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The map label for this gene is pdhB [H]

Identifier: 192291581

GI number: 192291581

Start: 3455458

End: 3456867

Strand: Reverse

Name: pdhB [H]

Synonym: Rpal_3208

Alternate gene names: 192291581

Gene position: 3456867-3455458 (Counterclockwise)

Preceding gene: 192291582

Following gene: 192291580

Centisome position: 60.18

GC content: 65.04

Gene sequence:

>1410_bases
ATGCCAATCCAAGTTCTGATGCCCGCGCTGTCGCCGACCATGGAGAAGGGCAACCTGTCGAAATGGCTGAAGAAGGAAGG
CGACAAAGTCAAAAGCGGTGATGTCATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCTGCCGACGAAG
GTACGCTCGGTAAGATCCTGATTCCCGAAGGTACTAACGACGTCGCGGTGAACACCCCGATCGCCACCATCCTGGGCGAC
GGCGAAAGCGCGGCCGATGCCGACAAGGCGTCCGACCCGACCGCACAGAGCAAGGCCTCGCAATCGGCGCCGCCGTCGGC
GGAGCCTGAGGCCGCGCAGGCGAAGTCCGCAGCGGCGCCCGCCCAGCACGCGCCCGAAGCACCGACGGTGTCGGCGGCTG
CTGATCCGGATATTCCTGCCGGCACCGAGATGGTGACGGTGACGATCCGCGAAGCGCTGCGCGACGCGATGGCCGAGGAA
ATGCGCCGCGATCCCGACGTGTTCGTGATGGGTGAAGAGGTCGCCGAGTATCAGGGCGCCTACAAGGTCACGCAGGGGTT
GTTGCAGGAGTTCGGCGACCGCCGCGTGATCGACACACCGATCACCGAACACGGTTTTGCCGGCGTCGGCGTCGGCGCCG
GCTTTGCCGGGCTGAAGCCGATCGTCGAGTTCATGACCTTCAACTTCGCCATGCAGGCGATCGACCAGATCATCAACTCG
GCGGCGAAGACGCTGTACATGTCGGGCGGTCAGCTCGGCTGCTCGATCGTGTTCCGCGGTCCGAACGGCGCCGCCTCGCG
CGTCGCCGCGCAGCACAGCCAGGACTACTCGGCCTGGTACGCGCAGATCCCGGGCCTCAAGGTGGTGGCGCCGTATTCGG
CCGCCGACGCCAAGGGCCTGCTCAAGGCCGCGATCCGTGATCCCAATCCGGTGATCTTCCTCGAGCACGAGATGCTGTAC
GGGCAGCACGGCGAAGTGCCGAAGCTCGACGACTACGTGATCCCGATCGGCAAGGCGCGCATCGTCCGCGAGGGTAAGGA
CGTCACGCTGATCTCGTGGTCGCACGGCATGACCTACACGCTGAAGGCCGCCGACGAGCTGGCGAAGGACGGCATCTCGG
CCGAAGTGATCGACCTGCGTACGCTGCGTCCGCTCGACACCGACACCATCATCGCCTCGGTGAAGAAGACCGGGCGCGCG
GTGACGATCGAAGAAGGCTGGCAGCAGAACGGCGTCGGCGCCGAACTCTCTGCCCGGATCATGGAGCATGCCTTCGATTA
CTTGGATGCGCCGGTGACTCGGGTGTCGGGCAAGGACGTGCCGATGCCGTACGCGGCCAATCTCGAAAAGCTGGCATTGC
CCAGCGTCGCCGAGGTGGTCGAGGCCGCCAAAGCCGTCTGCTACAGGTAA

Upstream 100 bases:

>100_bases
GAGCCCGATCCGTCGGAGGTGTACACCGATGTGTATCGCTGATCCCCGCCTTGCCGTTTCGCGGCTGCTTCCCGCCTGAC
TGAATGACTGCGGAGCCCCA

Downstream 100 bases:

>100_bases
TCCTCTCCAATGCCTCGGCCAAATTGGACCATTAGGATTGGAAAGAAGGACAATGTCCTGGCTGAGTTCGTGGTTCCTAA
ATACAGGCTTGATCGTGGTT

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPIQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLGKILIPEGTNDVAVNTPIATILGD
GESAADADKASDPTAQSKASQSAPPSAEPEAAQAKSAAAPAQHAPEAPTVSAAADPDIPAGTEMVTVTIREALRDAMAEE
MRRDPDVFVMGEEVAEYQGAYKVTQGLLQEFGDRRVIDTPITEHGFAGVGVGAGFAGLKPIVEFMTFNFAMQAIDQIINS
AAKTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPYSAADAKGLLKAAIRDPNPVIFLEHEMLY
GQHGEVPKLDDYVIPIGKARIVREGKDVTLISWSHGMTYTLKAADELAKDGISAEVIDLRTLRPLDTDTIIASVKKTGRA
VTIEEGWQQNGVGAELSARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR

Sequences:

