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The map label for this gene is miaA

Identifier: 197104340

GI number: 197104340

Start: 967492

End: 968415

Strand: Reverse

Name: miaA

Synonym: PHZ_c0874

Alternate gene names: 197104340

Gene position: 968415-967492 (Counterclockwise)

Preceding gene: 197104343

Following gene: 197104339

Centisome position: 24.23

GC content: 76.52

Gene sequence:

>924_bases
ATGGAGCCCCGCATCTGGCTGATCGCCGGGCCCACGGCAAGCGGCAAGTCGGCGCTGGCGCTCCGGCTGGCCGAGGCGAG
CGGGGCCGAGATCGTCAACGCCGACTCCATGCAGCTCTACGCGGGCCTGCGGGTGCTGACCGCCGGCCCCGGCCCCGAGG
AGACCGCCCGCGCGCCGCACCACCTGTTCGGGAGCGTCGACCCGGCGGACGGCTGGTCGGTGGGCCGATGGCTGCGGGCG
GCGAGCGAGGTCATCGCGGACATCCGCGGCCGCGGCCGCCCGGTCGTCGTCGTGGGCGGCACGGGGCTGTACTTCCGGGC
GCTGACACAGGGCCTGGCCGAGATCCCCGAGGTCCCGGCCGACGTCCGGGCCAAGGCCGCCGCCGACTTCGAGCGGATGG
GCGAGGCGGCCTTCCGCACCCGGCTGGCCGAGGTCGACCCCGCCGCCGCCGCCCGCATCGCCCCGGGCGACCGCCAGCGC
CTCTGCCGCGCCTGGGAGGTGTTCGCCGCGACGGGGCAGGCGCTCAGCGACCTGCAGCGCACCGGCGCACCGGCCATCGC
GGACTGGAGCGCCGTGGCCCTGGAGCCGCCCCGGCCGGCGCTCTACGCCCGCTGCGACGCGCGGCTGCACGCCATGGTCC
GCGAGGGGGCGCTGGAGGAGGTGCGCGCCCTGATCGCGCGGAACCTCGATCCCGCCCTGCCCGCCATGAAGGCCGTCGGG
GTGCGCGAATTCGCCGCCCACCTGCGCGGCGAGACCTCCCTGGAGGCGGCGGTGGAGGCGGCGCAGCAGGAGACCCGCCG
CTACGCCAAGCGCCAGATCACCTGGATGCGGGGCCAGATGGCCGGCTGGCCGCGCCTGACGGCAGATGACCACGAGGGCC
AGTGGAGGCAGTTTCTTGCGCAGGAACCCGGCTTGACGCCCTGA

Upstream 100 bases:

>100_bases
CGGGCCCAGCGGCCGGGGCGACTTGACGGGAAGGCCGGCGGCTGCGAGCGCTTCGGCCGCGGCGGGGGCGTCGCGGCCCA
CGAGTGTAAGGACAAGCTCC

Downstream 100 bases:

>100_bases
ACGCGAAATGCGAATGCACTTTGCAGTCTCTCCAGGGTAGTTACGTGCGCAACGACATCCTTGTACTCACCGGGCGGCCG
ACAACGGACTGACGATAGGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MEPRIWLIAGPTASGKSALALRLAEASGAEIVNADSMQLYAGLRVLTAGPGPEETARAPHHLFGSVDPADGWSVGRWLRA
ASEVIADIRGRGRPVVVVGGTGLYFRALTQGLAEIPEVPADVRAKAAADFERMGEAAFRTRLAEVDPAAAARIAPGDRQR
LCRAWEVFAATGQALSDLQRTGAPAIADWSAVALEPPRPALYARCDARLHAMVREGALEEVRALIARNLDPALPAMKAVG
VREFAAHLRGETSLEAAVEAAQQETRRYAKRQITWMRGQMAGWPRLTADDHEGQWRQFLAQEPGLTP

Sequences:

>Translated_307_residues
MEPRIWLIAGPTASGKSALALRLAEASGAEIVNADSMQLYAGLRVLTAGPGPEETARAPHHLFGSVDPADGWSVGRWLRA
ASEVIADIRGRGRPVVVVGGTGLYFRALTQGLAEIPEVPADVRAKAAADFERMGEAAFRTRLAEVDPAAAARIAPGDRQR
LCRAWEVFAATGQALSDLQRTGAPAIADWSAVALEPPRPALYARCDARLHAMVREGALEEVRALIARNLDPALPAMKAVG
VREFAAHLRGETSLEAAVEAAQQETRRYAKRQITWMRGQMAGWPRLTADDHEGQWRQFLAQEPGLTP
>Mature_307_residues
MEPRIWLIAGPTASGKSALALRLAEASGAEIVNADSMQLYAGLRVLTAGPGPEETARAPHHLFGSVDPADGWSVGRWLRA
ASEVIADIRGRGRPVVVVGGTGLYFRALTQGLAEIPEVPADVRAKAAADFERMGEAAFRTRLAEVDPAAAARIAPGDRQR
LCRAWEVFAATGQALSDLQRTGAPAIADWSAVALEPPRPALYARCDARLHAMVREGALEEVRALIARNLDPALPAMKAVG
VREFAAHLRGETSLEAAVEAAQQETRRYAKRQITWMRGQMAGWPRLTADDHEGQWRQFLAQEPGLTP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=29.4871794871795, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1790613, Length=279, Percent_Identity=41.2186379928315, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=25.6578947368421, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=28.135593220339, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24649830, Length=335, Percent_Identity=26.5671641791045, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PHEZH (B4RGS2)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129717.1
- ProteinModelPortal:   B4RGS2
- GeneID:   6790367
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0874
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33074; Mature: 33074

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPRIWLIAGPTASGKSALALRLAEASGAEIVNADSMQLYAGLRVLTAGPGPEETARAPH
CCCCEEEEECCCCCCCCCEEEEEECCCCCCEECCCCHHHHHCEEEEECCCCCHHHHCCCH
HLFGSVDPADGWSVGRWLRAASEVIADIRGRGRPVVVVGGTGLYFRALTQGLAEIPEVPA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCH
DVRAKAAADFERMGEAAFRTRLAEVDPAAAARIAPGDRQRLCRAWEVFAATGQALSDLQR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGAPAIADWSAVALEPPRPALYARCDARLHAMVREGALEEVRALIARNLDPALPAMKAVG
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
VREFAAHLRGETSLEAAVEAAQQETRRYAKRQITWMRGQMAGWPRLTADDHEGQWRQFLA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
QEPGLTP
CCCCCCC
>Mature Secondary Structure
MEPRIWLIAGPTASGKSALALRLAEASGAEIVNADSMQLYAGLRVLTAGPGPEETARAPH
CCCCEEEEECCCCCCCCCEEEEEECCCCCCEECCCCHHHHHCEEEEECCCCCHHHHCCCH
HLFGSVDPADGWSVGRWLRAASEVIADIRGRGRPVVVVGGTGLYFRALTQGLAEIPEVPA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCH
DVRAKAAADFERMGEAAFRTRLAEVDPAAAARIAPGDRQRLCRAWEVFAATGQALSDLQR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TGAPAIADWSAVALEPPRPALYARCDARLHAMVREGALEEVRALIARNLDPALPAMKAVG
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
VREFAAHLRGETSLEAAVEAAQQETRRYAKRQITWMRGQMAGWPRLTADDHEGQWRQFLA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
QEPGLTP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA