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The map label for this gene is glnG [H]

Identifier: 197105193

GI number: 197105193

Start: 1937972

End: 1939393

Strand: Reverse

Name: glnG [H]

Synonym: PHZ_c1730

Alternate gene names: 197105193

Gene position: 1939393-1937972 (Counterclockwise)

Preceding gene: 197105194

Following gene: 197105192

Centisome position: 48.53

GC content: 70.32

Gene sequence:

>1422_bases
ATGAACGCCGCGTCGAAGAAGATCCTGATCGCCGACGACGATTCCTCGATCCGGCTCGTGCTCAGCCAGGCGTTCACGCG
GCTGGGCTACCAGGTCCGGGCCACCGGCAACGCCACCACGCTCCTGAAGTGGGTTTCCGACGGGGAGGGCGACCTCGTCG
TCACCGACGTCGTGATGCCTGACGAGAACGTCTTCGATGTCCTGCCGCGCATCCGCAAGCAGCGGCCGAAGCTGCCCGTG
ATCGTGATGAGCGCCCAGAACAACCTGATCACTGCCGTGAACGCAGCCGAGGTCGGCGCCTTCGAATATGTGCCCAAGCC
CTTCGATCTCGACGACATGACCTCGGCCGCCCGCCGGGCCCTGTCGCGCCCGGCCGATTCCGAGGCCTCCAAGGCCCAGG
CGCGGGCCGTGCGCGACGACCGCCTGCCGCTGATCGGCCGCTCGGCGCCGATGCAGGAGGTCTACCGGACCATCGCCCGG
CTCGTCGGCGCGGATCTGACGGTGCTGATCACCGGCGAGAGCGGCACCGGCAAGGAACTGGTCGCCCGCGCCCTGCACGA
CATGGGCCGCCGCCGCGACGGCAAGTTCGTGGTCATCAACCTGGCCGCCACGCCCCGCGAGCGCGTCGAGGCCGAGCTGT
TCGGCAAGGAGGACGGCGACCACGGCAAGCTGGTCGAGGCCGACGGCGGGACGCTGTTCCTGGACGAGATCGGCGACATG
CCGCTCGACGCCCAGACCCGCCTCCTGCGGGTGATCGACGGCTCGGAGCCGACGGTGAATCCCAAGACCGGCCGCCGGCC
GAACATGCGGATCATCGCCGCCACCAACCGCGACCTGCGGGCGCTGATCCAGCAGGGTCTGTTCCGCGAGGACCTGTTCT
TCCGCCTGAACGTCGCCCCGCTGCGCCTGCCGCCCCTTCGCGACCGGGTCGAGGACATCCCGGACCTGGCGCGGGCCTTC
CTGCTGCGCGCCAACCGCGAGGGCCTGCCCGCCAAGACCATCGATTCGGGCGCCCTGGAGCGCCTCAAGCGCCATCCCTG
GCCGGGGAACGTGCGCGAGCTCGAGAACCTGGTGCGCCGGATCTGCGCCCTTTACGGCGAGGACCTGATCTCGGCGCGGA
TCATCGAGCGCGAGCTGGCCGACCAGCAGCCGGTCGTCCAGGGGCAGGAGGGGCCGGCCACCCTGGCCCAGCTCGTCGAG
CAGAAGCTCTCGACCTATTTCGCCGACCAGCCGGACGGCATTCCCCCGCCGGGCCTCTACGATCGCGTGCTGGAGGAGGT
GGAGCGGCCGCTGATCCAGCTCACGCTCGCCGCCACGCGGGGCAACCAGGTCCGGGCGGCGGAGATCCTGGGCCTCAACC
GCAACACCCTGCGCAAGAAGATCCAGGACCTGGGCGTGGAGATGAGCCGCGGCCGTCGCTGA

Upstream 100 bases:

>100_bases
TGGTCGCCGGCCACGGCGGCCTGATCGATTTCGAATCCGAGCCGGGCCGCACCGTCTTCCGGGTGCTGCTCCCCATCGCC
GCGCCCGAGGACCTGCCCGC

Downstream 100 bases:

>100_bases
CGGCTGTGTGAAGCATCGGCGCAACATAGCTGTTGAGTTCGGAGCACAGTCCCTTAGGCTCCCGACGCAATGGCGTTGCC
TATCAACCCCGCGTTCGCGC

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVSDGEGDLVVTDVVMPDENVFDVLPRIRKQRPKLPV
IVMSAQNNLITAVNAAEVGAFEYVPKPFDLDDMTSAARRALSRPADSEASKAQARAVRDDRLPLIGRSAPMQEVYRTIAR
LVGADLTVLITGESGTGKELVARALHDMGRRRDGKFVVINLAATPRERVEAELFGKEDGDHGKLVEADGGTLFLDEIGDM
PLDAQTRLLRVIDGSEPTVNPKTGRRPNMRIIAATNRDLRALIQQGLFREDLFFRLNVAPLRLPPLRDRVEDIPDLARAF
LLRANREGLPAKTIDSGALERLKRHPWPGNVRELENLVRRICALYGEDLISARIIERELADQQPVVQGQEGPATLAQLVE
QKLSTYFADQPDGIPPPGLYDRVLEEVERPLIQLTLAATRGNQVRAAEILGLNRNTLRKKIQDLGVEMSRGRR

Sequences:

