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The map label for this gene is recJ [H]

Identifier: 194334478

GI number: 194334478

Start: 1821114

End: 1822826

Strand: Reverse

Name: recJ [H]

Synonym: Paes_1673

Alternate gene names: 194334478

Gene position: 1822826-1821114 (Counterclockwise)

Preceding gene: 194334482

Following gene: 194334473

Centisome position: 72.54

GC content: 51.72

Gene sequence:

>1713_bases
ATGAAACGATTCCGCTGGACGATCCGGGATAGCGATGATGAGGCCTGTGAGCGGCTTTCCCGGGAGATTAATGTGAGTCT
GCCGATTGCCCGTGCACTCTGCAATCGAGGAATCTGTGATTTCGACCAGGCAAAAGCTTTTTTCCGGCCTGATCCTGCCG
TGATTCCTTCACCGTTTCTTTTCAGGGATATGCAGTGCGCTGTTGAGCGCGTGATGAAGGCGCTGGAACAGGGTGAGCAT
ATTCTGCTCTATGGTGATTATGATGTGGACGGTACGACGGGGACGGCTCTTCTGATGCTTTTTCTTCGTTCACAGGGTGC
AGAGGTTTCCTATTATATCAATGACCGGTTCACTGAGGGCTACGGATTATCGGATGCAGGGGTTGGGTGCGCGGTTGAAC
GAGGTGCCGGACTGGTTATCACGGTGGATTGCGGTATCAGGGCGGTGAAGGAGGTCGAGGCGTTCAATGTTCAAGGGATC
GATGTTATTGTCTGCGATCATCATGAACCCGGAACGCTTCCTGCCGCCTCTGCGATTCTGAACCCGAAAGTACCTGGATG
CAGTTATCCTTTCAGGGAGTTATGCGGTTGCGGTGTTGCGTTTAAGCTGGTTCAGGCATTGATTGAACAGATGGGAAGGG
ATGCTGCTGAGTGGGAGCAGTATCTCGATCTTGTTGCTGTAGCTACTCTTGCCGATATGGTCGCGCTGAAGGAGGAGAAC
CGTATTTATATGCATCAGGGACTCCAGCGCCTCAAAGCTGCACCGCGTATGAGTATACAGGAACTTATCTCCTGTATGGG
CGCCAGGCTGGAATCGCTGTCAGCCGGTGTGATAGCCTTCGGTATTGCTCCAAGAATCAATGCTGCCGGCAGGATGCAGA
ATGCGGCGACTGCCGTTGAATGGCTGATGTCCGACTCCCGTGATGAGTGCAGGAAGCATGCAGAGGAACTTGACCGGCTG
AATGGGGAGCGGCGTGAGATCGATGCCGGTATTCTGAAACTCGCAGAGGGTATGGTGAATGGTTACTTTGCCAGTTACTG
TTCATCGCTTGTCCTCTATCACGAGGATTGGCATCTTGGCGTGCTTGGTATTGTCGCATCGCGTCTTCAGGAGAAATATT
ACCTGCCGACGGTTGTGTTGGGGGGCAGCGATGGTCTTATCAAGGGGTCTGTCAGAAGTGTTGGCGGTTTGAATGTGATG
GAAGTTCTTCAGGAGTGCAGCGGATTGCTTGAGAAATTTGGCGGTCATGAGGCTGCTGCGGGGGTGACGCTCAAACCTGA
GAACCTGCAGCTTTTCAGAAAACGCTTCGATGCTCTCTCGGCAGAACGATTGAGTTATGAACAGCGCCAGAAGGAGGTGA
TCATCGATACGCAATTACAGCTTGACGAGGTGAGCCCTAAATTTATCAATGTTCTTTCACAGTTCGCTCCTTTTGGATTT
GGTAATCCCGAACCTGTGTTTCTCAGCCGCGGGCTCAGAATTTCGGGCTACCCCCGTCTGCTGAAAGGCCGCCATGTGAA
GTTTTCGGTTAAAACCGGAAGCGGCAGGATTTTCGATGTTATCGGTTTCAGTCGTGAGGATATTTATCGTGCTCTTGAAC
GTGGATCGAAGCAGGTTTTTTCGATGGTCTACACTCTTGAGGAGAACGAATGGAACGGCAGGAAGACGATACAGATGAAG
CTGAAGGATATGGCCGTCGAGAAGTCACAATGA

