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The map label for this gene is proP [H]

Identifier: 197103755

GI number: 197103755

Start: 281711

End: 283387

Strand: Reverse

Name: proP [H]

Synonym: PHZ_c0289

Alternate gene names: 197103755

Gene position: 283387-281711 (Counterclockwise)

Preceding gene: 197103756

Following gene: 197103751

Centisome position: 7.09

GC content: 70.9

Gene sequence:

>1677_bases
ATGGCGGACGCGACCGCTGAGCGGGCCGGCGCGCCGGCGAAGGAAACCTCGCTGCGGACGGTCGTCGCCGCCTCGTCGGC
GGGCACGGCCTTCGAGTGGTACGACTTCTTCGTCTTCGGCAGCCTGGCCGGTGTCATCTCGCGCACCTTCTTCACCGGCC
TGCCCGAGACGGCGGGCCTGGCCGCGGCCCTGGCCCTGTTCGGCGCGGGCTTCCTGTTCCGCCCCCTGGGAGCCCTGGTG
TTCGGCCGGATCGGCGACAAGGCCGGCCGCAAGGGCGCGTTCCTGGCCACGGTGCTGCTGATGGGCGGGGCCACCTTCGC
CATCGGCCTGCTGCCCTCGTACGAGCAGGCCGGCCTGATCGGCCCGGCCCTGTTGATCCTGATGCGCATCCTGCAGGGCT
TCGCGCTGGGCGGCGAATACGGCGGGGCGGCGATCTACGTGGCCGAGCACGCGCCGCCGAACGGCCGCGGCTGGGCGACG
AGCTGGGTGCAGACCTCGGCGGCGCTGGGCCTGTTCGGGGCCCTGCTGGTGATCCTGGCCACCCGCTTGATCCTGGGCGA
ATTCGTCGGCGCCGAGGCCTTCGACGCCTGGGGCTGGCGCATCCCCTTCCTGTTCTCGGCGGGCCTGCTGGCCATCTCGG
TCTGGATGCGCCTGAAGCTCTCGGAGAGCCCGGCGTTCCAGAAGCTGAAGGACGAGGGCAACGTCCCGCACGCCCCGTTC
GCCGAGGCGTTCGGCCGCTGGGACAACCTGAAGATCGTGCTCCTGGCCCTGTTCGCCATCATGTCGGCCCAGGGCGCGGT
CTGGTACACGGCCTTCTTCTACGTCCAGACGTTCATGGAGCGGTTCCTGAAGGCGCCGCCCGAGACGATCACCGCCCTGA
TGCTCTCGGCCACCGCGATCAGCGCGGTGCTCTACGTCTTCTTCGGCTGGCTGTCGGACCGGGTCGGGCGCAAGCCGGTG
ATGCTGTTCGGCATGACGCTGATGCTGGCGGCCTACTTCCCCGGCTTCCACCTCCTGTCCAAGACCCTGAACCCGGCGCT
CGCCGAAGCCGAGGCCACGACGCCGGTGGTGGTGATCGCCGACCCCGCCGACTGCTCTTTGCAGTTCGATCCCGTCGGCA
AGGCCTCGTTCGCCTCGTCGTGCGACATCGCCAAGAACGCCCTGGCCAACGCCGGCGTCTCGTACCGCAACGAGGCGGGC
CCCGCCGGCCAGCCGGCGATGGTGCGGGTGGGCATGGCGACCATCGTCTCGGCCGACGCGCGCGGGCTCGCGCCCGAGGC
GACCAAGGCGGTGCGCGCCAGCGTCGAGGGCCGCATCCGGCAGGCCCTGGCCCGCGCCGGCTACCCTGAGAAGGCCGACC
CCGCGCGGCTGAACTTCTGGGGCGCGCTGGGGGTGATGGCGGTGTTCGTGATCGCCGCGACCGCGCTCTACGGCCCCATG
GCCGCCTGCCTGGTCGAGCTGTTCCCGACCCGCATCCGCTATACGGCCCTATCGCTGCCCTACCACATCGGCACCGGCTG
GATCGGCGGCTTCGTCCCCTTCAGCGCCTTCGCAATCGTGACGGCGGTGGGCGACATCTACTCGGGCCTGTGGTTCCCCT
TCGCCTTCACGGCGCTGTCGGTCGTGATCTGCTTCCTGTTCCTGAAGGAGACCAGGGGCCGGCCGCTGGACGCCTAG

