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The map label for this gene is queA

Identifier: 197104869

GI number: 197104869

Start: 1585956

End: 1587029

Strand: Direct

Name: queA

Synonym: PHZ_c1403

Alternate gene names: 197104869

Gene position: 1585956-1587029 (Clockwise)

Preceding gene: 197104867

Following gene: 197104870

Centisome position: 39.69

GC content: 73.09

Gene sequence:

>1074_bases
ATGAAACTCGCCGACTTCGACTTCGACCTGCCCGAGGAGGCCATCGCCCTCCGGCCCGCGAACCCGCGCGACGCCGCGCG
GCTGCTGCTCGTCGAGCCGGGGCAGGACTTCCGCGACCTCGCGGTGCGCGACCTGCCGGGGCTGCTGCGGGCCGGCGACG
TGCTGGTGCTGAACGACACCCGCGTCATCCCGGCCCGCCTGAAGGGCGTCCGCACCCGCGAGGGGAGCCGCGTGGCCGTG
GAGGCGACCCTGCACCAGCGCAAGGCGGGCCACGTCTGGACCGCCTTCATGCGCCCCGGCAAACGCCTGGCGCCCGGCGA
CCGGGTCAGCTTCGGCGAGACCGACGACCGGGCGTGCTTCCTCGGCGCGCTCGACGCCACGGTGAAGGAGAAGGGCGAGG
GCGGCGAGGTGACCTTGGCCTTCGACCTTTCGGGCCCGGACCTCGACGCCGCGATCGCCGAGCGCGGGGCCATGCCGCTG
CCCCCCTACATCGCCGCCAAGCGGGCCGAGGACGAGCAGGACCGCGCCGACTACCAGACCGTCTACGCCGAGGAGGACGG
CTCGGTGGCCGCGCCGACCGCGGGGCTGCACTTCACGCCCGAGCTGCTGGCGAGGCTGGCGCAGGCCGGGGTCACGACCG
AGCGGGTCACCCTGCATGTCGGCGCCGGCACCTTTCTGCCGGTCAAGACCGAGGACATCTCCGAGCACCGGATGCACGCC
GAGTGGGGGGAGGTGGACGCCGCGACGGCCGATCGCCTGAACGCCGCCCGCGCGGCCGGCGGCCGCATCGTCTGCGTCGG
CACCACCTCGCTGCGGCTGCTGGAGTCGGCCGCCGGCGAGGACGGCGTGGTCCGCCCGTTCCGGGACGAAACCGCGATCT
TCATCACGCCGGGCTACCGTTTCCGCGCCGCCGACGGGCTGATGACCAACTTCCACCTGCCGAAGTCGACGCTGTTCATG
CTGGTCTGCGCCTTCGCGGGGACCGACACCATGCGGGCGGCCTACCGCCACGCCATCGAGACCGGCTACCGCTTCTATTC
CTACGGCGACAGCTCGCTGCTCTGGAGAGCCTGA

Upstream 100 bases:

>100_bases
GAGCACCGTGAAGGCGACTCGCAGGGCGTGCATCGCCTTCGCCTAGCATGGCGCGAGCCTTGACGCGCCTGCGGCCCCGT
GGCCTTCACGCGGACCCGCC

Downstream 100 bases:

>100_bases
TGGCCGCGTTCCCGTTCACCATCGCCGCCACGGACGGCAAGGCGCGCACCGGCGTGCTCTCCACGCCTCGCGGCGATATC
CGGACGCCGGCGTTCATGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKLADFDFDLPEEAIALRPANPRDAARLLLVEPGQDFRDLAVRDLPGLLRAGDVLVLNDTRVIPARLKGVRTREGSRVAV
EATLHQRKAGHVWTAFMRPGKRLAPGDRVSFGETDDRACFLGALDATVKEKGEGGEVTLAFDLSGPDLDAAIAERGAMPL
PPYIAAKRAEDEQDRADYQTVYAEEDGSVAAPTAGLHFTPELLARLAQAGVTTERVTLHVGAGTFLPVKTEDISEHRMHA
EWGEVDAATADRLNAARAAGGRIVCVGTTSLRLLESAAGEDGVVRPFRDETAIFITPGYRFRAADGLMTNFHLPKSTLFM
LVCAFAGTDTMRAAYRHAIETGYRFYSYGDSSLLWRA

