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The map label for this gene is rsgA2 [H]

Identifier: 197104244

GI number: 197104244

Start: 857990

End: 859072

Strand: Reverse

Name: rsgA2 [H]

Synonym: PHZ_c0778

Alternate gene names: 197104244

Gene position: 859072-857990 (Counterclockwise)

Preceding gene: 197104246

Following gene: 197104242

Centisome position: 21.5

GC content: 73.87

Gene sequence:

>1083_bases
GTGTTGACCCAATACGGCTGGAGCGACGAGCTCCAGCGCGACTTCGCGTCCTATGCGGCGCAGGGGCTCAAAGCCGGCCG
CGTCATCGTCCAGCAGCGCGGCGGCTATCGGCTGATCACCGAGGCCGGCGAGACCGACGCCAAGGCGGCCGGACGGCTGA
TGAAATCGCGGGAGGAGATGGCCCGCCCGGTGGCCGGCGACTGGGTCGCCTACGAGCCCCGCAAGGCGCCCGAGCCGGCG
TTCGTCCAGCACGTGCTGCCCAGGCGCACGGCCTTCGTGCGCAAGGCGGCGGGGCGGGGCGTGCACGCCCAGGTGGTGGC
GGCCAACGTGGACGTCGCCTTCCTGGTCACCTCGCTGAACTCGGACCTCAACCTGCGCCGGCTGGAGCGCTACCTCGCCA
CCGCCCACGAGAGCGGCGCCCGGCCGGTGATCGTGCTGACCAAGGCCGACCTCGCCCCCGACGCCGCCGAGCGCGTCGCC
GAGGTGCGGACCATCGCGGGCGAGGCGCCGGTGCTGGCCGTGGCCGCCAAGCAGGGCGAGGGGCTGGAAGCGGTCGCCGC
CCACCTGGAGCCCGGCCGCACCGCCGTGCTGCTGGGCATGTCGGGCGCGGGCAAGTCCACCCTGCTCAACGCCCTGGCCG
GGAGCGAGCGGATGGCCACCGGCGAGATCCGCGAGGACGACGAGCGCGGCCGGCACACCACCACCCACCGCGAGCTGGTG
CTGCTGCCGTCGGGGGGCCTGATCCTCGATACGCCCGGAATGCGCGAGCTGGGCCTGTGGGACGCCGACGCCGGGGTCTC
GGCGGTGTTCGAGGACATCGAGGCGCTGGTCGGCGAATGCCGGTTCAGCGACTGCGGGCACGGCGGCGAGCCCGGCTGCG
CCGTGCGGGCGGCCCTGGCGGACGGAAGCTTAGATCCCGACCGCTGGGCCAGCTTCCGCAAGCTGCAGGCCGAGGCCGCC
TACGAACAGCGCCGCGAGGACCCCCGCGCGGCGGCCGAGAACCGGAAGCTCTGGGCCGGCCGGCACAAGCAGGCCAAGGC
CTGGATCAAGCAGAAGCGCAGCTTCGGCGACGAGGAGTCCTAG

Upstream 100 bases:

>100_bases
TCGCCGTGACCGCCGCCTGAGGGCGGCCGATCTCTCCCCCTCACAGGATGGGTCCCCGCGGTCGACGCCACATCGGCTAT
CGACTTCAAGGACATCCCAA

Downstream 100 bases:

>100_bases
TTCCCCCTCAGGGGAAGCACCGGTCATGAAAAAGGCCCGGGGGTCGCCCCCGGGCCTTCCTATTCCGAATTTGCGCTGCG
CGATCAGTTGCGCTCCGTAT

Product: ribosome-associated GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLTQYGWSDELQRDFASYAAQGLKAGRVIVQQRGGYRLITEAGETDAKAAGRLMKSREEMARPVAGDWVAYEPRKAPEPA
FVQHVLPRRTAFVRKAAGRGVHAQVVAANVDVAFLVTSLNSDLNLRRLERYLATAHESGARPVIVLTKADLAPDAAERVA
EVRTIAGEAPVLAVAAKQGEGLEAVAAHLEPGRTAVLLGMSGAGKSTLLNALAGSERMATGEIREDDERGRHTTTHRELV
LLPSGGLILDTPGMRELGLWDADAGVSAVFEDIEALVGECRFSDCGHGGEPGCAVRAALADGSLDPDRWASFRKLQAEAA
YEQRREDPRAAAENRKLWAGRHKQAKAWIKQKRSFGDEES

Sequences:

>Translated_360_residues
MLTQYGWSDELQRDFASYAAQGLKAGRVIVQQRGGYRLITEAGETDAKAAGRLMKSREEMARPVAGDWVAYEPRKAPEPA
FVQHVLPRRTAFVRKAAGRGVHAQVVAANVDVAFLVTSLNSDLNLRRLERYLATAHESGARPVIVLTKADLAPDAAERVA
EVRTIAGEAPVLAVAAKQGEGLEAVAAHLEPGRTAVLLGMSGAGKSTLLNALAGSERMATGEIREDDERGRHTTTHRELV
LLPSGGLILDTPGMRELGLWDADAGVSAVFEDIEALVGECRFSDCGHGGEPGCAVRAALADGSLDPDRWASFRKLQAEAA
YEQRREDPRAAAENRKLWAGRHKQAKAWIKQKRSFGDEES
>Mature_360_residues
MLTQYGWSDELQRDFASYAAQGLKAGRVIVQQRGGYRLITEAGETDAKAAGRLMKSREEMARPVAGDWVAYEPRKAPEPA
FVQHVLPRRTAFVRKAAGRGVHAQVVAANVDVAFLVTSLNSDLNLRRLERYLATAHESGARPVIVLTKADLAPDAAERVA
EVRTIAGEAPVLAVAAKQGEGLEAVAAHLEPGRTAVLLGMSGAGKSTLLNALAGSERMATGEIREDDERGRHTTTHRELV
LLPSGGLILDTPGMRELGLWDADAGVSAVFEDIEALVGECRFSDCGHGGEPGCAVRAALADGSLDPDRWASFRKLQAEAA
YEQRREDPRAAAENRKLWAGRHKQAKAWIKQKRSFGDEES

Specific function: May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover [H]

COG id: COG1162

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 engC GTPase domain [H]

Homologues:

Organism=Escherichia coli, GI87082381, Length=311, Percent_Identity=30.5466237942122, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010914
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004881 [H]

Pfam domain/function: PF03193 DUF258 [H]

EC number: NA

Molecular weight: Translated: 38892; Mature: 38892

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50936 ENGC_GTPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQYGWSDELQRDFASYAAQGLKAGRVIVQQRGGYRLITEAGETDAKAAGRLMKSREEM
CCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARPVAGDWVAYEPRKAPEPAFVQHVLPRRTAFVRKAAGRGVHAQVVAANVDVAFLVTSLN
HCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC
SDLNLRRLERYLATAHESGARPVIVLTKADLAPDAAERVAEVRTIAGEAPVLAVAAKQGE
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
GLEAVAAHLEPGRTAVLLGMSGAGKSTLLNALAGSERMATGEIREDDERGRHTTTHRELV
CHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCEEE
LLPSGGLILDTPGMRELGLWDADAGVSAVFEDIEALVGECRFSDCGHGGEPGCAVRAALA
EEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
DGSLDPDRWASFRKLQAEAAYEQRREDPRAAAENRKLWAGRHKQAKAWIKQKRSFGDEES
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTQYGWSDELQRDFASYAAQGLKAGRVIVQQRGGYRLITEAGETDAKAAGRLMKSREEM
CCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARPVAGDWVAYEPRKAPEPAFVQHVLPRRTAFVRKAAGRGVHAQVVAANVDVAFLVTSLN
HCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC
SDLNLRRLERYLATAHESGARPVIVLTKADLAPDAAERVAEVRTIAGEAPVLAVAAKQGE
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
GLEAVAAHLEPGRTAVLLGMSGAGKSTLLNALAGSERMATGEIREDDERGRHTTTHRELV
CHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCEEE
LLPSGGLILDTPGMRELGLWDADAGVSAVFEDIEALVGECRFSDCGHGGEPGCAVRAALA
EEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
DGSLDPDRWASFRKLQAEAAYEQRREDPRAAAENRKLWAGRHKQAKAWIKQKRSFGDEES
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP [C]

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]