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The map label for this gene is ilvI [H]

Identifier: 197104339

GI number: 197104339

Start: 965511

End: 967244

Strand: Reverse

Name: ilvI [H]

Synonym: PHZ_c0873

Alternate gene names: 197104339

Gene position: 967244-965511 (Counterclockwise)

Preceding gene: 197104340

Following gene: 197104338

Centisome position: 24.2

GC content: 68.92

Gene sequence:

>1734_bases
GTGGTGCGCGCCCTGATCGACCAGGGCGTGGAGGTGCTGTTCGGCTATCCCGGCGGGGCGGTCCTGCCGATCTACGACGC
GCTGTTCGCCGAGCCGCGCCTGCGGCACGTGCTGGTGCGCCACGAGCAGGGCGCGACCCACGCGGCCGAGGGCTACGCCC
GCTCGACCGGCAAGCCCGGCGTCGTCCTGGTGACTTCGGGCCCCGGCGCGACCAATGCGGTCACCGGCATCGTCGACGCC
CTGATGGACTCCATCCCGATGGTCGTCATTACCGGCCAGGTCGCCACCCACCTGATCGGCACCGACGCCTTCCAGGAATG
CGACACCATCGGCATCACCCGGCCCTGCACCAAGCACAACTACCTGGTGAAGTCGGTCGAGGACCTGCCGCGGATCATCC
ACGAGGCGTTCCACATCGCCACCTCGGGCCGGCCGGGGCCGGTGGTCATCGACATCCCCAAGGACGTGCAGTTCGCCAAG
GGCGCCTACCGCGGCCCCGCCGAGGTGACCCACGCCCACAACTACGCCCCCCGCACGAAGGGCGAGCCGTCGAAGATCGC
CGAGGCCGTGGCGCTGCTGGCCAAGGCGCGGCGGCCGATCCTCTACACGGGCGGCGGCGTCATCAATTCCGGTCCCCGCG
CCGCCGAGCTGGTGCGCGAGCTGGCGGCCCTGACCGGCGCGCCGGTCACCTCGACCCTGATGGGCCTGGGCGCCTTCCCC
GCCTCCAGCCCGCAGTGGCTGGGCATGGTGGGCATGCACGGCTCGTTCGAGGCCAACAACGCCATGCACGACTGCGACGT
CATGGTGGCCATCGGCGCGCGCTTCGACGACCGGGTGACCGGCCGCCTGGACGCCTTCGCCCCCACCAGCCGGAAGATCC
ACATCGACGTGGACGCCAGCTCGATCGGCAAGAACGTGCGCGTCGACGTCGGCATCGTCGGCGACGCGGCCAGCGTGCTC
GAGGACCTGATCGGCGCCTGGAAGCAGCGGAACCTGAAGCTGGACAAGGCCGCGGTCGCCGACTGGTGGCGCGAGATCGA
GGCCTGGCGGGCGCGCGATTCCTTCCGCTACCGGCCGGATTCCAAGCTCATCAAGCCGCAGCACGCCATCCAGCGCCTCT
ACGAGCTGACCAAGGACCGGGACACCTACGTCACCACCGAAGTCGGCCAGCACCAGATGTGGGCGGCCCAGTTCTACCAC
TTCGACGAGCCGAACCGCTGGATGACCTCGGGCGGGCTCGGCACCATGGGCTACGGCCTGCCGGCCGCGGTGGGCGTGCA
GATCGCCCATCCGGACAGCCTGGTCATCGACATCGCAGGCGACGCCAGCGTGCAGATGACCATGCAGGAGATGTCGACGG
CGGTTCAGTACGACCTGCCGATCAAGATCTTCATCCTCAACAACGAGTGGATGGGGATGGTGCGCCAGTGGCAGCAGCTG
CTGCACGGCGAGCGCTACTCGCACTCGTATTCGGCCAGCCTGCCCGACTTCGTGAAGCTGGCCGAGGCCTACGGCTGCGT
CGGCCTGCGCGCCGAGCACCCCGACGAACTGGACGACGCGATCCGCGAGATGATCGACGTGCGCAAGCCGGTGATCTTCG
ACTGCCGGGTGACCAAGGAAGAGAACTGCTTCCCGATGATCCCCTCGGGCAAGGCGCACAACGAGATGATCATGGCCGAG
GAATCCCGCGACCTGGGCTCGATCATCGACGACGCGGGCAAGCGGCTGGTGTGA

