Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 197105790

GI number: 197105790

Start: 2641071

End: 2642522

Strand: Reverse

Name: murE [H]

Synonym: PHZ_c2328

Alternate gene names: 197105790

Gene position: 2642522-2641071 (Counterclockwise)

Preceding gene: 197105791

Following gene: 197105789

Centisome position: 66.12

GC content: 75.0

Gene sequence:

>1452_bases
GTGAAGCGCCTCTCGGCCATCGTGAAGCGCGGCCTGCCGGCGGATCCCGAGATCACCGGCGTCACCGCCGACAGCCGCAA
GGTGCGGGCCGGCACGCTGTTCGCCGCCCTGCCGGGCACGAAGGTGGACGGCGCCAGCTTCGCCGCCGCCGCGATCCAGG
CCGGCGCCGCCGCGGTGATCGCCGACCGCGACCTCGGCCTGCCCGTCCCGACCATCGTGGCCAAGGACCCCCGCCGAGCC
TACGCCCTGGCCGCGGCCGAGTTCTGGGGACGCCAGCCGCAGGTCTGCGTCGCCGTCACCGGCACCAACGGCAAGACCTC
GGTGGCCAACTTCTGCCGCCAGATGTTCGCCCGCGCCGGCCGCACCTCGGCCAGCATGGGCACCCTGGGCGTCACCGTCT
CGCGGCCCGACGGGACGCACGAGCAGCTCACGCCCCCCGGCCTCACCACGCCCGACGCCGCCGACGTCGCCGAGATGCTG
GCGAGGATCGCCGGCATGGGCGTCAGCCACTTCGCGATGGAAGCCTCCTCGCACGGGGTGGACCAGCGCCGGCTGGACGG
GGTGAAGCTGACCGCCGCCGGCTTCCTGAACCTGACCCAGGACCACCTCGACTACCACGGCACGATGGAGGCCTACCGGG
CCGCCAAGCTTCGCCTGTTCGAGACCCTGCTGCCGCGCGGCGGCACGGCGGTGCTGAACGCCGACTCCGACGCCTTCCCG
GCCTTCGCCGCCGCCGCCGTGATCGCCGGCCAGTGGGTGATGCCGGTTGGCGAGACCGGCGCGACCCTGAAGCTGGCCAA
GCGCGAGCTGCTGCCCGAGGGCCAGTGGCTGCGGATCATGGCCGAGGGCCGCTCTCACGACGTGCGCCTGCCGCTGGCGG
GCGGGTTCCAGGCGTCGAACGCCCTGGTGGCCGCCGGCCTCTGCTGGGCCGCCGGCCTGACGCTGGAGGAGGTGTTCGCC
GGGCTGGAGGCGCTGGAGGGCGCGCCGGGACGGCTGCAGCGCGTGGGCCAGGGCGCCCGCGGCGGCGAGGCCTATGTGGA
CTACGCCCACACGCCCGACGGCCTGGAGACGGTGCTGAAGGCGCTGCGTCCCCACGTTCGCGGGCGGCTGATCGTGGTGT
TCGGGGCCGGCGGCGACCGCGACCGCGGCAAGCGGCCGCTGATGGGCAAGGTGGCGGGCGAGCTGGCCGACGTCGCCATC
GTCACCGACGACAACCCCCGCTCGGAGGTCCCGGCGGCGATCCGCGCCGAGGTGCTGGCGGGCTGCCCGGGCGCGGCCGA
GATCGGCGACCGCCGCGAGGCGATCCGCGAGGCCGTGGCGATGCTGCGCGACGGCGACATCCTGGTGGTCGCCGGCAAGG
GCCACGAGCAGGGCCAGATCGTGGGCGGCGTGACCCACCCCTTCGATGACGTGGCCGAGACGGCCCAGGCGCTGGAGCTG
GTCCATGGCTGA

Upstream 100 bases:

>100_bases
GCCGGGTCATCGAGCGCATCGCCCCGTTCCTGGGCGTCGAGCGCCAGCCCGAGCTCGCCGAGATCGCGCCCGCGGGCGCC
GCGCCGATGGGGGAGGGCCA

Downstream 100 bases:

>100_bases
GCCCCTCTGGACCGCCGAGGAGATCGCGGCCGCGACCGGCGGCAAGCTGGCGGGCCAGGCGTTCCGCGCGGGCGGCGTCT
CCATCGACACCCGCACGATC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKRLSAIVKRGLPADPEITGVTADSRKVRAGTLFAALPGTKVDGASFAAAAIQAGAAAVIADRDLGLPVPTIVAKDPRRA
YALAAAEFWGRQPQVCVAVTGTNGKTSVANFCRQMFARAGRTSASMGTLGVTVSRPDGTHEQLTPPGLTTPDAADVAEML
ARIAGMGVSHFAMEASSHGVDQRRLDGVKLTAAGFLNLTQDHLDYHGTMEAYRAAKLRLFETLLPRGGTAVLNADSDAFP
AFAAAAVIAGQWVMPVGETGATLKLAKRELLPEGQWLRIMAEGRSHDVRLPLAGGFQASNALVAAGLCWAAGLTLEEVFA
GLEALEGAPGRLQRVGQGARGGEAYVDYAHTPDGLETVLKALRPHVRGRLIVVFGAGGDRDRGKRPLMGKVAGELADVAI
VTDDNPRSEVPAAIRAEVLAGCPGAAEIGDRREAIREAVAMLRDGDILVVAGKGHEQGQIVGGVTHPFDDVAETAQALEL
VHG

Sequences:

>Translated_483_residues
MKRLSAIVKRGLPADPEITGVTADSRKVRAGTLFAALPGTKVDGASFAAAAIQAGAAAVIADRDLGLPVPTIVAKDPRRA
YALAAAEFWGRQPQVCVAVTGTNGKTSVANFCRQMFARAGRTSASMGTLGVTVSRPDGTHEQLTPPGLTTPDAADVAEML
ARIAGMGVSHFAMEASSHGVDQRRLDGVKLTAAGFLNLTQDHLDYHGTMEAYRAAKLRLFETLLPRGGTAVLNADSDAFP
AFAAAAVIAGQWVMPVGETGATLKLAKRELLPEGQWLRIMAEGRSHDVRLPLAGGFQASNALVAAGLCWAAGLTLEEVFA
GLEALEGAPGRLQRVGQGARGGEAYVDYAHTPDGLETVLKALRPHVRGRLIVVFGAGGDRDRGKRPLMGKVAGELADVAI
VTDDNPRSEVPAAIRAEVLAGCPGAAEIGDRREAIREAVAMLRDGDILVVAGKGHEQGQIVGGVTHPFDDVAETAQALEL
VHG
>Mature_483_residues
MKRLSAIVKRGLPADPEITGVTADSRKVRAGTLFAALPGTKVDGASFAAAAIQAGAAAVIADRDLGLPVPTIVAKDPRRA
YALAAAEFWGRQPQVCVAVTGTNGKTSVANFCRQMFARAGRTSASMGTLGVTVSRPDGTHEQLTPPGLTTPDAADVAEML
ARIAGMGVSHFAMEASSHGVDQRRLDGVKLTAAGFLNLTQDHLDYHGTMEAYRAAKLRLFETLLPRGGTAVLNADSDAFP
AFAAAAVIAGQWVMPVGETGATLKLAKRELLPEGQWLRIMAEGRSHDVRLPLAGGFQASNALVAAGLCWAAGLTLEEVFA
GLEALEGAPGRLQRVGQGARGGEAYVDYAHTPDGLETVLKALRPHVRGRLIVVFGAGGDRDRGKRPLMGKVAGELADVAI
VTDDNPRSEVPAAIRAEVLAGCPGAAEIGDRREAIREAVAMLRDGDILVVAGKGHEQGQIVGGVTHPFDDVAETAQALEL
VHG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=37.5, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786274, Length=321, Percent_Identity=30.5295950155763, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50291; Mature: 50291

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSAIVKRGLPADPEITGVTADSRKVRAGTLFAALPGTKVDGASFAAAAIQAGAAAVI
CHHHHHHHHCCCCCCCCEEEEECCCCEEECCEEEEECCCCCCCCCHHHHHHHHCCCEEEE
ADRDLGLPVPTIVAKDPRRAYALAAAEFWGRQPQVCVAVTGTNGKTSVANFCRQMFARAG
ECCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
RTSASMGTLGVTVSRPDGTHEQLTPPGLTTPDAADVAEMLARIAGMGVSHFAMEASSHGV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
DQRRLDGVKLTAAGFLNLTQDHLDYHGTMEAYRAAKLRLFETLLPRGGTAVLNADSDAFP
CHHHCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCH
AFAAAAVIAGQWVMPVGETGATLKLAKRELLPEGQWLRIMAEGRSHDVRLPLAGGFQASN
HHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCC
ALVAAGLCWAAGLTLEEVFAGLEALEGAPGRLQRVGQGARGGEAYVDYAHTPDGLETVLK
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
ALRPHVRGRLIVVFGAGGDRDRGKRPLMGKVAGELADVAIVTDDNPRSEVPAAIRAEVLA
HHCHHHCCEEEEEECCCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCHHCCHHHHHHHHH
GCPGAAEIGDRREAIREAVAMLRDGDILVVAGKGHEQGQIVGGVTHPFDDVAETAQALEL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHH
VHG
HCC
>Mature Secondary Structure
MKRLSAIVKRGLPADPEITGVTADSRKVRAGTLFAALPGTKVDGASFAAAAIQAGAAAVI
CHHHHHHHHCCCCCCCCEEEEECCCCEEECCEEEEECCCCCCCCCHHHHHHHHCCCEEEE
ADRDLGLPVPTIVAKDPRRAYALAAAEFWGRQPQVCVAVTGTNGKTSVANFCRQMFARAG
ECCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
RTSASMGTLGVTVSRPDGTHEQLTPPGLTTPDAADVAEMLARIAGMGVSHFAMEASSHGV
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
DQRRLDGVKLTAAGFLNLTQDHLDYHGTMEAYRAAKLRLFETLLPRGGTAVLNADSDAFP
CHHHCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCH
AFAAAAVIAGQWVMPVGETGATLKLAKRELLPEGQWLRIMAEGRSHDVRLPLAGGFQASN
HHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCC
ALVAAGLCWAAGLTLEEVFAGLEALEGAPGRLQRVGQGARGGEAYVDYAHTPDGLETVLK
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
ALRPHVRGRLIVVFGAGGDRDRGKRPLMGKVAGELADVAIVTDDNPRSEVPAAIRAEVLA
HHCHHHCCEEEEEECCCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCHHCCHHHHHHHHH
GCPGAAEIGDRREAIREAVAMLRDGDILVVAGKGHEQGQIVGGVTHPFDDVAETAQALEL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHH
VHG
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]