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The map label for this gene is cobD [H]

Identifier: 194334080

GI number: 194334080

Start: 1370615

End: 1371589

Strand: Reverse

Name: cobD [H]

Synonym: Paes_1267

Alternate gene names: 194334080

Gene position: 1371589-1370615 (Counterclockwise)

Preceding gene: 194334081

Following gene: 194334079

Centisome position: 54.58

GC content: 51.49

Gene sequence:

>975_bases
ATGATGTATCCAGTTGAATACCAGATTGCAGCTGCTTTTCTTGTTGACTTTCTTGCCGGTGATCCGCGTTTTCTGCCGCA
TCCTGTCAGGGCAATAGGGTGGCTGGCTGCTGCTACGGAGCGTATCCTTCGCTCGATTCGTTGTCTTCATCTCCGCATTG
CAGGAGTTATGGCTGTTATCATTGTCGTTGGAGGAACGGTTGCTCTGGCATATGCTTTTCTGTTGCTCTGCGGTTTTCTG
CATCCGCTGCTGCAGTTCACTGCAGGGATCCTCATCTTATATTTCAGTATCGCATCAAGAGATCTGGCTGACCACGCTTA
TGCTGTACTGAGGCCTCTTGCCGTGAACGATATCGGCCAGGCCCGTCGGAGTGTCAGCATGATTGTCGGGCGAGATACCT
CCAGCCTTGATGAACAGGGAATCGCACAGGCAGCAGTTGAAAGTGTTGCCGAAAATACGGTGGACGGTGTTACGGCACCG
CTCTTTTATGCCCTGCTGTTCGGCCCGGTTGGAGCTCTTGCTTATAAAGCGGTCAACACGCTTGATTCAAGTTTCGGCTA
TAAAAATGAACGCTATCTTGAATTTGGATGGGCTTCGGCGCGTTTTGACGATCTTGTCAATTTTCTTCCGTCGCGTTTGA
CAGTGCCTTGTATTGCGGCTGCTGCCGCTCTGATGAGACTTCGTTACCGGGATGTGCTGCCATCTGTCCTGAGCACTGCA
GGGAAGCATACCAGTCCTAATGCCGGTTACCCTGAGGCGGCTTTTGCCGGAGCCCTCGGCGTGCGTTTCGGCGGTCCGAG
GAGTTATGGTGGAGTGGTGACAGATCTTCCGTATCTTGGCATTGCAGAGAATAGGCCGAACATTGATTCGATCAAACAGG
CGGTTGTTCTCATGCTGCTTTCTTCAGGTGTTTTTCTTGTGACGGGAGTTTTTTTGCGCCTTGTTGTTCAGGCGGCAATG
CTTTATCTGATGTGA

Upstream 100 bases:

>100_bases
AGGATAATTATTTCAGGTTTGCTATCCGTAAACCGGAAGAAAACGACTATATGCTCGATCAGCTTCAGCTTGTCGGCGGC
GAGGTCGGGATGGTTGAGAA

Downstream 100 bases:

>100_bases
TGAAAACTGGTGTAAACAATGATATACAGCCGACCGTACTCTTTGGCATCAGCAGTGTGTCGAGATGGCGTATCGTGCTT
TTTCTTCTCGCGGTTCCGCT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MMYPVEYQIAAAFLVDFLAGDPRFLPHPVRAIGWLAAATERILRSIRCLHLRIAGVMAVIIVVGGTVALAYAFLLLCGFL
HPLLQFTAGILILYFSIASRDLADHAYAVLRPLAVNDIGQARRSVSMIVGRDTSSLDEQGIAQAAVESVAENTVDGVTAP
LFYALLFGPVGALAYKAVNTLDSSFGYKNERYLEFGWASARFDDLVNFLPSRLTVPCIAAAAALMRLRYRDVLPSVLSTA
GKHTSPNAGYPEAAFAGALGVRFGGPRSYGGVVTDLPYLGIAENRPNIDSIKQAVVLMLLSSGVFLVTGVFLRLVVQAAM
LYLM

Sequences:

>Translated_324_residues
MMYPVEYQIAAAFLVDFLAGDPRFLPHPVRAIGWLAAATERILRSIRCLHLRIAGVMAVIIVVGGTVALAYAFLLLCGFL
HPLLQFTAGILILYFSIASRDLADHAYAVLRPLAVNDIGQARRSVSMIVGRDTSSLDEQGIAQAAVESVAENTVDGVTAP
LFYALLFGPVGALAYKAVNTLDSSFGYKNERYLEFGWASARFDDLVNFLPSRLTVPCIAAAAALMRLRYRDVLPSVLSTA
GKHTSPNAGYPEAAFAGALGVRFGGPRSYGGVVTDLPYLGIAENRPNIDSIKQAVVLMLLSSGVFLVTGVFLRLVVQAAM
LYLM
>Mature_324_residues
MMYPVEYQIAAAFLVDFLAGDPRFLPHPVRAIGWLAAATERILRSIRCLHLRIAGVMAVIIVVGGTVALAYAFLLLCGFL
HPLLQFTAGILILYFSIASRDLADHAYAVLRPLAVNDIGQARRSVSMIVGRDTSSLDEQGIAQAAVESVAENTVDGVTAP
LFYALLFGPVGALAYKAVNTLDSSFGYKNERYLEFGWASARFDDLVNFLPSRLTVPCIAAAAALMRLRYRDVLPSVLSTA
GKHTSPNAGYPEAAFAGALGVRFGGPRSYGGVVTDLPYLGIAENRPNIDSIKQAVVLMLLSSGVFLVTGVFLRLVVQAAM
LYLM

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34847; Mature: 34847

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYPVEYQIAAAFLVDFLAGDPRFLPHPVRAIGWLAAATERILRSIRCLHLRIAGVMAVI
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVVGGTVALAYAFLLLCGFLHPLLQFTAGILILYFSIASRDLADHAYAVLRPLAVNDIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRSVSMIVGRDTSSLDEQGIAQAAVESVAENTVDGVTAPLFYALLFGPVGALAYKAVNT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSSFGYKNERYLEFGWASARFDDLVNFLPSRLTVPCIAAAAALMRLRYRDVLPSVLSTA
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKHTSPNAGYPEAAFAGALGVRFGGPRSYGGVVTDLPYLGIAENRPNIDSIKQAVVLMLL
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
SSGVFLVTGVFLRLVVQAAMLYLM
HCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MMYPVEYQIAAAFLVDFLAGDPRFLPHPVRAIGWLAAATERILRSIRCLHLRIAGVMAVI
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVVGGTVALAYAFLLLCGFLHPLLQFTAGILILYFSIASRDLADHAYAVLRPLAVNDIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRSVSMIVGRDTSSLDEQGIAQAAVESVAENTVDGVTAPLFYALLFGPVGALAYKAVNT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSSFGYKNERYLEFGWASARFDDLVNFLPSRLTVPCIAAAAALMRLRYRDVLPSVLSTA
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKHTSPNAGYPEAAFAGALGVRFGGPRSYGGVVTDLPYLGIAENRPNIDSIKQAVVLMLL
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
SSGVFLVTGVFLRLVVQAAMLYLM
HCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]