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The map label for this gene is amiA [H]

Identifier: 194335000

GI number: 194335000

Start: 2388888

End: 2390579

Strand: Reverse

Name: amiA [H]

Synonym: Paes_2211

Alternate gene names: 194335000

Gene position: 2390579-2388888 (Counterclockwise)

Preceding gene: 194335002

Following gene: 194334999

Centisome position: 95.13

GC content: 53.78

Gene sequence:

>1692_bases
ATGCTTTTCGTGTTGTTCGTCGGCATGTCAGCAGTTGCCGCGGCAGCGTCTCCTGAAGAACCCATAGTGACATCGGTTAC
CGTGCATGCGGTCAAGGGGTTTCAGCAGGAGTATTCACTCAAGGTGGCTGCCGTCATAAAGGATAAGGTGATGTCGGTTG
ATATGGGGGCATTGGCTCGGGCGTTGCGTCTCATTCACAAGCGCGAGGGCGATCATCTGGTGATCGAGAATACTCTTGAC
GGCAAGAGCTCTTTTTGCCATCTGTATGAGAAGAACAATTTCGTTCGGATCGACGCTGCGGATCCGTCGGGAGAGACCCG
TGTGATCCAGTTGCAATCGCCTCCCGTTCTCCGGGGAGAGACGTTGTATCTGCCTGTCGCAGACGCAGCAAGAATGTTTG
CTCTCTGGCTTGACAGGGAGGTTTCTTACGACAGAAAGCGTGGTCGTATCGATGCATTTTTATGGAGCTCCCGTCCCGGT
TCCGAAGTCGGGTCGATCGGGGTGGTCAAGCCGCAGGACCGTGATATCCGTGCAGGTAGTGCAGCCCGGCATACCGGGCT
GACCGAACTCACTGCCCTGAAGATCAATGAGCTGGTCAATGGCGTGGTGATCCGCATCAAGGCGACAGGAGCTGAGTCGG
TTGCCTCGTTTATCAAGCCGGACGCCAGTGGGATGGCCTATCTGACCTTCAAGAATGCCAAGGGGGACCCCTCGATGTTT
TTACGCTCCTTCAGCAAGGGACTTCTCAAGGAGATAAAGGCGATTCCTCTTGGCGGGGGAGCTATGCAGTTGAGCATGAG
CTTCAACAACACCTTGTTTAATCTCAAGTCCACACAGTATCACTGGGATGCGAGAACCAATACCTATATCATCTCTGTCT
TGACCGATGTCGATGTGCAGCAGGCCTACCGTCAGGAAAAGCTCAAGAGTATCCAGCAGGGGCTGCTTCATGATCAGCAG
AAATGGAAGTTCGATACCATTGTGCTCGATGCGGGTCATGGGGGGCGCGACCCTGGAGCTGTGGGGCCGGGAGGTACTCA
GGAAAAAGATGTGGTTCTCAATATTGTCAAAGAGCTTGGTCAGATTCTTCAGAAAGAGTGGCCGGATGTCAAGGTGATCT
ACACCAGAACGGATGACCGGCTTATTGCGCTCAAGCAGAGGGGGAAAATAGCCAATCAAAATGATGCCAAACTGTTTGTG
AGCGTGCACTGTAATGCGGCGAAAAACCGTAAGGCAGAAGGTGCCGAAGTCTACATTCTCGGTCCCCACAAGAATGATGC
GGCGCTGGAAGTTGCGATGCTTGAAAATGCGGCCATCAAGCAGGAAGAGGGGTATGAGGAGAAGTACAAGGGTGTTTCAG
AAGAACATATGATTCTCAGCAGCCTTGCGCAAAGCGCTTTTACCCTGCAGTCAACGACGGTTGCGCGTCACGTGCTCGAG
GGTATGGAGCAGAAAACCAGCATCAACGGCCGCGGCGTACGCCAGGCAGGCTTCATGGTATTGTGGACGCCCTCGATGCC
CAGCGTGCTCGTTGAAGCAGGCTATCTCTCTAATCCCAAGGAGGAGAAGCTTCTTCGCCAGGCTGGCGTTCAGCGCGATA
TTGCCAGGGGGATCTATAACGGACTTGTGCGTTATAGACAGCATTATGAACAGCAGCAGCTGGCGTCTATGGGTGAGCCG
GCAGGTCGTTGA

Upstream 100 bases:

>100_bases
TTGAACTTTGTGCAAACCATACCTGATTTACTATTCCATGATGAGGATTCTGTTCTCCAGCCGGCAATTCCGGTCAGTTT
TTTTTTGCTGTCGTCTCTGG

Downstream 100 bases:

>100_bases
CCCTGCGATTCGAGACTGAAATTCTTGTCACGACCTGCAGGAACAACGTTCTTTTTGTCGTGTTGTTGTTCTGCCCGGCA
ATCCAGAGGCAGAATCACGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MLFVLFVGMSAVAAAASPEEPIVTSVTVHAVKGFQQEYSLKVAAVIKDKVMSVDMGALARALRLIHKREGDHLVIENTLD
GKSSFCHLYEKNNFVRIDAADPSGETRVIQLQSPPVLRGETLYLPVADAARMFALWLDREVSYDRKRGRIDAFLWSSRPG
SEVGSIGVVKPQDRDIRAGSAARHTGLTELTALKINELVNGVVIRIKATGAESVASFIKPDASGMAYLTFKNAKGDPSMF
LRSFSKGLLKEIKAIPLGGGAMQLSMSFNNTLFNLKSTQYHWDARTNTYIISVLTDVDVQQAYRQEKLKSIQQGLLHDQQ
KWKFDTIVLDAGHGGRDPGAVGPGGTQEKDVVLNIVKELGQILQKEWPDVKVIYTRTDDRLIALKQRGKIANQNDAKLFV
SVHCNAAKNRKAEGAEVYILGPHKNDAALEVAMLENAAIKQEEGYEEKYKGVSEEHMILSSLAQSAFTLQSTTVARHVLE
GMEQKTSINGRGVRQAGFMVLWTPSMPSVLVEAGYLSNPKEEKLLRQAGVQRDIARGIYNGLVRYRQHYEQQQLASMGEP
AGR

Sequences:

>Translated_563_residues
MLFVLFVGMSAVAAAASPEEPIVTSVTVHAVKGFQQEYSLKVAAVIKDKVMSVDMGALARALRLIHKREGDHLVIENTLD
GKSSFCHLYEKNNFVRIDAADPSGETRVIQLQSPPVLRGETLYLPVADAARMFALWLDREVSYDRKRGRIDAFLWSSRPG
SEVGSIGVVKPQDRDIRAGSAARHTGLTELTALKINELVNGVVIRIKATGAESVASFIKPDASGMAYLTFKNAKGDPSMF
LRSFSKGLLKEIKAIPLGGGAMQLSMSFNNTLFNLKSTQYHWDARTNTYIISVLTDVDVQQAYRQEKLKSIQQGLLHDQQ
KWKFDTIVLDAGHGGRDPGAVGPGGTQEKDVVLNIVKELGQILQKEWPDVKVIYTRTDDRLIALKQRGKIANQNDAKLFV
SVHCNAAKNRKAEGAEVYILGPHKNDAALEVAMLENAAIKQEEGYEEKYKGVSEEHMILSSLAQSAFTLQSTTVARHVLE
GMEQKTSINGRGVRQAGFMVLWTPSMPSVLVEAGYLSNPKEEKLLRQAGVQRDIARGIYNGLVRYRQHYEQQQLASMGEP
AGR
>Mature_563_residues
MLFVLFVGMSAVAAAASPEEPIVTSVTVHAVKGFQQEYSLKVAAVIKDKVMSVDMGALARALRLIHKREGDHLVIENTLD
GKSSFCHLYEKNNFVRIDAADPSGETRVIQLQSPPVLRGETLYLPVADAARMFALWLDREVSYDRKRGRIDAFLWSSRPG
SEVGSIGVVKPQDRDIRAGSAARHTGLTELTALKINELVNGVVIRIKATGAESVASFIKPDASGMAYLTFKNAKGDPSMF
LRSFSKGLLKEIKAIPLGGGAMQLSMSFNNTLFNLKSTQYHWDARTNTYIISVLTDVDVQQAYRQEKLKSIQQGLLHDQQ
KWKFDTIVLDAGHGGRDPGAVGPGGTQEKDVVLNIVKELGQILQKEWPDVKVIYTRTDDRLIALKQRGKIANQNDAKLFV
SVHCNAAKNRKAEGAEVYILGPHKNDAALEVAMLENAAIKQEEGYEEKYKGVSEEHMILSSLAQSAFTLQSTTVARHVLE
GMEQKTSINGRGVRQAGFMVLWTPSMPSVLVEAGYLSNPKEEKLLRQAGVQRDIARGIYNGLVRYRQHYEQQQLASMGEP
AGR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=273, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790611, Length=232, Percent_Identity=34.4827586206897, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082163, Length=227, Percent_Identity=35.6828193832599, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 62120; Mature: 62120

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFVLFVGMSAVAAAASPEEPIVTSVTVHAVKGFQQEYSLKVAAVIKDKVMSVDMGALAR
CEEEEEECHHHHHHCCCCCCCCEEHEEHHHHHHHHHHHCEEEEEEHHHHHHCCCHHHHHH
ALRLIHKREGDHLVIENTLDGKSSFCHLYEKNNFVRIDAADPSGETRVIQLQSPPVLRGE
HHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCC
TLYLPVADAARMFALWLDREVSYDRKRGRIDAFLWSSRPGSEVGSIGVVKPQDRDIRAGS
EEEEECHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCC
AARHTGLTELTALKINELVNGVVIRIKATGAESVASFIKPDASGMAYLTFKNAKGDPSMF
CHHHCCCHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
LRSFSKGLLKEIKAIPLGGGAMQLSMSFNNTLFNLKSTQYHWDARTNTYIISVLTDVDVQ
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCEEEECCCCCEEEEEEEECCHHH
QAYRQEKLKSIQQGLLHDQQKWKFDTIVLDAGHGGRDPGAVGPGGTQEKDVVLNIVKELG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QILQKEWPDVKVIYTRTDDRLIALKQRGKIANQNDAKLFVSVHCNAAKNRKAEGAEVYIL
HHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEE
GPHKNDAALEVAMLENAAIKQEEGYEEKYKGVSEEHMILSSLAQSAFTLQSTTVARHVLE
CCCCCCCEEEEEEHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMEQKTSINGRGVRQAGFMVLWTPSMPSVLVEAGYLSNPKEEKLLRQAGVQRDIARGIYN
HHHHHCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
GLVRYRQHYEQQQLASMGEPAGR
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLFVLFVGMSAVAAAASPEEPIVTSVTVHAVKGFQQEYSLKVAAVIKDKVMSVDMGALAR
CEEEEEECHHHHHHCCCCCCCCEEHEEHHHHHHHHHHHCEEEEEEHHHHHHCCCHHHHHH
ALRLIHKREGDHLVIENTLDGKSSFCHLYEKNNFVRIDAADPSGETRVIQLQSPPVLRGE
HHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCEEEEEECCCCCCCCC
TLYLPVADAARMFALWLDREVSYDRKRGRIDAFLWSSRPGSEVGSIGVVKPQDRDIRAGS
EEEEECHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCC
AARHTGLTELTALKINELVNGVVIRIKATGAESVASFIKPDASGMAYLTFKNAKGDPSMF
CHHHCCCHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
LRSFSKGLLKEIKAIPLGGGAMQLSMSFNNTLFNLKSTQYHWDARTNTYIISVLTDVDVQ
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCEEEECCCCCEEEEEEEECCHHH
QAYRQEKLKSIQQGLLHDQQKWKFDTIVLDAGHGGRDPGAVGPGGTQEKDVVLNIVKELG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QILQKEWPDVKVIYTRTDDRLIALKQRGKIANQNDAKLFVSVHCNAAKNRKAEGAEVYIL
HHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEE
GPHKNDAALEVAMLENAAIKQEEGYEEKYKGVSEEHMILSSLAQSAFTLQSTTVARHVLE
CCCCCCCEEEEEEHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMEQKTSINGRGVRQAGFMVLWTPSMPSVLVEAGYLSNPKEEKLLRQAGVQRDIARGIYN
HHHHHCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
GLVRYRQHYEQQQLASMGEPAGR
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]