>Translated_469_residues
MPIQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLGKILIPEGTNDVAVNTPIATILGD
GESAADADKASDPTAQSKASQSAPPSAEPEAAQAKSAAAPAQHAPEAPTVSAAADPDIPAGTEMVTVTIREALRDAMAEE
MRRDPDVFVMGEEVAEYQGAYKVTQGLLQEFGDRRVIDTPITEHGFAGVGVGAGFAGLKPIVEFMTFNFAMQAIDQIINS
AAKTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPYSAADAKGLLKAAIRDPNPVIFLEHEMLY
GQHGEVPKLDDYVIPIGKARIVREGKDVTLISWSHGMTYTLKAADELAKDGISAEVIDLRTLRPLDTDTIIASVKKTGRA
VTIEEGWQQNGVGAELSARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR
>Mature_468_residues
PIQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLGKILIPEGTNDVAVNTPIATILGDG
ESAADADKASDPTAQSKASQSAPPSAEPEAAQAKSAAAPAQHAPEAPTVSAAADPDIPAGTEMVTVTIREALRDAMAEEM
RRDPDVFVMGEEVAEYQGAYKVTQGLLQEFGDRRVIDTPITEHGFAGVGVGAGFAGLKPIVEFMTFNFAMQAIDQIINSA
AKTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPYSAADAKGLLKAAIRDPNPVIFLEHEMLYG
QHGEVPKLDDYVIPIGKARIVREGKDVTLISWSHGMTYTLKAADELAKDGISAEVIDLRTLRPLDTDTIIASVKKTGRAV
TIEEGWQQNGVGAELSARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=58.0246913580247, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=54.9382716049383, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI4557353, Length=333, Percent_Identity=36.3363363363363, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI34101272, Length=333, Percent_Identity=36.3363363363363, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=51.8518518518518, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=51.8518518518518, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI31711992, Length=90, Percent_Identity=52.2222222222222, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI260898739, Length=81, Percent_Identity=50.6172839506173, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI133778974, Length=241, Percent_Identity=29.4605809128631, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI225637463, Length=322, Percent_Identity=27.3291925465839, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI225637459, Length=322, Percent_Identity=27.3291925465839, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI225637461, Length=322, Percent_Identity=27.3291925465839, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=62.5386996904025, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17506935, Length=333, Percent_Identity=39.3393393393393, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17560088, Length=132, Percent_Identity=40.9090909090909, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17539652, Length=263, Percent_Identity=31.9391634980989, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=59.6330275229358, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324258, Length=134, Percent_Identity=51.4925373134328, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321632, Length=121, Percent_Identity=43.801652892562, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358145, Length=322, Percent_Identity=61.8012422360248, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24650940, Length=322, Percent_Identity=61.8012422360248, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=38.5579937304075, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=38.5579937304075, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=62.2222222222222, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=62.2222222222222, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI20129315, Length=91, Percent_Identity=53.8461538461538, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45551847, Length=270, Percent_Identity=30.7407407407407, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550715, Length=270, Percent_Identity=30.7407407407407, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645119, Length=270, Percent_Identity=30.7407407407407, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582497, Length=72, Percent_Identity=52.7777777777778, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666278, Length=332, Percent_Identity=26.2048192771084, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49788; Mature: 49657

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLGKIL
CCCEEEEHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCCEEEE
IPEGTNDVAVNTPIATILGDGESAADADKASDPTAQSKASQSAPPSAEPEAAQAKSAAAP
ECCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCC
AQHAPEAPTVSAAADPDIPAGTEMVTVTIREALRDAMAEEMRRDPDVFVMGEEVAEYQGA
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCH
YKVTQGLLQEFGDRRVIDTPITEHGFAGVGVGAGFAGLKPIVEFMTFNFAMQAIDQIINS
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPYSAADAKGL
HHHEEEECCCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHH
LKAAIRDPNPVIFLEHEMLYGQHGEVPKLDDYVIPIGKARIVREGKDVTLISWSHGMTYT
HHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCEEECCCCEEEEECCCCEEEEEECCCCEEE
LKAADELAKDGISAEVIDLRTLRPLDTDTIIASVKKTGRAVTIEEGWQQNGVGAELSARI
EHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHH
MEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR
HHHHHHHHCCCHHCCCCCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIQVLMPALSPTMEKGNLSKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLGKIL
CCEEEEHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCCEEEE
IPEGTNDVAVNTPIATILGDGESAADADKASDPTAQSKASQSAPPSAEPEAAQAKSAAAP
ECCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCC
AQHAPEAPTVSAAADPDIPAGTEMVTVTIREALRDAMAEEMRRDPDVFVMGEEVAEYQGA
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCH
YKVTQGLLQEFGDRRVIDTPITEHGFAGVGVGAGFAGLKPIVEFMTFNFAMQAIDQIINS
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKTLYMSGGQLGCSIVFRGPNGAASRVAAQHSQDYSAWYAQIPGLKVVAPYSAADAKGL
HHHEEEECCCCEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHH
LKAAIRDPNPVIFLEHEMLYGQHGEVPKLDDYVIPIGKARIVREGKDVTLISWSHGMTYT
HHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCEEECCCCEEEEECCCCEEEEEECCCCEEE
LKAADELAKDGISAEVIDLRTLRPLDTDTIIASVKKTGRAVTIEEGWQQNGVGAELSARI
EHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHH
MEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSVAEVVEAAKAVCYR
HHHHHHHHCCCHHCCCCCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]