Upstream 100 bases:

>100_bases
AGGCTATTTCAAGCGCTGAACCCTTCCGGATCATTTGCCTTCTGCTGATGAAATAGTTATATAAACGAACTTTTATACTA
ACCGTCAAGAATGTTATCAG

Downstream 100 bases:

>100_bases
CCCAATCGATGCAGGACGTGAGGGATGAAGCTCACGTCACTCCTTCTTCGTGACCTGTGCGTTTTTTTCTCCTCTGTCCG
AGCCAGTGGAGCGATTCTGG

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKRFRWTIRDSDDEACERLSREINVSLPIARALCNRGICDFDQAKAFFRPDPAVIPSPFLFRDMQCAVERVMKALEQGEH
ILLYGDYDVDGTTGTALLMLFLRSQGAEVSYYINDRFTEGYGLSDAGVGCAVERGAGLVITVDCGIRAVKEVEAFNVQGI
DVIVCDHHEPGTLPAASAILNPKVPGCSYPFRELCGCGVAFKLVQALIEQMGRDAAEWEQYLDLVAVATLADMVALKEEN
RIYMHQGLQRLKAAPRMSIQELISCMGARLESLSAGVIAFGIAPRINAAGRMQNAATAVEWLMSDSRDECRKHAEELDRL
NGERREIDAGILKLAEGMVNGYFASYCSSLVLYHEDWHLGVLGIVASRLQEKYYLPTVVLGGSDGLIKGSVRSVGGLNVM
EVLQECSGLLEKFGGHEAAAGVTLKPENLQLFRKRFDALSAERLSYEQRQKEVIIDTQLQLDEVSPKFINVLSQFAPFGF
GNPEPVFLSRGLRISGYPRLLKGRHVKFSVKTGSGRIFDVIGFSREDIYRALERGSKQVFSMVYTLEENEWNGRKTIQMK
LKDMAVEKSQ

Sequences:

>Translated_570_residues
MKRFRWTIRDSDDEACERLSREINVSLPIARALCNRGICDFDQAKAFFRPDPAVIPSPFLFRDMQCAVERVMKALEQGEH
ILLYGDYDVDGTTGTALLMLFLRSQGAEVSYYINDRFTEGYGLSDAGVGCAVERGAGLVITVDCGIRAVKEVEAFNVQGI
DVIVCDHHEPGTLPAASAILNPKVPGCSYPFRELCGCGVAFKLVQALIEQMGRDAAEWEQYLDLVAVATLADMVALKEEN
RIYMHQGLQRLKAAPRMSIQELISCMGARLESLSAGVIAFGIAPRINAAGRMQNAATAVEWLMSDSRDECRKHAEELDRL
NGERREIDAGILKLAEGMVNGYFASYCSSLVLYHEDWHLGVLGIVASRLQEKYYLPTVVLGGSDGLIKGSVRSVGGLNVM
EVLQECSGLLEKFGGHEAAAGVTLKPENLQLFRKRFDALSAERLSYEQRQKEVIIDTQLQLDEVSPKFINVLSQFAPFGF
GNPEPVFLSRGLRISGYPRLLKGRHVKFSVKTGSGRIFDVIGFSREDIYRALERGSKQVFSMVYTLEENEWNGRKTIQMK
LKDMAVEKSQ
>Mature_570_residues
MKRFRWTIRDSDDEACERLSREINVSLPIARALCNRGICDFDQAKAFFRPDPAVIPSPFLFRDMQCAVERVMKALEQGEH
ILLYGDYDVDGTTGTALLMLFLRSQGAEVSYYINDRFTEGYGLSDAGVGCAVERGAGLVITVDCGIRAVKEVEAFNVQGI
DVIVCDHHEPGTLPAASAILNPKVPGCSYPFRELCGCGVAFKLVQALIEQMGRDAAEWEQYLDLVAVATLADMVALKEEN
RIYMHQGLQRLKAAPRMSIQELISCMGARLESLSAGVIAFGIAPRINAAGRMQNAATAVEWLMSDSRDECRKHAEELDRL
NGERREIDAGILKLAEGMVNGYFASYCSSLVLYHEDWHLGVLGIVASRLQEKYYLPTVVLGGSDGLIKGSVRSVGGLNVM
EVLQECSGLLEKFGGHEAAAGVTLKPENLQLFRKRFDALSAERLSYEQRQKEVIIDTQLQLDEVSPKFINVLSQFAPFGF
GNPEPVFLSRGLRISGYPRLLKGRHVKFSVKTGSGRIFDVIGFSREDIYRALERGSKQVFSMVYTLEENEWNGRKTIQMK
LKDMAVEKSQ

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=529, Percent_Identity=37.0510396975425, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 63343; Mature: 63343

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFRWTIRDSDDEACERLSREINVSLPIARALCNRGICDFDQAKAFFRPDPAVIPSPFL
CCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHCCHHCCCCCCCCCCHHH
FRDMQCAVERVMKALEQGEHILLYGDYDVDGTTGTALLMLFLRSQGAEVSYYINDRFTEG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
YGLSDAGVGCAVERGAGLVITVDCGIRAVKEVEAFNVQGIDVIVCDHHEPGTLPAASAIL
CCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHC
NPKVPGCSYPFRELCGCGVAFKLVQALIEQMGRDAAEWEQYLDLVAVATLADMVALKEEN
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RIYMHQGLQRLKAAPRMSIQELISCMGARLESLSAGVIAFGIAPRINAAGRMQNAATAVE
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHH
WLMSDSRDECRKHAEELDRLNGERREIDAGILKLAEGMVNGYFASYCSSLVLYHEDWHLG
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHH
VLGIVASRLQEKYYLPTVVLGGSDGLIKGSVRSVGGLNVMEVLQECSGLLEKFGGHEAAA
HHHHHHHHHHHHHCCCEEEECCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
GVTLKPENLQLFRKRFDALSAERLSYEQRQKEVIIDTQLQLDEVSPKFINVLSQFAPFGF
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEHHHCCHHHHHHHHHHCCCCC
GNPEPVFLSRGLRISGYPRLLKGRHVKFSVKTGSGRIFDVIGFSREDIYRALERGSKQVF
CCCCCCCEECCCEECCCHHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHH
SMVYTLEENEWNGRKTIQMKLKDMAVEKSQ
HHHHHEECCCCCCCEEEEEEHHHHHCCCCC
>Mature Secondary Structure
MKRFRWTIRDSDDEACERLSREINVSLPIARALCNRGICDFDQAKAFFRPDPAVIPSPFL
CCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHCCHHCCCCCCCCCCHHH
FRDMQCAVERVMKALEQGEHILLYGDYDVDGTTGTALLMLFLRSQGAEVSYYINDRFTEG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
YGLSDAGVGCAVERGAGLVITVDCGIRAVKEVEAFNVQGIDVIVCDHHEPGTLPAASAIL
CCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHC
NPKVPGCSYPFRELCGCGVAFKLVQALIEQMGRDAAEWEQYLDLVAVATLADMVALKEEN
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RIYMHQGLQRLKAAPRMSIQELISCMGARLESLSAGVIAFGIAPRINAAGRMQNAATAVE
EEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHH
WLMSDSRDECRKHAEELDRLNGERREIDAGILKLAEGMVNGYFASYCSSLVLYHEDWHLG
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHH
VLGIVASRLQEKYYLPTVVLGGSDGLIKGSVRSVGGLNVMEVLQECSGLLEKFGGHEAAA
HHHHHHHHHHHHHCCCEEEECCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
GVTLKPENLQLFRKRFDALSAERLSYEQRQKEVIIDTQLQLDEVSPKFINVLSQFAPFGF
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEHHHCCHHHHHHHHHHCCCCC
GNPEPVFLSRGLRISGYPRLLKGRHVKFSVKTGSGRIFDVIGFSREDIYRALERGSKQVF
CCCCCCCEECCCEECCCHHHHCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHH
SMVYTLEENEWNGRKTIQMKLKDMAVEKSQ
HHHHHEECCCCCCCEEEEEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]