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The map label for this gene is gltD [H]

Identifier: 197103631

GI number: 197103631

Start: 160432

End: 161868

Strand: Reverse

Name: gltD [H]

Synonym: PHZ_c0165

Alternate gene names: 197103631

Gene position: 161868-160432 (Counterclockwise)

Preceding gene: 197103633

Following gene: 197103630

Centisome position: 4.05

GC content: 72.86

Gene sequence:

>1437_bases
ATGGCCGAGAAGATGCTGAAGTTCACGACGACGCCGCGCGCGACGCCCGAGAAGCGGCCGGCCATGGCGCGCTCGCACGA
CTTCCACGAGATCTACAAGGACTTCATCGACGCCAAGGCCGCCGAGCAGGCCTCGCGCTGCTCGCAGTGCGGCGTGCCGT
TCTGTCAGACCCACTGTCCGCTGCACAACAACATCCCCGACTGGCTGCGGATGACCGCCGAGGGCCGGCTCGAGGAGGCC
TATGCGCTGTCGCAGGCCACCAACTCCATGCCTGAGATCTGCGGCCGCATCTGCCCGCAGGACCGCCTGTGCGAGGGCAA
CTGCGTCATCGAGCAGTCGGGCCACGGCACCGTCACCATCGGCGCGGTCGAACGCTACCTGACCGACAAGGCCTGGGAGA
TGGGCTGGGTCAAGCCGCTGGTCCCCGGCGCCGACCGCGGCCAGTCGGTGGGGATCGTCGGCGCGGGCCCCGCGGGCCTG
GCCGCCGCCGAGCGCCTGCGCGAGATGGGCTACGCCGTCACCGTCTACGACCGGCACGACCGCGCCGGCGGTCTGCTGAT
CTACGGTATTCCCGGCTTCAAGCTGGAGAAGGACGTCGTCGAGCGCCGCACGGCGCGGCTGGCCAAGGGCGGCATCGAGT
TCCGGCTGGGCTTCGAGGTCGGCCGCGACGCCACCCTGGCCGAGCTGCGCGAGCGCCACGACGGGGTGCTGCTGGCGACC
GGCGTCTATGCGGCCCGCGACCTGACCGTGCCCGGCGCCGGCTCGGAGGGCGTCGTGCCCGCGCTCGAGTACCTGATCGC
CTCCAACCGCGAGCAGCTCGGCGACGGGGCGGTCGAGCCCCGCCTCAACGCCGCGGGCAAGCACGTCGTCGTGGTCGGCG
GCGGCGACACGGCCATGGACTGCGTGCGCACCGCCATCCGCCAGGACGCCGCCTCGGTGACCTGCCTCTACCGCCGCGAC
CGGGCCAACATGCCGGGCTCGGCCCGGGAGGTGGGCCACGCCGAGGAGGAGGGCGTGGTCTTCGAATGGCTGGCCGCCCC
CAAGGCGATCACCGGCGAGGCCCTGCAGGCCGCCGGCGTGCGCGCCGCGCGCATGCGCCTTGGCCCGCCCGACGCCTCGG
GCCGACAGAGCCCGGAGGAGATCGAGGGCGCCGACTTCGAACTGCAGGCCGAGATGGTCATCAAGGCGCTGGGCTTCGAC
CCCGAGGACCTGCCCGCCACCCTCAAAGCCGACGGCCTGTCGGTGACCCGCTGGGGCACCCTGAAGACCGACCTGCGCTC
GATGATGACCTCGATCCCCGGCGTGTTCGCCGCCGGCGACGTCGTCCGCGGCGCCAGCCTGGTGGTGTGGGCGATCCGCG
ACGGACGCGACGCCGCCGAGGCCATGCACCGCTACCTGACCGCCCACGCTGGGGCCGCCCTGATGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
GCAGCCCAGAACGATTCTGATAGTCCCGTTTTTGTATGATCATCTTTCCCGCCACGGCGCCGCCCCTTAAATGCGCGGCC
AACCGGGAGGAGGCGCCCCC

Downstream 100 bases:

>100_bases
CGCCGATGACCGACATCCTGACCCGCTACGAGACCGATCGGCAGCGCCTCCTGGAGGCCAACGGCGGCTACGACATCACC
GCCCAGCACGACGCCTGCGG

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAEKMLKFTTTPRATPEKRPAMARSHDFHEIYKDFIDAKAAEQASRCSQCGVPFCQTHCPLHNNIPDWLRMTAEGRLEEA
YALSQATNSMPEICGRICPQDRLCEGNCVIEQSGHGTVTIGAVERYLTDKAWEMGWVKPLVPGADRGQSVGIVGAGPAGL
AAAERLREMGYAVTVYDRHDRAGGLLIYGIPGFKLEKDVVERRTARLAKGGIEFRLGFEVGRDATLAELRERHDGVLLAT
GVYAARDLTVPGAGSEGVVPALEYLIASNREQLGDGAVEPRLNAAGKHVVVVGGGDTAMDCVRTAIRQDAASVTCLYRRD
RANMPGSAREVGHAEEEGVVFEWLAAPKAITGEALQAAGVRAARMRLGPPDASGRQSPEEIEGADFELQAEMVIKALGFD
PEDLPATLKADGLSVTRWGTLKTDLRSMMTSIPGVFAAGDVVRGASLVVWAIRDGRDAAEAMHRYLTAHAGAALMAAE

Sequences:

>Translated_478_residues
MAEKMLKFTTTPRATPEKRPAMARSHDFHEIYKDFIDAKAAEQASRCSQCGVPFCQTHCPLHNNIPDWLRMTAEGRLEEA
YALSQATNSMPEICGRICPQDRLCEGNCVIEQSGHGTVTIGAVERYLTDKAWEMGWVKPLVPGADRGQSVGIVGAGPAGL
AAAERLREMGYAVTVYDRHDRAGGLLIYGIPGFKLEKDVVERRTARLAKGGIEFRLGFEVGRDATLAELRERHDGVLLAT
GVYAARDLTVPGAGSEGVVPALEYLIASNREQLGDGAVEPRLNAAGKHVVVVGGGDTAMDCVRTAIRQDAASVTCLYRRD
RANMPGSAREVGHAEEEGVVFEWLAAPKAITGEALQAAGVRAARMRLGPPDASGRQSPEEIEGADFELQAEMVIKALGFD
PEDLPATLKADGLSVTRWGTLKTDLRSMMTSIPGVFAAGDVVRGASLVVWAIRDGRDAAEAMHRYLTAHAGAALMAAE
>Mature_477_residues
AEKMLKFTTTPRATPEKRPAMARSHDFHEIYKDFIDAKAAEQASRCSQCGVPFCQTHCPLHNNIPDWLRMTAEGRLEEAY
ALSQATNSMPEICGRICPQDRLCEGNCVIEQSGHGTVTIGAVERYLTDKAWEMGWVKPLVPGADRGQSVGIVGAGPAGLA
AAERLREMGYAVTVYDRHDRAGGLLIYGIPGFKLEKDVVERRTARLAKGGIEFRLGFEVGRDATLAELRERHDGVLLATG
VYAARDLTVPGAGSEGVVPALEYLIASNREQLGDGAVEPRLNAAGKHVVVVGGGDTAMDCVRTAIRQDAASVTCLYRRDR
ANMPGSAREVGHAEEEGVVFEWLAAPKAITGEALQAAGVRAARMRLGPPDASGRQSPEEIEGADFELQAEMVIKALGFDP
EDLPATLKADGLSVTRWGTLKTDLRSMMTSIPGVFAAGDVVRGASLVVWAIRDGRDAAEAMHRYLTAHAGAALMAAE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=481, Percent_Identity=25.987525987526, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI111118981, Length=169, Percent_Identity=30.1775147928994, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI111118983, Length=169, Percent_Identity=30.1775147928994, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789606, Length=476, Percent_Identity=45.1680672268908, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1788811, Length=471, Percent_Identity=46.2845010615711, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI87082180, Length=462, Percent_Identity=43.2900432900433, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=29.438202247191, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789243, Length=421, Percent_Identity=32.541567695962, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17570289, Length=510, Percent_Identity=34.9019607843137, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI71984108, Length=482, Percent_Identity=26.9709543568465, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17543860, Length=158, Percent_Identity=34.1772151898734, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=489, Percent_Identity=34.7648261758691, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=38.5714285714286, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=38.5714285714286, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=38.5714285714286, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=38.5714285714286, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24640763, Length=465, Percent_Identity=27.5268817204301, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI18858217, Length=465, Percent_Identity=27.5268817204301, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51319; Mature: 51188

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKMLKFTTTPRATPEKRPAMARSHDFHEIYKDFIDAKAAEQASRCSQCGVPFCQTHCP
CCCCCEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
LHNNIPDWLRMTAEGRLEEAYALSQATNSMPEICGRICPQDRLCEGNCVIEQSGHGTVTI
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEH
GAVERYLTDKAWEMGWVKPLVPGADRGQSVGIVGAGPAGLAAAERLREMGYAVTVYDRHD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCC
RAGGLLIYGIPGFKLEKDVVERRTARLAKGGIEFRLGFEVGRDATLAELRERHDGVLLAT
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEE
GVYAARDLTVPGAGSEGVVPALEYLIASNREQLGDGAVEPRLNAAGKHVVVVGGGDTAMD
CCEEEEEEECCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEECCCCHHHH
CVRTAIRQDAASVTCLYRRDRANMPGSAREVGHAEEEGVVFEWLAAPKAITGEALQAAGV
HHHHHHHHHHHHEEEEEECCCCCCCCCHHHCCCCCCCCEEEEEECCCCHHCHHHHHHHCC
RAARMRLGPPDASGRQSPEEIEGADFELQAEMVIKALGFDPEDLPATLKADGLSVTRWGT
HHHHHCCCCCCCCCCCCHHHHCCCCCEEHHHHHHHHHCCCCCCCCCEEECCCEEEEECCC
LKTDLRSMMTSIPGVFAAGDVVRGASLVVWAIRDGRDAAEAMHRYLTAHAGAALMAAE
HHHHHHHHHHHCCCHHHCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
AEKMLKFTTTPRATPEKRPAMARSHDFHEIYKDFIDAKAAEQASRCSQCGVPFCQTHCP
CCCCEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
LHNNIPDWLRMTAEGRLEEAYALSQATNSMPEICGRICPQDRLCEGNCVIEQSGHGTVTI
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEH
GAVERYLTDKAWEMGWVKPLVPGADRGQSVGIVGAGPAGLAAAERLREMGYAVTVYDRHD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCC
RAGGLLIYGIPGFKLEKDVVERRTARLAKGGIEFRLGFEVGRDATLAELRERHDGVLLAT
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEE
GVYAARDLTVPGAGSEGVVPALEYLIASNREQLGDGAVEPRLNAAGKHVVVVGGGDTAMD
CCEEEEEEECCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEECCCCHHHH
CVRTAIRQDAASVTCLYRRDRANMPGSAREVGHAEEEGVVFEWLAAPKAITGEALQAAGV
HHHHHHHHHHHHEEEEEECCCCCCCCCHHHCCCCCCCCEEEEEECCCCHHCHHHHHHHCC
RAARMRLGPPDASGRQSPEEIEGADFELQAEMVIKALGFDPEDLPATLKADGLSVTRWGT
HHHHHCCCCCCCCCCCCHHHHCCCCCEEHHHHHHHHHCCCCCCCCCEEECCCEEEEECCC
LKTDLRSMMTSIPGVFAAGDVVRGASLVVWAIRDGRDAAEAMHRYLTAHAGAALMAAE
HHHHHHHHHHHCCCHHHCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]