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The map label for this gene is mltD [H]

Identifier: 194334163

GI number: 194334163

Start: 1473636

End: 1475621

Strand: Reverse

Name: mltD [H]

Synonym: Paes_1354

Alternate gene names: 194334163

Gene position: 1475621-1473636 (Counterclockwise)

Preceding gene: 194334164

Following gene: 194334162

Centisome position: 58.72

GC content: 49.09

Gene sequence:

>1986_bases
ATGTCTCAACGAACTTCTTTTTCAGCACTTACACTCTTATTCTGCCTGCTGATTGCGACAATGCTGCAGCCAGGAAGGGC
GTATGCAGCCCGTTCTCAGGATGATCAGGCTGTTGCGGCTGTACTGGACAGCCTGGTTGCTGCGACTTATTTTGATGATA
GTCATTTCCGGCAATGGAAAGATGATGTGCCGAATGGCTTTTCTCCGACCTTTATTCCCCAGTTCGATGATGAGACATAT
GCAGAAAGGATAGCTGAACTCAACCGCAGAACGCCCATAAGACTGACCTACAACGAGCATGTAAAAAATTTTATAAAATT
GTATGCCGTTGACAAACGGGTGATGACAGCAAAGATTCTTGGTTTGACCAAAACCTATTTTCCCCTTTTTGAGCAAAAGC
TGCGCGAATACAATATGCCTACCGAGCTGAAATATCTTGCCATTGTCGAATCGGCACTCAACCCTGTTGCGGTATCGAGT
GCCAATGCAAAAGGGCTGTGGCAGTTTATTTACGGTACAGGCAAAATGTATGGTTTGAAGTCCACCTCATTTGTCGAGGA
TCGATTTGATCCCTATAAGGCGACTGTTGCCGCGTGTGAACACATGACGGATCTGTATGATATCTATGGCGACTGGTTTC
TTGTGCTCGCGGCATACAATGCCGGTGCCGGTAACGTCAATAAGGCTATCAGGCGATCAGGAGGACGGAGGGATTACTGG
GGGATATGGTCGAAGCTTCCTTCGGAGACACGGAGTTATGTTCCGGCATTCATTGCGGTGACCTATGTGATGAATTATTA
CAAGGAACACAATATCATTCCTGAAGAACCGGGGTATCTCTATGAGAATATCGCAACGGTCAATGTCTCTGATGTTCTGG
CTTTTGACCAGATCCACGAAGTGCTCGGTATTCCTCTCCAGGACCTTGAGTTTCTCAACCCGCAGTACAAGCGGGGGGTT
ATTCCCGTTATTGGCCCGGCACCGCTTTCGCTGCGTCTTCCAGACCATTATGTGGATGATTTTAATCGTAAGGAAAAAGA
GCTTTACGTGCATAAATCACAATCAGGGATTGACCGCGCTCAGCTTTTGGCTTCTGTCAAAGAGGGGGGGAAAGAGAGAA
AAATTCATCGGGTGAGACGTGGCGAAACGCTTGCATCAATTGCCAGAATGCATCGTTGTTATGTCAGTCAGTTGATCAAA
TGGAACAATCTTCGCAACTCGCGTATCTATCCCGGTCAGAAACTCTCTGTATTCGGCGCTCCTGAAACCATCCGCAGGTC
TTCGAAGCCGTCTTCGGCACCGTCGATAGTCTACCATAAAGTTCGAAAGGGAGAGTCGCTTATCAGGATTGCCAAGCGCT
ACAAGGTAAGCGTCAGCAATCTCGCCTCATGGAACAATCTTGGTCGGCGCCGCACCATCTATGCTGGTCAGAAGCTTAAA
ATATATGGCCGTGGTTCAGGTTCGACGTCGTCCTCTTCCAGCGTGTCGCAAACCCATATTGTCCGTCGTGGAGAGACCCT
TGGAGCGATTGCCAAGCGCTACAAGGTCAGCGTCAGCAATATCGCTTCATGGAACAGTCTCGGTCGGCGCCGTACTATCT
ATCCGGGTCAGAAGCTCAAAATATATGGCCGTGGTTCAGGTTCGACGTCGTCTTCTTCCAGCGTGTCGCAAACCCATATT
GTCCGTCGTGGAGAAACCCTTGGAGCGATTGCCAAGCGCTACAAGGTGAGCGTCAGCAATATCGCTTCATGGAACAGTCT
CGGTCGGCGCCGCACTATCTATCCGGGTCAGAAACTCAAAATAAACGGCAGGACATCAGGTTCGACGTCAGGAGCAGCCA
GGACGTATATTGTTAAACGGGGCGATACGCTGGGCGCGATCGCGAGTCGCTACAAGGTGAGCGTCAGCAAGATTGTCTCC
TGGAATAATCTGGGAAAGCGTCGCCTTATCTATCCAGGTCAGAAACTGAAAGTTTCTTCACATTAA

Upstream 100 bases:

>100_bases
TCACTAACTCTGTTTATGTTCTTGAGACCGGCTTCAGGTCGATAGCCAGAACGGTTCTGAGCTTCAATTTCTCAACATTT
TTTCAGTCACACGGCAACCC

Downstream 100 bases:

>100_bases
GCTACTCTTTTTTTTGTTCCAGGATGGAGTGAAGCGGCTTTTTGAACGTGTCGTTTGTTGGTTTTGATGAATGCAAACGC
GGGGTTGTGTTTTGTCTGAT

Product: lytic transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MSQRTSFSALTLLFCLLIATMLQPGRAYAARSQDDQAVAAVLDSLVAATYFDDSHFRQWKDDVPNGFSPTFIPQFDDETY
AERIAELNRRTPIRLTYNEHVKNFIKLYAVDKRVMTAKILGLTKTYFPLFEQKLREYNMPTELKYLAIVESALNPVAVSS
ANAKGLWQFIYGTGKMYGLKSTSFVEDRFDPYKATVAACEHMTDLYDIYGDWFLVLAAYNAGAGNVNKAIRRSGGRRDYW
GIWSKLPSETRSYVPAFIAVTYVMNYYKEHNIIPEEPGYLYENIATVNVSDVLAFDQIHEVLGIPLQDLEFLNPQYKRGV
IPVIGPAPLSLRLPDHYVDDFNRKEKELYVHKSQSGIDRAQLLASVKEGGKERKIHRVRRGETLASIARMHRCYVSQLIK
WNNLRNSRIYPGQKLSVFGAPETIRRSSKPSSAPSIVYHKVRKGESLIRIAKRYKVSVSNLASWNNLGRRRTIYAGQKLK
IYGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLKIYGRGSGSTSSSSSVSQTHI
VRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLKINGRTSGSTSGAARTYIVKRGDTLGAIASRYKVSVSKIVS
WNNLGKRRLIYPGQKLKVSSH

Sequences:

>Translated_661_residues
MSQRTSFSALTLLFCLLIATMLQPGRAYAARSQDDQAVAAVLDSLVAATYFDDSHFRQWKDDVPNGFSPTFIPQFDDETY
AERIAELNRRTPIRLTYNEHVKNFIKLYAVDKRVMTAKILGLTKTYFPLFEQKLREYNMPTELKYLAIVESALNPVAVSS
ANAKGLWQFIYGTGKMYGLKSTSFVEDRFDPYKATVAACEHMTDLYDIYGDWFLVLAAYNAGAGNVNKAIRRSGGRRDYW
GIWSKLPSETRSYVPAFIAVTYVMNYYKEHNIIPEEPGYLYENIATVNVSDVLAFDQIHEVLGIPLQDLEFLNPQYKRGV
IPVIGPAPLSLRLPDHYVDDFNRKEKELYVHKSQSGIDRAQLLASVKEGGKERKIHRVRRGETLASIARMHRCYVSQLIK
WNNLRNSRIYPGQKLSVFGAPETIRRSSKPSSAPSIVYHKVRKGESLIRIAKRYKVSVSNLASWNNLGRRRTIYAGQKLK
IYGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLKIYGRGSGSTSSSSSVSQTHI
VRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLKINGRTSGSTSGAARTYIVKRGDTLGAIASRYKVSVSKIVS
WNNLGKRRLIYPGQKLKVSSH
>Mature_660_residues
SQRTSFSALTLLFCLLIATMLQPGRAYAARSQDDQAVAAVLDSLVAATYFDDSHFRQWKDDVPNGFSPTFIPQFDDETYA
ERIAELNRRTPIRLTYNEHVKNFIKLYAVDKRVMTAKILGLTKTYFPLFEQKLREYNMPTELKYLAIVESALNPVAVSSA
NAKGLWQFIYGTGKMYGLKSTSFVEDRFDPYKATVAACEHMTDLYDIYGDWFLVLAAYNAGAGNVNKAIRRSGGRRDYWG
IWSKLPSETRSYVPAFIAVTYVMNYYKEHNIIPEEPGYLYENIATVNVSDVLAFDQIHEVLGIPLQDLEFLNPQYKRGVI
PVIGPAPLSLRLPDHYVDDFNRKEKELYVHKSQSGIDRAQLLASVKEGGKERKIHRVRRGETLASIARMHRCYVSQLIKW
NNLRNSRIYPGQKLSVFGAPETIRRSSKPSSAPSIVYHKVRKGESLIRIAKRYKVSVSNLASWNNLGRRRTIYAGQKLKI
YGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLKIYGRGSGSTSSSSSVSQTHIV
RRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLKINGRTSGSTSGAARTYIVKRGDTLGAIASRYKVSVSKIVSW
NNLGKRRLIYPGQKLKVSSH

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=453, Percent_Identity=31.1258278145695, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74406; Mature: 74275

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRTSFSALTLLFCLLIATMLQPGRAYAARSQDDQAVAAVLDSLVAATYFDDSHFRQWK
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
DDVPNGFSPTFIPQFDDETYAERIAELNRRTPIRLTYNEHVKNFIKLYAVDKRVMTAKIL
HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
GLTKTYFPLFEQKLREYNMPTELKYLAIVESALNPVAVSSANAKGLWQFIYGTGKMYGLK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHEECCCCEEECC
STSFVEDRFDPYKATVAACEHMTDLYDIYGDWFLVLAAYNAGAGNVNKAIRRSGGRRDYW
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCCCHHHHHHHHCCCCCCHH
GIWSKLPSETRSYVPAFIAVTYVMNYYKEHNIIPEEPGYLYENIATVNVSDVLAFDQIHE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCEEEECHHHHHHHHHHHH
VLGIPLQDLEFLNPQYKRGVIPVIGPAPLSLRLPDHYVDDFNRKEKELYVHKSQSGIDRA
HHCCCHHHHHHCCCHHHCCCEEEECCCCCEEECCHHHHHHCCCCCHHEEEECCCCCCHHH
QLLASVKEGGKERKIHRVRRGETLASIARMHRCYVSQLIKWNNLRNSRIYPGQKLSVFGA
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PETIRRSSKPSSAPSIVYHKVRKGESLIRIAKRYKVSVSNLASWNNLGRRRTIYAGQKLK
CHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEE
IYGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLK
EEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IYGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLK
EEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
INGRTSGSTSGAARTYIVKRGDTLGAIASRYKVSVSKIVSWNNLGKRRLIYPGQKLKVSS
ECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECC
H
C
>Mature Secondary Structure 
SQRTSFSALTLLFCLLIATMLQPGRAYAARSQDDQAVAAVLDSLVAATYFDDSHFRQWK
CCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
DDVPNGFSPTFIPQFDDETYAERIAELNRRTPIRLTYNEHVKNFIKLYAVDKRVMTAKIL
HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
GLTKTYFPLFEQKLREYNMPTELKYLAIVESALNPVAVSSANAKGLWQFIYGTGKMYGLK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHEECCCCEEECC
STSFVEDRFDPYKATVAACEHMTDLYDIYGDWFLVLAAYNAGAGNVNKAIRRSGGRRDYW
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCCCHHHHHHHHCCCCCCHH
GIWSKLPSETRSYVPAFIAVTYVMNYYKEHNIIPEEPGYLYENIATVNVSDVLAFDQIHE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCEEEECHHHHHHHHHHHH
VLGIPLQDLEFLNPQYKRGVIPVIGPAPLSLRLPDHYVDDFNRKEKELYVHKSQSGIDRA
HHCCCHHHHHHCCCHHHCCCEEEECCCCCEEECCHHHHHHCCCCCHHEEEECCCCCCHHH
QLLASVKEGGKERKIHRVRRGETLASIARMHRCYVSQLIKWNNLRNSRIYPGQKLSVFGA
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PETIRRSSKPSSAPSIVYHKVRKGESLIRIAKRYKVSVSNLASWNNLGRRRTIYAGQKLK
CHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEE
IYGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLK
EEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
IYGRGSGSTSSSSSVSQTHIVRRGETLGAIAKRYKVSVSNIASWNSLGRRRTIYPGQKLK
EEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
INGRTSGSTSGAARTYIVKRGDTLGAIASRYKVSVSKIVSWNNLGKRRLIYPGQKLKVSS
ECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]