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The map label for this gene is dnaG [H]

Identifier: 197106037

GI number: 197106037

Start: 2962380

End: 2964293

Strand: Reverse

Name: dnaG [H]

Synonym: PHZ_c2576

Alternate gene names: 197106037

Gene position: 2964293-2962380 (Counterclockwise)

Preceding gene: 197106038

Following gene: 197106036

Centisome position: 74.18

GC content: 72.1

Gene sequence:

>1914_bases
ATGGTCGGGCGGTTCGACGAGCGCTTCATCGAAGAGATCAAGGGACGGCTGCGGCCGTCCGACGTGATCGGCAAGACCGT
GAAGCTGCGCAAGCAGGGCCGCGAGTACGTGGGCCTGTCGCCGTTCAACAAGGAGAAGACCCCCTCGTTCTTCGTGAACG
ACGAGAAGGGCCAGTTCTTCGATTTCTCGTCGGGCAAGACCGGCGACATCATCACCTTCCTGCAGGAGACCGAGCGGCTC
TCGTTCGCCGAGGCGGTGGAAAAGCTGGCGGCCGAGGCGGGGCTGGCGCTGCCGGCGGCAGACCCGCGCGGCGCCGAGGT
CGAGAAGAAGCGCCAGGGCCTCGCCGACTGGCTGGAGCTGGCCGCCCAGTGGTTCGAGGCCGAGCTGAAACGGCCGGTGG
GCCGCGACGCCCGCGCATACCTCGAGAAGCGCGGCCTGCCCGAGAAGGAATGGGCGAGGTTCCGCCTGGGCTTCTCGCCT
TCGGGCCGCACCGCGCTGAAGGACTATCTGGTGACCAAGGGCGCCCGTCCGGCCGAGCTGGTCGAGGCGGGCCTGCTGAT
CGCCCCCGAGGACGGCGGCGCGCCCTACGACCGCTTCCGCGACCGGATCATCTTCCCGATCCTGGACGCCCGCGGCCGGG
TGGTCTCGTTCGGCGGCCGGGCGATGGACCCGAACGCCCGCGCCAAGTACCTGAACGGCCCCGAGACGGTGGTCTTCCAC
AAGGGGAACAACCTCTACGGCCTCTCCGAGGCGCGGAAGATCCTGGCCGCGGCGCCGACCGGCGACGAGCCGCCGCTGGT
CGTGGTGGAAGGCTACATGGACGTGATCGCCTGCCAACGGGCGGGCATTCCGTCCGTCGCGCCCATGGGCACGGCGCTGA
CCGAGGAGCAGATGGAGGTGCTGTGGCGCCACCATCCCGAACCGACGCTGTGCTTCGACGGCGACCGGGCGGGCCGGCAG
GCCGCCGGGCGGGCCATCGACCGGGCGCTGCCGCTGCTGAAGCCCGGCAAGAGCTTCAAGTTCGCCCTGGTCGAGGGGGG
CAAGGACCCCGACGACGTGCTGCGCGAGCAGGGCCCCGCGGCCTTGAAGTCGCAGCTGGAGAAGACCACGCCGTTCGCCG
AGGCGCTGTTCGTGCGCGAGCGCGACCTGGAGCCGCTGGACACGCCCGAGCGGCGCACCGCGCTGAAGGTGCGCCTGCGC
AAGCTCGCCGCCACGATCGCCGATCCGGACCTGGCGCAGGCCTACAAGGAGGACCTGCTGGGCCGGTTCGAGCAGCTGTG
GCCCACCCAGCAGCCGGTCTACACCGTGGGCGCGGCCGGCCGGGAGCTGTCGCGTCACCGCTGGGCCAAGAAGGGCGCGC
TGGCCGGCGCGAGCCCCGAGACCAAGCAGGCGGCCCAGGACCTGAAGGCCTCGCCCCGGCCGCTCGCGGCGGCCCTGGCG
GTCGCGGCGCTGCACGATCCCAGCCTGATCGACAACTCCATCGAGCTGGTGGGGGCCCGCGGCTTCGGCGACGAACGGCT
GGACGCCATCGCCCAGGCGCTGGTGCAGATCCGCTACGAGACCGACGAGGCCGAGTTCGACGGCGTGGTGCGCCGCCTGC
GCTCCCGCGGGGTGTCGCAGGAGCTGATCGCCAAGCTGGAGCGGGACGCCCGGCGCGCCGGCGTGAGCGCCCCGTTCCTC
GACGCCACGGCCGCCCGCGACCGGGTCCGGGCGCTGTGGAGCCAGGCGTTCGAGCTCCTGATGAAGCTGGAGGCCCTGGA
GCGGGCGGTGGCGAGCGCCGTGGGCGACATGGCCCGCGATCCCAGCGCCGCCGCGGCCCTGATCAACCTGAAGGCCGAGC
GGGACCACCTGCGCAAGCTGGTCAACTCGGACTGGGCCGGCGAGGCGCACGACAAGCCGGTGCTGCCCCATTGA

Upstream 100 bases:

>100_bases
CCGCTAGCGACCGACTCGTCGCACCGTTTCGGCGCTATCATCCACAGGCGCGCGCGACTCATCCACAGGCCCTCGCCGCG
CAAGGTGGTAGCGGTTTCCC

Downstream 100 bases:

>100_bases
GGGGTGCGGCGAATCCGCACTGGCACGGGGACTGCCGCAAATCCTCGAACGAATTCATTCGCCTCGGCCGGACGCGGGGG
CGCCTTGACTTGCGGGACTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MVGRFDERFIEEIKGRLRPSDVIGKTVKLRKQGREYVGLSPFNKEKTPSFFVNDEKGQFFDFSSGKTGDIITFLQETERL
SFAEAVEKLAAEAGLALPAADPRGAEVEKKRQGLADWLELAAQWFEAELKRPVGRDARAYLEKRGLPEKEWARFRLGFSP
SGRTALKDYLVTKGARPAELVEAGLLIAPEDGGAPYDRFRDRIIFPILDARGRVVSFGGRAMDPNARAKYLNGPETVVFH
KGNNLYGLSEARKILAAAPTGDEPPLVVVEGYMDVIACQRAGIPSVAPMGTALTEEQMEVLWRHHPEPTLCFDGDRAGRQ
AAGRAIDRALPLLKPGKSFKFALVEGGKDPDDVLREQGPAALKSQLEKTTPFAEALFVRERDLEPLDTPERRTALKVRLR
KLAATIADPDLAQAYKEDLLGRFEQLWPTQQPVYTVGAAGRELSRHRWAKKGALAGASPETKQAAQDLKASPRPLAAALA
VAALHDPSLIDNSIELVGARGFGDERLDAIAQALVQIRYETDEAEFDGVVRRLRSRGVSQELIAKLERDARRAGVSAPFL
DATAARDRVRALWSQAFELLMKLEALERAVASAVGDMARDPSAAAALINLKAERDHLRKLVNSDWAGEAHDKPVLPH

Sequences:

>Translated_637_residues
MVGRFDERFIEEIKGRLRPSDVIGKTVKLRKQGREYVGLSPFNKEKTPSFFVNDEKGQFFDFSSGKTGDIITFLQETERL
SFAEAVEKLAAEAGLALPAADPRGAEVEKKRQGLADWLELAAQWFEAELKRPVGRDARAYLEKRGLPEKEWARFRLGFSP
SGRTALKDYLVTKGARPAELVEAGLLIAPEDGGAPYDRFRDRIIFPILDARGRVVSFGGRAMDPNARAKYLNGPETVVFH
KGNNLYGLSEARKILAAAPTGDEPPLVVVEGYMDVIACQRAGIPSVAPMGTALTEEQMEVLWRHHPEPTLCFDGDRAGRQ
AAGRAIDRALPLLKPGKSFKFALVEGGKDPDDVLREQGPAALKSQLEKTTPFAEALFVRERDLEPLDTPERRTALKVRLR
KLAATIADPDLAQAYKEDLLGRFEQLWPTQQPVYTVGAAGRELSRHRWAKKGALAGASPETKQAAQDLKASPRPLAAALA
VAALHDPSLIDNSIELVGARGFGDERLDAIAQALVQIRYETDEAEFDGVVRRLRSRGVSQELIAKLERDARRAGVSAPFL
DATAARDRVRALWSQAFELLMKLEALERAVASAVGDMARDPSAAAALINLKAERDHLRKLVNSDWAGEAHDKPVLPH
>Mature_637_residues
MVGRFDERFIEEIKGRLRPSDVIGKTVKLRKQGREYVGLSPFNKEKTPSFFVNDEKGQFFDFSSGKTGDIITFLQETERL
SFAEAVEKLAAEAGLALPAADPRGAEVEKKRQGLADWLELAAQWFEAELKRPVGRDARAYLEKRGLPEKEWARFRLGFSP
SGRTALKDYLVTKGARPAELVEAGLLIAPEDGGAPYDRFRDRIIFPILDARGRVVSFGGRAMDPNARAKYLNGPETVVFH
KGNNLYGLSEARKILAAAPTGDEPPLVVVEGYMDVIACQRAGIPSVAPMGTALTEEQMEVLWRHHPEPTLCFDGDRAGRQ
AAGRAIDRALPLLKPGKSFKFALVEGGKDPDDVLREQGPAALKSQLEKTTPFAEALFVRERDLEPLDTPERRTALKVRLR
KLAATIADPDLAQAYKEDLLGRFEQLWPTQQPVYTVGAAGRELSRHRWAKKGALAGASPETKQAAQDLKASPRPLAAALA
VAALHDPSLIDNSIELVGARGFGDERLDAIAQALVQIRYETDEAEFDGVVRRLRSRGVSQELIAKLERDARRAGVSAPFL
DATAARDRVRALWSQAFELLMKLEALERAVASAVGDMARDPSAAAALINLKAERDHLRKLVNSDWAGEAHDKPVLPH

