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The map label for this gene is putR [H]

Identifier: 197105868

GI number: 197105868

Start: 2740218

End: 2740697

Strand: Reverse

Name: putR [H]

Synonym: PHZ_c2407

Alternate gene names: 197105868

Gene position: 2740697-2740218 (Counterclockwise)

Preceding gene: 197105869

Following gene: 197105867

Centisome position: 68.58

GC content: 63.33

Gene sequence:

>480_bases
ATGGACGCGACGGGTGAGCTCGATGAGATGGACCTGCGCATTCTGGACGTGATCCAGAGGCATCCGCCGATGTCGACCGC
GGATCTCGCGGACAAGGTCGGCCTGTCCCAATCCCCGTGCTGGCGGCGCCTGACCCGCCTGAGGGAGGAAGGCTACATCC
TGGATCAGGTGACCCGCCTGAATGCGGAAAAGCTAGGCTTCCATACCGTCGTCTTCGCGAGTGTCCGGCTGTCGGCCCAC
GGCCGCACCAACCTCAGTGAGTTCGTGGAGGCGGTCTGCCGGATCCCGGAGGTCATGGACTGCTACCCGACGATGGGGCC
CTTCGACTTCCTGCTCCGCATCGTCACCCGCGACGTCGAGAGCTATCGGCAGTTCGTGTTCGGTCGCCTGCTCACCATGC
CGACGGTCCAGGAGGTCAACTCCACCATGACCATGGCGAGCGACAAGAGCTCCACGGCTCTGCCAATTCGTTTTCGGTAA

Upstream 100 bases:

>100_bases
CGCCTCTGCGTGTCCGAACAGCGGTCGGCGTCGCTGGGACCGCCAGGCTCCCGGCGCCTGCGCCTGACGACTGCCTTGCT
GAACGACGGGGGTCAGGAGC

Downstream 100 bases:

>100_bases
GCCGAGCGTTGGTTCGCGAATTGGCCAATTCGCCCGATCGCGGTTTCGACGGGTTGATGGCGCCCTGCAAGGCGACATCC
CATCACCGCGCGACGCAACG

Product: transcriptional regulator, AsnC family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MDATGELDEMDLRILDVIQRHPPMSTADLADKVGLSQSPCWRRLTRLREEGYILDQVTRLNAEKLGFHTVVFASVRLSAH
GRTNLSEFVEAVCRIPEVMDCYPTMGPFDFLLRIVTRDVESYRQFVFGRLLTMPTVQEVNSTMTMASDKSSTALPIRFR

Sequences:

>Translated_159_residues
MDATGELDEMDLRILDVIQRHPPMSTADLADKVGLSQSPCWRRLTRLREEGYILDQVTRLNAEKLGFHTVVFASVRLSAH
GRTNLSEFVEAVCRIPEVMDCYPTMGPFDFLLRIVTRDVESYRQFVFGRLLTMPTVQEVNSTMTMASDKSSTALPIRFR
>Mature_159_residues
MDATGELDEMDLRILDVIQRHPPMSTADLADKVGLSQSPCWRRLTRLREEGYILDQVTRLNAEKLGFHTVVFASVRLSAH
GRTNLSEFVEAVCRIPEVMDCYPTMGPFDFLLRIVTRDVESYRQFVFGRLLTMPTVQEVNSTMTMASDKSSTALPIRFR

Specific function: Transcriptional activator of the putA gene in response to proline [H]

COG id: COG1522

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH asnC-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081742, Length=151, Percent_Identity=32.4503311258278, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787116, Length=154, Percent_Identity=31.1688311688312, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011008
- InterPro:   IPR000485
- InterPro:   IPR019885
- InterPro:   IPR019888
- InterPro:   IPR019887
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01037 AsnC_trans_reg [H]

EC number: NA

Molecular weight: Translated: 18144; Mature: 18144

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00519 HTH_ASNC_1 ; PS50956 HTH_ASNC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDATGELDEMDLRILDVIQRHPPMSTADLADKVGLSQSPCWRRLTRLREEGYILDQVTRL
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHH
NAEKLGFHTVVFASVRLSAHGRTNLSEFVEAVCRIPEVMDCYPTMGPFDFLLRIVTRDVE
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHH
SYRQFVFGRLLTMPTVQEVNSTMTMASDKSSTALPIRFR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEC
>Mature Secondary Structure
MDATGELDEMDLRILDVIQRHPPMSTADLADKVGLSQSPCWRRLTRLREEGYILDQVTRL
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHH
NAEKLGFHTVVFASVRLSAHGRTNLSEFVEAVCRIPEVMDCYPTMGPFDFLLRIVTRDVE
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHH
SYRQFVFGRLLTMPTVQEVNSTMTMASDKSSTALPIRFR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606160 [H]