Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 194334473

GI number: 194334473

Start: 1815960

End: 1816838

Strand: Reverse

Name: purU [H]

Synonym: Paes_1668

Alternate gene names: 194334473

Gene position: 1816838-1815960 (Counterclockwise)

Preceding gene: 194334478

Following gene: 194334472

Centisome position: 72.3

GC content: 51.31

Gene sequence:

>879_bases
ATGAACCGCATCGATAACCTTCAGCCGTCTCTGTCGGCGATACTTCTCCTTGCGTGTCCTGATCGGAGAGGTCTTGTTTC
TCGACTTGCTCATTTTATTTTTGAGCACGGAGGTAACATTCTTGACCTTGACGAGCATGTTGACGCCGGGTCGGGCATGT
TTTTTATCAGGGTATCATGGAGTATGGACGGGTTTTCCATTCCTGCATCGGATCTGGAGGAGGTATTTCGTCCTCTTGGA
CGGGAGTTTCAGGCAAGGTGGAGTATTCATACGGCCGAGACCAGAGAGCGCGTTGCGCTTTTCGTATCGAAGTATGACCA
TTGTCTTCAGGAGATTTTGTGGCGTCACAGAACCGGCGAGTTTCAGATCGATATTCCCCTTATTATTTCCAACCACCCTG
ATCTTGGGCCTCTGGCGCGTCATTACGGAATCGCATTTCATGTCTACCCTATAACGAGTGAGAATAAATTGGATCAGGAG
CAGCGTGAACTTGAGCTGCTCAGGGCGCATCGGATTGATACCGTGGTGCTTGCCCGTTATATGCAGGTGTTGTCGGACCG
CTTTGTCGACGCTATGCCTGAGCGGGTGATCAATATCCACCACTCTTTTCTGCCGGCGTTTTCAGGCGGAAATCCCTATC
GTCAGGCGTTTGAGCGTGGGGTTAAAATTATTGGCGCTACCAGTCACTATGTGACTGCAGAGCTTGACGAAGGGCCTATT
ATCGAGCAGGATATTGTCCGTATTTCCCATAAGGACACCCTGCCGGACCTTGTCAGGAAAGGGCGCGATCTGGAGCGTCT
TGTGCTGGCAAGGGCCCTTTCGCGGCATGTGGAGCACCGGGTGCTGGTGAACGGGCGTAAAACGATTGTTTTTGAATGA

Upstream 100 bases:

>100_bases
AGAACTTTTAGTGACGGTCAATGATTAAATCATTCTATAAAAACAAGGTGCCTCACTGGAGACATCTCCCTGGAAGATCA
TTCATTTTTTTGTTTCATCT

Downstream 100 bases:

>100_bases
CCGCTCTTTTTTTCTGTTAACCTCTTTTTACAAAGGAGATAGTTTGATGTTAAAACGGTTTTTGTTTTTACTTCCTGTTC
TGCTGATGGGCTGTATCAAG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNRIDNLQPSLSAILLLACPDRRGLVSRLAHFIFEHGGNILDLDEHVDAGSGMFFIRVSWSMDGFSIPASDLEEVFRPLG
REFQARWSIHTAETRERVALFVSKYDHCLQEILWRHRTGEFQIDIPLIISNHPDLGPLARHYGIAFHVYPITSENKLDQE
QRELELLRAHRIDTVVLARYMQVLSDRFVDAMPERVINIHHSFLPAFSGGNPYRQAFERGVKIIGATSHYVTAELDEGPI
IEQDIVRISHKDTLPDLVRKGRDLERLVLARALSRHVEHRVLVNGRKTIVFE

Sequences:

>Translated_292_residues
MNRIDNLQPSLSAILLLACPDRRGLVSRLAHFIFEHGGNILDLDEHVDAGSGMFFIRVSWSMDGFSIPASDLEEVFRPLG
REFQARWSIHTAETRERVALFVSKYDHCLQEILWRHRTGEFQIDIPLIISNHPDLGPLARHYGIAFHVYPITSENKLDQE
QRELELLRAHRIDTVVLARYMQVLSDRFVDAMPERVINIHHSFLPAFSGGNPYRQAFERGVKIIGATSHYVTAELDEGPI
IEQDIVRISHKDTLPDLVRKGRDLERLVLARALSRHVEHRVLVNGRKTIVFE
>Mature_292_residues
MNRIDNLQPSLSAILLLACPDRRGLVSRLAHFIFEHGGNILDLDEHVDAGSGMFFIRVSWSMDGFSIPASDLEEVFRPLG
REFQARWSIHTAETRERVALFVSKYDHCLQEILWRHRTGEFQIDIPLIISNHPDLGPLARHYGIAFHVYPITSENKLDQE
QRELELLRAHRIDTVVLARYMQVLSDRFVDAMPERVINIHHSFLPAFSGGNPYRQAFERGVKIIGATSHYVTAELDEGPI
IEQDIVRISHKDTLPDLVRKGRDLERLVLARALSRHVEHRVLVNGRKTIVFE

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=31.9148936170213, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=31.9148936170213, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=31.9148936170213, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1787483, Length=283, Percent_Identity=39.9293286219081, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1788846, Length=188, Percent_Identity=30.8510638297872, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582400, Length=213, Percent_Identity=32.3943661971831, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33562; Mature: 33562

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDNLQPSLSAILLLACPDRRGLVSRLAHFIFEHGGNILDLDEHVDAGSGMFFIRVSW
CCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCEEECHHCCCCCCCEEEEEEEE
SMDGFSIPASDLEEVFRPLGREFQARWSIHTAETRERVALFVSKYDHCLQEILWRHRTGE
CCCCCCCCHHHHHHHHHHHCHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FQIDIPLIISNHPDLGPLARHYGIAFHVYPITSENKLDQEQRELELLRAHRIDTVVLARY
EEEEEEEEECCCCCCCHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MQVLSDRFVDAMPERVINIHHSFLPAFSGGNPYRQAFERGVKIIGATSHYVTAELDEGPI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCEEEEECCCCCC
IEQDIVRISHKDTLPDLVRKGRDLERLVLARALSRHVEHRVLVNGRKTIVFE
CHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEC
>Mature Secondary Structure
MNRIDNLQPSLSAILLLACPDRRGLVSRLAHFIFEHGGNILDLDEHVDAGSGMFFIRVSW
CCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCEEECHHCCCCCCCEEEEEEEE
SMDGFSIPASDLEEVFRPLGREFQARWSIHTAETRERVALFVSKYDHCLQEILWRHRTGE
CCCCCCCCHHHHHHHHHHHCHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FQIDIPLIISNHPDLGPLARHYGIAFHVYPITSENKLDQEQRELELLRAHRIDTVVLARY
EEEEEEEEECCCCCCCHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MQVLSDRFVDAMPERVINIHHSFLPAFSGGNPYRQAFERGVKIIGATSHYVTAELDEGPI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCEEEEECCCCCC
IEQDIVRISHKDTLPDLVRKGRDLERLVLARALSRHVEHRVLVNGRKTIVFE
CHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]