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The map label for this gene is hisF [H]

Identifier: 197103509

GI number: 197103509

Start: 36014

End: 36784

Strand: Direct

Name: hisF [H]

Synonym: PHZ_c0043

Alternate gene names: 197103509

Gene position: 36014-36784 (Clockwise)

Preceding gene: 197103508

Following gene: 197103510

Centisome position: 0.9

GC content: 70.56

Gene sequence:

>771_bases
CTGATGCTGAACGTGCGCGTCATCCCCTGCCTGGACGTGAAGGACGGGCGCGTGGTGAAGGGCGTGCAGTTCGTCTCGCT
GCGGGACGCGGGCGATCCGGTCGAGCAGGCCAGGGCCTACGACGCCGCGGGCGCCGATGAGCTGATGTTCCTCGACATCA
CCGCCAGCCACGAGAACCGTGGCACCATCTTCGACGTGGTGGCCCGCACGGCCGAGGTCTGCTTCATGCCCCTGTCGGTG
GGCGGCGGCATCCGCTCGGTGGAGGACGCCCGGCGCCTGCTGCTGGCCGGGGCCGACAAGATCAGCATCAACACCGCCGC
GGTGAAGGACCGGAGCCTGATCTCGGGCTGCGCCGACGCCTTCGGCAGCCAGGCGACGGTGGTGGCCATCGACGCCAAGA
AGGTCGGGGACCGCTGGCGCGTCTTCATCTACGGCGGCCGCGAGGACACCGGCCTCGACGTGATGGAATACGCCCAGGAC
GTGGTGAAGCACGGCGCAGGCGAGATCCTGCTGACGTCCATGGACCGCGACGGGGCCAAGACCGGCTACGACCTGGAGCT
GCTGCGCGCCGTGTCCTCGGCCGTCGGCGTGCCGGTGATCGCCTCGGGCGGGGCGGGCAATGCGCAGCACATGGTGGACG
CAGTGCGGGCCGGCGCCGATGCGGTGCTAGCGGCCTCGATCTTCCACTTCGGCGAGGTGTCCATCGGCGAGGTCAAGGCC
GCCATGGCCGCCGAGGGCATTCCCGTCCGCCTGACCGAGAGGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCTGAAGGCCCGCAAGGGCGCGCCGATCGCCGGCGCGGTGCTGGGCCGGGCGCTCTACAACGGCGCCATCGACCCGGCC
GAGGCGCTGAAGGTGGCCGC

Downstream 100 bases:

>100_bases
TGCCGCACCAGCGCTTCGCCGAGGTGATCGGCCGCCTCGCCGCCACGATCGAGAGCCGCCGGGGCGGGGACGCCTCGGCC
TCGTATACGGCCCAGCTGCT

Product: hisF protein (cyclase)

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MMLNVRVIPCLDVKDGRVVKGVQFVSLRDAGDPVEQARAYDAAGADELMFLDITASHENRGTIFDVVARTAEVCFMPLSV
GGGIRSVEDARRLLLAGADKISINTAAVKDRSLISGCADAFGSQATVVAIDAKKVGDRWRVFIYGGREDTGLDVMEYAQD
VVKHGAGEILLTSMDRDGAKTGYDLELLRAVSSAVGVPVIASGGAGNAQHMVDAVRAGADAVLAASIFHFGEVSIGEVKA
AMAAEGIPVRLTERAA

Sequences:

>Translated_256_residues
MMLNVRVIPCLDVKDGRVVKGVQFVSLRDAGDPVEQARAYDAAGADELMFLDITASHENRGTIFDVVARTAEVCFMPLSV
GGGIRSVEDARRLLLAGADKISINTAAVKDRSLISGCADAFGSQATVVAIDAKKVGDRWRVFIYGGREDTGLDVMEYAQD
VVKHGAGEILLTSMDRDGAKTGYDLELLRAVSSAVGVPVIASGGAGNAQHMVDAVRAGADAVLAASIFHFGEVSIGEVKA
AMAAEGIPVRLTERAA
>Mature_256_residues
MMLNVRVIPCLDVKDGRVVKGVQFVSLRDAGDPVEQARAYDAAGADELMFLDITASHENRGTIFDVVARTAEVCFMPLSV
GGGIRSVEDARRLLLAGADKISINTAAVKDRSLISGCADAFGSQATVVAIDAKKVGDRWRVFIYGGREDTGLDVMEYAQD
VVKHGAGEILLTSMDRDGAKTGYDLELLRAVSSAVGVPVIASGGAGNAQHMVDAVRAGADAVLAASIFHFGEVSIGEVKA
AMAAEGIPVRLTERAA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=47.8599221789883, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082028, Length=207, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=35.0157728706625, Blast_Score=156, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26836; Mature: 26836

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLNVRVIPCLDVKDGRVVKGVQFVSLRDAGDPVEQARAYDAAGADELMFLDITASHENR
CEEEEEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
GTIFDVVARTAEVCFMPLSVGGGIRSVEDARRLLLAGADKISINTAAVKDRSLISGCADA
CHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHHH
FGSQATVVAIDAKKVGDRWRVFIYGGREDTGLDVMEYAQDVVKHGAGEILLTSMDRDGAK
CCCCEEEEEEEHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
TGYDLELLRAVSSAVGVPVIASGGAGNAQHMVDAVRAGADAVLAASIFHFGEVSIGEVKA
CCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHH
AMAAEGIPVRLTERAA
HHHCCCCCEEEECCCC
>Mature Secondary Structure
MMLNVRVIPCLDVKDGRVVKGVQFVSLRDAGDPVEQARAYDAAGADELMFLDITASHENR
CEEEEEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
GTIFDVVARTAEVCFMPLSVGGGIRSVEDARRLLLAGADKISINTAAVKDRSLISGCADA
CHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHHH
FGSQATVVAIDAKKVGDRWRVFIYGGREDTGLDVMEYAQDVVKHGAGEILLTSMDRDGAK
CCCCEEEEEEEHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
TGYDLELLRAVSSAVGVPVIASGGAGNAQHMVDAVRAGADAVLAASIFHFGEVSIGEVKA
CCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHH
AMAAEGIPVRLTERAA
HHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA