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The map label for this gene is braB [C]

Identifier: 195977669

GI number: 195977669

Start: 552109

End: 553452

Strand: Direct

Name: braB [C]

Synonym: Sez_0528

Alternate gene names: 195977669

Gene position: 552109-553452 (Clockwise)

Preceding gene: 195977667

Following gene: 195977671

Centisome position: 27.28

GC content: 43.75

Gene sequence:

>1344_bases
ATGGTTAGAAAAGGATTTTTAACGGGCTTACTTTTATTTGGTATCTTTTTTGGAGCAGGAAATTTGATTTTTCCACCGAC
CTTAGGTGTTGGCTCAGGTCAGGAATTTTGGTCTGCGATTTTGGGCTTTTGCCTATCTGGCGTAGGATTAGCGGTGATTA
CGCTTTTGCTAGGTACACTGACAAATGGTGGTTACAGAGAAGAAATGACTCAAAAATTTGGATCATGGTTTTCGATAACC
TTTCTCGTTGTGCTTTATTTGACGATTGGTCCTCTTTTTGCCATCCCAAGAACGGCAACGGTTTCCTTTGAGGTTGGGGT
CAGCCCACTTGTCGGCCATAGTCCACTTGCTCTTTTCTTGTTTTCAGCCTGCTTTTTTGCAGCTGCCTATTTTCTTGCTA
TTAGGCCAAACGGTATCCTAGATAGCGTTGGGAAAATTCTGACACCGGTCTTTGCCTTGCTTATTCTACTACTAGTGGTG
GTAGGAGCCATTGTTTACGGAGGGCAGGAGCCTGCTCAAGCAAGCGCAGCCTATGCAGATCAAGCTTTTGGCAGCGGTGT
TCTGGCTGGCTACAATACCTTAGATGCTCTGGCAGCTGTCGCTTTTTGCCTGGTAGCAACAGAAACGCTTAAACAGTTCG
GCTTTCAAAGCAAAAAGGAATACCTATCGACCATCTGGGTGGTAGGTATCGTCACCAGTCTTGCTTTTAGCATTCTTTAT
ATTGGACTTGGCTTTTTAGGCAATAGGTTTCCTATTCCAGCTGATGTTTTAGCCGATCCTAATGTGAATCAAGGTGCTTA
CGTGCTATCTCAGGCCTCTTATCAATTGTTTGGTTCCTTTGGCCGCTTTTTCCTTAGTGTGATGGTTATTTTAACCTGCT
TTACCACAACGGTTGGTCTGATTGTCTCAGTTTCAGAGTTCTTTGAAAAGAGCTTTCGTTTTGGGAATTATAAGCTCTTT
GCAGGCGTGTTTACCTTTGTTGGCTTTTTGATTGCTAATCTTGGCTTAAATGCTGTTATTACCTTTTCTGTTCCGGTCTT
GACCCTGCTTTATCCGATTGTTATTGTCATGGTCGTTATTATTTTGCTCAACAAGCTAGTATCTCTCTCTAAAAAGGGCA
TGACATTAACTATTGGCTTAGTGGCCTTAGTCTCCTTGATGGAGGTCTTGGCAAGCCAATTTCAGCTGGGAGCTTTGACA
GCACTGGTAGCATTACTGCCTTTTCACACAGTGTCTATGGGCTGGCTCGTCCCAGGTCTAGTCGGCTTGCTTGTGGCTCT
GCTTTTGCCAGATAAGCAAAAAGGTCAAGCCTTTGATCTAGAAGCCTTTAATCATCAGGATTAG

Upstream 100 bases:

>100_bases
GAGAGACAAGGGTTTGGCTGTGTCTATCTTAGCTTAACTGAAGTGCTAGTATTTTGTACAGAAAGGGTGTAGGCTTTCCA
GCTTACATAAGTGGTTGCCG

Downstream 100 bases:

>100_bases
TGCCTATCATCGCTATAGCACAAGCTATTTAAGCGGTGTTCTTACCTTAAAGATCCTCACTCAATGACCTTGAGCGGGGT
CTTTTGGCTCTTTGTCAACT

Product: branched-chain amino acid transport system carrier protein BraB

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MVRKGFLTGLLLFGIFFGAGNLIFPPTLGVGSGQEFWSAILGFCLSGVGLAVITLLLGTLTNGGYREEMTQKFGSWFSIT
FLVVLYLTIGPLFAIPRTATVSFEVGVSPLVGHSPLALFLFSACFFAAAYFLAIRPNGILDSVGKILTPVFALLILLLVV
VGAIVYGGQEPAQASAAYADQAFGSGVLAGYNTLDALAAVAFCLVATETLKQFGFQSKKEYLSTIWVVGIVTSLAFSILY
IGLGFLGNRFPIPADVLADPNVNQGAYVLSQASYQLFGSFGRFFLSVMVILTCFTTTVGLIVSVSEFFEKSFRFGNYKLF
AGVFTFVGFLIANLGLNAVITFSVPVLTLLYPIVIVMVVIILLNKLVSLSKKGMTLTIGLVALVSLMEVLASQFQLGALT
ALVALLPFHTVSMGWLVPGLVGLLVALLLPDKQKGQAFDLEAFNHQD

Sequences:

>Translated_447_residues
MVRKGFLTGLLLFGIFFGAGNLIFPPTLGVGSGQEFWSAILGFCLSGVGLAVITLLLGTLTNGGYREEMTQKFGSWFSIT
FLVVLYLTIGPLFAIPRTATVSFEVGVSPLVGHSPLALFLFSACFFAAAYFLAIRPNGILDSVGKILTPVFALLILLLVV
VGAIVYGGQEPAQASAAYADQAFGSGVLAGYNTLDALAAVAFCLVATETLKQFGFQSKKEYLSTIWVVGIVTSLAFSILY
IGLGFLGNRFPIPADVLADPNVNQGAYVLSQASYQLFGSFGRFFLSVMVILTCFTTTVGLIVSVSEFFEKSFRFGNYKLF
AGVFTFVGFLIANLGLNAVITFSVPVLTLLYPIVIVMVVIILLNKLVSLSKKGMTLTIGLVALVSLMEVLASQFQLGALT
ALVALLPFHTVSMGWLVPGLVGLLVALLLPDKQKGQAFDLEAFNHQD
>Mature_447_residues
MVRKGFLTGLLLFGIFFGAGNLIFPPTLGVGSGQEFWSAILGFCLSGVGLAVITLLLGTLTNGGYREEMTQKFGSWFSIT
FLVVLYLTIGPLFAIPRTATVSFEVGVSPLVGHSPLALFLFSACFFAAAYFLAIRPNGILDSVGKILTPVFALLILLLVV
VGAIVYGGQEPAQASAAYADQAFGSGVLAGYNTLDALAAVAFCLVATETLKQFGFQSKKEYLSTIWVVGIVTSLAFSILY
IGLGFLGNRFPIPADVLADPNVNQGAYVLSQASYQLFGSFGRFFLSVMVILTCFTTTVGLIVSVSEFFEKSFRFGNYKLF
AGVFTFVGFLIANLGLNAVITFSVPVLTLLYPIVIVMVVIILLNKLVSLSKKGMTLTIGLVALVSLMEVLASQFQLGALT
ALVALLPFHTVSMGWLVPGLVGLLVALLLPDKQKGQAFDLEAFNHQD

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential) [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=432, Percent_Identity=33.3333333333333, Blast_Score=210, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47703; Mature: 47703

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKGFLTGLLLFGIFFGAGNLIFPPTLGVGSGQEFWSAILGFCLSGVGLAVITLLLGTL
CCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNGGYREEMTQKFGSWFSITFLVVLYLTIGPLFAIPRTATVSFEVGVSPLVGHSPLALFL
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
FSACFFAAAYFLAIRPNGILDSVGKILTPVFALLILLLVVVGAIVYGGQEPAQASAAYAD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QAFGSGVLAGYNTLDALAAVAFCLVATETLKQFGFQSKKEYLSTIWVVGIVTSLAFSILY
HHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGLGFLGNRFPIPADVLADPNVNQGAYVLSQASYQLFGSFGRFFLSVMVILTCFTTTVGL
HHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVSVSEFFEKSFRFGNYKLFAGVFTFVGFLIANLGLNAVITFSVPVLTLLYPIVIVMVVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHH
ILLNKLVSLSKKGMTLTIGLVALVSLMEVLASQFQLGALTALVALLPFHTVSMGWLVPGL
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGLLVALLLPDKQKGQAFDLEAFNHQD
HHHHHHHHCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MVRKGFLTGLLLFGIFFGAGNLIFPPTLGVGSGQEFWSAILGFCLSGVGLAVITLLLGTL
CCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNGGYREEMTQKFGSWFSITFLVVLYLTIGPLFAIPRTATVSFEVGVSPLVGHSPLALFL
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
FSACFFAAAYFLAIRPNGILDSVGKILTPVFALLILLLVVVGAIVYGGQEPAQASAAYAD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QAFGSGVLAGYNTLDALAAVAFCLVATETLKQFGFQSKKEYLSTIWVVGIVTSLAFSILY
HHHCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGLGFLGNRFPIPADVLADPNVNQGAYVLSQASYQLFGSFGRFFLSVMVILTCFTTTVGL
HHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVSVSEFFEKSFRFGNYKLFAGVFTFVGFLIANLGLNAVITFSVPVLTLLYPIVIVMVVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHH
ILLNKLVSLSKKGMTLTIGLVALVSLMEVLASQFQLGALTALVALLPFHTVSMGWLVPGL
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGLLVALLLPDKQKGQAFDLEAFNHQD
HHHHHHHHCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA