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The map label for this gene is leuD

Identifier: 197103571

GI number: 197103571

Start: 97136

End: 97747

Strand: Reverse

Name: leuD

Synonym: PHZ_c0105

Alternate gene names: 197103571

Gene position: 97747-97136 (Counterclockwise)

Preceding gene: 197103572

Following gene: 197103570

Centisome position: 2.45

GC content: 67.48

Gene sequence:

>612_bases
ATGGAAGCCTTCACCCGCCTCGACGCCAAGGCCGCGCCGCTGCCGCTGGCCAACATCGACACCGACCAGATCATCCCCAA
GCAGTTCCTGAAGACGGTGGAGCGGGCGGGCCTGGCCAAGGGCCTGTTCTACGACCTGCGCTTCGACGAGCAGGGCCGCG
AGAAGCCGAACTTCGTCCTGAACCGGCCCGAGTACAAGGGCGCCGGCGTGCTGGTGGCCGGCGACAACTTCGGCTGCGGC
AGCTCGCGCGAGCACGCCCCGTGGGCGCTGATGGACTTCGGCATCCGCTGCGTGATCTCCACCAGCTTCGCCGATATCTT
CTACGGCAACTGCTTCCAGAACGGCCTGCTGCCGGTGGTGCTGAAGGCCGAGGAGGTCCAGCAGCTGATGGACGAGGCCC
GCGGCGGCAACCACGTGGTCAGCGTGGACCTCGAGGCCCAGACCGTCACGTCTCCGTCGGGGGCCGTGTTCCGCTTCGAG
ATCGATCCGCAGCGCAAGGACAAGATGCTCCGGGGCCTCGACGCCATCGGCGAGACCCTCCAGTCGCAGAAGGACATCGA
CGTCTACGAGATGAAGCGGGCGCTGGCCCAGCCTTGGCTGGAGGGGGCCTGA

Upstream 100 bases:

>100_bases
CCGGCCGCAAGCCCGGCACCCAGGTCTTCACCGTCCGCGACGCCCCCGCGGGCGTTCCGACCCTCATGCTCAGCGCCGAA
CCTGCGATGGAGACCGCCTG

Downstream 100 bases:

>100_bases
GGACCCCCTGAGGAACTTCCGCCGGACGGCGCGCGTCCGGCGGAGCTCGTCCTCTACTGCTTCTTCTCTTCGTCAGGTCC
GGCCTTGGCGCTCGCCGAGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MEAFTRLDAKAAPLPLANIDTDQIIPKQFLKTVERAGLAKGLFYDLRFDEQGREKPNFVLNRPEYKGAGVLVAGDNFGCG
SSREHAPWALMDFGIRCVISTSFADIFYGNCFQNGLLPVVLKAEEVQQLMDEARGGNHVVSVDLEAQTVTSPSGAVFRFE
IDPQRKDKMLRGLDAIGETLQSQKDIDVYEMKRALAQPWLEGA

Sequences:

>Translated_203_residues
MEAFTRLDAKAAPLPLANIDTDQIIPKQFLKTVERAGLAKGLFYDLRFDEQGREKPNFVLNRPEYKGAGVLVAGDNFGCG
SSREHAPWALMDFGIRCVISTSFADIFYGNCFQNGLLPVVLKAEEVQQLMDEARGGNHVVSVDLEAQTVTSPSGAVFRFE
IDPQRKDKMLRGLDAIGETLQSQKDIDVYEMKRALAQPWLEGA
>Mature_203_residues
MEAFTRLDAKAAPLPLANIDTDQIIPKQFLKTVERAGLAKGLFYDLRFDEQGREKPNFVLNRPEYKGAGVLVAGDNFGCG
SSREHAPWALMDFGIRCVISTSFADIFYGNCFQNGLLPVVLKAEEVQQLMDEARGGNHVVSVDLEAQTVTSPSGAVFRFE
IDPQRKDKMLRGLDAIGETLQSQKDIDVYEMKRALAQPWLEGA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=53.0612244897959, Blast_Score=189, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=210, Percent_Identity=50, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_PHEZH (B4RCC0)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002128948.1
- GeneID:   6789593
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0105
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22512; Mature: 22512

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFTRLDAKAAPLPLANIDTDQIIPKQFLKTVERAGLAKGLFYDLRFDEQGREKPNFVL
CCCHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCHHHCEEEEEEECCCCCCCCCEEE
NRPEYKGAGVLVAGDNFGCGSSREHAPWALMDFGIRCVISTSFADIFYGNCFQNGLLPVV
ECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCEEEEEECHHHHHHHHHHHHCCCEEEE
LKAEEVQQLMDEARGGNHVVSVDLEAQTVTSPSGAVFRFEIDPQRKDKMLRGLDAIGETL
ECHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
QSQKDIDVYEMKRALAQPWLEGA
HCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEAFTRLDAKAAPLPLANIDTDQIIPKQFLKTVERAGLAKGLFYDLRFDEQGREKPNFVL
CCCHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCHHHCEEEEEEECCCCCCCCCEEE
NRPEYKGAGVLVAGDNFGCGSSREHAPWALMDFGIRCVISTSFADIFYGNCFQNGLLPVV
ECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCEEEEEECHHHHHHHHHHHHCCCEEEE
LKAEEVQQLMDEARGGNHVVSVDLEAQTVTSPSGAVFRFEIDPQRKDKMLRGLDAIGETL
ECHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
QSQKDIDVYEMKRALAQPWLEGA
HCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA