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The map label for this gene is pdhA [H]

Identifier: 192291582

GI number: 192291582

Start: 3456926

End: 3457960

Strand: Reverse

Name: pdhA [H]

Synonym: Rpal_3209

Alternate gene names: 192291582

Gene position: 3457960-3456926 (Counterclockwise)

Preceding gene: 192291583

Following gene: 192291581

Centisome position: 60.2

GC content: 62.51

Gene sequence:

>1035_bases
ATGGCCGCACCCAAGAAGAGCGCCGCGAAGGAAACAACACAGGACAAGGCCGGAGGAGCGTCACCATCGAATGTGCCGCC
TTTCACCAAGGAGCAGGAGCTCGGCGCTTTTCGTGAAATGCTGCTGATCCGCCGCTTCGAAGAGAAGGCCGGTCAGCTCT
ACGGCATGGGTGCGATCGGCGGATTCTGCCACCTCTACATCGGCCAGGAAGCCGTCGTCGTCGGCATGCAGATGGCGCTT
CGCGAAGGTGATCAGGTGATCACCGGCTATCGCGATCACGGCCACATGCTCGCCTGCGGCATGGACGCCAACGGCGTGAT
GGCCGAACTCACCGGGCGCCGCGGCGGCTACTCCAAAGGCAAGGGTGGCTCGATGCACATGTTCAGCCGGGAGAAGAGCT
TCTTCGGCGGACACGGCATCGTCGGCGCGCAGGTCTCGCTCGGCACCGGCATCGCCTTCGCAAACCGCTATCGCGGCGAC
GGACGCGTCTGCCTCGCTTATTTCGGCGACGGCGCCGCCAATCAGGGCCAGGTGTACGAGAGTTTCAACATGGCGGAGCT
GTGGAAGCTCCCTGTGGTGTATGTGATCGAGAACAACCGCTACGCGATGGGCACGTCGGTGACGCGTTCCTCGGCGCAGA
CCGATTTCTCTAAGCGCGGTGTGTCGTTCAATATTCCGGGCGAGCAGGTCGACGGTATGGACGTGCGTGCGGTGAAGGCC
GCCGGCGACAAGGCGGTTGCACATTGCCGCGCCGGAAACGGCCCGTTCATCCTCGAAATGCAGACCTATCGCTACCGCGG
TCACTCGATGTCGGACCCGGCGAAATATCGGTCGCGCGAAGAAGTCGACAAGATCCGCAACGATCAGGATCCGATCGAGC
AGGTGCGCAAGCGTCTGCTTGGGCTCGACATGACCGAAGACGATCTCAAGGCGATCGATGCCGAGGTCCGCAAGGTCGTC
AACGAGTCCGCGGACTTCGCTCAGCACGATCCCGAGCCCGATCCGTCGGAGGTGTACACCGATGTGTATCGCTGA

Upstream 100 bases:

>100_bases
GCACTGCGGCATATTGATTTGCGCGCAGCGACATAGCGATCCTTTCATGCCGCTATAAGTTACGATAGAGAGGCTCATCT
CATCTCTTATCGGAATCGCC

Downstream 100 bases:

>100_bases
TCCCCGCCTTGCCGTTTCGCGGCTGCTTCCCGCCTGACTGAATGACTGCGGAGCCCCAATGCCAATCCAAGTTCTGATGC
CCGCGCTGTCGCCGACCATG

Product: pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAAPKKSAAKETTQDKAGGASPSNVPPFTKEQELGAFREMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAVVVGMQMAL
REGDQVITGYRDHGHMLACGMDANGVMAELTGRRGGYSKGKGGSMHMFSREKSFFGGHGIVGAQVSLGTGIAFANRYRGD
GRVCLAYFGDGAANQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGVSFNIPGEQVDGMDVRAVKA
AGDKAVAHCRAGNGPFILEMQTYRYRGHSMSDPAKYRSREEVDKIRNDQDPIEQVRKRLLGLDMTEDDLKAIDAEVRKVV
NESADFAQHDPEPDPSEVYTDVYR

Sequences:

