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The map label for this gene is maiA [H]

Identifier: 197103780

GI number: 197103780

Start: 306335

End: 306973

Strand: Direct

Name: maiA [H]

Synonym: PHZ_c0314

Alternate gene names: 197103780

Gene position: 306335-306973 (Clockwise)

Preceding gene: 197103779

Following gene: 197103781

Centisome position: 7.67

GC content: 73.24

Gene sequence:

>639_bases
ATGGCGCTCTCGCTGCACAGCTACTGGCGGGCCGGGGCGCCCTACCGGGTCCGCATCGGCCTGAACCTGAAGGGCCTGTC
CTACGACTACGTCCCGGTGAACCTGCTGGCCGGCGACCAGCACCGGCAGGCTTACAAGGCGCTCAACCCCCAGGGCCTGA
CGCCGGCCCTCGTCGTGGACGAGGCCCTGGTGCTGACCCAGAGCCTGGCCATCCTCGAATGGCTGGAGGAGACCCATCCC
GAGCCGCCGCTGCTGCCCAGGGCGGCCGCCGACCGCGCCGTGGTGCGCGCCATGGCCGGGATCGTGGCCTGCGACATCCA
TCCGCTGAACAACCAGCGCGTCCTGAAGGCCCTAGCGGGCCTGGGCGTGGACGAGGCGGGCCGCAACGCCTGGGCCGGCC
GCTGGATCGCCGCCGGCTTCGACGCGCTGGAGCCGATGATCGCCCGCCACGGGCGCGGCTTCGCCTTCGGCGAGACGCCG
ACCCTGGCCGACTGCTGCCTCATCCCGCAGGTCTATTCCGCCGGCCGCTTCGACGTGGACATGGAGCCTTGGCCCGCGAT
CCGCGCTGTGGCCGAGGCGGCCGCCGTTCACCCGGCCTTCGCCGCCGCCCGACCCGAACGCCAACCGGACGCCGCCTGA

Upstream 100 bases:

>100_bases
TCTTCACCGGCACGCCCGAGGGCGTCGGCCCGCTGGTCCGCGGCGACGGGGTCGAGGGCGAGGTCGACGGCGTCGGCGCC
CTGCAGTTCCGGGTGGTCTG

Downstream 100 bases:

>100_bases
TGCTCGACGAACTCAAGGAAAACAACCGCAACTGGGCCCAGGGGAAGGTCGCCGTCGACCCCGGCTTCTTCAAGCGCCTC
GAGGGCCAGCAGGCGCCCGA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MALSLHSYWRAGAPYRVRIGLNLKGLSYDYVPVNLLAGDQHRQAYKALNPQGLTPALVVDEALVLTQSLAILEWLEETHP
EPPLLPRAAADRAVVRAMAGIVACDIHPLNNQRVLKALAGLGVDEAGRNAWAGRWIAAGFDALEPMIARHGRGFAFGETP
TLADCCLIPQVYSAGRFDVDMEPWPAIRAVAEAAAVHPAFAAARPERQPDAA

Sequences:

>Translated_212_residues
MALSLHSYWRAGAPYRVRIGLNLKGLSYDYVPVNLLAGDQHRQAYKALNPQGLTPALVVDEALVLTQSLAILEWLEETHP
EPPLLPRAAADRAVVRAMAGIVACDIHPLNNQRVLKALAGLGVDEAGRNAWAGRWIAAGFDALEPMIARHGRGFAFGETP
TLADCCLIPQVYSAGRFDVDMEPWPAIRAVAEAAAVHPAFAAARPERQPDAA
>Mature_211_residues
ALSLHSYWRAGAPYRVRIGLNLKGLSYDYVPVNLLAGDQHRQAYKALNPQGLTPALVVDEALVLTQSLAILEWLEETHPE
PPLLPRAAADRAVVRAMAGIVACDIHPLNNQRVLKALAGLGVDEAGRNAWAGRWIAAGFDALEPMIARHGRGFAFGETPT
LADCCLIPQVYSAGRFDVDMEPWPAIRAVAEAAAVHPAFAAARPERQPDAA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=209, Percent_Identity=41.6267942583732, Blast_Score=164, Evalue=4e-41,
Organism=Homo sapiens, GI22202622, Length=157, Percent_Identity=40.7643312101911, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI194440732, Length=209, Percent_Identity=30.1435406698565, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=42.7884615384615, Blast_Score=167, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=36.3636363636364, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17556142, Length=198, Percent_Identity=39.3939393939394, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21355859, Length=209, Percent_Identity=41.1483253588517, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24645375, Length=209, Percent_Identity=41.1483253588517, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45553325, Length=209, Percent_Identity=41.1483253588517, Blast_Score=170, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21355857, Length=210, Percent_Identity=35.2380952380952, Blast_Score=140, Evalue=5e-34,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 22871; Mature: 22740

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSLHSYWRAGAPYRVRIGLNLKGLSYDYVPVNLLAGDQHRQAYKALNPQGLTPALVVD
CCCCCCHHHHCCCCEEEEEEEEECCCEECCEEEEEEECCHHHHHHHHCCCCCCCHHHHHH
EALVLTQSLAILEWLEETHPEPPLLPRAAADRAVVRAMAGIVACDIHPLNNQRVLKALAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHC
LGVDEAGRNAWAGRWIAAGFDALEPMIARHGRGFAFGETPTLADCCLIPQVYSAGRFDVD
CCCCCCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
MEPWPAIRAVAEAAAVHPAFAAARPERQPDAA
CCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ALSLHSYWRAGAPYRVRIGLNLKGLSYDYVPVNLLAGDQHRQAYKALNPQGLTPALVVD
CCCCCHHHHCCCCEEEEEEEEECCCEECCEEEEEEECCHHHHHHHHCCCCCCCHHHHHH
EALVLTQSLAILEWLEETHPEPPLLPRAAADRAVVRAMAGIVACDIHPLNNQRVLKALAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHC
LGVDEAGRNAWAGRWIAAGFDALEPMIARHGRGFAFGETPTLADCCLIPQVYSAGRFDVD
CCCCCCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
MEPWPAIRAVAEAAAVHPAFAAARPERQPDAA
CCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]