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The map label for this gene is gatB [H]

Identifier: 195977534

GI number: 195977534

Start: 419649

End: 421088

Strand: Direct

Name: gatB [H]

Synonym: Sez_0391

Alternate gene names: 195977534

Gene position: 419649-421088 (Clockwise)

Preceding gene: 195977533

Following gene: 195977535

Centisome position: 20.73

GC content: 46.94

Gene sequence:

>1440_bases
ATGAATTTTGAAACCATTATTGGACTTGAGGTCCATGTGGAGTTGAATACCAATTCTAAGATTTTCTCGCCTTCATCAGC
TCATTTTGGAGAGGATCCCAACGCCAGCACTAATGTGATTGACTGGTCTTTCCCAGGTGTCCTCCCAGTGATGAACAAGG
GAGTCATTGATGCGGGAATCAAAGCAGCACTTGCTCTTAACATGTCTATTCATCAGCACATGCATTTTGACCGTAAGAAT
TATTTCTACCCAGATAATCCCAAGGCCTACCAGATTTCACAGTTTGATGAGCCGATTGGCTACAATGGCTGGATTGATAT
TACTCTTGAGGACGGCTCAACCAAGAAAATCCGTATTGAGCGTGCTCACCTAGAGGAGGATGCCGGCAAAAATACGCATG
GTACAGATGGCTATTCCTATGTTGACCTCAATCGTCAGGGGGTGCCCTTGATTGAGATCGTCTCAGAGGCTGACATGCGC
TCTCCAGAGGAGGCTTATGCTTACCTGACTGCCCTAAAGGAAATCATTCAGTATACTGGTATTTCTGATGTTAAGATGGA
AGAGGGGTCCATGCGTGTGGACGCCAATATCTCCTTGCGACCATACGGACAAGAGGCCTTTGGTACAAAAACAGAATTGA
AAAACCTGAACTCATTTAGCAATGTGCGTAAGGGGCTTGAGTTTGAGGTGGAGCGTCAAGCCAAAATCCTACGCTCTGGC
GGTGTTATTCGTCAGGAGACACGTCGTTATGACGAGGCAAGTAAGGGAACGATTCTCATGCGTGTCAAGGAGGGAGCAGC
AGACTACCGCTATTTCCCAGAGCCAGATTTGCCATTGTTTGAGATTGATGACGCATGGATTGAGGATATGCGTGCAGAGC
TCCCAAGGTTCCCCGCAGAGCGTCGGGTAAGCTATATCAAGGATCTTGGTTTGTCCGCCTACGATGCAGGTCAGCTGACA
GCAACAAAGGCTTTATCTGATTTCTTTGAGCAGGCTGTGGCACTTGGTGGTGACGCAAAGCAAGTGTCTAATTGGCTCCA
GGGTGAGGTGGCTCAGTTCTTAAATGCTGAGGGCAAGACGATTGATCAGCTTGCCTTGACACCAGATAGCCTTGTGGAAA
TGATTGCGATCATCGCTGATGGCACGATTTCATCAAAAATAGCTAAGAAGGTCTTTGTTCATTTGGCAAAGCATGGCGGC
TCAGCGCGTGCTTATGTGGAAAAAGCAGGTCTGGTTCAAATTTCAGACCCAGCTGTCTTGATTCCAATCATTCATCAGGT
GTTTGCTGACAATGAGGCTGCTGTGGCAGATTTCAAATCAGGCAAGCGTAATGCTGATAAGGCCTTTACTGGTTTTCTGA
TGAAGGCCACAAAGGGGCAAGCCAACCCACAGATAGCTCAGCAATTGTTAGCCCAAGAATTGCAAAAATTGCTGGATTAA

Upstream 100 bases:

>100_bases
CAAAATATAGTGAAGAGGTCATCTATCAGGCAGCAGCAGCCTTTGAAGCGACTACTGACTACCACAAGCAGCAGCCTATG
ATTTTTGGAGGTGACAGCTA

Downstream 100 bases:

>100_bases
GGAGTCGTTATGACTGGCATTGAGTCTGAAACAGAGTTTTCAGGCTCTTTATGATGGTAGGCTCTTTAGGACAATTCTGT
GATAATACCTCGCTATCCGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNASTNVIDWSFPGVLPVMNKGVIDAGIKAALALNMSIHQHMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIDITLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEAFGTKTELKNLNSFSNVRKGLEFEVERQAKILRSG
GVIRQETRRYDEASKGTILMRVKEGAADYRYFPEPDLPLFEIDDAWIEDMRAELPRFPAERRVSYIKDLGLSAYDAGQLT
ATKALSDFFEQAVALGGDAKQVSNWLQGEVAQFLNAEGKTIDQLALTPDSLVEMIAIIADGTISSKIAKKVFVHLAKHGG
SARAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQIAQQLLAQELQKLLD

Sequences:

>Translated_479_residues
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNASTNVIDWSFPGVLPVMNKGVIDAGIKAALALNMSIHQHMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIDITLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEAFGTKTELKNLNSFSNVRKGLEFEVERQAKILRSG
GVIRQETRRYDEASKGTILMRVKEGAADYRYFPEPDLPLFEIDDAWIEDMRAELPRFPAERRVSYIKDLGLSAYDAGQLT
ATKALSDFFEQAVALGGDAKQVSNWLQGEVAQFLNAEGKTIDQLALTPDSLVEMIAIIADGTISSKIAKKVFVHLAKHGG
SARAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQIAQQLLAQELQKLLD
>Mature_479_residues
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNASTNVIDWSFPGVLPVMNKGVIDAGIKAALALNMSIHQHMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIDITLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEAFGTKTELKNLNSFSNVRKGLEFEVERQAKILRSG
GVIRQETRRYDEASKGTILMRVKEGAADYRYFPEPDLPLFEIDDAWIEDMRAELPRFPAERRVSYIKDLGLSAYDAGQLT
ATKALSDFFEQAVALGGDAKQVSNWLQGEVAQFLNAEGKTIDQLALTPDSLVEMIAIIADGTISSKIAKKVFVHLAKHGG
SARAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQIAQQLLAQELQKLLD

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=34.2105263157895, Blast_Score=279, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI115534094, Length=346, Percent_Identity=34.393063583815, Blast_Score=204, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=29.6442687747036, Blast_Score=199, Evalue=9e-52,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=31.9919517102616, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53129; Mature: 53129

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNASTNVIDWSFPGVLPVMNKGVIDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCHHHCC
KAALALNMSIHQHMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIDITLEDGSTKKIRIE
HHHHEEHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEIIQYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEAFGTKTELKNLNSFSNVRKGLEFEVERQAKILRSG
CCCEEECCCCEEEECCEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHC
GVIRQETRRYDEASKGTILMRVKEGAADYRYFPEPDLPLFEIDDAWIEDMRAELPRFPAE
CCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRVSYIKDLGLSAYDAGQLTATKALSDFFEQAVALGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
IDQLALTPDSLVEMIAIIADGTISSKIAKKVFVHLAKHGGSARAYVEKAGLVQISDPAVL
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHCCEEEECCCEEH
IPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQIAQQLLAQELQKLLD
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNASTNVIDWSFPGVLPVMNKGVIDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCHHHCC
KAALALNMSIHQHMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIDITLEDGSTKKIRIE
HHHHEEHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEIIQYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEAFGTKTELKNLNSFSNVRKGLEFEVERQAKILRSG
CCCEEECCCCEEEECCEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHC
GVIRQETRRYDEASKGTILMRVKEGAADYRYFPEPDLPLFEIDDAWIEDMRAELPRFPAE
CCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRVSYIKDLGLSAYDAGQLTATKALSDFFEQAVALGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
IDQLALTPDSLVEMIAIIADGTISSKIAKKVFVHLAKHGGSARAYVEKAGLVQISDPAVL
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHCCEEEECCCEEH
IPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQIAQQLLAQELQKLLD
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA