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The map label for this gene is leuB [H]

Identifier: 197103570

GI number: 197103570

Start: 95363

End: 96412

Strand: Reverse

Name: leuB [H]

Synonym: PHZ_c0104

Alternate gene names: 197103570

Gene position: 96412-95363 (Counterclockwise)

Preceding gene: 197103571

Following gene: 197103569

Centisome position: 2.41

GC content: 71.05

Gene sequence:

>1050_bases
GTGACCGATCTGCTTCTGCTTCCCGGCGACGGCATCGGACCGGAAGTGACCGCCCAGGTGCGCCGGGTCGCCGAGCGGCT
GGCGCCCGACCTCGCCATCGACGAGCGCCCGTTCGGCGGGACCAGCTACGACCGGCACGGGACGCCGCTGACCGACGAGG
CGCTGGCCCTGGCCAAGGCGTCGAAGGCCGTGCTGATGGGCGCCGTGGGCGGCCCCCAGTGGGCGGGCGTTCCGCGCGAC
AAGCGCCCGGAAGCGGGCCTGCTCGCCCTGCGCGCCGGCATGGAGGTGTTCGCGAACCTTCGCCCCGCCCTGTGCTTCGA
GCCGCTGGCCGACGCCTCGACGCTGAAGCGGGAGGTGGTCGAGGGCCTCGACATCATGATCGTGCGCGAGCTGATCGGCG
GCATCTACTTCGGCAGCCCACGCTTCATCGAGGAGATCCCGGGCGGCAAGCGGGCGGTGGACACCCAGGTCTACACCACC
CCCGAGATCGAGCGGATCGGTCGGGTCGCCTTCGAGCTCGCCCGCAAGCGCCGCAACAAGGTGACGAGCTGCGAGAAGTC
CAACGTGATGGACTCGGGCCTGCTCTGGCGCGAGGTGATCACCGACCTGCACCAGCGCGACTACGCGGACGTCCAGCTCG
AGCACATGCTGGCCGACAACGCCGCCATGCAGCTGGTGAAGAACCCGCGCCAGTTCGACGTCATCGTCACCGACAACCTG
TTCGGCGACATCCTCTCGGACGAGGCCTCGCAACTGACCGGCAGCCTGGGCCTGCTGCCCTCGGCCGCGCTGGGCGAGCC
CGGCCGTCCCGGCCTCTACGAGCCGATCCACGGCTCGGCGCCGGACATCGCCGGCAAGGGGATCGCCAATCCGCTGGCGG
CGATCCTGTCGTTCGAGATGGCGCTGCGCTGGTCGCTGGATCGGCCCGACCTGGCCGACCGGCTGTTCGCGGCCGTGGGC
CGGGCCCTGGAGAAGGGGGCGCGCACGCCGGATCTCGGCGGTAAGCTCACCACGATCCAGATGGGCGAGGCGGTGCTCGC
CGAACTCTAG

Upstream 100 bases:

>100_bases
CCGTGGCCATGCCGTGACGGGCCCGGGGTTCCCGAGCGCGCCGTCTTCGCCTAGGAGGTCGCCCGCGCACCTGGACGAAC
CGAGCCTTGGGGGACCTGCC

Downstream 100 bases:

>100_bases
CGGGAGGGGACGATGGCGGGCTGGCTGGTGAAGTCGGAACCCAACACCTACAGCTTCGAGGACCTGCAGCGCGACGGCCG
CACCGTCTGGGACGGCGTCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTDLLLLPGDGIGPEVTAQVRRVAERLAPDLAIDERPFGGTSYDRHGTPLTDEALALAKASKAVLMGAVGGPQWAGVPRD
KRPEAGLLALRAGMEVFANLRPALCFEPLADASTLKREVVEGLDIMIVRELIGGIYFGSPRFIEEIPGGKRAVDTQVYTT
PEIERIGRVAFELARKRRNKVTSCEKSNVMDSGLLWREVITDLHQRDYADVQLEHMLADNAAMQLVKNPRQFDVIVTDNL
FGDILSDEASQLTGSLGLLPSAALGEPGRPGLYEPIHGSAPDIAGKGIANPLAAILSFEMALRWSLDRPDLADRLFAAVG
RALEKGARTPDLGGKLTTIQMGEAVLAEL

Sequences:

>Translated_349_residues
MTDLLLLPGDGIGPEVTAQVRRVAERLAPDLAIDERPFGGTSYDRHGTPLTDEALALAKASKAVLMGAVGGPQWAGVPRD
KRPEAGLLALRAGMEVFANLRPALCFEPLADASTLKREVVEGLDIMIVRELIGGIYFGSPRFIEEIPGGKRAVDTQVYTT
PEIERIGRVAFELARKRRNKVTSCEKSNVMDSGLLWREVITDLHQRDYADVQLEHMLADNAAMQLVKNPRQFDVIVTDNL
FGDILSDEASQLTGSLGLLPSAALGEPGRPGLYEPIHGSAPDIAGKGIANPLAAILSFEMALRWSLDRPDLADRLFAAVG
RALEKGARTPDLGGKLTTIQMGEAVLAEL
>Mature_348_residues
TDLLLLPGDGIGPEVTAQVRRVAERLAPDLAIDERPFGGTSYDRHGTPLTDEALALAKASKAVLMGAVGGPQWAGVPRDK
RPEAGLLALRAGMEVFANLRPALCFEPLADASTLKREVVEGLDIMIVRELIGGIYFGSPRFIEEIPGGKRAVDTQVYTTP
EIERIGRVAFELARKRRNKVTSCEKSNVMDSGLLWREVITDLHQRDYADVQLEHMLADNAAMQLVKNPRQFDVIVTDNLF
GDILSDEASQLTGSLGLLPSAALGEPGRPGLYEPIHGSAPDIAGKGIANPLAAILSFEMALRWSLDRPDLADRLFAAVGR
ALEKGARTPDLGGKLTTIQMGEAVLAEL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=33.1428571428571, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=28.9693593314763, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI28178821, Length=362, Percent_Identity=28.7292817679558, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4758582, Length=355, Percent_Identity=28.4507042253521, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178838, Length=305, Percent_Identity=29.1803278688525, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI28178819, Length=205, Percent_Identity=32.6829268292683, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI87081683, Length=351, Percent_Identity=49.5726495726496, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1788101, Length=351, Percent_Identity=33.6182336182336, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=24.3718592964824, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=32.4858757062147, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=30.5475504322767, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=30.9659090909091, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=349, Percent_Identity=30.3724928366762, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=359, Percent_Identity=42.0612813370474, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322097, Length=360, Percent_Identity=34.7222222222222, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=31.4447592067989, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=354, Percent_Identity=34.180790960452, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24643268, Length=353, Percent_Identity=32.2946175637394, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643270, Length=353, Percent_Identity=32.2946175637394, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24661184, Length=355, Percent_Identity=29.8591549295775, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078633, Length=355, Percent_Identity=29.2957746478873, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650122, Length=355, Percent_Identity=29.2957746478873, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078635, Length=355, Percent_Identity=29.2957746478873, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078637, Length=355, Percent_Identity=29.2957746478873, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078639, Length=353, Percent_Identity=29.4617563739377, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=28.328611898017, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=28.328611898017, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20130355, Length=352, Percent_Identity=24.1477272727273, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37635; Mature: 37504

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLLLLPGDGIGPEVTAQVRRVAERLAPDLAIDERPFGGTSYDRHGTPLTDEALALAKA
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SKAVLMGAVGGPQWAGVPRDKRPEAGLLALRAGMEVFANLRPALCFEPLADASTLKREVV
HHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
EGLDIMIVRELIGGIYFGSPRFIEEIPGGKRAVDTQVYTTPEIERIGRVAFELARKRRNK
CCHHHHHHHHHHHHEECCCCHHHHHCCCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHH
VTSCEKSNVMDSGLLWREVITDLHQRDYADVQLEHMLADNAAMQLVKNPRQFDVIVTDNL
HHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCH
FGDILSDEASQLTGSLGLLPSAALGEPGRPGLYEPIHGSAPDIAGKGIANPLAAILSFEM
HHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALRWSLDRPDLADRLFAAVGRALEKGARTPDLGGKLTTIQMGEAVLAEL
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCC
>Mature Secondary Structure 
TDLLLLPGDGIGPEVTAQVRRVAERLAPDLAIDERPFGGTSYDRHGTPLTDEALALAKA
CCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SKAVLMGAVGGPQWAGVPRDKRPEAGLLALRAGMEVFANLRPALCFEPLADASTLKREVV
HHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHH
EGLDIMIVRELIGGIYFGSPRFIEEIPGGKRAVDTQVYTTPEIERIGRVAFELARKRRNK
CCHHHHHHHHHHHHEECCCCHHHHHCCCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHH
VTSCEKSNVMDSGLLWREVITDLHQRDYADVQLEHMLADNAAMQLVKNPRQFDVIVTDNL
HHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCH
FGDILSDEASQLTGSLGLLPSAALGEPGRPGLYEPIHGSAPDIAGKGIANPLAAILSFEM
HHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALRWSLDRPDLADRLFAAVGRALEKGARTPDLGGKLTTIQMGEAVLAEL
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]