>Translated_344_residues
MAAPKKSAAKETTQDKAGGASPSNVPPFTKEQELGAFREMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAVVVGMQMAL
REGDQVITGYRDHGHMLACGMDANGVMAELTGRRGGYSKGKGGSMHMFSREKSFFGGHGIVGAQVSLGTGIAFANRYRGD
GRVCLAYFGDGAANQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGVSFNIPGEQVDGMDVRAVKA
AGDKAVAHCRAGNGPFILEMQTYRYRGHSMSDPAKYRSREEVDKIRNDQDPIEQVRKRLLGLDMTEDDLKAIDAEVRKVV
NESADFAQHDPEPDPSEVYTDVYR
>Mature_343_residues
AAPKKSAAKETTQDKAGGASPSNVPPFTKEQELGAFREMLLIRRFEEKAGQLYGMGAIGGFCHLYIGQEAVVVGMQMALR
EGDQVITGYRDHGHMLACGMDANGVMAELTGRRGGYSKGKGGSMHMFSREKSFFGGHGIVGAQVSLGTGIAFANRYRGDG
RVCLAYFGDGAANQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGVSFNIPGEQVDGMDVRAVKAA
GDKAVAHCRAGNGPFILEMQTYRYRGHSMSDPAKYRSREEVDKIRNDQDPIEQVRKRLLGLDMTEDDLKAIDAEVRKVVN
ESADFAQHDPEPDPSEVYTDVYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=324, Percent_Identity=51.2345679012346, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI291084742, Length=324, Percent_Identity=51.2345679012346, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI4505685, Length=324, Percent_Identity=51.2345679012346, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI291084744, Length=331, Percent_Identity=50.1510574018127, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI291084757, Length=324, Percent_Identity=45.3703703703704, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI11386135, Length=326, Percent_Identity=30.3680981595092, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI258645172, Length=326, Percent_Identity=30.9815950920245, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI32564172, Length=324, Percent_Identity=50.3086419753086, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17536047, Length=324, Percent_Identity=50.3086419753086, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI86563355, Length=325, Percent_Identity=27.3846153846154, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI86563357, Length=325, Percent_Identity=27.3846153846154, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=47.9623824451411, Blast_Score=301, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24639744, Length=324, Percent_Identity=50.9259259259259, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI28571106, Length=324, Percent_Identity=50.9259259259259, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24639740, Length=324, Percent_Identity=50.9259259259259, Blast_Score=330, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=52.2875816993464, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24639748, Length=324, Percent_Identity=50.9259259259259, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI21355903, Length=324, Percent_Identity=26.5432098765432, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 37697; Mature: 37566

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPKKSAAKETTQDKAGGASPSNVPPFTKEQELGAFREMLLIRRFEEKAGQLYGMGAIG
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHC
GFCHLYIGQEAVVVGMQMALREGDQVITGYRDHGHMLACGMDANGVMAELTGRRGGYSKG
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCEEEEECCCCCCCCCC
KGGSMHMFSREKSFFGGHGIVGAQVSLGTGIAFANRYRGDGRVCLAYFGDGAANQGQVYE
CCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHEECCCCCEEEEEECCCCCCCCCHHH
SFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGVSFNIPGEQVDGMDVRAVKA
CCCHHHHHCCCEEEEEECCCEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEC
AGDKAVAHCRAGNGPFILEMQTYRYRGHSMSDPAKYRSREEVDKIRNDQDPIEQVRKRLL
CCCHHEEEEECCCCCEEEEEEEEEECCCCCCCHHHHCCHHHHHHHCCCCCHHHHHHHHHH
GLDMTEDDLKAIDAEVRKVVNESADFAQHDPEPDPSEVYTDVYR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AAPKKSAAKETTQDKAGGASPSNVPPFTKEQELGAFREMLLIRRFEEKAGQLYGMGAIG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHC
GFCHLYIGQEAVVVGMQMALREGDQVITGYRDHGHMLACGMDANGVMAELTGRRGGYSKG
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCEEEEECCCCCCCCCC
KGGSMHMFSREKSFFGGHGIVGAQVSLGTGIAFANRYRGDGRVCLAYFGDGAANQGQVYE
CCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHEECCCCCEEEEEECCCCCCCCCHHH
SFNMAELWKLPVVYVIENNRYAMGTSVTRSSAQTDFSKRGVSFNIPGEQVDGMDVRAVKA
CCCHHHHHCCCEEEEEECCCEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEC
AGDKAVAHCRAGNGPFILEMQTYRYRGHSMSDPAKYRSREEVDKIRNDQDPIEQVRKRLL
CCCHHEEEEECCCCCEEEEEEEEEECCCCCCCHHHHCCHHHHHHHCCCCCHHHHHHHHHH
GLDMTEDDLKAIDAEVRKVVNESADFAQHDPEPDPSEVYTDVYR
CCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]