>Translated_473_residues
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVSDGEGDLVVTDVVMPDENVFDVLPRIRKQRPKLPV
IVMSAQNNLITAVNAAEVGAFEYVPKPFDLDDMTSAARRALSRPADSEASKAQARAVRDDRLPLIGRSAPMQEVYRTIAR
LVGADLTVLITGESGTGKELVARALHDMGRRRDGKFVVINLAATPRERVEAELFGKEDGDHGKLVEADGGTLFLDEIGDM
PLDAQTRLLRVIDGSEPTVNPKTGRRPNMRIIAATNRDLRALIQQGLFREDLFFRLNVAPLRLPPLRDRVEDIPDLARAF
LLRANREGLPAKTIDSGALERLKRHPWPGNVRELENLVRRICALYGEDLISARIIERELADQQPVVQGQEGPATLAQLVE
QKLSTYFADQPDGIPPPGLYDRVLEEVERPLIQLTLAATRGNQVRAAEILGLNRNTLRKKIQDLGVEMSRGRR
>Mature_473_residues
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVSDGEGDLVVTDVVMPDENVFDVLPRIRKQRPKLPV
IVMSAQNNLITAVNAAEVGAFEYVPKPFDLDDMTSAARRALSRPADSEASKAQARAVRDDRLPLIGRSAPMQEVYRTIAR
LVGADLTVLITGESGTGKELVARALHDMGRRRDGKFVVINLAATPRERVEAELFGKEDGDHGKLVEADGGTLFLDEIGDM
PLDAQTRLLRVIDGSEPTVNPKTGRRPNMRIIAATNRDLRALIQQGLFREDLFFRLNVAPLRLPPLRDRVEDIPDLARAF
LLRANREGLPAKTIDSGALERLKRHPWPGNVRELENLVRRICALYGEDLISARIIERELADQQPVVQGQEGPATLAQLVE
QKLSTYFADQPDGIPPPGLYDRVLEEVERPLIQLTLAATRGNQVRAAEILGLNRNTLRKKIQDLGVEMSRGRR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=477, Percent_Identity=40.461215932914, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788550, Length=476, Percent_Identity=36.5546218487395, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790437, Length=464, Percent_Identity=37.7155172413793, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788905, Length=472, Percent_Identity=34.1101694915254, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=38.2530120481928, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082117, Length=229, Percent_Identity=48.0349344978166, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=38.7573964497041, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=43.7229437229437, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081872, Length=334, Percent_Identity=35.6287425149701, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1786524, Length=313, Percent_Identity=39.6166134185303, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787583, Length=220, Percent_Identity=39.5454545454545, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=33.6206896551724, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=28.0254777070064, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=31.6666666666667, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790552, Length=125, Percent_Identity=32, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789661, Length=68, Percent_Identity=50, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=26.890756302521, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52433; Mature: 52433

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVSDGEGDLVVTDVVMP
CCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCEEEEEEECC
DENVFDVLPRIRKQRPKLPVIVMSAQNNLITAVNAAEVGAFEYVPKPFDLDDMTSAARRA
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHCCCHHCCCCCCCHHHHHHHHHHH
LSRPADSEASKAQARAVRDDRLPLIGRSAPMQEVYRTIARLVGADLTVLITGESGTGKEL
HCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
VARALHDMGRRRDGKFVVINLAATPRERVEAELFGKEDGDHGKLVEADGGTLFLDEIGDM
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCEEEHHHHCCC
PLDAQTRLLRVIDGSEPTVNPKTGRRPNMRIIAATNRDLRALIQQGLFREDLFFRLNVAP
CCCHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCHHCCEEEEECCC
LRLPPLRDRVEDIPDLARAFLLRANREGLPAKTIDSGALERLKRHPWPGNVRELENLVRR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
ICALYGEDLISARIIERELADQQPVVQGQEGPATLAQLVEQKLSTYFADQPDGIPPPGLY
HHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
DRVLEEVERPLIQLTLAATRGNQVRAAEILGLNRNTLRKKIQDLGVEMSRGRR
HHHHHHHHCCCEEEEEEECCCCCEEEHHHCCCCHHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MNAASKKILIADDDSSIRLVLSQAFTRLGYQVRATGNATTLLKWVSDGEGDLVVTDVVMP
CCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCEEEEEEECC
DENVFDVLPRIRKQRPKLPVIVMSAQNNLITAVNAAEVGAFEYVPKPFDLDDMTSAARRA
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECHHHCCCHHCCCCCCCHHHHHHHHHHH
LSRPADSEASKAQARAVRDDRLPLIGRSAPMQEVYRTIARLVGADLTVLITGESGTGKEL
HCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
VARALHDMGRRRDGKFVVINLAATPRERVEAELFGKEDGDHGKLVEADGGTLFLDEIGDM
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCEEEHHHHCCC
PLDAQTRLLRVIDGSEPTVNPKTGRRPNMRIIAATNRDLRALIQQGLFREDLFFRLNVAP
CCCHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCHHCCEEEEECCC
LRLPPLRDRVEDIPDLARAFLLRANREGLPAKTIDSGALERLKRHPWPGNVRELENLVRR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
ICALYGEDLISARIIERELADQQPVVQGQEGPATLAQLVEQKLSTYFADQPDGIPPPGLY
HHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
DRVLEEVERPLIQLTLAATRGNQVRAAEILGLNRNTLRKKIQDLGVEMSRGRR
HHHHHHHHCCCEEEEEEECCCCCEEEHHHCCCCHHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]