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The map label for this gene is appA [H]

Identifier: 193212225

GI number: 193212225

Start: 596631

End: 598406

Strand: Direct

Name: appA [H]

Synonym: Cpar_0556

Alternate gene names: 193212225

Gene position: 596631-598406 (Clockwise)

Preceding gene: 193212224

Following gene: 193212226

Centisome position: 26.06

GC content: 56.98

Gene sequence:

>1776_bases
ATGAAATTGAATAAGGCGTTTCATTCACTTTTTCCTGTCGTTCTGCTTGCTGCGGTGACGTTGTTGTCGGCCTGCTCGAA
GTCAGGTGGCAAAAATGCAGCTCTCTCGGCGGCGGCCAGGGATACCACGCTGGTCATCGGCATGCTCGGTGACGCCGATT
ACCTCAATCCGGTGATCGGTGCTTCGTTGACCTCGAGTGAGATTAGCGGCCTGATCTATCCGGGGTTGCTGCAGGGCGAG
TTCGATACCAAAACCGGCCTGCTCAATTACCTTGCTCTTGAAAAGCGGTTACGCCCATCGTCCGGTCCGGATGACAAATC
GCCGAAAGCTGCGCTGGCCAGAACCTGGTCCATGTCGCCCGACCACCGCTCGATCACCTACATTCTCAGGGATGACGCGA
AATGGGCCGACGGCCAGCCGATCACGTCGCGCGATTTCAAGTTTACCTACAAGTTGTACGGTAACCCGATCATTGCCAGC
CCGCGCCAGCAGTTCCTGGCCGAGCTTGTCGGCGCGGATAAGGGGACGGTCGATTTCGACAAAGCCATCGAAACGCCGAA
CGACACCACGCTGGTGTTTCATTTTTACAAGCCGGTGCCGGAGCATCTTGCCCTGTTCCACACCTCGCTCACGCCGGTGC
CGGAACATCTCTGGAAAGATGTAAAGCCCGAAGAGTTCCGCGAGTCGAAGCTCAACCAGCAGCCTGTGGGTGCGGGGCCG
TATCGCCTTGCGGAGTGGAACAAGCAGCAGTCGCTGGCGCTTTCGTCGAGTCCTTCGTGCAATCTGCCCAAGCCGGGCAA
TATCAAGCGCATCGTCTGGCGCATCGTGCCCGACTACACTGTCAGGCTCGCCCAGCTTCAGACGGGGGATGTCGATGTGG
TCGAAAGCGTCAAACCCGAGGATTTCGACGCGGTGCAGAAAGCCAATCCCGATGTGGAGGTCAAAACGGTCGGGCTCCGG
GTCTATGATTACGTCGGATGGATGAACATGGATGGTGATTATTACAACAAGACCGGCAAAGTTCGCCCGCATCCGCTTTT
CGGTGATCCGAAGGTCAGGCGTGCTCTGACCATGGCCATCGATCGCCAGTCAATCATTGATGGCTACCTTGGCGAGTATG
GTGTGTTGGCCAAAACCGATATTTCGCCCTCGCTCAAGTGGGCCTTCGACGATTCGATCAAGCCGTGGCCCTACGATCCG
ACTCAGGCCTCCAGACTGCTTGCCGGTGCCGGCTGGGTTCCGGGGCCCGACGGCATTCTGCAGAAGGATGGTCGCAGGTT
CAGCTTCGTGCTCTACACTAACGCCGGAAACGCTCGCCGCAACTACGCGGCGACGATCATCCAGCAGAACCTGAAGGAGA
TCGGCATCGACTGCAAAATTGAAATTCAAGAGTCGAACGTCTTTTTCCAGAACCTTCAGGAGCGCAAACTTGATGCCTGG
ATGGCTGGCTGGTCGATCGGTCTTGAAATCGATCCGCTCGATGTCTGGGGTTCCGATCTTGAGAAGAGCCGTTTCAATTT
CCCCGGCTACATCAATCCGAGGATCGACAAGCTCTGCGAGCTGGCCAAGGAGAAGATGCGCACCGAGGACGCCCGCCCCT
ACTGGGTCGAGTACCAGCGCATTCTGCACGAGGATCAGCCCATAACCTTCCTCTACTGGATGCGTGAGACGCAGGGCTTC
AACAAGCGGATTCAGGGCGAGAAGCTCAACATCTCAGGCAGTTTCTACAATATCGACGACTGGACTTTGAGGCCGTCGTC
ATCGATTGCGCCGTAA

Upstream 100 bases:

>100_bases
GTGGCTGCCGAGGCACTGACCGAGAGAATCAGTAATGTTATGAACAAAAACCGTAGCGATTCGGCCAGTGGCCCTTTTAT
CATTCCCGAATATTATGACG

Downstream 100 bases:

>100_bases
CGGTATGCTGAAGTATATCCTGAAACGGCTGTTGATTGCCGTGCCGCTCGTGTTCGGGGTGCTGACCCTGACCTTTTTTA
TCATCCGGCTTGCGCCCGGC

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKLNKAFHSLFPVVLLAAVTLLSACSKSGGKNAALSAAARDTTLVIGMLGDADYLNPVIGASLTSSEISGLIYPGLLQGE
FDTKTGLLNYLALEKRLRPSSGPDDKSPKAALARTWSMSPDHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPIIAS
PRQQFLAELVGADKGTVDFDKAIETPNDTTLVFHFYKPVPEHLALFHTSLTPVPEHLWKDVKPEEFRESKLNQQPVGAGP
YRLAEWNKQQSLALSSSPSCNLPKPGNIKRIVWRIVPDYTVRLAQLQTGDVDVVESVKPEDFDAVQKANPDVEVKTVGLR
VYDYVGWMNMDGDYYNKTGKVRPHPLFGDPKVRRALTMAIDRQSIIDGYLGEYGVLAKTDISPSLKWAFDDSIKPWPYDP
TQASRLLAGAGWVPGPDGILQKDGRRFSFVLYTNAGNARRNYAATIIQQNLKEIGIDCKIEIQESNVFFQNLQERKLDAW
MAGWSIGLEIDPLDVWGSDLEKSRFNFPGYINPRIDKLCELAKEKMRTEDARPYWVEYQRILHEDQPITFLYWMRETQGF
NKRIQGEKLNISGSFYNIDDWTLRPSSSIAP

Sequences:

>Translated_591_residues
MKLNKAFHSLFPVVLLAAVTLLSACSKSGGKNAALSAAARDTTLVIGMLGDADYLNPVIGASLTSSEISGLIYPGLLQGE
FDTKTGLLNYLALEKRLRPSSGPDDKSPKAALARTWSMSPDHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPIIAS
PRQQFLAELVGADKGTVDFDKAIETPNDTTLVFHFYKPVPEHLALFHTSLTPVPEHLWKDVKPEEFRESKLNQQPVGAGP
YRLAEWNKQQSLALSSSPSCNLPKPGNIKRIVWRIVPDYTVRLAQLQTGDVDVVESVKPEDFDAVQKANPDVEVKTVGLR
VYDYVGWMNMDGDYYNKTGKVRPHPLFGDPKVRRALTMAIDRQSIIDGYLGEYGVLAKTDISPSLKWAFDDSIKPWPYDP
TQASRLLAGAGWVPGPDGILQKDGRRFSFVLYTNAGNARRNYAATIIQQNLKEIGIDCKIEIQESNVFFQNLQERKLDAW
MAGWSIGLEIDPLDVWGSDLEKSRFNFPGYINPRIDKLCELAKEKMRTEDARPYWVEYQRILHEDQPITFLYWMRETQGF
NKRIQGEKLNISGSFYNIDDWTLRPSSSIAP
>Mature_591_residues
MKLNKAFHSLFPVVLLAAVTLLSACSKSGGKNAALSAAARDTTLVIGMLGDADYLNPVIGASLTSSEISGLIYPGLLQGE
FDTKTGLLNYLALEKRLRPSSGPDDKSPKAALARTWSMSPDHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPIIAS
PRQQFLAELVGADKGTVDFDKAIETPNDTTLVFHFYKPVPEHLALFHTSLTPVPEHLWKDVKPEEFRESKLNQQPVGAGP
YRLAEWNKQQSLALSSSPSCNLPKPGNIKRIVWRIVPDYTVRLAQLQTGDVDVVESVKPEDFDAVQKANPDVEVKTVGLR
VYDYVGWMNMDGDYYNKTGKVRPHPLFGDPKVRRALTMAIDRQSIIDGYLGEYGVLAKTDISPSLKWAFDDSIKPWPYDP
TQASRLLAGAGWVPGPDGILQKDGRRFSFVLYTNAGNARRNYAATIIQQNLKEIGIDCKIEIQESNVFFQNLQERKLDAW
MAGWSIGLEIDPLDVWGSDLEKSRFNFPGYINPRIDKLCELAKEKMRTEDARPYWVEYQRILHEDQPITFLYWMRETQGF
NKRIQGEKLNISGSFYNIDDWTLRPSSSIAP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=392, Percent_Identity=26.2755102040816, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787762, Length=547, Percent_Identity=24.3144424131627, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789887, Length=371, Percent_Identity=27.7628032345013, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787052, Length=577, Percent_Identity=23.2235701906412, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1787495, Length=475, Percent_Identity=24, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081878, Length=456, Percent_Identity=23.2456140350877, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789397, Length=320, Percent_Identity=24.6875, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66506; Mature: 66506

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01159 WW_DOMAIN_1 ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKAFHSLFPVVLLAAVTLLSACSKSGGKNAALSAAARDTTLVIGMLGDADYLNPVIG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEECCCCHHHHHHHC
ASLTSSEISGLIYPGLLQGEFDTKTGLLNYLALEKRLRPSSGPDDKSPKAALARTWSMSP
CCCCHHHCCCEECCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCC
DHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPIIASPRQQFLAELVGADKGTVDFD
CCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEECCHHHHHHHHHHCCCCCCCCHH
KAIETPNDTTLVFHFYKPVPEHLALFHTSLTPVPEHLWKDVKPEEFRESKLNQQPVGAGP
HHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCC
YRLAEWNKQQSLALSSSPSCNLPKPGNIKRIVWRIVPDYTVRLAQLQTGDVDVVESVKPE
CHHHCCCCCCCEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHCCCCC
DFDAVQKANPDVEVKTVGLRVYDYVGWMNMDGDYYNKTGKVRPHPLFGDPKVRRALTMAI
CHHHHHHCCCCEEEEEEEEEEEEEECEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
DRQSIIDGYLGEYGVLAKTDISPSLKWAFDDSIKPWPYDPTQASRLLAGAGWVPGPDGIL
HHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHH
QKDGRRFSFVLYTNAGNARRNYAATIIQQNLKEIGIDCKIEIQESNVFFQNLQERKLDAW
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
MAGWSIGLEIDPLDVWGSDLEKSRFNFPGYINPRIDKLCELAKEKMRTEDARPYWVEYQR
HCCCEECEEECCHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ILHEDQPITFLYWMRETQGFNKRIQGEKLNISGSFYNIDDWTLRPSSSIAP
HHCCCCCEEEEEEEHHCCCCCCCCCCCEEEECCCEEECCCCEECCCCCCCC
>Mature Secondary Structure
MKLNKAFHSLFPVVLLAAVTLLSACSKSGGKNAALSAAARDTTLVIGMLGDADYLNPVIG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEECCCCHHHHHHHC
ASLTSSEISGLIYPGLLQGEFDTKTGLLNYLALEKRLRPSSGPDDKSPKAALARTWSMSP
CCCCHHHCCCEECCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCC
DHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPIIASPRQQFLAELVGADKGTVDFD
CCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEECCHHHHHHHHHHCCCCCCCCHH
KAIETPNDTTLVFHFYKPVPEHLALFHTSLTPVPEHLWKDVKPEEFRESKLNQQPVGAGP
HHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCC
YRLAEWNKQQSLALSSSPSCNLPKPGNIKRIVWRIVPDYTVRLAQLQTGDVDVVESVKPE
CHHHCCCCCCCEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHCCCCC
DFDAVQKANPDVEVKTVGLRVYDYVGWMNMDGDYYNKTGKVRPHPLFGDPKVRRALTMAI
CHHHHHHCCCCEEEEEEEEEEEEEECEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
DRQSIIDGYLGEYGVLAKTDISPSLKWAFDDSIKPWPYDPTQASRLLAGAGWVPGPDGIL
HHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHH
QKDGRRFSFVLYTNAGNARRNYAATIIQQNLKEIGIDCKIEIQESNVFFQNLQERKLDAW
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
MAGWSIGLEIDPLDVWGSDLEKSRFNFPGYINPRIDKLCELAKEKMRTEDARPYWVEYQR
HCCCEECEEECCHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ILHEDQPITFLYWMRETQGFNKRIQGEKLNISGSFYNIDDWTLRPSSSIAP
HHCCCCCEEEEEEEHHCCCCCCCCCCCEEEECCCEEECCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]