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The map label for this gene is acoA [H]

Identifier: 197105588

GI number: 197105588

Start: 2393179

End: 2394162

Strand: Direct

Name: acoA [H]

Synonym: PHZ_c2125

Alternate gene names: 197105588

Gene position: 2393179-2394162 (Clockwise)

Preceding gene: 197105587

Following gene: 197105589

Centisome position: 59.89

GC content: 70.02

Gene sequence:

>984_bases
ATGGCGCTCGAGGCGGCCCAGCAGCTGGACCTGTTCGCTCGGATGCTCCGCATCCGACGGTTCGAGGAAGCGCTCATCGC
GCATCGGCCGGTGGGGCACCTCAGCATCGGACAGGAAGCGGCCATTGCGGGCGCCTGCCTGGCGCTGCGCGACGACGACT
ACGTCACCGGCACCCACCGCTCCCACGGCCATCCGATCGGCAAGGGCGCCGAGATCGCCCCGCTGATGGCCGAGATCTTC
GGCAAGCGCACCGGCATCTGCCGGGGGCTCGGCGGGTCCATGCACCTGACCGACACCAGCCGCGGCCTGATCGGCGAATC
CGCCATCGTCGGCGGCGGCCTGCCGCTGGCCACCGGCGCGGGCCTGAGCATCCAGGTCCGCAAGACCGACCAGGTGAGCC
TCTGCTTCTTCGGCGACGGCGCGGCCAACCAGGGCACCTTCGGCGAGAGCCTGAACATGGCCGCCGTCTGGAAGCTGCCG
GTCATCTACTTCTGCGAGAACAACGGCTACGCGGTCACGACGCCCGTCGAGAAGTCGCACGGCCAGCCCGACATCGCCCG
CCGGGCGAACGGCTACGGCATGCCCGGCGTGATCGTGGACGGCCAGGACGCCGAGGCGGTCTACGACGTGACCCGCGCGG
CCGTCACGAGGGCGCGTTCGGGCGCCGGCCCCACCCTCATCGAGGCCAAGACCTATCGCTTCGACGAACACTCCAACCGC
CTGGCGATCCCCATCCGCTATCGCAGCGAGGAGGAGATCGAGCACCACCGGACCCAGCGCGACCCCATCACGCTCTATCG
CGCGGTGCTGGTCTCGCGCGGGCTGCAGGCCGACGTGGAGGCGATCGAGGCCGAGGTCGCCGAGACCATGGCCGCGGCCG
TCGAGTTCGCCAAGGAGAGCCCCGAGCCCGAACTGCAGGACCTGCTGGACTGCATGTACAGCCTTCCCATCCATTTTCCG
CCGCGCGGCGAAGGGGTCTTCTGA

Upstream 100 bases:

>100_bases
ATCGAGGCGCCGGTGGCCGGCGCGCTGGCGAGCATCCGCTTCCAGGAGGGCGACGAGGTCGAGGTCGAGGCCGAGATCGC
CACGATCGAGGAAGGCTGAC

Downstream 100 bases:

>100_bases
CGTGCGCAACTCGACCCAGAAGCTCACCTACTTGCAGGCGATCATCGAGGCCCAGCGCGAGGAGATGCGGCGCGACGAGC
GCGTGATCCTCATGGGCGAG

Product: thiamine pyrophosphate-dependent dehydrogenase, E1 component alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MALEAAQQLDLFARMLRIRRFEEALIAHRPVGHLSIGQEAAIAGACLALRDDDYVTGTHRSHGHPIGKGAEIAPLMAEIF
GKRTGICRGLGGSMHLTDTSRGLIGESAIVGGGLPLATGAGLSIQVRKTDQVSLCFFGDGAANQGTFGESLNMAAVWKLP
VIYFCENNGYAVTTPVEKSHGQPDIARRANGYGMPGVIVDGQDAEAVYDVTRAAVTRARSGAGPTLIEAKTYRFDEHSNR
LAIPIRYRSEEEIEHHRTQRDPITLYRAVLVSRGLQADVEAIEAEVAETMAAAVEFAKESPEPELQDLLDCMYSLPIHFP
PRGEGVF

Sequences:

>Translated_327_residues
MALEAAQQLDLFARMLRIRRFEEALIAHRPVGHLSIGQEAAIAGACLALRDDDYVTGTHRSHGHPIGKGAEIAPLMAEIF
GKRTGICRGLGGSMHLTDTSRGLIGESAIVGGGLPLATGAGLSIQVRKTDQVSLCFFGDGAANQGTFGESLNMAAVWKLP
VIYFCENNGYAVTTPVEKSHGQPDIARRANGYGMPGVIVDGQDAEAVYDVTRAAVTRARSGAGPTLIEAKTYRFDEHSNR
LAIPIRYRSEEEIEHHRTQRDPITLYRAVLVSRGLQADVEAIEAEVAETMAAAVEFAKESPEPELQDLLDCMYSLPIHFP
PRGEGVF
>Mature_326_residues
ALEAAQQLDLFARMLRIRRFEEALIAHRPVGHLSIGQEAAIAGACLALRDDDYVTGTHRSHGHPIGKGAEIAPLMAEIFG
KRTGICRGLGGSMHLTDTSRGLIGESAIVGGGLPLATGAGLSIQVRKTDQVSLCFFGDGAANQGTFGESLNMAAVWKLPV
IYFCENNGYAVTTPVEKSHGQPDIARRANGYGMPGVIVDGQDAEAVYDVTRAAVTRARSGAGPTLIEAKTYRFDEHSNRL
AIPIRYRSEEEIEHHRTQRDPITLYRAVLVSRGLQADVEAIEAEVAETMAAAVEFAKESPEPELQDLLDCMYSLPIHFPP
RGEGVF

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=330, Percent_Identity=37.2727272727273, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4505685, Length=324, Percent_Identity=36.7283950617284, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI291084742, Length=324, Percent_Identity=36.7283950617284, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI291084744, Length=293, Percent_Identity=37.8839590443686, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI11386135, Length=312, Percent_Identity=30.4487179487179, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI291084757, Length=324, Percent_Identity=32.7160493827161, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI258645172, Length=312, Percent_Identity=30.4487179487179, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI32564172, Length=306, Percent_Identity=35.6209150326797, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17536047, Length=306, Percent_Identity=35.6209150326797, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI86563357, Length=318, Percent_Identity=28.9308176100629, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI86563355, Length=318, Percent_Identity=28.9308176100629, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=308, Percent_Identity=36.6883116883117, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24639744, Length=334, Percent_Identity=36.2275449101796, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI28571106, Length=334, Percent_Identity=36.2275449101796, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24639740, Length=334, Percent_Identity=36.2275449101796, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI24639748, Length=328, Percent_Identity=36.5853658536585, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24639746, Length=320, Percent_Identity=36.875, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21355903, Length=321, Percent_Identity=27.1028037383178, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35308; Mature: 35176

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEAAQQLDLFARMLRIRRFEEALIAHRPVGHLSIGQEAAIAGACLALRDDDYVTGTHR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCEEECCCC
SHGHPIGKGAEIAPLMAEIFGKRTGICRGLGGSMHLTDTSRGLIGESAIVGGGLPLATGA
CCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCEEECCCCCCCCCCCEEECCCCCCCCCC
GLSIQVRKTDQVSLCFFGDGAANQGTFGESLNMAAVWKLPVIYFCENNGYAVTTPVEKSH
CCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCEEEEECCCCEEEECCHHHCC
GQPDIARRANGYGMPGVIVDGQDAEAVYDVTRAAVTRARSGAGPTLIEAKTYRFDEHSNR
CCCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCE
LAIPIRYRSEEEIEHHRTQRDPITLYRAVLVSRGLQADVEAIEAEVAETMAAAVEFAKES
EEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PEPELQDLLDCMYSLPIHFPPRGEGVF
CCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ALEAAQQLDLFARMLRIRRFEEALIAHRPVGHLSIGQEAAIAGACLALRDDDYVTGTHR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCEEECCCC
SHGHPIGKGAEIAPLMAEIFGKRTGICRGLGGSMHLTDTSRGLIGESAIVGGGLPLATGA
CCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCEEECCCCCCCCCCCEEECCCCCCCCCC
GLSIQVRKTDQVSLCFFGDGAANQGTFGESLNMAAVWKLPVIYFCENNGYAVTTPVEKSH
CCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCEEEEECCCCEEEECCHHHCC
GQPDIARRANGYGMPGVIVDGQDAEAVYDVTRAAVTRARSGAGPTLIEAKTYRFDEHSNR
CCCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCE
LAIPIRYRSEEEIEHHRTQRDPITLYRAVLVSRGLQADVEAIEAEVAETMAAAVEFAKES
EEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PEPELQDLLDCMYSLPIHFPPRGEGVF
CCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]