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The map label for this gene is dacA.1 [H]

Identifier: 195978796

GI number: 195978796

Start: 1746689

End: 1747975

Strand: Direct

Name: dacA.1 [H]

Synonym: Sez_1698

Alternate gene names: 195978796

Gene position: 1746689-1747975 (Clockwise)

Preceding gene: 195978772

Following gene: 195978797

Centisome position: 86.29

GC content: 40.09

Gene sequence:

>1287_bases
TTGAAAAATTATGGTATAATTAAAAAAACGACTAGAAGGATTATTCAAATGAAAAAGGTTTTAATAGCTTTGACCATATT
AGCCATATCTTGTCTGGCCTATCCAGCACAGGCTGATCATTATGATATTAGCGCCAAGCATGCGATTGCTGTTGAGCTTG
ATAGCGGTAAGATTCTATACGAAAAGGACGCCGACAAGACTGTTTCTGTGGCTTCTCTAAGCAAGGTTTTAACAGCTTAT
TTGGTGTACAAAGAGGTGCAAGCAGGAAAGTTAAAATGGGATAGCTCCGTAACGATCTCTAACTACCCTTACGAACTAAC
CACCAACTACACCATAAGCAATGTGCCCTTAGATGCTAGAAAATATACTGTTAAAGAGCTGTTAAAGGCTCTTATTGTCA
CCAATGCTAATAGCCCTGCTATTGCCCTGGCAGAAAAAATTGGAGGGACAGAGGCTAAGTTTGTTGATAAAATGATCGCT
CAATTGAAAGACTGGGGCATTACAGGTGCCAAGGTCGTTAATGCCAGTGGTCTGCCCAACCACATACTAGGAGAGCATAT
CTATCCGGGATCTAGCCTTGATGATGATAATCAATTTAGTGCAGCCGATCTTGCTCTTGTCACTAGCCATCTTTTAAAGG
AATTTCCTGAGGTTTTAAAGCTATCAAGTCAGGTTCAAGCTGAGTTTAATCATCAACAGATTTTTTCATACAACTATATG
CTAAAGGGAATGCCTAACTATCGTGATGGTGTTGATGGGCTTTTTGTTAGCTATTCTGAAAAGGGAGGCTCTTCTTTTTT
GGCTACTAGTGTCGAAAACAATATGCGTATTATTACAGTTGTCCTCAATGCTGACCATGCCCAAGACAACGATACGGCTG
TCTTTACAGCGACAAACCAATTGCTGCAGTATGTTTTAACGCATTTTAAAAAGGTTGTTGTCCTTGAAAAGGGTAAACCA
GATCAACTTAAAGCGCTTCCTGTTTTAGATAGTCCCCAAAAGAAAGTGCAGCTGGTGCCAAAAAAGAAGCTCACGATGAT
AAAGGCTCTTGACGACAAGTCTAAAACGACTTTTCAAATAAGCGATACAAAAAATCCCTTAGTAGCGCCTATCCAAAAAG
GACAGACTCTCGCAAAGGCCACACTTAATGATCATAGCTTAATCGGAAAAGGCTATCTAGGTCAAGCGCCAAGCGTTGAG
CTGGTGGCTCAAAAAAAGGTGCCAAAAAGCTTCTTTTTAAAGGTCTGGTGGAATCATTTTGTTCGTTATGTTAACCAATA
TCTCTAA

Upstream 100 bases:

>100_bases
ATACATTATTTGCTATTTTTATACAGTTCGTTATCGCTATCATAACAGCCAGCATTTTCTCATAATGATCTCTCTTCTCT
GAGAGGTTTAGTCAAGCTCC

Downstream 100 bases:

>100_bases
TGACTAGCTTTTATGCCTTATGTCCTCATTAGTGCCAAGGCCTCACTCAGGGCTTCATTAGGCTTATCTCGCAAAAATTG
ACCATTGTGTCAAGCTCTCC

Product: D-alanyl-D-alanine carboxypeptidase precursor DacA.1

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKNYGIIKKTTRRIIQMKKVLIALTILAISCLAYPAQADHYDISAKHAIAVELDSGKILYEKDADKTVSVASLSKVLTAY
LVYKEVQAGKLKWDSSVTISNYPYELTTNYTISNVPLDARKYTVKELLKALIVTNANSPAIALAEKIGGTEAKFVDKMIA
QLKDWGITGAKVVNASGLPNHILGEHIYPGSSLDDDNQFSAADLALVTSHLLKEFPEVLKLSSQVQAEFNHQQIFSYNYM
LKGMPNYRDGVDGLFVSYSEKGGSSFLATSVENNMRIITVVLNADHAQDNDTAVFTATNQLLQYVLTHFKKVVVLEKGKP
DQLKALPVLDSPQKKVQLVPKKKLTMIKALDDKSKTTFQISDTKNPLVAPIQKGQTLAKATLNDHSLIGKGYLGQAPSVE
LVAQKKVPKSFFLKVWWNHFVRYVNQYL

Sequences:

>Translated_428_residues
MKNYGIIKKTTRRIIQMKKVLIALTILAISCLAYPAQADHYDISAKHAIAVELDSGKILYEKDADKTVSVASLSKVLTAY
LVYKEVQAGKLKWDSSVTISNYPYELTTNYTISNVPLDARKYTVKELLKALIVTNANSPAIALAEKIGGTEAKFVDKMIA
QLKDWGITGAKVVNASGLPNHILGEHIYPGSSLDDDNQFSAADLALVTSHLLKEFPEVLKLSSQVQAEFNHQQIFSYNYM
LKGMPNYRDGVDGLFVSYSEKGGSSFLATSVENNMRIITVVLNADHAQDNDTAVFTATNQLLQYVLTHFKKVVVLEKGKP
DQLKALPVLDSPQKKVQLVPKKKLTMIKALDDKSKTTFQISDTKNPLVAPIQKGQTLAKATLNDHSLIGKGYLGQAPSVE
LVAQKKVPKSFFLKVWWNHFVRYVNQYL
>Mature_428_residues
MKNYGIIKKTTRRIIQMKKVLIALTILAISCLAYPAQADHYDISAKHAIAVELDSGKILYEKDADKTVSVASLSKVLTAY
LVYKEVQAGKLKWDSSVTISNYPYELTTNYTISNVPLDARKYTVKELLKALIVTNANSPAIALAEKIGGTEAKFVDKMIA
QLKDWGITGAKVVNASGLPNHILGEHIYPGSSLDDDNQFSAADLALVTSHLLKEFPEVLKLSSQVQAEFNHQQIFSYNYM
LKGMPNYRDGVDGLFVSYSEKGGSSFLATSVENNMRIITVVLNADHAQDNDTAVFTATNQLLQYVLTHFKKVVVLEKGKP
DQLKALPVLDSPQKKVQLVPKKKLTMIKALDDKSKTTFQISDTKNPLVAPIQKGQTLAKATLNDHSLIGKGYLGQAPSVE
LVAQKKVPKSFFLKVWWNHFVRYVNQYL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=415, Percent_Identity=26.0240963855422, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787062, Length=409, Percent_Identity=25.4278728606357, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082022, Length=359, Percent_Identity=23.9554317548747, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47467; Mature: 47467

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYGIIKKTTRRIIQMKKVLIALTILAISCLAYPAQADHYDISAKHAIAVELDSGKILY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEE
EKDADKTVSVASLSKVLTAYLVYKEVQAGKLKWDSSVTISNYPYELTTNYTISNVPLDAR
ECCCCCEEHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCEEEEECEEEECCCCCHH
KYTVKELLKALIVTNANSPAIALAEKIGGTEAKFVDKMIAQLKDWGITGAKVVNASGLPN
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
HILGEHIYPGSSLDDDNQFSAADLALVTSHLLKEFPEVLKLSSQVQAEFNHQQIFSYNYM
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEE
LKGMPNYRDGVDGLFVSYSEKGGSSFLATSVENNMRIITVVLNADHAQDNDTAVFTATNQ
ECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCEEEEEHHH
LLQYVLTHFKKVVVLEKGKPDQLKALPVLDSPQKKVQLVPKKKLTMIKALDDKSKTTFQI
HHHHHHHHHHEEEEEECCCCCCEEEECCCCCCHHHEEECCCHHHHHHHHHCCCCCEEEEE
SDTKNPLVAPIQKGQTLAKATLNDHSLIGKGYLGQAPSVELVAQKKVPKSFFLKVWWNHF
CCCCCCEECCCCCCCEEHHHHCCCCCEECCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHH
VRYVNQYL
HHHHHHCC
>Mature Secondary Structure
MKNYGIIKKTTRRIIQMKKVLIALTILAISCLAYPAQADHYDISAKHAIAVELDSGKILY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEE
EKDADKTVSVASLSKVLTAYLVYKEVQAGKLKWDSSVTISNYPYELTTNYTISNVPLDAR
ECCCCCEEHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCEEEEECEEEECCCCCHH
KYTVKELLKALIVTNANSPAIALAEKIGGTEAKFVDKMIAQLKDWGITGAKVVNASGLPN
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
HILGEHIYPGSSLDDDNQFSAADLALVTSHLLKEFPEVLKLSSQVQAEFNHQQIFSYNYM
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEE
LKGMPNYRDGVDGLFVSYSEKGGSSFLATSVENNMRIITVVLNADHAQDNDTAVFTATNQ
ECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCEEEEEHHH
LLQYVLTHFKKVVVLEKGKPDQLKALPVLDSPQKKVQLVPKKKLTMIKALDDKSKTTFQI
HHHHHHHHHHEEEEEECCCCCCEEEECCCCCCHHHEEECCCHHHHHHHHHCCCCCEEEEE
SDTKNPLVAPIQKGQTLAKATLNDHSLIGKGYLGQAPSVELVAQKKVPKSFFLKVWWNHF
CCCCCCEECCCCCCCEEHHHHCCCCCEECCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHH
VRYVNQYL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]