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The map label for this gene is hisH [H]

Identifier: 197103507

GI number: 197103507

Start: 34663

End: 35298

Strand: Direct

Name: hisH [H]

Synonym: PHZ_c0041

Alternate gene names: 197103507

Gene position: 34663-35298 (Clockwise)

Preceding gene: 197103506

Following gene: 197103508

Centisome position: 0.87

GC content: 71.86

Gene sequence:

>636_bases
ATGCAGAGGGTGGCCCTGATCGACTACGGGTCGGGCAACCTGCGCTCGGCCGAAAAGGCCCTCGTCCGCGCCGCGGACGG
GCGGGCGGAGATCGTCGTGACGCACGATCCCGGGTTCATCGCGGCCGCCGACCGCGTGGTCCTGCCGGGCGTCGGCGCCT
TCGCCGCCTGCATGGGCGCGCTCGAGGCGCGGACGGGCGTGGTCGAGGCCATGACGCAGGCGGTGCGTACGCGCGGCGTC
CCGTTCCTCGGCATCTGCGTCGGCATGCAGCTGATGGCCACGCGGGGCCTGGAGTTCGGCGAGACCGCCGGCCTGGACTG
GATCGCCGGCGACGTGCGCCGGATCGAGCCGGACGACCCCGCCGCGAAGGTGCCCCACATGGGCTGGAACGCCCTCCTGG
ATGTGATCGACCATCCGGTCTTCCGCGGCCTGAAGGACGGACGGCCGGTCTATTTCACCCACTCCTTCGCGTTCTTCGCC
AAGGACCCCGCGGATGTCGCCGCCTATGTCGACCACGGGGGACGCTTCCCGGCGGCCGTCGCGCGCGGCAACATGGCCGG
CGTCCAGTTCCACCCGGAGAAGTCGCAAGGCGCCGGCCTCTCCCTCCTCGCCGATTTCCTGGAGTGGCGCCCTTGA

Upstream 100 bases:

>100_bases
TTCAAGGCGCTGGCCCGGGCCCTGCGGCAGGCGGTCGAGCTCGATCCCAAGACCCACGGCCACGCCCCCTCCACCAAGGG
CGTCCTCTAGGATCATCGCG

Downstream 100 bases:

>100_bases
TCCTCTATCCCGCCATCGACCTGAAGGACGGACAGTGCGTCCGGGTGCTTCACGGCGATCTTTCCACGGCCACGGTGTTC
AACACCGACCCCGGCGCCCA

Product: amidotransferase

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MQRVALIDYGSGNLRSAEKALVRAADGRAEIVVTHDPGFIAAADRVVLPGVGAFAACMGALEARTGVVEAMTQAVRTRGV
PFLGICVGMQLMATRGLEFGETAGLDWIAGDVRRIEPDDPAAKVPHMGWNALLDVIDHPVFRGLKDGRPVYFTHSFAFFA
KDPADVAAYVDHGGRFPAAVARGNMAGVQFHPEKSQGAGLSLLADFLEWRP

Sequences:

>Translated_211_residues
MQRVALIDYGSGNLRSAEKALVRAADGRAEIVVTHDPGFIAAADRVVLPGVGAFAACMGALEARTGVVEAMTQAVRTRGV
PFLGICVGMQLMATRGLEFGETAGLDWIAGDVRRIEPDDPAAKVPHMGWNALLDVIDHPVFRGLKDGRPVYFTHSFAFFA
KDPADVAAYVDHGGRFPAAVARGNMAGVQFHPEKSQGAGLSLLADFLEWRP
>Mature_211_residues
MQRVALIDYGSGNLRSAEKALVRAADGRAEIVVTHDPGFIAAADRVVLPGVGAFAACMGALEARTGVVEAMTQAVRTRGV
PFLGICVGMQLMATRGLEFGETAGLDWIAGDVRRIEPDDPAAKVPHMGWNALLDVIDHPVFRGLKDGRPVYFTHSFAFFA
KDPADVAAYVDHGGRFPAAVARGNMAGVQFHPEKSQGAGLSLLADFLEWRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=37.9807692307692, Blast_Score=122, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=31.5068493150685, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22499; Mature: 22499

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVALIDYGSGNLRSAEKALVRAADGRAEIVVTHDPGFIAAADRVVLPGVGAFAACMGA
CCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEECCEEEECCCHHHHHHHHH
LEARTGVVEAMTQAVRTRGVPFLGICVGMQLMATRGLEFGETAGLDWIAGDVRRIEPDDP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCC
AAKVPHMGWNALLDVIDHPVFRGLKDGRPVYFTHSFAFFAKDPADVAAYVDHGGRFPAAV
CCCCCCCCHHHHHHHHCCHHHCCCCCCCEEEEEEEEEEEECCHHHHHHHHHCCCCCCHHH
ARGNMAGVQFHPEKSQGAGLSLLADFLEWRP
HCCCCCCEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQRVALIDYGSGNLRSAEKALVRAADGRAEIVVTHDPGFIAAADRVVLPGVGAFAACMGA
CCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEECCEEEECCCHHHHHHHHH
LEARTGVVEAMTQAVRTRGVPFLGICVGMQLMATRGLEFGETAGLDWIAGDVRRIEPDDP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCC
AAKVPHMGWNALLDVIDHPVFRGLKDGRPVYFTHSFAFFAKDPADVAAYVDHGGRFPAAV
CCCCCCCCHHHHHHHHCCHHHCCCCCCCEEEEEEEEEEEECCHHHHHHHHHCCCCCCHHH
ARGNMAGVQFHPEKSQGAGLSLLADFLEWRP
HCCCCCCEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]