Upstream 100 bases:

>100_bases
GGCCTGACCCCTCGCGCGCCGATCCGCCCCGACGCCTTCCACCCGTCGGCGAACCGGTGTTACAGCAGGCGCAACGACAC
CTTGGGGGGAGATGACGGGC

Downstream 100 bases:

>100_bases
CCCCCTTCTCCCCCTTGCGGGGGAAGGATCACCGCTTCCCGTCCAGCTCGGCGAGGGTGGCGCTGGAGGGCGGCCGCTCG
GCCTGCAGGCGCCGCGCGAC

Product: permease of the major facilitator superfamily

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MADATAERAGAPAKETSLRTVVAASSAGTAFEWYDFFVFGSLAGVISRTFFTGLPETAGLAAALALFGAGFLFRPLGALV
FGRIGDKAGRKGAFLATVLLMGGATFAIGLLPSYEQAGLIGPALLILMRILQGFALGGEYGGAAIYVAEHAPPNGRGWAT
SWVQTSAALGLFGALLVILATRLILGEFVGAEAFDAWGWRIPFLFSAGLLAISVWMRLKLSESPAFQKLKDEGNVPHAPF
AEAFGRWDNLKIVLLALFAIMSAQGAVWYTAFFYVQTFMERFLKAPPETITALMLSATAISAVLYVFFGWLSDRVGRKPV
MLFGMTLMLAAYFPGFHLLSKTLNPALAEAEATTPVVVIADPADCSLQFDPVGKASFASSCDIAKNALANAGVSYRNEAG
PAGQPAMVRVGMATIVSADARGLAPEATKAVRASVEGRIRQALARAGYPEKADPARLNFWGALGVMAVFVIAATALYGPM
AACLVELFPTRIRYTALSLPYHIGTGWIGGFVPFSAFAIVTAVGDIYSGLWFPFAFTALSVVICFLFLKETRGRPLDA

Sequences:

>Translated_558_residues
MADATAERAGAPAKETSLRTVVAASSAGTAFEWYDFFVFGSLAGVISRTFFTGLPETAGLAAALALFGAGFLFRPLGALV
FGRIGDKAGRKGAFLATVLLMGGATFAIGLLPSYEQAGLIGPALLILMRILQGFALGGEYGGAAIYVAEHAPPNGRGWAT
SWVQTSAALGLFGALLVILATRLILGEFVGAEAFDAWGWRIPFLFSAGLLAISVWMRLKLSESPAFQKLKDEGNVPHAPF
AEAFGRWDNLKIVLLALFAIMSAQGAVWYTAFFYVQTFMERFLKAPPETITALMLSATAISAVLYVFFGWLSDRVGRKPV
MLFGMTLMLAAYFPGFHLLSKTLNPALAEAEATTPVVVIADPADCSLQFDPVGKASFASSCDIAKNALANAGVSYRNEAG
PAGQPAMVRVGMATIVSADARGLAPEATKAVRASVEGRIRQALARAGYPEKADPARLNFWGALGVMAVFVIAATALYGPM
AACLVELFPTRIRYTALSLPYHIGTGWIGGFVPFSAFAIVTAVGDIYSGLWFPFAFTALSVVICFLFLKETRGRPLDA
>Mature_557_residues
ADATAERAGAPAKETSLRTVVAASSAGTAFEWYDFFVFGSLAGVISRTFFTGLPETAGLAAALALFGAGFLFRPLGALVF
GRIGDKAGRKGAFLATVLLMGGATFAIGLLPSYEQAGLIGPALLILMRILQGFALGGEYGGAAIYVAEHAPPNGRGWATS
WVQTSAALGLFGALLVILATRLILGEFVGAEAFDAWGWRIPFLFSAGLLAISVWMRLKLSESPAFQKLKDEGNVPHAPFA
EAFGRWDNLKIVLLALFAIMSAQGAVWYTAFFYVQTFMERFLKAPPETITALMLSATAISAVLYVFFGWLSDRVGRKPVM
LFGMTLMLAAYFPGFHLLSKTLNPALAEAEATTPVVVIADPADCSLQFDPVGKASFASSCDIAKNALANAGVSYRNEAGP
AGQPAMVRVGMATIVSADARGLAPEATKAVRASVEGRIRQALARAGYPEKADPARLNFWGALGVMAVFVIAATALYGPMA
ACLVELFPTRIRYTALSLPYHIGTGWIGGFVPFSAFAIVTAVGDIYSGLWFPFAFTALSVVICFLFLKETRGRPLDA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=345, Percent_Identity=35.3623188405797, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788292, Length=329, Percent_Identity=36.1702127659575, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789941, Length=340, Percent_Identity=35, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788942, Length=326, Percent_Identity=30.9815950920245, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082231, Length=180, Percent_Identity=28.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59123; Mature: 58991