Sequences:

>Translated_357_residues
MKLADFDFDLPEEAIALRPANPRDAARLLLVEPGQDFRDLAVRDLPGLLRAGDVLVLNDTRVIPARLKGVRTREGSRVAV
EATLHQRKAGHVWTAFMRPGKRLAPGDRVSFGETDDRACFLGALDATVKEKGEGGEVTLAFDLSGPDLDAAIAERGAMPL
PPYIAAKRAEDEQDRADYQTVYAEEDGSVAAPTAGLHFTPELLARLAQAGVTTERVTLHVGAGTFLPVKTEDISEHRMHA
EWGEVDAATADRLNAARAAGGRIVCVGTTSLRLLESAAGEDGVVRPFRDETAIFITPGYRFRAADGLMTNFHLPKSTLFM
LVCAFAGTDTMRAAYRHAIETGYRFYSYGDSSLLWRA
>Mature_357_residues
MKLADFDFDLPEEAIALRPANPRDAARLLLVEPGQDFRDLAVRDLPGLLRAGDVLVLNDTRVIPARLKGVRTREGSRVAV
EATLHQRKAGHVWTAFMRPGKRLAPGDRVSFGETDDRACFLGALDATVKEKGEGGEVTLAFDLSGPDLDAAIAERGAMPL
PPYIAAKRAEDEQDRADYQTVYAEEDGSVAAPTAGLHFTPELLARLAQAGVTTERVTLHVGAGTFLPVKTEDISEHRMHA
EWGEVDAATADRLNAARAAGGRIVCVGTTSLRLLESAAGEDGVVRPFRDETAIFITPGYRFRAADGLMTNFHLPKSTLFM
LVCAFAGTDTMRAAYRHAIETGYRFYSYGDSSLLWRA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=359, Percent_Identity=44.0111420612813, Blast_Score=260, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PHEZH (B4R9Q3)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130246.1
- GeneID:   6790909
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1403
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38798; Mature: 38798

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLADFDFDLPEEAIALRPANPRDAARLLLVEPGQDFRDLAVRDLPGLLRAGDVLVLNDT
CCCCCCCCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHCCCEEEEECC
RVIPARLKGVRTREGSRVAVEATLHQRKAGHVWTAFMRPGKRLAPGDRVSFGETDDRACF
EECHHHHCCCCCCCCCEEEEEEEHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
LGALDATVKEKGEGGEVTLAFDLSGPDLDAAIAERGAMPLPPYIAAKRAEDEQDRADYQT
EEEHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCHHHHCCCCCHHHHCCEEE
VYAEEDGSVAAPTAGLHFTPELLARLAQAGVTTERVTLHVGAGTFLPVKTEDISEHRMHA
EEECCCCCEECCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCHHHHHHC
EWGEVDAATADRLNAARAAGGRIVCVGTTSLRLLESAAGEDGVVRPFRDETAIFITPGYR
CCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCE
FRAADGLMTNFHLPKSTLFMLVCAFAGTDTMRAAYRHAIETGYRFYSYGDSSLLWRA
EEECCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEEEC
>Mature Secondary Structure
MKLADFDFDLPEEAIALRPANPRDAARLLLVEPGQDFRDLAVRDLPGLLRAGDVLVLNDT
CCCCCCCCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHCCCEEEEECC
RVIPARLKGVRTREGSRVAVEATLHQRKAGHVWTAFMRPGKRLAPGDRVSFGETDDRACF
EECHHHHCCCCCCCCCEEEEEEEHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
LGALDATVKEKGEGGEVTLAFDLSGPDLDAAIAERGAMPLPPYIAAKRAEDEQDRADYQT
EEEHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCHHHHCCCCCHHHHCCEEE
VYAEEDGSVAAPTAGLHFTPELLARLAQAGVTTERVTLHVGAGTFLPVKTEDISEHRMHA
EEECCCCCEECCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCHHHHHHC
EWGEVDAATADRLNAARAAGGRIVCVGTTSLRLLESAAGEDGVVRPFRDETAIFITPGYR
CCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCE
FRAADGLMTNFHLPKSTLFMLVCAFAGTDTMRAAYRHAIETGYRFYSYGDSSLLWRA
EEECCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA