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The map label for this gene is gra-orf5 [H]

Identifier: 194333969

GI number: 194333969

Start: 1254794

End: 1255633

Strand: Reverse

Name: gra-orf5 [H]

Synonym: Paes_1153

Alternate gene names: 194333969

Gene position: 1255633-1254794 (Counterclockwise)

Preceding gene: 194333970

Following gene: 194333964

Centisome position: 49.97

GC content: 51.19

Gene sequence:

>840_bases
ATGATGGATGATGCTAAAATAGAAAATCTCGGCATTGTCATTACCGGGGGGAGCAGAGGGCTTGGTTATGCACTTGCAAA
AGGGTTCCTCAATGTCGGCGACAGGGTCGTGATTTGCGCCAGGAATCCGGAAGGGGTGGATAGTGCGGTTTCATCACTTC
TGCAAAGTGTTCCGGACGGGAAGATTTTCGGTATATCGTGCGATGTGACGAATCCCGAGGATGCCTGTCGTCTCGCATCG
TTTGCGGTATCCGTTCTTGGTTCAGTTGACCGCTGGCTGAACAATGCCGGAACGTCAGGGTATCGCAAACGCCCCTTATG
GGAACTTGACGAGCGCGATTTGCTGGAGACGTGTACCACCAATCTCTATGGTTCTATGCTGATGAGCAAAACCGCTGTAG
GGATTATGCGTTCGCAGCCCGTAAAAGCCACTGAGGTATACCATATTTTTAATTTTGGTTTTACCTCCTTTGGGGCAAGG
TTTTCGCGTTCACCTGTCCCGCATAAGGCATCGAAAACAGGGGTAGCTGCAATTACCCGCCATCTTTCCAGGGAGATAAG
GCAAGCGGGAATCTCGGGAATCGGGGTCCATGAAGTCAGTCCGGGCCTTGTCCTGACTGATCTGCTGTTGCGGGATACCG
ATGACGAGACAAGGGCTTTTCTTCAGATAACGGCTGATACTCCCGAGGTTGTCGCACAAAGACTTGTTCCGAAAATAAGG
GGGGTCAAGGGGAGCAATTCGCTTATTCGATCGCAATCACTGCTAAGGATGGCGGGCAGCATGGCGATGAGAGTGATATC
AGCGCGATTCAGCCCAAAGCGACATCGAGAATCATCATAA

Upstream 100 bases:

>100_bases
TAGTATCAGGTCGTGTGTTTAACCTGTATGTCTGCTGTTCTCTGTGGTTGGCTACAGGGCTTTGCTTTAAGTCGATAAGC
TTCAGTTATGCGTGTAAATA

Downstream 100 bases:

>100_bases
CCGAAAAACCGAGCATAGTAGTCATCGTGACAAGATCGATGTTTTCATATTTTCGCTGTGATTCCGGTATCAGCTCTTCA
ACGACGACAAAAATCATGGC

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: ORF5 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MMDDAKIENLGIVITGGSRGLGYALAKGFLNVGDRVVICARNPEGVDSAVSSLLQSVPDGKIFGISCDVTNPEDACRLAS
FAVSVLGSVDRWLNNAGTSGYRKRPLWELDERDLLETCTTNLYGSMLMSKTAVGIMRSQPVKATEVYHIFNFGFTSFGAR
FSRSPVPHKASKTGVAAITRHLSREIRQAGISGIGVHEVSPGLVLTDLLLRDTDDETRAFLQITADTPEVVAQRLVPKIR
GVKGSNSLIRSQSLLRMAGSMAMRVISARFSPKRHRESS

Sequences:

>Translated_279_residues
MMDDAKIENLGIVITGGSRGLGYALAKGFLNVGDRVVICARNPEGVDSAVSSLLQSVPDGKIFGISCDVTNPEDACRLAS
FAVSVLGSVDRWLNNAGTSGYRKRPLWELDERDLLETCTTNLYGSMLMSKTAVGIMRSQPVKATEVYHIFNFGFTSFGAR
FSRSPVPHKASKTGVAAITRHLSREIRQAGISGIGVHEVSPGLVLTDLLLRDTDDETRAFLQITADTPEVVAQRLVPKIR
GVKGSNSLIRSQSLLRMAGSMAMRVISARFSPKRHRESS
>Mature_279_residues
MMDDAKIENLGIVITGGSRGLGYALAKGFLNVGDRVVICARNPEGVDSAVSSLLQSVPDGKIFGISCDVTNPEDACRLAS
FAVSVLGSVDRWLNNAGTSGYRKRPLWELDERDLLETCTTNLYGSMLMSKTAVGIMRSQPVKATEVYHIFNFGFTSFGAR
FSRSPVPHKASKTGVAAITRHLSREIRQAGISGIGVHEVSPGLVLTDLLLRDTDDETRAFLQITADTPEVVAQRLVPKIR
GVKGSNSLIRSQSLLRMAGSMAMRVISARFSPKRHRESS

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=203, Percent_Identity=31.5270935960591, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI109715829, Length=241, Percent_Identity=29.045643153527, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI19923817, Length=223, Percent_Identity=23.3183856502242, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24641232, Length=252, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355319, Length=200, Percent_Identity=27.5, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 30226; Mature: 30226

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDDAKIENLGIVITGGSRGLGYALAKGFLNVGDRVVICARNPEGVDSAVSSLLQSVPDG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
KIFGISCDVTNPEDACRLASFAVSVLGSVDRWLNNAGTSGYRKRPLWELDERDLLETCTT
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NLYGSMLMSKTAVGIMRSQPVKATEVYHIFNFGFTSFGARFSRSPVPHKASKTGVAAITR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCHHCCCCCCCCCCHHHHHHHHH
HLSREIRQAGISGIGVHEVSPGLVLTDLLLRDTDDETRAFLQITADTPEVVAQRLVPKIR
HHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHHHHHHHC
GVKGSNSLIRSQSLLRMAGSMAMRVISARFSPKRHRESS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMDDAKIENLGIVITGGSRGLGYALAKGFLNVGDRVVICARNPEGVDSAVSSLLQSVPDG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
KIFGISCDVTNPEDACRLASFAVSVLGSVDRWLNNAGTSGYRKRPLWELDERDLLETCTT
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NLYGSMLMSKTAVGIMRSQPVKATEVYHIFNFGFTSFGARFSRSPVPHKASKTGVAAITR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCHHCCCCCCCCCCHHHHHHHHH
HLSREIRQAGISGIGVHEVSPGLVLTDLLLRDTDDETRAFLQITADTPEVVAQRLVPKIR
HHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHHHHHHHC
GVKGSNSLIRSQSLLRMAGSMAMRVISARFSPKRHRESS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 7476861; 9831526 [H]