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The map label for this gene is dacA.3 [H]

Identifier: 195978798

GI number: 195978798

Start: 1749618

End: 1750799

Strand: Direct

Name: dacA.3 [H]

Synonym: Sez_1700

Alternate gene names: 195978798

Gene position: 1749618-1750799 (Clockwise)

Preceding gene: 195978797

Following gene: 195978808

Centisome position: 86.44

GC content: 40.95

Gene sequence:

>1182_bases
TTGACTAAAAGATTGCTACTATTCGTTTTGGTGTGCCTGTGCTTTCTAGGCATTTCTCAGGTTCACGCCGAAGATGTCAT
GGAAATTACCCGCAAGGCTGGCTATACCGTTAGTGAGGTTAATAGGCCAAAAGCCTCCATTGTTGTTGACGCAAAGACCT
CAGAGATCTTATGGCAGGATAACATTGACATGCCTAGAGACCCTGCTAGTATGTCAAAGATGTTTACGCTTTATATCCTC
TTTGAGGAATTGGCCAAAGGAAAGGTAACACTTGATACCACCATTACAGCAACAGAGACTGATCAGGCGATTAGTAAAAT
TTACGAAATCAGCAATAATAATATTGTTGCAGGAGTTGCGTACCCTATTCGTGATTTAATCATTATGACAGTGGTTCCAT
CATCAAATGCTGCAACAGTGATGATTGCCAACTACCTATCCAACAACGATGCGTCAGCCTTTATTGATCGGATCAACAAA
ACAGCCAAAGAGCTCGGCATGACTAATACGCATTTTTCAAATGCTAGCGGAGCAATCGCACAAGCCTTTCAAGGCTACTA
TCTCCCAGAAAAATATGATTTAACAGCTTCTAATATCACAACAGCACGTGATTTTGCAACCTTAATTTATTATTTTTTGA
CCAATTATCCTAGTATTCTTGATTTTACCAATCAAACTGTCGTCCGCACCATGGTTGGAACCCCTTATGAGGAAGAGTTC
CATTCCTACAACCACTCTCTGCCTGATGGTAAGTTTGGAATAGAGGGTGTTGATGGTATGAAAACAGGATCCAGCCCAAG
TGCTGCTTTCAATGCGGTCGTAACAGCAAAACGAGGAAATACCAGACTGATTACAGTTGTCCTAGGAGTTGGTGATTGGT
CTGACCAAAATGGAGAATTTTACCGTCACCCATTCATTAATGCTTTGACTGAAAAAGCCTTTAAAGATAGTAAAACACTC
ACCAAAGCAAAACGAAGCAAGCTTAAAAGGCTTGTTGCTAAACCAGCCAGCACAGCAGCTGCTGCTAAAAGGCAGTTGCC
ACAAGCCACAACTGATCAATCATTTCTTGAAAAAATAGAACAATTTGTCGATGATCATCGTCCCCTTGTCCTAATTTGCT
TAGCCATTTTTATCCTTATTGTTTTACTACTTGCTCTGATGGCCTTTATGATGGGACGTTAA

Upstream 100 bases:

>100_bases
TTCTCATTAACCCTTAATATTTTCAAAAAATATATTTCATTTGTATTATTGAAATGATATAATAAGCTTACATTATTTTT
TTACATTAAGGAGCTTTGAA

Downstream 100 bases:

>100_bases
GCTCATTGCTGATTAAAAAGACTTCTTGGTAGCTGCCAAGAAGTCTTTTTTATGCCTTAGGTATTTAAAAACTAACCTCT
TGGGTTAGTACCTATGTCTT

Product: D-alanyl-D-alanine carboxypeptidase precursor DacA.3

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTKRLLLFVLVCLCFLGISQVHAEDVMEITRKAGYTVSEVNRPKASIVVDAKTSEILWQDNIDMPRDPASMSKMFTLYIL
FEELAKGKVTLDTTITATETDQAISKIYEISNNNIVAGVAYPIRDLIIMTVVPSSNAATVMIANYLSNNDASAFIDRINK
TAKELGMTNTHFSNASGAIAQAFQGYYLPEKYDLTASNITTARDFATLIYYFLTNYPSILDFTNQTVVRTMVGTPYEEEF
HSYNHSLPDGKFGIEGVDGMKTGSSPSAAFNAVVTAKRGNTRLITVVLGVGDWSDQNGEFYRHPFINALTEKAFKDSKTL
TKAKRSKLKRLVAKPASTAAAAKRQLPQATTDQSFLEKIEQFVDDHRPLVLICLAIFILIVLLLALMAFMMGR

Sequences:

>Translated_393_residues
MTKRLLLFVLVCLCFLGISQVHAEDVMEITRKAGYTVSEVNRPKASIVVDAKTSEILWQDNIDMPRDPASMSKMFTLYIL
FEELAKGKVTLDTTITATETDQAISKIYEISNNNIVAGVAYPIRDLIIMTVVPSSNAATVMIANYLSNNDASAFIDRINK
TAKELGMTNTHFSNASGAIAQAFQGYYLPEKYDLTASNITTARDFATLIYYFLTNYPSILDFTNQTVVRTMVGTPYEEEF
HSYNHSLPDGKFGIEGVDGMKTGSSPSAAFNAVVTAKRGNTRLITVVLGVGDWSDQNGEFYRHPFINALTEKAFKDSKTL
TKAKRSKLKRLVAKPASTAAAAKRQLPQATTDQSFLEKIEQFVDDHRPLVLICLAIFILIVLLLALMAFMMGR
>Mature_392_residues
TKRLLLFVLVCLCFLGISQVHAEDVMEITRKAGYTVSEVNRPKASIVVDAKTSEILWQDNIDMPRDPASMSKMFTLYILF
EELAKGKVTLDTTITATETDQAISKIYEISNNNIVAGVAYPIRDLIIMTVVPSSNAATVMIANYLSNNDASAFIDRINKT
AKELGMTNTHFSNASGAIAQAFQGYYLPEKYDLTASNITTARDFATLIYYFLTNYPSILDFTNQTVVRTMVGTPYEEEFH
SYNHSLPDGKFGIEGVDGMKTGSSPSAAFNAVVTAKRGNTRLITVVLGVGDWSDQNGEFYRHPFINALTEKAFKDSKTLT
KAKRSKLKRLVAKPASTAAAAKRQLPQATTDQSFLEKIEQFVDDHRPLVLICLAIFILIVLLLALMAFMMGR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=296, Percent_Identity=28.0405405405405, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787062, Length=252, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082022, Length=250, Percent_Identity=25.2, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43529; Mature: 43397

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLLLFVLVCLCFLGISQVHAEDVMEITRKAGYTVSEVNRPKASIVVDAKTSEILWQD
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCEEECC
NIDMPRDPASMSKMFTLYILFEELAKGKVTLDTTITATETDQAISKIYEISNNNIVAGVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHCCCCEEEEEH
YPIRDLIIMTVVPSSNAATVMIANYLSNNDASAFIDRINKTAKELGMTNTHFSNASGAIA
HHHHCEEEEEEECCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QAFQGYYLPEKYDLTASNITTARDFATLIYYFLTNYPSILDFTNQTVVRTMVGTPYEEEF
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCHHHHH
HSYNHSLPDGKFGIEGVDGMKTGSSPSAAFNAVVTAKRGNTRLITVVLGVGDWSDQNGEF
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEEEECCCCCCCCCCE
YRHPFINALTEKAFKDSKTLTKAKRSKLKRLVAKPASTAAAAKRQLPQATTDQSFLEKIE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
QFVDDHRPLVLICLAIFILIVLLLALMAFMMGR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKRLLLFVLVCLCFLGISQVHAEDVMEITRKAGYTVSEVNRPKASIVVDAKTSEILWQD
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEECCCCCEEECC
NIDMPRDPASMSKMFTLYILFEELAKGKVTLDTTITATETDQAISKIYEISNNNIVAGVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHCCCCEEEEEH
YPIRDLIIMTVVPSSNAATVMIANYLSNNDASAFIDRINKTAKELGMTNTHFSNASGAIA
HHHHCEEEEEEECCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QAFQGYYLPEKYDLTASNITTARDFATLIYYFLTNYPSILDFTNQTVVRTMVGTPYEEEF
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCHHHHH
HSYNHSLPDGKFGIEGVDGMKTGSSPSAAFNAVVTAKRGNTRLITVVLGVGDWSDQNGEF
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEEEECCCCCCCCCCE
YRHPFINALTEKAFKDSKTLTKAKRSKLKRLVAKPASTAAAAKRQLPQATTDQSFLEKIE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
QFVDDHRPLVLICLAIFILIVLLLALMAFMMGR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]