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The map label for this gene is gatA [H]

Identifier: 195977533

GI number: 195977533

Start: 418183

End: 419649

Strand: Direct

Name: gatA [H]

Synonym: Sez_0390

Alternate gene names: 195977533

Gene position: 418183-419649 (Clockwise)

Preceding gene: 195977532

Following gene: 195977534

Centisome position: 20.66

GC content: 46.35

Gene sequence:

>1467_bases
ATGTCTTTTAATCATCACACTATAGAAGAATTACATGAGCTTCTTGTTGCAAAGGACATTTCTGCAGTTGAGCTAACAAA
GGCAACCCTTGAGGATATCAAGGCGCGTGAAGAGGCTGTGGGTTCTTTCATTACCATTGCTGAGGAGGCAGCCTTGAAGC
AGGCTGCGGCACTTGATGCCAAGGGGATTGATCCTGACAATGTGATGTCAGGAATACCACTAGCTGTCAAGGACAATATC
TCAACCAAGGGAATCCTGACAACTGCTGCCTCTAAGATGCTTTATAATTATGAGCCGATTTTTGATGCGACAGCAGTAGC
TAATGCCTATGATAAGGATATGATTATCATCGGTAAGACCAACATGGACGAGTTTGCTATGGGTGGCTCAACAGAAACGT
CTTATTTCAAGAAAACCAAAAACGCTTGGGATCGTAGCAGGGTTCCTGGTGGTTCATCAGGTGGCTCTGCAACAGCAGTA
GCTTCAGGTCAGGTTCGCTTATCCCTTGGCTCAGATACTGGAGGCTCTATTCGTCAGCCAGCAGCCTTTAATGGTGTTGT
CGGGCTGAAGCCAACCTACGGGGCTGTTTCTCGCTATGGCTTGATTGCCTTTGGCAGCTCGCTGGATCAGATAGGGCCTT
TTGCGCCAACGGTCAGAGAAAATGCTCAGCTGCTGACTGTCATAGCAGGGAGTGATCGTAAGGACTCAACCTCTGCACCT
GTGCAGATTGCTGACTATACCAGTAAAATTGGCCAAGATATCAAGGGCATGAAAATTGCACTACCTAAAGAATATCTAGG
CGAAGGCATTGATCCTAAGATTAAAGAGACGGTTTTAGCTGCTGCTAAGCATTTTGAGAAGCTGGGAGCTATCATTGAGG
AGGTCAGTCTGCCACATAGCAAGTATGGGGTGGCTGTCTATTATATCATTGCTTCATCTGAGGCCTCGTCAAACCTACAG
CGATTTGATGGTATTCGCTATGGCTTTCGTGCTGCTGATGCCAAGAGCCTTGAGGACATCTATGTTAAGACGCGTAGTCA
GGGCTTTGGTGATGAGGTTAAGCGTCGTATCATGCTTGGTACCTTTAGCTTATCGTCAGGCTATTACGATGCTTATTTCA
AAAAGGCAGGTCAGGTTCGGACGCTGATCATTCAAGATTTTGAGAAGGTTTTTGCTGATTATGACTTGATTTTAGGACCA
ACAGCACCAACAGCAGCCTTTGAGCTTGATACGTTGAATCATGATCCTGTTGCCATGTATTTGGCTGATCTTTTGACCAT
TCCGGTGAATCTAGCGGGCTTGCCTGCGATCTCTATTCCAGCAGGCTTTGCAGATGGCTTACCTGTTGGGCTTCAATTGA
TCGGTCCAAAATATAGTGAAGAGGTCATCTATCAGGCAGCAGCAGCCTTTGAAGCGACTACTGACTACCACAAGCAGCAG
CCTATGATTTTTGGAGGTGACAGCTAA

Upstream 100 bases:

>100_bases
AGGCTGGAGTGGATCGCAAGTTCCTGTTTCAAAATGTTCCTGAAAAAGAAAATCATTTCATCAAGGTACCAGCTATTCTT
GATGATGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
TGAATTTTGAAACCATTATTGGACTTGAGGTCCATGTGGAGTTGAATACCAATTCTAAGATTTTCTCGCCTTCATCAGCT
CATTTTGGAGAGGATCCCAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSFNHHTIEELHELLVAKDISAVELTKATLEDIKAREEAVGSFITIAEEAALKQAAALDAKGIDPDNVMSGIPLAVKDNI
STKGILTTAASKMLYNYEPIFDATAVANAYDKDMIIIGKTNMDEFAMGGSTETSYFKKTKNAWDRSRVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGAVSRYGLIAFGSSLDQIGPFAPTVRENAQLLTVIAGSDRKDSTSAP
VQIADYTSKIGQDIKGMKIALPKEYLGEGIDPKIKETVLAAAKHFEKLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRAADAKSLEDIYVKTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFADYDLILGP
TAPTAAFELDTLNHDPVAMYLADLLTIPVNLAGLPAISIPAGFADGLPVGLQLIGPKYSEEVIYQAAAAFEATTDYHKQQ
PMIFGGDS

Sequences:

>Translated_488_residues
MSFNHHTIEELHELLVAKDISAVELTKATLEDIKAREEAVGSFITIAEEAALKQAAALDAKGIDPDNVMSGIPLAVKDNI
STKGILTTAASKMLYNYEPIFDATAVANAYDKDMIIIGKTNMDEFAMGGSTETSYFKKTKNAWDRSRVPGGSSGGSATAV
ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGAVSRYGLIAFGSSLDQIGPFAPTVRENAQLLTVIAGSDRKDSTSAP
VQIADYTSKIGQDIKGMKIALPKEYLGEGIDPKIKETVLAAAKHFEKLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGFRAADAKSLEDIYVKTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFADYDLILGP
TAPTAAFELDTLNHDPVAMYLADLLTIPVNLAGLPAISIPAGFADGLPVGLQLIGPKYSEEVIYQAAAAFEATTDYHKQQ
PMIFGGDS
>Mature_487_residues
SFNHHTIEELHELLVAKDISAVELTKATLEDIKAREEAVGSFITIAEEAALKQAAALDAKGIDPDNVMSGIPLAVKDNIS
TKGILTTAASKMLYNYEPIFDATAVANAYDKDMIIIGKTNMDEFAMGGSTETSYFKKTKNAWDRSRVPGGSSGGSATAVA
SGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGAVSRYGLIAFGSSLDQIGPFAPTVRENAQLLTVIAGSDRKDSTSAPV
QIADYTSKIGQDIKGMKIALPKEYLGEGIDPKIKETVLAAAKHFEKLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGFRAADAKSLEDIYVKTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFADYDLILGPT
APTAAFELDTLNHDPVAMYLADLLTIPVNLAGLPAISIPAGFADGLPVGLQLIGPKYSEEVIYQAAAAFEATTDYHKQQP
MIFGGDS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=37.0741482965932, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=23.5173824130879, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=37.2505543237251, Blast_Score=271, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=22.4742268041237, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=28.1045751633987, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=32.8125, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=186, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=408, Percent_Identity=34.3137254901961, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=30, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320448, Length=197, Percent_Identity=31.4720812182741, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=40.3292181069959, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24652985, Length=483, Percent_Identity=25.6728778467909, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=483, Percent_Identity=25.6728778467909, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=483, Percent_Identity=25.6728778467909, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=483, Percent_Identity=25.6728778467909, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=23.5528942115768, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24644968, Length=509, Percent_Identity=24.3614931237721, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648435, Length=200, Percent_Identity=34, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648437, Length=200, Percent_Identity=34, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550774, Length=200, Percent_Identity=34, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356731, Length=500, Percent_Identity=23, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=130, Percent_Identity=40.7692307692308, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648439, Length=130, Percent_Identity=40.7692307692308, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52237; Mature: 52106

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNHHTIEELHELLVAKDISAVELTKATLEDIKAREEAVGSFITIAEEAALKQAAALDA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGIDPDNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFDATAVANAYDKDMIIIGKT
CCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDRSRVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGVVGLKPTYGAVSRYGLIAFGSSLDQIGPFAPTVRENAQLLTVIAGSDRKDSTSAP
CCCCCCEECCCCHHHHHHCCCEEECCCHHHCCCCCCCHHCCCEEEEEEECCCCCCCCCCC
VQIADYTSKIGQDIKGMKIALPKEYLGEGIDPKIKETVLAAAKHFEKLGAIIEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRAADAKSLEDIYVKTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHEEEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFADYDLILGPTAPTAAFELDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCCHHHHH
LADLLTIPVNLAGLPAISIPAGFADGLPVGLQLIGPKYSEEVIYQAAAAFEATTDYHKQQ
HHHHHHHCCCCCCCCEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
PMIFGGDS
CEEECCCC
>Mature Secondary Structure 
SFNHHTIEELHELLVAKDISAVELTKATLEDIKAREEAVGSFITIAEEAALKQAAALDA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGIDPDNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFDATAVANAYDKDMIIIGKT
CCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEECC
NMDEFAMGGSTETSYFKKTKNAWDRSRVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQP
CCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGVVGLKPTYGAVSRYGLIAFGSSLDQIGPFAPTVRENAQLLTVIAGSDRKDSTSAP
CCCCCCEECCCCHHHHHHCCCEEECCCHHHCCCCCCCHHCCCEEEEEEECCCCCCCCCCC
VQIADYTSKIGQDIKGMKIALPKEYLGEGIDPKIKETVLAAAKHFEKLGAIIEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGFRAADAKSLEDIYVKTRSQGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHEEEEE
TFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFADYDLILGPTAPTAAFELDTLNHDPVAMY
EEECCCCCHHHHHHCCCCEEHHHHHHHHHHHCCCCEEECCCCCCCEEEECCCCCCHHHHH
LADLLTIPVNLAGLPAISIPAGFADGLPVGLQLIGPKYSEEVIYQAAAAFEATTDYHKQQ
HHHHHHHCCCCCCCCEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
PMIFGGDS
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA