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The map label for this gene is nrdE [H]

Identifier: 195977667

GI number: 195977667

Start: 549381

End: 551555

Strand: Direct

Name: nrdE [H]

Synonym: Sez_0526

Alternate gene names: 195977667

Gene position: 549381-551555 (Clockwise)

Preceding gene: 195977666

Following gene: 195977669

Centisome position: 27.14

GC content: 45.66

Gene sequence:

>2175_bases
ATGTCACAAAAAGAGTCCTACCTCTCATTAAATGCCCTTACACGCTTTAAACGAGCAGATGGTACCTATCACTTTGACAG
TGATAAGGAAGCTGTTCAGCGCTATTTAGAGGAGCATGTCTACCCACAAAGCCTATCCTTTGATAGTTTGGAGGAGAAAT
GGCGTTATTTGGTGGCGCATGATTATTATGATCAAGAGATTTTAAAGGCTTATTCGGCTGAATGTATCACTGAAGCCTTT
ACCTACGCTTACCAGCAAAAGTTCTCATTTTTGAGCTTAATGGGAGCCATGAAATTTTACCAGTCCTATGCGCTTAAAAC
TCTAGATGGGTCACAATACCTAGAGCATTTTGAGGATCGTGCTGTCATGAATGCCTTGTTTTTGGCTAATGGTGATCCTG
ATTTGGTGCGGGATTTGATTGATCAGATACTTAGCAGGCGCTTTCAACCGGCGACCCCTACCTTTTTAAATGCAGGTAAA
AAGCGTCGAGGCGAATATATTTCCTGTTATTTGCTGCGAATTGAAGACAACATGGAGTCTATTTCGCGTGCGATTGGCAC
GAGCTTGCAGCTCTCAAAAAGAGGCGGCGGCGTTGCTTTGTGTTTAACCAATCTAAGAGAGGTTGGCTCTCCCATAAAAG
GCATTGAAAATCAAGCGACAGGCATTGTGCCAGTGATGAAGCTACTGGAGGATTCCTTTTCCTATGCCAATCAGCTAGGG
CAAAGACAGGGAGCAGGTGCGGTTTACCTCAATGTGCATCACCCAGAGATTCTGACCTTTTTAGATACGAAGAGAGAAAA
TGCAGACGAAAAAATCCGTATCAAGTCACTGGCGCTGGGTGTCGTTATTCCTGATATTACCTTTCAGCTGGCACGGGAAA
ATAAGGACATGGCGCTATTTTCTCCCTATGACATTAAGCGAGTTTATGGCAAGGACATGTCAGATATTGCCATTACCGAG
GAGTATGACCGCCTAGTGGCCAACCCAGCCATTAAAAAGACCTATATCAGTGCCCGTAAGCTCTTTCAGCTTATTGCAGA
GCTTCATTTTGAATCTGGCTACCCTTATCTCTTGTTTGATGATACGGTTAATAAGCGCAATCCACACCCACAAAAGGGGC
GGATTGTCATGTCAAACCTTTGCTCTGAAATCGCTCAGGTCAGCACAGCCAGCACCTTTGAAGAGGATTTGACCTTTAAG
CAAATCGGCGAGGACATTTGCTGTAACCTTGGCTCCATTAATATTGCCGAAGCCATGAAGCATGCGGCTGGCTTTGAGCA
ATTGATTGCAACCAGCATTAGGGCCTTGGATCATGTGTCAAGAGCTTCAGACGTCAGCTGTGCTCCTTCTGTTGAAAAGG
GGAATGCTGCTAATCATGCTGTTGGTCTTGGGGCGATGAACCTGCATGGTTTTTTGGCGACCAATCACATCTTTTATGAT
TCAGCTGAAGCAGTAGATTTTACTGATCTCTTTTTCCACACAATGGCCTATTATGCCTTTAAGGCTTCTAGTCAATTGGC
TAAGGAAAAGGGAGCATTTGCTGGCTTTGAGCAGTCAACCTATGCAGATGGCAGCTACTTTGCCCGCTATTTGACAAAAG
AGGCTAAGCCTAAGACAGCTAAGGTTGCGGCCTTGCTTGAGCGCTACGGGTTTCGAGTGCCTAGTGTGGCTGATTGGCAG
GAATTAGTTGCGACGATTCAAGAGACAGGCCTTGCCAATGCTCACTTGATGGCAGTTGCACCAACCGGCTCGATCAGCTA
CTTGTCCTCATGTACGCCGAGCTTACAGCCTGTTGTTGCACCGGTTGAGGTGCGAAAGGAGGGGCACTTAGGAAGGATTT
ATGTGCCTGCTTATCAGATAAGCGATGATAACTATGCTTATTATGAGCGTGGTGCCTATGAGGTTGGGCCTGAGCCTATT
ATTAATATTGTAGCGGCGGCTCAAAAGCACGTTGATCAGGCAATTTCCTTAACCCTATTTATGACAGACCAAGCAACAAC
ACGTGATCTCAATCGTGCCTATATACACGCCTTTAAGTTAAAGTGTGCGTCCATTTATTATGTCCGCGTCAGACAGGACA
TTCTAGAAGGAAGTGAGCAGTATGAGGAAGATACGATTGTAGCGCTTACCGATACCAATGCTTCAGGCGAGGCATGCCAA
TCCTGCATGCTTTAG

