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The map label for this gene is pdhC [H]

Identifier: 192291579

GI number: 192291579

Start: 3453317

End: 3454723

Strand: Reverse

Name: pdhC [H]

Synonym: Rpal_3206

Alternate gene names: 192291579

Gene position: 3454723-3453317 (Counterclockwise)

Preceding gene: 192291580

Following gene: 192291578

Centisome position: 60.14

GC content: 66.03

Gene sequence:

>1407_bases
ATGCCGATCAACATTCTGATGCCCGCGCTGTCGCCGACCATGGAGAAGGGCAACCTTGCGAAGTGGCTGAAGAAGGAGGG
CGACAAGGTCAAGAGCGGCGACGTCATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCCGCCGACGAGG
GGACGCTCGCCAAGATCATCGTGCCCGAAGGCACCCAGGACGTGCCGGTCAACGACGTGATCGCGGTGCTGGCTGCGGAC
GGCGAGGACGTCAAGGCCGCGGGGGCAGGCTGGAAGGCCAGTGCCGGCGGTGCCTCCTCACCCCAGCCCTCTCCCCAGAG
GGAAGAGGGAGCCGGCCCCGCGGGCGGCAAGGCCGAGGCCAATTCGCACATACAGGACAAGGCTGACCAAAGGCCGACGC
CACAGCCGCCTTCACCTCTCCCTAACGGGGACAGGTCGCCGCCGCAGGCGGCGGGTGAGGGGGCTCCAGCGCCGGCGAAT
GGCCGCGTGTTCGCTTCGCCCTTGGCGCGGCGGCTGGCGAAGGATGCCGGCATCGATATCGCCCGGGTCACCGGCACCGG
ACCGCACGGCCGCGTCATTGCCCGCGACGTCGAGCAGGCCAAGAGCGGTGGCGGCCTGAAGGCAGCGGCTGCGGCGCCTG
CGGCCGGTCCCGCGATCGCGCCGGCGATGTCGGATCAGCAGATCCGCGCGCTGTATCCGGAAGGCTCCTATGAAGTCGTG
CCGCACGACGGCATGCGCCGGACCATTGCGCAAAGGTTGACGCAGTCGACCCAGACCATCCCGCATTTCTATCTGACGAT
CGACTGCAACCTCGATCGCCTGATGGCCGCGCGCGAGGACATCAACGCCGCCGCGCCGAAGGACAAGGACGGCAAGCCGG
CCTACAAGCTGTCGGTCAATGACTTCATCATCAAGGCGATGGCGATCGCCCTGCAGCGTATCCCCGACGCCAACGTATCA
TGGACCGAAGGCGGGATGTTGAAGCACAAGCATTCCGACATCGGCGTTGCGGTGGCGATGCCGGGCGGACTGATCACGCC
GATCATCCGCAGCGCCGAAACTCAGTCGCTGTCGTCGATCTCGGCGCAGATGAAGGATTTTGCCGCACGTGCGCGGGCCC
GCAAGCTGAAGCCTGAAGAGTACCAGGGCGGCACCACCGCGGTGTCCAATCTGGGGATGTTCGGAATCAAGGACTTCACC
GCCGTGATCAACCCGCCGCATGCTACGATCCTGGCGGTCGGCACCGGCGAGCAGCGCCCGATCGCCCGGGACGGCAAGAT
CGAGATCGCTACCATGATGAGTGTGACGCTGAGTTGCGATCACCGCGCCGTCGACGGTGCGCTCGGCGCCGAACTGATCG
GCGCCTTCAAGACGCTGATCGAAAATCCCGTGATGATGATGGTGTGA

Upstream 100 bases:

>100_bases
CGAAGCGCTCGAACGCATCGTCGAGATGTTCGAGGCCGAGCTGTCGCGCCCGACCGACACCGGCGCCACCACCGAACGGA
CGCAGTAAGGTTCTGTCCTC

Downstream 100 bases:

>100_bases
ACGTCTAACCCTCTCCCCTTGTGGGAGAGGTGGTCGCCACCTGTGGCCGGGTGAAGGGAAGAAGAGCGAGTAGTTATGTC
CGACACCTCCTTCGATGTCA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKIIVPEGTQDVPVNDVIAVLAAD
GEDVKAAGAGWKASAGGASSPQPSPQREEGAGPAGGKAEANSHIQDKADQRPTPQPPSPLPNGDRSPPQAAGEGAPAPAN
GRVFASPLARRLAKDAGIDIARVTGTGPHGRVIARDVEQAKSGGGLKAAAAAPAAGPAIAPAMSDQQIRALYPEGSYEVV
PHDGMRRTIAQRLTQSTQTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFIIKAMAIALQRIPDANVS
WTEGGMLKHKHSDIGVAVAMPGGLITPIIRSAETQSLSSISAQMKDFAARARARKLKPEEYQGGTTAVSNLGMFGIKDFT
AVINPPHATILAVGTGEQRPIARDGKIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV

Sequences:

>Translated_468_residues
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKIIVPEGTQDVPVNDVIAVLAAD
GEDVKAAGAGWKASAGGASSPQPSPQREEGAGPAGGKAEANSHIQDKADQRPTPQPPSPLPNGDRSPPQAAGEGAPAPAN
GRVFASPLARRLAKDAGIDIARVTGTGPHGRVIARDVEQAKSGGGLKAAAAAPAAGPAIAPAMSDQQIRALYPEGSYEVV
PHDGMRRTIAQRLTQSTQTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFIIKAMAIALQRIPDANVS
WTEGGMLKHKHSDIGVAVAMPGGLITPIIRSAETQSLSSISAQMKDFAARARARKLKPEEYQGGTTAVSNLGMFGIKDFT
AVINPPHATILAVGTGEQRPIARDGKIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV
>Mature_467_residues
PINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKIIVPEGTQDVPVNDVIAVLAADG
EDVKAAGAGWKASAGGASSPQPSPQREEGAGPAGGKAEANSHIQDKADQRPTPQPPSPLPNGDRSPPQAAGEGAPAPANG
RVFASPLARRLAKDAGIDIARVTGTGPHGRVIARDVEQAKSGGGLKAAAAAPAAGPAIAPAMSDQQIRALYPEGSYEVVP
HDGMRRTIAQRLTQSTQTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFIIKAMAIALQRIPDANVSW
TEGGMLKHKHSDIGVAVAMPGGLITPIIRSAETQSLSSISAQMKDFAARARARKLKPEEYQGGTTAVSNLGMFGIKDFTA
VINPPHATILAVGTGEQRPIARDGKIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=469, Percent_Identity=41.1513859275053, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI203098816, Length=496, Percent_Identity=36.6935483870968, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI203098753, Length=496, Percent_Identity=36.6935483870968, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=44.0993788819876, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI19923748, Length=263, Percent_Identity=31.1787072243346, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI110671329, Length=337, Percent_Identity=26.4094955489614, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786946, Length=468, Percent_Identity=27.7777777777778, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1786305, Length=313, Percent_Identity=32.5878594249201, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=467, Percent_Identity=43.254817987152, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=33.7579617834395, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=471, Percent_Identity=27.3885350318471, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=459, Percent_Identity=27.0152505446623, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324258, Length=471, Percent_Identity=41.4012738853503, Blast_Score=323, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6320352, Length=466, Percent_Identity=25.9656652360515, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321632, Length=322, Percent_Identity=27.9503105590062, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20129315, Length=470, Percent_Identity=39.7872340425532, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24582497, Length=459, Percent_Identity=39.2156862745098, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI18859875, Length=333, Percent_Identity=30.9309309309309, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645909, Length=239, Percent_Identity=31.7991631799163, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48869; Mature: 48738

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKII
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQDVPVNDVIAVLAADGEDVKAAGAGWKASAGGASSPQPSPQREEGAGPAGGKAEA
ECCCCCCCCHHHEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
NSHIQDKADQRPTPQPPSPLPNGDRSPPQAAGEGAPAPANGRVFASPLARRLAKDAGIDI
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCEE
ARVTGTGPHGRVIARDVEQAKSGGGLKAAAAAPAAGPAIAPAMSDQQIRALYPEGSYEVV
EEEECCCCCCCEEHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEC
PHDGMRRTIAQRLTQSTQTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVN
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHH
DFIIKAMAIALQRIPDANVSWTEGGMLKHKHSDIGVAVAMPGGLITPIIRSAETQSLSSI
HHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCHHHHHHHCCCHHHHHHH
SAQMKDFAARARARKLKPEEYQGGTTAVSNLGMFGIKDFTAVINPPHATILAVGTGEQRP
HHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCCCCCCHHHEEECCCCEEEEEEECCCCCC
IARDGKIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV
CCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
PINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKII
CCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQDVPVNDVIAVLAADGEDVKAAGAGWKASAGGASSPQPSPQREEGAGPAGGKAEA
ECCCCCCCCHHHEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
NSHIQDKADQRPTPQPPSPLPNGDRSPPQAAGEGAPAPANGRVFASPLARRLAKDAGIDI
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCEE
ARVTGTGPHGRVIARDVEQAKSGGGLKAAAAAPAAGPAIAPAMSDQQIRALYPEGSYEVV
EEEECCCCCCCEEHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEC
PHDGMRRTIAQRLTQSTQTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVN
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHH
DFIIKAMAIALQRIPDANVSWTEGGMLKHKHSDIGVAVAMPGGLITPIIRSAETQSLSSI
HHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCHHHHHHHCCCHHHHHHH
SAQMKDFAARARARKLKPEEYQGGTTAVSNLGMFGIKDFTAVINPPHATILAVGTGEQRP
HHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCCCCCCHHHEEECCCCEEEEEEECCCCCC
IARDGKIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV
CCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]