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The map label for this gene is clpB1 [H]

Identifier: 193214146

GI number: 193214146

Start: 496685

End: 498016

Strand: Direct

Name: clpB1 [H]

Synonym: Ctha_0427

Alternate gene names: 193214146

Gene position: 496685-498016 (Clockwise)

Preceding gene: 193214139

Following gene: 193214150

Centisome position: 15.08

GC content: 47.0

Gene sequence:

>1332_bases
ATGCCGGTGAAAGACACATTACATCAGGATATTGAGACGAGATTGGCTGAAATTGATCAGCAATTGGCAAATTTGCGCGA
GGAACAAGAGCGAATACAAGCAAAGTGGCAATCGCAAAAAGAGCTGATTATGAGCATCCGCAAGCTCAAGTCAGACATAG
AAGAAGCCAAAGTACAAGCGGAAGAGTTTGAGCGCCAGGGCAATTACGGCAAAGTGGCCGAACTGCGCTATGGAAAGATT
ACGCAGCTTGAAAAAGAGCTTGAGCAAGGCAAAAAAAAGGTGGAAGCGCTTCGCGCGTCTGGCAATATGTTGATGAAAGA
AGACATCGAGAGCAAAGATATTGCTGACGTCGTTTCGAAATGGACGGGCATTCCTGTTTCAAAAATGCTGCAATCCGAGC
GGCAGAAAATTTTAAACATCGAAGATGAATTGCGCAAACGCGTTATTGGACAGCGAGAAGCCATTCACGCTGTTAGCGAA
GCGGTCAAACGTGCACGCGCTGGCTTAAGCGATGAAAAGCGACCAATTGGCTCGTTTATCTTTCTTGGCACCACGGGCGT
TGGAAAAACTGAGCTTGCCAGAAGCCTTGCGGCTTATCTTTTCAATGATGAGGACGCCATGGTACGAATTGATATGAGCG
AATACATGGAAGCGTTTAATGTGAGCCGTTTGGTTGGCGCGCCTCCCGGATATGTCGGTTATGAAGAAGGCGGCCAGCTC
ACGGAAGCCGTTCGTCGCAAGCCGTTTTCGGTTGTGCTGCTGGATGAAATTGAGAAAGCACATCCCGATGTGTTCAATAT
TTTACTCCAAGTGCTCGACGACGGCAGGCTTACCGACAACAAAGGCCATACCGTGAATTTCAGAAACACCATTATCATTA
TGACCAGCAACCTTGGCTCGCATTTGATCCAAGAAGAAATGGCAAAGCTAACCGATGAAAATAAAGAGACGATTCTTCCT
GAGCTGCGCGCCAAGCTTTTTGATTTGATGCGCAAAAGCCTTCGGCCAGAATTTCTGAACCGTGTGGACGAAACGATTCT
CTTCACGCCGCTCACGCGCGAAGATCTCAAGCAAATCGTGGATATTCAATTTGAGCGCATTCGGCAAACGGCTCAGAAGC
AAAATATTTTGCTTCGCATCACGGATGAAGCCAAAAATTGGCTTGGGCAATTGGGTTACGACCCGCACTTTGGCGCGCGC
CCACTCAAACGGGTTATGCAGCGATATATCACAAACGAACTTGCCGAAAAAATCCTTGGCGGAGAAGTTTCAGATGGGGA
TTCCATTGAAATCGTGTTGGAAAATAACCAGTTGGTTTTTAGGAAAAATTAA

Upstream 100 bases:

>100_bases
GCTTGAACATGCTTGAACATATTTATCCTTTTTTCCGAATAACCTGAGTGGCGATTTTTGTTCTTAACTCTACATTTAAC
ATTAAAAATTGAACTAAACT

Downstream 100 bases:

>100_bases
CCCGCTGCAACAAGAAACCTAACAAAAAAGCCTGCTTCGTCTCAGCGATGAAGCAGGCTTTCGTTTTTCTTCAGCGAAAA
ACTTCTTTCTTAATTTTCTG

Product: AAA ATPase-2 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MPVKDTLHQDIETRLAEIDQQLANLREEQERIQAKWQSQKELIMSIRKLKSDIEEAKVQAEEFERQGNYGKVAELRYGKI
TQLEKELEQGKKKVEALRASGNMLMKEDIESKDIADVVSKWTGIPVSKMLQSERQKILNIEDELRKRVIGQREAIHAVSE
AVKRARAGLSDEKRPIGSFIFLGTTGVGKTELARSLAAYLFNDEDAMVRIDMSEYMEAFNVSRLVGAPPGYVGYEEGGQL
TEAVRRKPFSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGHTVNFRNTIIIMTSNLGSHLIQEEMAKLTDENKETILP
ELRAKLFDLMRKSLRPEFLNRVDETILFTPLTREDLKQIVDIQFERIRQTAQKQNILLRITDEAKNWLGQLGYDPHFGAR
PLKRVMQRYITNELAEKILGGEVSDGDSIEIVLENNQLVFRKN

