Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 194334164

GI number: 194334164

Start: 1475749

End: 1476462

Strand: Reverse

Name: truA [H]

Synonym: Paes_1355

Alternate gene names: 194334164

Gene position: 1476462-1475749 (Counterclockwise)

Preceding gene: 194334172

Following gene: 194334163

Centisome position: 58.75

GC content: 52.66

Gene sequence:

>714_bases
ATGATTATCGAATACGACGGGACAGACTTTGCGGGGTGGCAGCGGCAGGATGGTGCTGTTTCGACCGTACAGGGTGAAAT
CGAAGCTGTTCTTCGGAGGATACTTCAGGAGGCGGTTTCTATCACAGGGGCCGGACGAACCGACAAGGGCGTTCATGCGA
TGGGTCAGGCGGCATCGTTCATGACCGGCAGCTCAATGGAAGCGGGCCGACTGTGCCATTCGTTGAATTCGCTCTTGCCT
TCATCGATCAGGATCATGTCCCTCATTGATGTTGATGGTGATTTTCATGCGCGTTACAGCGCTCTTTCGAGACAGTACCG
TTATATGCTCAGGCAGCGCGAGTCTGCAATTTTTCGCCGATTCAGCGGTTATGATCGAGCTTCTCATGATCTCGATCAGC
TCAACAGCAGGGCATCGGTGTTGCTGGGACGTCATGATTTCAAGGCTTTTTCAAAAAGCGGTTCCGATACCCGGACGACA
TGCTGCACGGTCTCGGAAGCGCAATGGTTTCGTGAGAAAGAGTTCCTTGTCTTTCAGATATCGGCAAATCGTTTTCTTCG
CTCTATGGTGCGTTATCTGGTGAGCGCCACACTCCGGTTTTCCGGTGAAGAGATCAGTTATGCTCTTGTAAGCCGTCAGC
CGATCAAGGGGCTGCTTCCAGCTGATCCGGCTGGACTTTTTTTGTGGGAGATCGGCTATGCGTCTGCCGGGTAA

Upstream 100 bases:

>100_bases
CTTGTCTTCAGCTCGCTCACGACAATTAATAGAGCTGCCCTTGTGATTGTTTCATTGTTTTGCTGAAAGGAAAAGAGAGG
TGCGTATCAGAAACCTTAAG

Downstream 100 bases:

>100_bases
AGGACATTGATGCTTCAGTTGTAATAATCACTAACTCTGTTTATGTTCTTGAGACCGGCTTCAGGTCGATAGCCAGAACG
GTTCTGAGCTTCAATTTCTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIIEYDGTDFAGWQRQDGAVSTVQGEIEAVLRRILQEAVSITGAGRTDKGVHAMGQAASFMTGSSMEAGRLCHSLNSLLP
SSIRIMSLIDVDGDFHARYSALSRQYRYMLRQRESAIFRRFSGYDRASHDLDQLNSRASVLLGRHDFKAFSKSGSDTRTT
CCTVSEAQWFREKEFLVFQISANRFLRSMVRYLVSATLRFSGEEISYALVSRQPIKGLLPADPAGLFLWEIGYASAG

Sequences:

>Translated_237_residues
MIIEYDGTDFAGWQRQDGAVSTVQGEIEAVLRRILQEAVSITGAGRTDKGVHAMGQAASFMTGSSMEAGRLCHSLNSLLP
SSIRIMSLIDVDGDFHARYSALSRQYRYMLRQRESAIFRRFSGYDRASHDLDQLNSRASVLLGRHDFKAFSKSGSDTRTT
CCTVSEAQWFREKEFLVFQISANRFLRSMVRYLVSATLRFSGEEISYALVSRQPIKGLLPADPAGLFLWEIGYASAG
>Mature_237_residues
MIIEYDGTDFAGWQRQDGAVSTVQGEIEAVLRRILQEAVSITGAGRTDKGVHAMGQAASFMTGSSMEAGRLCHSLNSLLP
SSIRIMSLIDVDGDFHARYSALSRQYRYMLRQRESAIFRRFSGYDRASHDLDQLNSRASVLLGRHDFKAFSKSGSDTRTT
CCTVSEAQWFREKEFLVFQISANRFLRSMVRYLVSATLRFSGEEISYALVSRQPIKGLLPADPAGLFLWEIGYASAG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=279, Percent_Identity=31.1827956989247, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI199559785, Length=230, Percent_Identity=25.2173913043478, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788657, Length=239, Percent_Identity=30.5439330543933, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 26446; Mature: 26446

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEYDGTDFAGWQRQDGAVSTVQGEIEAVLRRILQEAVSITGAGRTDKGVHAMGQAASF
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
MTGSSMEAGRLCHSLNSLLPSSIRIMSLIDVDGDFHARYSALSRQYRYMLRQRESAIFRR
HCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSGYDRASHDLDQLNSRASVLLGRHDFKAFSKSGSDTRTTCCTVSEAQWFREKEFLVFQI
HCCCCCCCCCHHHHCCHHHHEECHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCCEEEEEE
SANRFLRSMVRYLVSATLRFSGEEISYALVSRQPIKGLLPADPAGLFLWEIGYASAG
CHHHHHHHHHHHHHHHHHHCCCCHHEEHHHCCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MIIEYDGTDFAGWQRQDGAVSTVQGEIEAVLRRILQEAVSITGAGRTDKGVHAMGQAASF
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
MTGSSMEAGRLCHSLNSLLPSSIRIMSLIDVDGDFHARYSALSRQYRYMLRQRESAIFRR
HCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSGYDRASHDLDQLNSRASVLLGRHDFKAFSKSGSDTRTTCCTVSEAQWFREKEFLVFQI
HCCCCCCCCCHHHHCCHHHHEECHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCCEEEEEE
SANRFLRSMVRYLVSATLRFSGEEISYALVSRQPIKGLLPADPAGLFLWEIGYASAG
CHHHHHHHHHHHHHHHHHHCCCCHHEEHHHCCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA