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The map label for this gene is dacA.2 [H]

Identifier: 195978797

GI number: 195978797

Start: 1748299

End: 1749534

Strand: Direct

Name: dacA.2 [H]

Synonym: Sez_1699

Alternate gene names: 195978797

Gene position: 1748299-1749534 (Clockwise)

Preceding gene: 195978796

Following gene: 195978798

Centisome position: 86.37

GC content: 44.09

Gene sequence:

>1236_bases
ATGAAAAAACTTATTGTGACACTTGCTGCGCTCATGTGTCTGATCACAAATACCTCCCTTGCTGAGGACTTCTCTGTTAA
TAGTCGTCAAGCCATTGCGGTTGAAATGACAACTGGAAAAATCCTGTATGCCAAAGAGGCCCAGCAAAAGGCACCTATTG
CTTCGCTAACAAAGCTACTAACCGTCTACCTAGTCCTGAAGGAAATTAAGGCAGGACGGCTCCAGTGGGATAGTCAGGTA
ATCCTATCTGAATATGCTCTGCAATTAGCCACTGATCCTGATATCAGCAACCCAGCATTTGAGCAAAGACCTTACACTAT
CAAAGAGCTTGTTGAATCTAGCCTGATCATTAGTGCTAATAGCTCTGCTGTAGCTCTAGCAGAGCATATCGCTGGCTCAG
AGCCTACATTTGTAGATATGATGAAAAAACAGCTGGAGGCTTGGGGGATTAGAGACTATCGTCTTGTCAATGCTTCAGGC
CTAAACAACAGCATGCTAAAGGGGCATCTATATCCAGGCTCACAGCCAGACGATGAAAATCAGATGACAGCTAGAGATCT
GGCACTGATCGCACAACAGCTGCTTCAGGACTTTCCTGAGGTTCTCACCATCTCCTCACAGGCCCAATTGAAATGGGGTG
AGAAGGATTTGCCTAACTCTAATCTCCTATTAGCCGGACGCGAAATGGCTAGAACTGGGGTTGATGGCCTTAAAACAGGT
ACTACCAGCCAAGCCGGACAAACAATGATTGCAACAGCTGTGCAGGATAATATGCGAGTTATTTCTGTCATTCTCCATGC
TGATCAATCTGACACTGATGAGCGAGCTCGCTTTGTTGAGACCAATCGCCTGCTTGATTATTGCTTTAATAGCTATACCT
TAGAAAAGCTTGTTGCTCAAAATCAAGTTTTGCCTGAAAGGCTAATTGTTAAACAAGCAGCAAAGCAAAGGCTAAGGGTC
AAGGCTAATCAAAGCTTGATGGTTGTTCGGCCTAAGGATCAGACACAGCTGTCTTATTCGCTACGAATCAAGGACAAAGT
ACTCAAGACCCCGATTCGCCAAAATAAAAAGGTCGGTACCCTTATCTATCAGGATCCTAAGCTCATTGACAGTGGCTATC
TAGCAGCACCGCCAGCTGTTGCTATTGTCTCAGCTAAAAGCATTCCTGAAAAAGGGAAACTTAGCAAATTATTTGACTTT
TTTCAAAAAGTCATTGGCATTCTCATTAACCCTTAA

Upstream 100 bases:

>100_bases
TTTTGGAGGACGTCATCTCACTCAAAATCTTGATCTAGCCTTCTTTAAAGCTACTCAATGACCTTTTGACCGATCACTAT
TTCCTTGCTACAATAGTGGT

Downstream 100 bases:

>100_bases
TATTTTCAAAAAATATATTTCATTTGTATTATTGAAATGATATAATAAGCTTACATTATTTTTTTACATTAAGGAGCTTT
GAATTGACTAAAAGATTGCT

Product: D-alanyl-D-alanine carboxypeptidase precursor DacA.2

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKKLIVTLAALMCLITNTSLAEDFSVNSRQAIAVEMTTGKILYAKEAQQKAPIASLTKLLTVYLVLKEIKAGRLQWDSQV
ILSEYALQLATDPDISNPAFEQRPYTIKELVESSLIISANSSAVALAEHIAGSEPTFVDMMKKQLEAWGIRDYRLVNASG
LNNSMLKGHLYPGSQPDDENQMTARDLALIAQQLLQDFPEVLTISSQAQLKWGEKDLPNSNLLLAGREMARTGVDGLKTG
TTSQAGQTMIATAVQDNMRVISVILHADQSDTDERARFVETNRLLDYCFNSYTLEKLVAQNQVLPERLIVKQAAKQRLRV
KANQSLMVVRPKDQTQLSYSLRIKDKVLKTPIRQNKKVGTLIYQDPKLIDSGYLAAPPAVAIVSAKSIPEKGKLSKLFDF
FQKVIGILINP

Sequences:

>Translated_411_residues
MKKLIVTLAALMCLITNTSLAEDFSVNSRQAIAVEMTTGKILYAKEAQQKAPIASLTKLLTVYLVLKEIKAGRLQWDSQV
ILSEYALQLATDPDISNPAFEQRPYTIKELVESSLIISANSSAVALAEHIAGSEPTFVDMMKKQLEAWGIRDYRLVNASG
LNNSMLKGHLYPGSQPDDENQMTARDLALIAQQLLQDFPEVLTISSQAQLKWGEKDLPNSNLLLAGREMARTGVDGLKTG
TTSQAGQTMIATAVQDNMRVISVILHADQSDTDERARFVETNRLLDYCFNSYTLEKLVAQNQVLPERLIVKQAAKQRLRV
KANQSLMVVRPKDQTQLSYSLRIKDKVLKTPIRQNKKVGTLIYQDPKLIDSGYLAAPPAVAIVSAKSIPEKGKLSKLFDF
FQKVIGILINP
>Mature_411_residues
MKKLIVTLAALMCLITNTSLAEDFSVNSRQAIAVEMTTGKILYAKEAQQKAPIASLTKLLTVYLVLKEIKAGRLQWDSQV
ILSEYALQLATDPDISNPAFEQRPYTIKELVESSLIISANSSAVALAEHIAGSEPTFVDMMKKQLEAWGIRDYRLVNASG
LNNSMLKGHLYPGSQPDDENQMTARDLALIAQQLLQDFPEVLTISSQAQLKWGEKDLPNSNLLLAGREMARTGVDGLKTG
TTSQAGQTMIATAVQDNMRVISVILHADQSDTDERARFVETNRLLDYCFNSYTLEKLVAQNQVLPERLIVKQAAKQRLRV
KANQSLMVVRPKDQTQLSYSLRIKDKVLKTPIRQNKKVGTLIYQDPKLIDSGYLAAPPAVAIVSAKSIPEKGKLSKLFDF
FQKVIGILINP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=408, Percent_Identity=26.9607843137255, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082022, Length=424, Percent_Identity=28.5377358490566, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786851, Length=424, Percent_Identity=25, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45544; Mature: 45544

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIVTLAALMCLITNTSLAEDFSVNSRQAIAVEMTTGKILYAKEAQQKAPIASLTKLL
CHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCEEEEECCHHHHCCHHHHHHHH
TVYLVLKEIKAGRLQWDSQVILSEYALQLATDPDISNPAFEQRPYTIKELVESSLIISAN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCEEEECC
SSAVALAEHIAGSEPTFVDMMKKQLEAWGIRDYRLVNASGLNNSMLKGHLYPGSQPDDEN
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCC
QMTARDLALIAQQLLQDFPEVLTISSQAQLKWGEKDLPNSNLLLAGREMARTGVDGLKTG
HHHHHHHHHHHHHHHHHHHHHEECCCCHHCCCCCCCCCCCCEEEECHHHHHCCCCCCCCC
TTSQAGQTMIATAVQDNMRVISVILHADQSDTDERARFVETNRLLDYCFNSYTLEKLVAQ
CCCCCCCHHHHHHHHCCHHEEHHEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
NQVLPERLIVKQAAKQRLRVKANQSLMVVRPKDQTQLSYSLRIKDKVLKTPIRQNKKVGT
CCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEEEEEEEHHHHHHCHHHCCCCCCE
LIYQDPKLIDSGYLAAPPAVAIVSAKSIPEKGKLSKLFDFFQKVIGILINP
EEECCCCEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLIVTLAALMCLITNTSLAEDFSVNSRQAIAVEMTTGKILYAKEAQQKAPIASLTKLL
CHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCEEEEECCHHHHCCHHHHHHHH
TVYLVLKEIKAGRLQWDSQVILSEYALQLATDPDISNPAFEQRPYTIKELVESSLIISAN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCEEEECC
SSAVALAEHIAGSEPTFVDMMKKQLEAWGIRDYRLVNASGLNNSMLKGHLYPGSQPDDEN
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCC
QMTARDLALIAQQLLQDFPEVLTISSQAQLKWGEKDLPNSNLLLAGREMARTGVDGLKTG
HHHHHHHHHHHHHHHHHHHHHEECCCCHHCCCCCCCCCCCCEEEECHHHHHCCCCCCCCC
TTSQAGQTMIATAVQDNMRVISVILHADQSDTDERARFVETNRLLDYCFNSYTLEKLVAQ
CCCCCCCHHHHHHHHCCHHEEHHEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
NQVLPERLIVKQAAKQRLRVKANQSLMVVRPKDQTQLSYSLRIKDKVLKTPIRQNKKVGT
CCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEEEEEEEHHHHHHCHHHCCCCCCE
LIYQDPKLIDSGYLAAPPAVAIVSAKSIPEKGKLSKLFDFFQKVIGILINP
EEECCCCEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]