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADATAERAGAPAKETSLRTVVAASSAGTAFEWYDFFVFGSLAGVISRTFFTGLPETAGL
CCCCCCHHCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AAALALFGAGFLFRPLGALVFGRIGDKAGRKGAFLATVLLMGGATFAIGLLPSYEQAGLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHEEECCCCHHHCCCH
GPALLILMRILQGFALGGEYGGAAIYVAEHAPPNGRGWATSWVQTSAALGLFGALLVILA
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TRLILGEFVGAEAFDAWGWRIPFLFSAGLLAISVWMRLKLSESPAFQKLKDEGNVPHAPF
HHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
AEAFGRWDNLKIVLLALFAIMSAQGAVWYTAFFYVQTFMERFLKAPPETITALMLSATAI
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SAVLYVFFGWLSDRVGRKPVMLFGMTLMLAAYFPGFHLLSKTLNPALAEAEATTPVVVIA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHCCCCCCEEEEE
DPADCSLQFDPVGKASFASSCDIAKNALANAGVSYRNEAGPAGQPAMVRVGMATIVSADA
CCCCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
RGLAPEATKAVRASVEGRIRQALARAGYPEKADPARLNFWGALGVMAVFVIAATALYGPM
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AACLVELFPTRIRYTALSLPYHIGTGWIGGFVPFSAFAIVTAVGDIYSGLWFPFAFTALS
HHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVICFLFLKETRGRPLDA
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADATAERAGAPAKETSLRTVVAASSAGTAFEWYDFFVFGSLAGVISRTFFTGLPETAGL
CCCCCHHCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AAALALFGAGFLFRPLGALVFGRIGDKAGRKGAFLATVLLMGGATFAIGLLPSYEQAGLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHEEECCCCHHHCCCH
GPALLILMRILQGFALGGEYGGAAIYVAEHAPPNGRGWATSWVQTSAALGLFGALLVILA
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TRLILGEFVGAEAFDAWGWRIPFLFSAGLLAISVWMRLKLSESPAFQKLKDEGNVPHAPF
HHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
AEAFGRWDNLKIVLLALFAIMSAQGAVWYTAFFYVQTFMERFLKAPPETITALMLSATAI
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SAVLYVFFGWLSDRVGRKPVMLFGMTLMLAAYFPGFHLLSKTLNPALAEAEATTPVVVIA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHCCCCCCEEEEE
DPADCSLQFDPVGKASFASSCDIAKNALANAGVSYRNEAGPAGQPAMVRVGMATIVSADA
CCCCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
RGLAPEATKAVRASVEGRIRQALARAGYPEKADPARLNFWGALGVMAVFVIAATALYGPM
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AACLVELFPTRIRYTALSLPYHIGTGWIGGFVPFSAFAIVTAVGDIYSGLWFPFAFTALS
HHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVICFLFLKETRGRPLDA
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]