Upstream 100 bases:

>100_bases
TATTGTCCTCACGTCCAGAGCCCCTCCCCCGAAAGGTCCGACCATGACCGCCCACCAGACGATCGAAGCCCAGCAGGACA
CCATCTCGGGCGCCGAGATC

Downstream 100 bases:

>100_bases
GCCTGGACGCGTACGAGGCGCTGGTCGCCCTCGCCCGGCGGCAGCAGGGCGAGGGCCGCTACCGCGAGTCCGAGGAGAGC
CTGAACCGGGCGCTGACCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MVRALIDQGVEVLFGYPGGAVLPIYDALFAEPRLRHVLVRHEQGATHAAEGYARSTGKPGVVLVTSGPGATNAVTGIVDA
LMDSIPMVVITGQVATHLIGTDAFQECDTIGITRPCTKHNYLVKSVEDLPRIIHEAFHIATSGRPGPVVIDIPKDVQFAK
GAYRGPAEVTHAHNYAPRTKGEPSKIAEAVALLAKARRPILYTGGGVINSGPRAAELVRELAALTGAPVTSTLMGLGAFP
ASSPQWLGMVGMHGSFEANNAMHDCDVMVAIGARFDDRVTGRLDAFAPTSRKIHIDVDASSIGKNVRVDVGIVGDAASVL
EDLIGAWKQRNLKLDKAAVADWWREIEAWRARDSFRYRPDSKLIKPQHAIQRLYELTKDRDTYVTTEVGQHQMWAAQFYH
FDEPNRWMTSGGLGTMGYGLPAAVGVQIAHPDSLVIDIAGDASVQMTMQEMSTAVQYDLPIKIFILNNEWMGMVRQWQQL
LHGERYSHSYSASLPDFVKLAEAYGCVGLRAEHPDELDDAIREMIDVRKPVIFDCRVTKEENCFPMIPSGKAHNEMIMAE
ESRDLGSIIDDAGKRLV

Sequences:

>Translated_577_residues
MVRALIDQGVEVLFGYPGGAVLPIYDALFAEPRLRHVLVRHEQGATHAAEGYARSTGKPGVVLVTSGPGATNAVTGIVDA
LMDSIPMVVITGQVATHLIGTDAFQECDTIGITRPCTKHNYLVKSVEDLPRIIHEAFHIATSGRPGPVVIDIPKDVQFAK
GAYRGPAEVTHAHNYAPRTKGEPSKIAEAVALLAKARRPILYTGGGVINSGPRAAELVRELAALTGAPVTSTLMGLGAFP
ASSPQWLGMVGMHGSFEANNAMHDCDVMVAIGARFDDRVTGRLDAFAPTSRKIHIDVDASSIGKNVRVDVGIVGDAASVL
EDLIGAWKQRNLKLDKAAVADWWREIEAWRARDSFRYRPDSKLIKPQHAIQRLYELTKDRDTYVTTEVGQHQMWAAQFYH
FDEPNRWMTSGGLGTMGYGLPAAVGVQIAHPDSLVIDIAGDASVQMTMQEMSTAVQYDLPIKIFILNNEWMGMVRQWQQL
LHGERYSHSYSASLPDFVKLAEAYGCVGLRAEHPDELDDAIREMIDVRKPVIFDCRVTKEENCFPMIPSGKAHNEMIMAE
ESRDLGSIIDDAGKRLV
>Mature_577_residues
MVRALIDQGVEVLFGYPGGAVLPIYDALFAEPRLRHVLVRHEQGATHAAEGYARSTGKPGVVLVTSGPGATNAVTGIVDA
LMDSIPMVVITGQVATHLIGTDAFQECDTIGITRPCTKHNYLVKSVEDLPRIIHEAFHIATSGRPGPVVIDIPKDVQFAK
GAYRGPAEVTHAHNYAPRTKGEPSKIAEAVALLAKARRPILYTGGGVINSGPRAAELVRELAALTGAPVTSTLMGLGAFP
ASSPQWLGMVGMHGSFEANNAMHDCDVMVAIGARFDDRVTGRLDAFAPTSRKIHIDVDASSIGKNVRVDVGIVGDAASVL
EDLIGAWKQRNLKLDKAAVADWWREIEAWRARDSFRYRPDSKLIKPQHAIQRLYELTKDRDTYVTTEVGQHQMWAAQFYH
FDEPNRWMTSGGLGTMGYGLPAAVGVQIAHPDSLVIDIAGDASVQMTMQEMSTAVQYDLPIKIFILNNEWMGMVRQWQQL
LHGERYSHSYSASLPDFVKLAEAYGCVGLRAEHPDELDDAIREMIDVRKPVIFDCRVTKEENCFPMIPSGKAHNEMIMAE
ESRDLGSIIDDAGKRLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=25.8527827648115, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI21361361, Length=571, Percent_Identity=25.0437828371278, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=51.5845070422535, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=42.6785714285714, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=33.6879432624113, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=28.7020109689214, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=25.8928571428571, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=518, Percent_Identity=27.2200772200772, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=498, Percent_Identity=26.9076305220884, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=498, Percent_Identity=26.9076305220884, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=48.5217391304348, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320816, Length=473, Percent_Identity=26.0042283298097, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922626, Length=478, Percent_Identity=26.9874476987448, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63077; Mature: 63077

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRALIDQGVEVLFGYPGGAVLPIYDALFAEPRLRHVLVRHEQGATHAAEGYARSTGKPG
CCHHHHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCHHCCCCCCC
VVLVTSGPGATNAVTGIVDALMDSIPMVVITGQVATHLIGTDAFQECDTIGITRPCTKHN
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHHHHHCCCCCCCCCCCCH
YLVKSVEDLPRIIHEAFHIATSGRPGPVVIDIPKDVQFAKGAYRGPAEVTHAHNYAPRTK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCCCCCHHHHHCCCCCCCCC
GEPSKIAEAVALLAKARRPILYTGGGVINSGPRAAELVRELAALTGAPVTSTLMGLGAFP
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCC
ASSPQWLGMVGMHGSFEANNAMHDCDVMVAIGARFDDRVTGRLDAFAPTSRKIHIDVDAS
CCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECHH
SIGKNVRVDVGIVGDAASVLEDLIGAWKQRNLKLDKAAVADWWREIEAWRARDSFRYRPD
HCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SKLIKPQHAIQRLYELTKDRDTYVTTEVGQHQMWAAQFYHFDEPNRWMTSGGLGTMGYGL
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHEECCCCCCEEECCCCCCCCCCC
PAAVGVQIAHPDSLVIDIAGDASVQMTMQEMSTAVQYDLPIKIFILNNEWMGMVRQWQQL
CHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHH
LHGERYSHSYSASLPDFVKLAEAYGCVGLRAEHPDELDDAIREMIDVRKPVIFDCRVTKE
HCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
ENCFPMIPSGKAHNEMIMAEESRDLGSIIDDAGKRLV
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MVRALIDQGVEVLFGYPGGAVLPIYDALFAEPRLRHVLVRHEQGATHAAEGYARSTGKPG
CCHHHHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHCHHCCCCCCC
VVLVTSGPGATNAVTGIVDALMDSIPMVVITGQVATHLIGTDAFQECDTIGITRPCTKHN
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHHHHHCCCCCCCCCCCCH
YLVKSVEDLPRIIHEAFHIATSGRPGPVVIDIPKDVQFAKGAYRGPAEVTHAHNYAPRTK
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCCCCCHHHHHCCCCCCCCC
GEPSKIAEAVALLAKARRPILYTGGGVINSGPRAAELVRELAALTGAPVTSTLMGLGAFP
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCC
ASSPQWLGMVGMHGSFEANNAMHDCDVMVAIGARFDDRVTGRLDAFAPTSRKIHIDVDAS
CCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECHH
SIGKNVRVDVGIVGDAASVLEDLIGAWKQRNLKLDKAAVADWWREIEAWRARDSFRYRPD
HCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SKLIKPQHAIQRLYELTKDRDTYVTTEVGQHQMWAAQFYHFDEPNRWMTSGGLGTMGYGL
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHEECCCCCCEEECCCCCCCCCCC
PAAVGVQIAHPDSLVIDIAGDASVQMTMQEMSTAVQYDLPIKIFILNNEWMGMVRQWQQL
CHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHH
LHGERYSHSYSASLPDFVKLAEAYGCVGLRAEHPDELDDAIREMIDVRKPVIFDCRVTKE
HCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
ENCFPMIPSGKAHNEMIMAEESRDLGSIIDDAGKRLV
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]