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The map label for this gene is gcvH

Identifier: 197104057

GI number: 197104057

Start: 625935

End: 626300

Strand: Reverse

Name: gcvH

Synonym: PHZ_c0591

Alternate gene names: 197104057

Gene position: 626300-625935 (Counterclockwise)

Preceding gene: 197104058

Following gene: 197104056

Centisome position: 15.67

GC content: 71.86

Gene sequence:

>366_bases
ATGCGCTTCACCAAGGATCACGAGTGGGTCGCCCTCGACGGCGACGTGGCCACCATCGGGATCACCGCCCACGCCGCCGA
GCAGCTCGGCGACGTGGTGTTCGTCGAGGTCCCCGAGGTCGGCCGCCAGGTGAAGGCCGGCGACGCCTTCGCCGTGGTCG
AGAGCGTCAAGGCCGCCTCCGACGTCTACGCCCCGGTCACCGGCGAGGTGGTCGAGGCGAACGAGGCCCTCTCGGGCGCC
CCCGAGACCGTCAACGCCGAGCCCGAGGCCGGCGGCTGGTTCGCCAAGGTCCGGGTCGCCGACCCCGCCGAGCTGGACGG
CCTGATGGACAAGGCCGCCTACGACGCCTTCGTCGCCAATTCCTGA

Upstream 100 bases:

>100_bases
CTCGCCCGCAAGCCCCCACCACCATGCTGCGCATGGTCCCCCTCCCCCGACGGGGGAGGAGGGCGCCAGGCCGCGCACCC
ATCCCCAGGAGCTAGACCCC

Downstream 100 bases:

>100_bases
CGCCGATCCCGTTCGTCATCCCACGGCCGGCCAAAGGCCGGTCCGGGGGACCCAAGCCGAGCCTCGAGCGCCGCATGGGT
CCCCCCGGATCGCTCCGCGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MRFTKDHEWVALDGDVATIGITAHAAEQLGDVVFVEVPEVGRQVKAGDAFAVVESVKAASDVYAPVTGEVVEANEALSGA
PETVNAEPEAGGWFAKVRVADPAELDGLMDKAAYDAFVANS

Sequences:

>Translated_121_residues
MRFTKDHEWVALDGDVATIGITAHAAEQLGDVVFVEVPEVGRQVKAGDAFAVVESVKAASDVYAPVTGEVVEANEALSGA
PETVNAEPEAGGWFAKVRVADPAELDGLMDKAAYDAFVANS
>Mature_121_residues
MRFTKDHEWVALDGDVATIGITAHAAEQLGDVVFVEVPEVGRQVKAGDAFAVVESVKAASDVYAPVTGEVVEANEALSGA
PETVNAEPEAGGWFAKVRVADPAELDGLMDKAAYDAFVANS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=44.5378151260504, Blast_Score=119, Evalue=4e-28,
Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=44.5378151260504, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=55.4621848739496, Blast_Score=140, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=44.3478260869565, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=45.2173913043478, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=119, Percent_Identity=38.655462184874, Blast_Score=97, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=44.6280991735537, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PHEZH (B4RF17)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129434.1
- GeneID:   6790082
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0591
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 12618; Mature: 12618

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTKDHEWVALDGDVATIGITAHAAEQLGDVVFVEVPEVGRQVKAGDAFAVVESVKAAS
CCCCCCCCEEEECCCEEEEEEEHHHHHHHCCEEEEECCCCCCEECCCCHHHHHHHHHHHH
DVYAPVTGEVVEANEALSGAPETVNAEPEAGGWFAKVRVADPAELDGLMDKAAYDAFVAN
HCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHEECC
S
C
>Mature Secondary Structure
MRFTKDHEWVALDGDVATIGITAHAAEQLGDVVFVEVPEVGRQVKAGDAFAVVESVKAAS
CCCCCCCCEEEECCCEEEEEEEHHHHHHHCCEEEEECCCCCCEECCCCHHHHHHHHHHHH
DVYAPVTGEVVEANEALSGAPETVNAEPEAGGWFAKVRVADPAELDGLMDKAAYDAFVAN
HCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHEECC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA