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The map label for this gene is murF [H]

Identifier: 197105789

GI number: 197105789

Start: 2639681

End: 2641078

Strand: Reverse

Name: murF [H]

Synonym: PHZ_c2327

Alternate gene names: 197105789

Gene position: 2641078-2639681 (Counterclockwise)

Preceding gene: 197105790

Following gene: 197105788

Centisome position: 66.09

GC content: 73.53

Gene sequence:

>1398_bases
ATGGCTGAGCCCCTCTGGACCGCCGAGGAGATCGCGGCCGCGACCGGCGGCAAGCTGGCGGGCCAGGCGTTCCGCGCGGG
CGGCGTCTCCATCGACACCCGCACGATCCAGCCGGGCGACCTGTTCGTGGCGCTGGCCGGCGTGCGCGACGGCCACGAGT
TCGTGGAGCAGGCCTTCGAGAAGGGCGCGGCCGGCGTCATCGCCTCGCGCCAGGTGTTCGGCCCGGCGGTGATGGTGCCC
GACACCCTGCACGCGCTGGAGAAGCTGGGCGCCGCCGCCCGCGAGCGCGCGCCGCAGGCCCGGCGCGGTGCGGTCACCGG
TTCGGTCGGCAAGACCAGCGTCACCCAGGCGATCATGGCCGGCCTGACCCTGGCCGGCCCGGCGCACTCGTCGGTGAAGT
CCTACAACAACCACATCGGCGTGCCGCTGACCCTGGCGCGGATGCCGCGGGGCACCGCCCGCGCGGTGTTCGAGATCGGC
ATGAACCACGCCGATGAGATCCGGCCGCTGAGCAGGATGGTCCGGCCGCACGCCGTCGTGATCACGACGGTGGGGCCCGT
CCACACCGAGAACTTCCCTGACGGGGAGGCGGGCGTGGCGCGCGCCAAGGCCGAGATCTTCGAGGGGCTGGAGCCCGGCG
GCGTCGCCGTTCTGAACGCCGACAACCGCTGGTTCGACCTGCTGAAGGGCGAGGCCGAGCGGGCCGGCGCGACGGTGCGC
ACGTTCGGGACGGGCGAGGCGTGCGACGCGCGGCTGATCGACTTCCAGGTTCCCGCGGGCCACGCGGTCGTGCAGGCCCG
CCTGCACGGCAAGGCGCTCGACTTCCCGATCCTGCAGACCGGCCTGCACTGGGGCCTGAACAGCATGGCCGTGCTGCTGA
TGCTCGAGGCGCTGGGCGTCGACCTGGACGACGGTCTCGCCGCCCTGGGCTCGTTCGAGCCGCTGGCCGGCCGCGGCGCC
GAGGCGCAGGTCCGCAAGGGCGCCGGCGCCTTCACGCTGATCGACGAGAGCTACAACGCCAACCCGATCTCCATGGCCTC
GGCCATCGCCACCCTGGGCGCGCGGGAGACCGCCGGTCGGCGCATCGTGGCCCTGACCGACATGCTCGAACTGGGGCCGG
AGGCTGAGGCCTTCCACCGCGGCCTCGCCGAGCCGCTGGAGGCTGCGGCGATTGACCTCGTCTTCTGCGCCGGCCCGCTG
ATGAAATCCCTCTGGGACGCCCTTCCGCCGACTCGCCGCGGCGGCTACGCCGAGTCCGCGGCCGAACTCGCGCCGCAGGT
CGTCCAGGCCGTAGAGCCCGGCGACGTGGTGATGGTGAAGGGGTCGAACGGATCGAAGGCGGGCGCGATCGCGGCCGCCC
TGTCCGCGCTCGATTCCCGGTCGGGGGAGGGCGCCTGA

Upstream 100 bases:

>100_bases
GTGGTCGCCGGCAAGGGCCACGAGCAGGGCCAGATCGTGGGCGGCGTGACCCACCCCTTCGATGACGTGGCCGAGACGGC
CCAGGCGCTGGAGCTGGTCC

Downstream 100 bases:

>100_bases
TGTTCTACTGGCTGTACGAACAGTTCGCGGCGTCGGGCTATGTCCCGATCCTCAACCTGCTGAAATACCAGACCTTCCGG
ACCGGCCTTGCGATCTTCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAEPLWTAEEIAAATGGKLAGQAFRAGGVSIDTRTIQPGDLFVALAGVRDGHEFVEQAFEKGAAGVIASRQVFGPAVMVP
DTLHALEKLGAAARERAPQARRGAVTGSVGKTSVTQAIMAGLTLAGPAHSSVKSYNNHIGVPLTLARMPRGTARAVFEIG
MNHADEIRPLSRMVRPHAVVITTVGPVHTENFPDGEAGVARAKAEIFEGLEPGGVAVLNADNRWFDLLKGEAERAGATVR
TFGTGEACDARLIDFQVPAGHAVVQARLHGKALDFPILQTGLHWGLNSMAVLLMLEALGVDLDDGLAALGSFEPLAGRGA
EAQVRKGAGAFTLIDESYNANPISMASAIATLGARETAGRRIVALTDMLELGPEAEAFHRGLAEPLEAAAIDLVFCAGPL
MKSLWDALPPTRRGGYAESAAELAPQVVQAVEPGDVVMVKGSNGSKAGAIAAALSALDSRSGEGA

Sequences:

>Translated_465_residues
MAEPLWTAEEIAAATGGKLAGQAFRAGGVSIDTRTIQPGDLFVALAGVRDGHEFVEQAFEKGAAGVIASRQVFGPAVMVP
DTLHALEKLGAAARERAPQARRGAVTGSVGKTSVTQAIMAGLTLAGPAHSSVKSYNNHIGVPLTLARMPRGTARAVFEIG
MNHADEIRPLSRMVRPHAVVITTVGPVHTENFPDGEAGVARAKAEIFEGLEPGGVAVLNADNRWFDLLKGEAERAGATVR
TFGTGEACDARLIDFQVPAGHAVVQARLHGKALDFPILQTGLHWGLNSMAVLLMLEALGVDLDDGLAALGSFEPLAGRGA
EAQVRKGAGAFTLIDESYNANPISMASAIATLGARETAGRRIVALTDMLELGPEAEAFHRGLAEPLEAAAIDLVFCAGPL
MKSLWDALPPTRRGGYAESAAELAPQVVQAVEPGDVVMVKGSNGSKAGAIAAALSALDSRSGEGA
>Mature_464_residues
AEPLWTAEEIAAATGGKLAGQAFRAGGVSIDTRTIQPGDLFVALAGVRDGHEFVEQAFEKGAAGVIASRQVFGPAVMVPD
TLHALEKLGAAARERAPQARRGAVTGSVGKTSVTQAIMAGLTLAGPAHSSVKSYNNHIGVPLTLARMPRGTARAVFEIGM
NHADEIRPLSRMVRPHAVVITTVGPVHTENFPDGEAGVARAKAEIFEGLEPGGVAVLNADNRWFDLLKGEAERAGATVRT
FGTGEACDARLIDFQVPAGHAVVQARLHGKALDFPILQTGLHWGLNSMAVLLMLEALGVDLDDGLAALGSFEPLAGRGAE
AQVRKGAGAFTLIDESYNANPISMASAIATLGARETAGRRIVALTDMLELGPEAEAFHRGLAEPLEAAAIDLVFCAGPLM
KSLWDALPPTRRGGYAESAAELAPQVVQAVEPGDVVMVKGSNGSKAGAIAAALSALDSRSGEGA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=396, Percent_Identity=35.1010101010101, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48067; Mature: 47936

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00132 CARBOXYPEPT_ZN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPLWTAEEIAAATGGKLAGQAFRAGGVSIDTRTIQPGDLFVALAGVRDGHEFVEQAFE
CCCCCCCHHHHHHHCCCCHHHHHHHCCCCEEECEECCCCCEEEEEECCCCCHHHHHHHHH
KGAAGVIASRQVFGPAVMVPDTLHALEKLGAAARERAPQARRGAVTGSVGKTSVTQAIMA
CCCCCEEECHHHCCCEEECCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHH
GLTLAGPAHSSVKSYNNHIGVPLTLARMPRGTARAVFEIGMNHADEIRPLSRMVRPHAVV
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEE
ITTVGPVHTENFPDGEAGVARAKAEIFEGLEPGGVAVLNADNRWFDLLKGEAERAGATVR
EEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCHHHCCCEEE
TFGTGEACDARLIDFQVPAGHAVVQARLHGKALDFPILQTGLHWGLNSMAVLLMLEALGV
ECCCCCCCCCEEEEEECCCCHHEEEHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCC
DLDDGLAALGSFEPLAGRGAEAQVRKGAGAFTLIDESYNANPISMASAIATLGARETAGR
CCCCCHHHHCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHCCC
RIVALTDMLELGPEAEAFHRGLAEPLEAAAIDLVFCAGPLMKSLWDALPPTRRGGYAESA
EEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AELAPQVVQAVEPGDVVMVKGSNGSKAGAIAAALSALDSRSGEGA
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEPLWTAEEIAAATGGKLAGQAFRAGGVSIDTRTIQPGDLFVALAGVRDGHEFVEQAFE
CCCCCCHHHHHHHCCCCHHHHHHHCCCCEEECEECCCCCEEEEEECCCCCHHHHHHHHH
KGAAGVIASRQVFGPAVMVPDTLHALEKLGAAARERAPQARRGAVTGSVGKTSVTQAIMA
CCCCCEEECHHHCCCEEECCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHH
GLTLAGPAHSSVKSYNNHIGVPLTLARMPRGTARAVFEIGMNHADEIRPLSRMVRPHAVV
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEE
ITTVGPVHTENFPDGEAGVARAKAEIFEGLEPGGVAVLNADNRWFDLLKGEAERAGATVR
EEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCHHHCCCEEE
TFGTGEACDARLIDFQVPAGHAVVQARLHGKALDFPILQTGLHWGLNSMAVLLMLEALGV
ECCCCCCCCCEEEEEECCCCHHEEEHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCC
DLDDGLAALGSFEPLAGRGAEAQVRKGAGAFTLIDESYNANPISMASAIATLGARETAGR
CCCCCHHHHCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHCCC
RIVALTDMLELGPEAEAFHRGLAEPLEAAAIDLVFCAGPLMKSLWDALPPTRRGGYAESA
EEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AELAPQVVQAVEPGDVVMVKGSNGSKAGAIAAALSALDSRSGEGA
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]