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The map label for this gene is tgt [H]

Identifier: 197104870

GI number: 197104870

Start: 1587029

End: 1588144

Strand: Direct

Name: tgt [H]

Synonym: PHZ_c1404

Alternate gene names: 197104870

Gene position: 1587029-1588144 (Clockwise)

Preceding gene: 197104869

Following gene: 197104873

Centisome position: 39.71

GC content: 71.59

Gene sequence:

>1116_bases
ATGGCCGCGTTCCCGTTCACCATCGCCGCCACGGACGGCAAGGCGCGCACCGGCGTGCTCTCCACGCCTCGCGGCGATAT
CCGGACGCCGGCGTTCATGCCGGTGGGCACGGCGGGGACCGTCAAGGCGCTGACGGTGGAGCAGGTGGCCGCGACGGGCG
CCGACATCCTGCTGGGCAACACCTATCACCTCATGCTGCGCCCGACGGCCGAGCGGGTCCGCAGGCTGGGCGGCCTGCAC
AGGTTCATGCGCTGGGAGAAGCCGATCCTGACGGACAGCGGCGGCTTCCAGGTCATGTCGCTGTCGCAGATCTCGAAGGT
CACCGAGGACAGCGTCACCTTCCAGAGCCACCTGGACGGCAGCCGGCACGTGCTGACGCCCGAGCGCTCGATCGAGATCC
AGGCCGACCTGCTGGGCTCCGACATCGTCATGCAGCTGGACGAGCTGGTCGCCCTGCCGGCCGAGCGCGACCGGGCGGCC
GAGGCCATGCGCCGGTCCGCGCGCTGGGGACAGCGGTGCAGGGACGCGTTCGGCGCGCGCGAGGCCCAGGCGCTGTTCGC
CATCCAGCAGGGCGGCACGGACGAGGCGCTGCGCGCCGAGTCGGCCGAGCGGCTGCGCGAGATCGGCTTCGACGGCTACG
CCATCGGCGGCCTGGCGGTGGGCGAGGGCCACGAGGCCATGTGCGAGGTGCTGGACTATGCGCCCGGCATGCTTCCCGCC
GAGCGGCCGCGCTATCTGATGGGCGTCGGCAAGCCGATCGACATCGTCGAGGCGGTCTACCGCGGGGTGGACATGTTCGA
CTGCGTCCTGCCCACCCGCTCGGGCCGCCACGGCCAGGCGTGGACCTGGGACGGGCCGCTGAACCTGAAGAACGCCCGCT
TCGCCGAGGACGAGGAGCCGCTCGACCCCACCATCCCCGTCTCGGCCAGCCGCGACTATTCGAAGGCCTATCTGCACCAC
CTGGTGAAGGCCGGCGAGATCCTGGGCCAGGTGCTGCTGTCCTGGCACAACGTGGCCTTCTTCCAGGCGCTGATGGGCGA
GATGCGCGCGGCCATCGCCGAGAGGCGCATGGAGGCCTTCCGCCGCGACTTCGCCGCCCGCCAGAAGGCGCGCTGA

Upstream 100 bases:

>100_bases
TCGCGGGGACCGACACCATGCGGGCGGCCTACCGCCACGCCATCGAGACCGGCTACCGCTTCTATTCCTACGGCGACAGC
TCGCTGCTCTGGAGAGCCTG

Downstream 100 bases:

>100_bases
GCCCCGCTAGGGCCGTCCCTGCGGGAGGTCCTCGGGACGCGTCGGATAGCGCGGCTGAAACCGCCGGTCCGGATCGGGCG
GCGGCGTGCCGGCCTTCTCG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MAAFPFTIAATDGKARTGVLSTPRGDIRTPAFMPVGTAGTVKALTVEQVAATGADILLGNTYHLMLRPTAERVRRLGGLH
RFMRWEKPILTDSGGFQVMSLSQISKVTEDSVTFQSHLDGSRHVLTPERSIEIQADLLGSDIVMQLDELVALPAERDRAA
EAMRRSARWGQRCRDAFGAREAQALFAIQQGGTDEALRAESAERLREIGFDGYAIGGLAVGEGHEAMCEVLDYAPGMLPA
ERPRYLMGVGKPIDIVEAVYRGVDMFDCVLPTRSGRHGQAWTWDGPLNLKNARFAEDEEPLDPTIPVSASRDYSKAYLHH
LVKAGEILGQVLLSWHNVAFFQALMGEMRAAIAERRMEAFRRDFAARQKAR

Sequences:

>Translated_371_residues
MAAFPFTIAATDGKARTGVLSTPRGDIRTPAFMPVGTAGTVKALTVEQVAATGADILLGNTYHLMLRPTAERVRRLGGLH
RFMRWEKPILTDSGGFQVMSLSQISKVTEDSVTFQSHLDGSRHVLTPERSIEIQADLLGSDIVMQLDELVALPAERDRAA
EAMRRSARWGQRCRDAFGAREAQALFAIQQGGTDEALRAESAERLREIGFDGYAIGGLAVGEGHEAMCEVLDYAPGMLPA
ERPRYLMGVGKPIDIVEAVYRGVDMFDCVLPTRSGRHGQAWTWDGPLNLKNARFAEDEEPLDPTIPVSASRDYSKAYLHH
LVKAGEILGQVLLSWHNVAFFQALMGEMRAAIAERRMEAFRRDFAARQKAR
>Mature_370_residues
AAFPFTIAATDGKARTGVLSTPRGDIRTPAFMPVGTAGTVKALTVEQVAATGADILLGNTYHLMLRPTAERVRRLGGLHR
FMRWEKPILTDSGGFQVMSLSQISKVTEDSVTFQSHLDGSRHVLTPERSIEIQADLLGSDIVMQLDELVALPAERDRAAE
AMRRSARWGQRCRDAFGAREAQALFAIQQGGTDEALRAESAERLREIGFDGYAIGGLAVGEGHEAMCEVLDYAPGMLPAE
RPRYLMGVGKPIDIVEAVYRGVDMFDCVLPTRSGRHGQAWTWDGPLNLKNARFAEDEEPLDPTIPVSASRDYSKAYLHHL
VKAGEILGQVLLSWHNVAFFQALMGEMRAAIAERRMEAFRRDFAARQKAR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=351, Percent_Identity=45.2991452991453, Blast_Score=305, Evalue=5e-83,
Organism=Escherichia coli, GI1786607, Length=364, Percent_Identity=51.6483516483517, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=43.6111111111111, Blast_Score=284, Evalue=4e-77,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=45.9154929577465, Blast_Score=314, Evalue=5e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 40880; Mature: 40749

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFPFTIAATDGKARTGVLSTPRGDIRTPAFMPVGTAGTVKALTVEQVAATGADILLGN
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECC
TYHLMLRPTAERVRRLGGLHRFMRWEKPILTDSGGFQVMSLSQISKVTEDSVTFQSHLDG
EEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHHCCC
SRHVLTPERSIEIQADLLGSDIVMQLDELVALPAERDRAAEAMRRSARWGQRCRDAFGAR
CCEEECCCCCEEEEHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EAQALFAIQQGGTDEALRAESAERLREIGFDGYAIGGLAVGEGHEAMCEVLDYAPGMLPA
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCC
ERPRYLMGVGKPIDIVEAVYRGVDMFDCVLPTRSGRHGQAWTWDGPLNLKNARFAEDEEP
CCCCCEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCC
LDPTIPVSASRDYSKAYLHHLVKAGEILGQVLLSWHNVAFFQALMGEMRAAIAERRMEAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDFAARQKAR
HHHHHHHHCCC
>Mature Secondary Structure 
AAFPFTIAATDGKARTGVLSTPRGDIRTPAFMPVGTAGTVKALTVEQVAATGADILLGN
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECC
TYHLMLRPTAERVRRLGGLHRFMRWEKPILTDSGGFQVMSLSQISKVTEDSVTFQSHLDG
EEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHHHHHHHCCC
SRHVLTPERSIEIQADLLGSDIVMQLDELVALPAERDRAAEAMRRSARWGQRCRDAFGAR
CCEEECCCCCEEEEHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
EAQALFAIQQGGTDEALRAESAERLREIGFDGYAIGGLAVGEGHEAMCEVLDYAPGMLPA
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCC
ERPRYLMGVGKPIDIVEAVYRGVDMFDCVLPTRSGRHGQAWTWDGPLNLKNARFAEDEEP
CCCCCEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCC
LDPTIPVSASRDYSKAYLHHLVKAGEILGQVLLSWHNVAFFQALMGEMRAAIAERRMEAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRDFAARQKAR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]