Click here to switch to the map view.

The map label for this gene is sigW [H]

Identifier: 197103715

GI number: 197103715

Start: 249840

End: 250556

Strand: Direct

Name: sigW [H]

Synonym: PHZ_c0249

Alternate gene names: 197103715

Gene position: 249840-250556 (Clockwise)

Preceding gene: 197103706

Following gene: 197103728

Centisome position: 6.25

GC content: 75.45

Gene sequence:

>717_bases
ATGACAGCCCAACCCCATCACCTCGACATGGGGGCCCCGGACATGGCGGCCCTGAGCGAGGTCGAGCTCGTCGGCCGGGC
GCGCGGCGGCGATGCGGCCGCCTTCCGGGCCATCATGCAGGCCAGCAACCAGCGGCTCTTCCGGACCGCGCGCAGCATCG
TGCGCGACGAGTCCGAGGCCGAGGACGTCGTGCAGGAGGCCTATGCGAAGGCGTTCCAGCACCTCGACGGCTTCCGCGGC
GAGGCCCGGATCCTGACGTGGCTGACCCGGATCGTCGTCAACGAGGCGCGCGGCCGGCTGCGCAAGCGCCGCACGATGGT
GGACCTGTCCGCGATCGAGCAGGCCCAGGGCGAGGCCGGGCGGGTGGTGATGTTCCCGGGCGCGCTCGCCGCGGGCGATC
CCGAGCGCGAGGCGGCCAGGGCCGAGATGCGCCGGCTGCTGGAGCGGGCGCTCGACGGCCTGCCCGAGCCCCTGCGCCTG
GTGTTCATCCTGCGCGAGATCGAGGACTGCAGCGTCGCCGAGACGGCGCAGGCCCTCGGCGTGCGCGCAGAGACGGTGAA
GACGCGGCTGCACCGGGCGCGCCGGCAGCTGCGCCGCACGCTGGACGCCCAGCTGGCGGCGGCGGTCACCGAGGCCTTCC
CGTTCCTCGGCGCCCGCTGCGCGCGGATCACCGAGGCGGTGGTCGCCCGGCTCCGGGCGGCGGGCCTGGCGGCCTAG

Upstream 100 bases:

>100_bases
ATCGGCCCGCCGGCGCCGATTGGATGGCGCCCGGCCGGCGAGGTTCCCGGGAACCTTGCGGCCTGCGCCGCATCCGATGA
GAGTGACCCAGGAGCGCGCC

Downstream 100 bases:

>100_bases
ATCAGACCGCGGCGCGGTTCGCCGGCGGCTCGACCGACCAGATCTTCTCGAGATTGTAGAAGGCGCGCACTTCGGGCCGG
AACAGGTGGACGATCACGTC

Product: RNA polymerase sigma-70 factor,ECF subfamily

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTAQPHHLDMGAPDMAALSEVELVGRARGGDAAAFRAIMQASNQRLFRTARSIVRDESEAEDVVQEAYAKAFQHLDGFRG
EARILTWLTRIVVNEARGRLRKRRTMVDLSAIEQAQGEAGRVVMFPGALAAGDPEREAARAEMRRLLERALDGLPEPLRL
VFILREIEDCSVAETAQALGVRAETVKTRLHRARRQLRRTLDAQLAAAVTEAFPFLGARCARITEAVVARLRAAGLAA

Sequences:

>Translated_238_residues
MTAQPHHLDMGAPDMAALSEVELVGRARGGDAAAFRAIMQASNQRLFRTARSIVRDESEAEDVVQEAYAKAFQHLDGFRG
EARILTWLTRIVVNEARGRLRKRRTMVDLSAIEQAQGEAGRVVMFPGALAAGDPEREAARAEMRRLLERALDGLPEPLRL
VFILREIEDCSVAETAQALGVRAETVKTRLHRARRQLRRTLDAQLAAAVTEAFPFLGARCARITEAVVARLRAAGLAA
>Mature_237_residues
TAQPHHLDMGAPDMAALSEVELVGRARGGDAAAFRAIMQASNQRLFRTARSIVRDESEAEDVVQEAYAKAFQHLDGFRGE
ARILTWLTRIVVNEARGRLRKRRTMVDLSAIEQAQGEAGRVVMFPGALAAGDPEREAARAEMRRLLERALDGLPEPLRLV
FILREIEDCSVAETAQALGVRAETVKTRLHRARRQLRRTLDAQLAAAVTEAFPFLGARCARITEAVVARLRAAGLAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=181, Percent_Identity=34.8066298342541, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 26188; Mature: 26057

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQPHHLDMGAPDMAALSEVELVGRARGGDAAAFRAIMQASNQRLFRTARSIVRDESEA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EDVVQEAYAKAFQHLDGFRGEARILTWLTRIVVNEARGRLRKRRTMVDLSAIEQAQGEAG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RVVMFPGALAAGDPEREAARAEMRRLLERALDGLPEPLRLVFILREIEDCSVAETAQALG
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHH
VRAETVKTRLHRARRQLRRTLDAQLAAAVTEAFPFLGARCARITEAVVARLRAAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAQPHHLDMGAPDMAALSEVELVGRARGGDAAAFRAIMQASNQRLFRTARSIVRDESEA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EDVVQEAYAKAFQHLDGFRGEARILTWLTRIVVNEARGRLRKRRTMVDLSAIEQAQGEAG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RVVMFPGALAAGDPEREAARAEMRRLLERALDGLPEPLRLVFILREIEDCSVAETAQALG
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHH
VRAETVKTRLHRARRQLRRTLDAQLAAAVTEAFPFLGARCARITEAVVARLRAAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]