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=427, Percent_Identity=40.5152224824356, Blast_Score=289, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70120; Mature: 70120

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGRFDERFIEEIKGRLRPSDVIGKTVKLRKQGREYVGLSPFNKEKTPSFFVNDEKGQFF
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCEE
DFSSGKTGDIITFLQETERLSFAEAVEKLAAEAGLALPAADPRGAEVEKKRQGLADWLEL
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAQWFEAELKRPVGRDARAYLEKRGLPEKEWARFRLGFSPSGRTALKDYLVTKGARPAEL
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHH
VEAGLLIAPEDGGAPYDRFRDRIIFPILDARGRVVSFGGRAMDPNARAKYLNGPETVVFH
HHCCCEEECCCCCCCHHHHHHCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCEEEEE
KGNNLYGLSEARKILAAAPTGDEPPLVVVEGYMDVIACQRAGIPSVAPMGTALTEEQMEV
CCCCEECHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LWRHHPEPTLCFDGDRAGRQAAGRAIDRALPLLKPGKSFKFALVEGGKDPDDVLREQGPA
HHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHCCCCH
ALKSQLEKTTPFAEALFVRERDLEPLDTPERRTALKVRLRKLAATIADPDLAQAYKEDLL
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GRFEQLWPTQQPVYTVGAAGRELSRHRWAKKGALAGASPETKQAAQDLKASPRPLAAALA
HHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
VAALHDPSLIDNSIELVGARGFGDERLDAIAQALVQIRYETDEAEFDGVVRRLRSRGVSQ
HHHHCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
ELIAKLERDARRAGVSAPFLDATAARDRVRALWSQAFELLMKLEALERAVASAVGDMARD
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSAAAALINLKAERDHLRKLVNSDWAGEAHDKPVLPH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVGRFDERFIEEIKGRLRPSDVIGKTVKLRKQGREYVGLSPFNKEKTPSFFVNDEKGQFF
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCEE
DFSSGKTGDIITFLQETERLSFAEAVEKLAAEAGLALPAADPRGAEVEKKRQGLADWLEL
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAQWFEAELKRPVGRDARAYLEKRGLPEKEWARFRLGFSPSGRTALKDYLVTKGARPAEL
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHH
VEAGLLIAPEDGGAPYDRFRDRIIFPILDARGRVVSFGGRAMDPNARAKYLNGPETVVFH
HHCCCEEECCCCCCCHHHHHHCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCEEEEE
KGNNLYGLSEARKILAAAPTGDEPPLVVVEGYMDVIACQRAGIPSVAPMGTALTEEQMEV
CCCCEECHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LWRHHPEPTLCFDGDRAGRQAAGRAIDRALPLLKPGKSFKFALVEGGKDPDDVLREQGPA
HHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHCCCCH
ALKSQLEKTTPFAEALFVRERDLEPLDTPERRTALKVRLRKLAATIADPDLAQAYKEDLL
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GRFEQLWPTQQPVYTVGAAGRELSRHRWAKKGALAGASPETKQAAQDLKASPRPLAAALA
HHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
VAALHDPSLIDNSIELVGARGFGDERLDAIAQALVQIRYETDEAEFDGVVRRLRSRGVSQ
HHHHCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
ELIAKLERDARRAGVSAPFLDATAARDRVRALWSQAFELLMKLEALERAVASAVGDMARD
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSAAAALINLKAERDHLRKLVNSDWAGEAHDKPVLPH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]