Upstream 100 bases:

>100_bases
AAGAGATGTCCTCAAGGTGCAGGCGATTTTAGCAGGTGATTGGCTGTCATACTGATTATTACCGTAGAATGATCACACAT
TGTAACGAAAGGAAAAAAGA

Downstream 100 bases:

>100_bases
ATCTTTTTTAGCCTTAAAAATAGGCAGCACCAAGTAAGCCTTCATCCAGCATGAAGGAATTGGCTCACCTAAGCTCTGTC
TAGCCAGTTTGTTAATAGGC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MSQKESYLSLNALTRFKRADGTYHFDSDKEAVQRYLEEHVYPQSLSFDSLEEKWRYLVAHDYYDQEILKAYSAECITEAF
TYAYQQKFSFLSLMGAMKFYQSYALKTLDGSQYLEHFEDRAVMNALFLANGDPDLVRDLIDQILSRRFQPATPTFLNAGK
KRRGEYISCYLLRIEDNMESISRAIGTSLQLSKRGGGVALCLTNLREVGSPIKGIENQATGIVPVMKLLEDSFSYANQLG
QRQGAGAVYLNVHHPEILTFLDTKRENADEKIRIKSLALGVVIPDITFQLARENKDMALFSPYDIKRVYGKDMSDIAITE
EYDRLVANPAIKKTYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHPQKGRIVMSNLCSEIAQVSTASTFEEDLTFK
QIGEDICCNLGSINIAEAMKHAAGFEQLIATSIRALDHVSRASDVSCAPSVEKGNAANHAVGLGAMNLHGFLATNHIFYD
SAEAVDFTDLFFHTMAYYAFKASSQLAKEKGAFAGFEQSTYADGSYFARYLTKEAKPKTAKVAALLERYGFRVPSVADWQ
ELVATIQETGLANAHLMAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGHLGRIYVPAYQISDDNYAYYERGAYEVGPEPI
INIVAAAQKHVDQAISLTLFMTDQATTRDLNRAYIHAFKLKCASIYYVRVRQDILEGSEQYEEDTIVALTDTNASGEACQ
SCML

Sequences:

>Translated_724_residues
MSQKESYLSLNALTRFKRADGTYHFDSDKEAVQRYLEEHVYPQSLSFDSLEEKWRYLVAHDYYDQEILKAYSAECITEAF
TYAYQQKFSFLSLMGAMKFYQSYALKTLDGSQYLEHFEDRAVMNALFLANGDPDLVRDLIDQILSRRFQPATPTFLNAGK
KRRGEYISCYLLRIEDNMESISRAIGTSLQLSKRGGGVALCLTNLREVGSPIKGIENQATGIVPVMKLLEDSFSYANQLG
QRQGAGAVYLNVHHPEILTFLDTKRENADEKIRIKSLALGVVIPDITFQLARENKDMALFSPYDIKRVYGKDMSDIAITE
EYDRLVANPAIKKTYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHPQKGRIVMSNLCSEIAQVSTASTFEEDLTFK
QIGEDICCNLGSINIAEAMKHAAGFEQLIATSIRALDHVSRASDVSCAPSVEKGNAANHAVGLGAMNLHGFLATNHIFYD
SAEAVDFTDLFFHTMAYYAFKASSQLAKEKGAFAGFEQSTYADGSYFARYLTKEAKPKTAKVAALLERYGFRVPSVADWQ
ELVATIQETGLANAHLMAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGHLGRIYVPAYQISDDNYAYYERGAYEVGPEPI
INIVAAAQKHVDQAISLTLFMTDQATTRDLNRAYIHAFKLKCASIYYVRVRQDILEGSEQYEEDTIVALTDTNASGEACQ
SCML
>Mature_723_residues
SQKESYLSLNALTRFKRADGTYHFDSDKEAVQRYLEEHVYPQSLSFDSLEEKWRYLVAHDYYDQEILKAYSAECITEAFT
YAYQQKFSFLSLMGAMKFYQSYALKTLDGSQYLEHFEDRAVMNALFLANGDPDLVRDLIDQILSRRFQPATPTFLNAGKK
RRGEYISCYLLRIEDNMESISRAIGTSLQLSKRGGGVALCLTNLREVGSPIKGIENQATGIVPVMKLLEDSFSYANQLGQ
RQGAGAVYLNVHHPEILTFLDTKRENADEKIRIKSLALGVVIPDITFQLARENKDMALFSPYDIKRVYGKDMSDIAITEE
YDRLVANPAIKKTYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHPQKGRIVMSNLCSEIAQVSTASTFEEDLTFKQ
IGEDICCNLGSINIAEAMKHAAGFEQLIATSIRALDHVSRASDVSCAPSVEKGNAANHAVGLGAMNLHGFLATNHIFYDS
AEAVDFTDLFFHTMAYYAFKASSQLAKEKGAFAGFEQSTYADGSYFARYLTKEAKPKTAKVAALLERYGFRVPSVADWQE
LVATIQETGLANAHLMAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGHLGRIYVPAYQISDDNYAYYERGAYEVGPEPII
NIVAAAQKHVDQAISLTLFMTDQATTRDLNRAYIHAFKLKCASIYYVRVRQDILEGSEQYEEDTIVALTDTNASGEACQS
CML

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=654, Percent_Identity=24.7706422018349, Blast_Score=181, Evalue=2e-45,
Organism=Escherichia coli, GI1789030, Length=719, Percent_Identity=53.8247566063978, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=588, Percent_Identity=26.7006802721088, Blast_Score=194, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17555242, Length=633, Percent_Identity=23.8546603475513, Blast_Score=167, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322123, Length=645, Percent_Identity=25.5813953488372, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320914, Length=653, Percent_Identity=25.114854517611, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17352481, Length=696, Percent_Identity=24.5689655172414, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81015; Mature: 80883

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKESYLSLNALTRFKRADGTYHFDSDKEAVQRYLEEHVYPQSLSFDSLEEKWRYLVAH
CCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHEEHHC
DYYDQEILKAYSAECITEAFTYAYQQKFSFLSLMGAMKFYQSYALKTLDGSQYLEHFEDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AVMNALFLANGDPDLVRDLIDQILSRRFQPATPTFLNAGKKRRGEYISCYLLRIEDNMES
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEEECCHHH
ISRAIGTSLQLSKRGGGVALCLTNLREVGSPIKGIENQATGIVPVMKLLEDSFSYANQLG
HHHHHCCCEEEECCCCCEEEHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
QRQGAGAVYLNVHHPEILTFLDTKRENADEKIRIKSLALGVVIPDITFQLARENKDMALF
HCCCCCEEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEEEEECCHHHHHHHCCCCEEEE
SPYDIKRVYGKDMSDIAITEEYDRLVANPAIKKTYISARKLFQLIAELHFESGYPYLLFD
CCCHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
DTVNKRNPHPQKGRIVMSNLCSEIAQVSTASTFEEDLTFKQIGEDICCNLGSINIAEAMK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCHHHHHH
HAAGFEQLIATSIRALDHVSRASDVSCAPSVEKGNAANHAVGLGAMNLHGFLATNHIFYD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHEEEECEEEEC
SAEAVDFTDLFFHTMAYYAFKASSQLAKEKGAFAGFEQSTYADGSYFARYLTKEAKPKTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
KVAALLERYGFRVPSVADWQELVATIQETGLANAHLMAVAPTGSISYLSSCTPSLQPVVA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCC
PVEVRKEGHLGRIYVPAYQISDDNYAYYERGAYEVGPEPIINIVAAAQKHVDQAISLTLF
CHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEE
MTDQATTRDLNRAYIHAFKLKCASIYYVRVRQDILEGSEQYEEDTIVALTDTNASGEACQ
EECCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHHHH
SCML
HCCC
>Mature Secondary Structure 
SQKESYLSLNALTRFKRADGTYHFDSDKEAVQRYLEEHVYPQSLSFDSLEEKWRYLVAH
CCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHEEHHC
DYYDQEILKAYSAECITEAFTYAYQQKFSFLSLMGAMKFYQSYALKTLDGSQYLEHFEDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AVMNALFLANGDPDLVRDLIDQILSRRFQPATPTFLNAGKKRRGEYISCYLLRIEDNMES
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEEECCHHH
ISRAIGTSLQLSKRGGGVALCLTNLREVGSPIKGIENQATGIVPVMKLLEDSFSYANQLG
HHHHHCCCEEEECCCCCEEEHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
QRQGAGAVYLNVHHPEILTFLDTKRENADEKIRIKSLALGVVIPDITFQLARENKDMALF
HCCCCCEEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEEEEECCHHHHHHHCCCCEEEE
SPYDIKRVYGKDMSDIAITEEYDRLVANPAIKKTYISARKLFQLIAELHFESGYPYLLFD
CCCHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
DTVNKRNPHPQKGRIVMSNLCSEIAQVSTASTFEEDLTFKQIGEDICCNLGSINIAEAMK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCHHHHHH
HAAGFEQLIATSIRALDHVSRASDVSCAPSVEKGNAANHAVGLGAMNLHGFLATNHIFYD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHEEEECEEEEC
SAEAVDFTDLFFHTMAYYAFKASSQLAKEKGAFAGFEQSTYADGSYFARYLTKEAKPKTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
KVAALLERYGFRVPSVADWQELVATIQETGLANAHLMAVAPTGSISYLSSCTPSLQPVVA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCC
PVEVRKEGHLGRIYVPAYQISDDNYAYYERGAYEVGPEPIINIVAAAQKHVDQAISLTLF
CHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEE
MTDQATTRDLNRAYIHAFKLKCASIYYVRVRQDILEGSEQYEEDTIVALTDTNASGEACQ
EECCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHHHH
SCML
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]