACGACCGCACGGA
CGATCCGGTGCGGATGTACCTGCGCGAGATGGGCTCGGTCGAGCTTCTGTCGCGCGAGGGCGAGATCGCGATCGCCAAGC
GCATCGAGGCCGGCCGCGACACCATGATCCGCGGGCTGTGCGAGAGCGCGCTGACCTTCGAGGCCATCATGGTGTGGCGC
GAGGAGCTGGAGGGCGGCCGCATCCTGCTGCGCGAGGTGATCGACCTCGAACAGACCTACGGCGGCCAGAACCAGGGCGA
GGCGCCCGCCGAGGCGGCGGAAGCCGAGGAGCCCGAGGCCGAAGGCGCCGAGGGCGAAGGCGAGAGCGACGACGACTTCG
ACGACGGCGCCGGCCCGACCATCAGCGCCATGGAGGCCGAGCTGCGCGAAGGGGTCATGGCGACCCTCGACGCGATCGCC
GCCGAGTTCGAGAGCTTCCGCCTGCTGCAGGAGAAGCTGGTCGCCGCCAAGCTGAAGGGCGAGGACCTCTCGGAAGGCGA
CCGCAAGGCCTACCAGGCCGCCACGGGCGCCATCGTCCAGCACCTCAAGACCCTGAAGCTGAACAACAACCGCATCGAGG
CGCTGGTCGAGCAGCTCTATGCGATCAACAAGCGCCTGATGGTGCTGGAAGGCCGCCTGCTGCGCCTGGCCGACAGCTAC
GGCATCAGCCGCCAGGAGTTCCTGAAGGCCTACTTCGGCAACGAGATGCGCCCGGACTGGACCGACATGGTCAAGCCGCT
GGGCGTGCGCTGGACGAAGTTCGCCGAGAACGACGCCTCCCAGATCGGCGAGATTCGCAACGAGATCGCCGCGCTGGCGC
AGGAAACCGGCGTGCCGATCGACGACTACCGCCGCATCGTCCAGACGGTGCAGAAGGGCGAGCGCGAGGCCCGTCAGGCG
AAGAAGGAGATGGTCGAGGCCAACCTGCGCCTCGTGATCTCGATCGCCAAGAAATACACGAACCGCGGCCTGCAGTTCCT
GGACCTGATCCAGGAGGGCAACATCGGCCTGATGAAGGCGGTGGACAAGTTCGAGTACCGCCGCGGCTACAAGTTCTCGA
CCTACGCCACCTGGTGGATTCGGCAGGCGATCACCCGCTCGATCGCAGACCAGGCCCGGACCATCCGCATCCCGGTGCAC
ATGATCGAGACGATCAACAAGATCGTCCGCACCAGCCGCCAGATGCTGCACGAGATCGGCCGCGAGCCGACCCCGGAGGA
GCTGGCCGAGAAGCTGGCCATGCCGCTGGAGAAGGTGCGCAAGGTCCTGAAGATCGCCAAGGAGCCGATCTCGCTCGAGA
CCCCCATCGGTGACGAGGAGGATTCGCACCTCGGCGACTTCATCGAGGACAAGAACGCCGTCCTGCCGATCGATGCGGCC
ATCCAGTCGAACCTGCGCGAGACCACCACCCGCGTGCTCGCCTCGCTGACGCCGCGCGAGGAGCGCGTGCTGCGGATGCG
CTTCGGCATCGGCATGAACACCGACCACACGCTGGAAGAAGTCGGCCAGCAGTTCTCGGTCACCCGCGAACGAATCCGCC
AGATCGAGGCCAAGGCGCTGCGCAAGCTGAAGCACCCGAGCCGCTCGCGGAAGCTGCGCAGCTTCCTCGACAGCTAG

Upstream 100 bases:

>100_bases
ATACGGGTCGTAACGTCGCGGCCCCTAAGCATTTGAACGCCGCACATTATCTCGGGCCCGCGTCTTGCAGGAGACGGGGA
CGCCCATGCCGGAGAACTCG

Downstream 100 bases:

>100_bases
GACTGGCGCGCACGCCGCGATCCATTAGAACTCCCCGGGACAGGCGTTCCGGGGAGTTTTTCATGAAGCCGCTCAAGATG
TTGGCCGTCCCGGCCGTGGC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSSNTAEAETPETTQGDGPLLDLTDAAVKKFIKQAKARGYVTMDELNKVMPSEEVTSEAIEDTLAMLNEMGVNVVESEED
AEGGEVAVREESAVVETTKEPAYDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEAIMVWR
EELEGGRILLREVIDLEQTYGGQNQGEAPAEAAEAEEPEAEGAEGEGESDDDFDDGAGPTISAMEAELREGVMATLDAIA
AEFESFRLLQEKLVAAKLKGEDLSEGDRKAYQAATGAIVQHLKTLKLNNNRIEALVEQLYAINKRLMVLEGRLLRLADSY
GISRQEFLKAYFGNEMRPDWTDMVKPLGVRWTKFAENDASQIGEIRNEIAALAQETGVPIDDYRRIVQTVQKGEREARQA
KKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
MIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPIDAA
IQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS

Sequences:

>Translated_638_residues
MSSNTAEAETPETTQGDGPLLDLTDAAVKKFIKQAKARGYVTMDELNKVMPSEEVTSEAIEDTLAMLNEMGVNVVESEED
AEGGEVAVREESAVVETTKEPAYDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEAIMVWR
EELEGGRILLREVIDLEQTYGGQNQGEAPAEAAEAEEPEAEGAEGEGESDDDFDDGAGPTISAMEAELREGVMATLDAIA
AEFESFRLLQEKLVAAKLKGEDLSEGDRKAYQAATGAIVQHLKTLKLNNNRIEALVEQLYAINKRLMVLEGRLLRLADSY
GISRQEFLKAYFGNEMRPDWTDMVKPLGVRWTKFAENDASQIGEIRNEIAALAQETGVPIDDYRRIVQTVQKGEREARQA
KKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
MIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPIDAA
IQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS
>Mature_637_residues
SSNTAEAETPETTQGDGPLLDLTDAAVKKFIKQAKARGYVTMDELNKVMPSEEVTSEAIEDTLAMLNEMGVNVVESEEDA
EGGEVAVREESAVVETTKEPAYDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEAIMVWRE
ELEGGRILLREVIDLEQTYGGQNQGEAPAEAAEAEEPEAEGAEGEGESDDDFDDGAGPTISAMEAELREGVMATLDAIAA
EFESFRLLQEKLVAAKLKGEDLSEGDRKAYQAATGAIVQHLKTLKLNNNRIEALVEQLYAINKRLMVLEGRLLRLADSYG
ISRQEFLKAYFGNEMRPDWTDMVKPLGVRWTKFAENDASQIGEIRNEIAALAQETGVPIDDYRRIVQTVQKGEREARQAK
KEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHM
IETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPIDAAI
QSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=633, Percent_Identity=48.9731437598736, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=42.6666666666667, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=26.4462809917355, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71736; Mature: 71605

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNTAEAETPETTQGDGPLLDLTDAAVKKFIKQAKARGYVTMDELNKVMPSEEVTSEAI
CCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEHHHHHHHCCCHHHHHHHH
EDTLAMLNEMGVNVVESEEDAEGGEVAVREESAVVETTKEPAYDRTDDPVRMYLREMGSV
HHHHHHHHHHCCCEECCCCCCCCCCEEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHCCH
ELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEAIMVWREELEGGRILLREVIDLEQTY
HHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GGQNQGEAPAEAAEAEEPEAEGAEGEGESDDDFDDGAGPTISAMEAELREGVMATLDAIA
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEFESFRLLQEKLVAAKLKGEDLSEGDRKAYQAATGAIVQHLKTLKLNNNRIEALVEQLY
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AINKRLMVLEGRLLRLADSYGISRQEFLKAYFGNEMRPDWTDMVKPLGVRWTKFAENDAS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHCCHHH
QIGEIRNEIAALAQETGVPIDDYRRIVQTVQKGEREARQAKKEMVEANLRLVISIAKKYT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
HCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHH
MIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DSHLGDFIEDKNAVLPIDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEE
CCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHH
VGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSNTAEAETPETTQGDGPLLDLTDAAVKKFIKQAKARGYVTMDELNKVMPSEEVTSEAI
CCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEHHHHHHHCCCHHHHHHHH
EDTLAMLNEMGVNVVESEEDAEGGEVAVREESAVVETTKEPAYDRTDDPVRMYLREMGSV
HHHHHHHHHHCCCEECCCCCCCCCCEEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHCCH
ELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEAIMVWREELEGGRILLREVIDLEQTY
HHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GGQNQGEAPAEAAEAEEPEAEGAEGEGESDDDFDDGAGPTISAMEAELREGVMATLDAIA
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEFESFRLLQEKLVAAKLKGEDLSEGDRKAYQAATGAIVQHLKTLKLNNNRIEALVEQLY
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AINKRLMVLEGRLLRLADSYGISRQEFLKAYFGNEMRPDWTDMVKPLGVRWTKFAENDAS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHCCHHH
QIGEIRNEIAALAQETGVPIDDYRRIVQTVQKGEREARQAKKEMVEANLRLVISIAKKYT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
HCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHH
MIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DSHLGDFIEDKNAVLPIDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEE
CCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHH
VGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7543475; 11259647 [H]