Sequences:

>Translated_443_residues
MPVKDTLHQDIETRLAEIDQQLANLREEQERIQAKWQSQKELIMSIRKLKSDIEEAKVQAEEFERQGNYGKVAELRYGKI
TQLEKELEQGKKKVEALRASGNMLMKEDIESKDIADVVSKWTGIPVSKMLQSERQKILNIEDELRKRVIGQREAIHAVSE
AVKRARAGLSDEKRPIGSFIFLGTTGVGKTELARSLAAYLFNDEDAMVRIDMSEYMEAFNVSRLVGAPPGYVGYEEGGQL
TEAVRRKPFSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGHTVNFRNTIIIMTSNLGSHLIQEEMAKLTDENKETILP
ELRAKLFDLMRKSLRPEFLNRVDETILFTPLTREDLKQIVDIQFERIRQTAQKQNILLRITDEAKNWLGQLGYDPHFGAR
PLKRVMQRYITNELAEKILGGEVSDGDSIEIVLENNQLVFRKN
>Mature_442_residues
PVKDTLHQDIETRLAEIDQQLANLREEQERIQAKWQSQKELIMSIRKLKSDIEEAKVQAEEFERQGNYGKVAELRYGKIT
QLEKELEQGKKKVEALRASGNMLMKEDIESKDIADVVSKWTGIPVSKMLQSERQKILNIEDELRKRVIGQREAIHAVSEA
VKRARAGLSDEKRPIGSFIFLGTTGVGKTELARSLAAYLFNDEDAMVRIDMSEYMEAFNVSRLVGAPPGYVGYEEGGQLT
EAVRRKPFSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGHTVNFRNTIIIMTSNLGSHLIQEEMAKLTDENKETILPE
LRAKLFDLMRKSLRPEFLNRVDETILFTPLTREDLKQIVDIQFERIRQTAQKQNILLRITDEAKNWLGQLGYDPHFGARP
LKRVMQRYITNELAEKILGGEVSDGDSIEIVLENNQLVFRKN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=36.875, Blast_Score=212, Evalue=5e-55,
Organism=Escherichia coli, GI1788943, Length=426, Percent_Identity=49.0610328638498, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787109, Length=337, Percent_Identity=43.620178041543, Blast_Score=279, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=441, Percent_Identity=44.8979591836735, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323002, Length=425, Percent_Identity=39.5294117647059, Blast_Score=326, Evalue=5e-90,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR001270
- InterPro:   IPR019489 [H]

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 50772; Mature: 50641

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKDTLHQDIETRLAEIDQQLANLREEQERIQAKWQSQKELIMSIRKLKSDIEEAKVQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEFERQGNYGKVAELRYGKITQLEKELEQGKKKVEALRASGNMLMKEDIESKDIADVVSK
HHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHH
WTGIPVSKMLQSERQKILNIEDELRKRVIGQREAIHAVSEAVKRARAGLSDEKRPIGSFI
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
FLGTTGVGKTELARSLAAYLFNDEDAMVRIDMSEYMEAFNVSRLVGAPPGYVGYEEGGQL
EEECCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAVRRKPFSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGHTVNFRNTIIIMTSNLGS
HHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCHHH
HLIQEEMAKLTDENKETILPELRAKLFDLMRKSLRPEFLNRVDETILFTPLTREDLKQIV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHH
DIQFERIRQTAQKQNILLRITDEAKNWLGQLGYDPHFGARPLKRVMQRYITNELAEKILG
HHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GEVSDGDSIEIVLENNQLVFRKN
CCCCCCCCEEEEEECCEEEEECC
>Mature Secondary Structure 
PVKDTLHQDIETRLAEIDQQLANLREEQERIQAKWQSQKELIMSIRKLKSDIEEAKVQA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEFERQGNYGKVAELRYGKITQLEKELEQGKKKVEALRASGNMLMKEDIESKDIADVVSK
HHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHH
WTGIPVSKMLQSERQKILNIEDELRKRVIGQREAIHAVSEAVKRARAGLSDEKRPIGSFI
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
FLGTTGVGKTELARSLAAYLFNDEDAMVRIDMSEYMEAFNVSRLVGAPPGYVGYEEGGQL
EEECCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAVRRKPFSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGHTVNFRNTIIIMTSNLGS
HHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCHHH
HLIQEEMAKLTDENKETILPELRAKLFDLMRKSLRPEFLNRVDETILFTPLTREDLKQIV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHH
DIQFERIRQTAQKQNILLRITDEAKNWLGQLGYDPHFGARPLKRVMQRYITNELAEKILG
HHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GEVSDGDSIEIVLENNQLVFRKN
CCCCCCCCEEEEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]