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The map label for this gene is lpd [H]

Identifier: 192291578

GI number: 192291578

Start: 3451820

End: 3453241

Strand: Reverse

Name: lpd [H]

Synonym: Rpal_3205

Alternate gene names: 192291578

Gene position: 3453241-3451820 (Counterclockwise)

Preceding gene: 192291579

Following gene: 192291577

Centisome position: 60.12

GC content: 64.7

Gene sequence:

>1422_bases
ATGTCCGACACCTCCTTCGATGTCATCATCATCGGTTCCGGCCCCGGCGGCTATGTCGCGGCGATCCGGGCGGCGCAGCT
CGGGTTCAAGACCGCGATTGTCGAGAAGTCTTATCTCGGCGGCATCTGCCTGAACTGGGGCTGCATCCCGACCAAGGCGC
TGCTGCGCTCGGCGGAAATCTATCACTACATGCAGCACGCCAAAGATTACGGGCTGTCGGCCGATAACATCTCGTTCGAT
CCGAAGGCGATCGTGCAGCGCTCGCGGGGGGTGTCGAAGCGGCTGAACGACGGCGTCGGCTTCCTGATGAAGAAGAACAA
GATCTCGATCATCTGGGGCGCGGCGACGATCGACGCGCCAGGCAAGCTGGCGGTCGCTGCCTCCAAGACCGAGGCGCCGA
AGGGCGCGCTGCCGCCGGGCAGCTATCAGGCCAAGCATATCATCGTCGCCACCGGGGCACGGCCGCGGGTGCTGCCAGGG
CTCGAGCCCGACAAGAAGCTGGTCTGGACCTATTTCGAAGCGATGGTGCCGGAGACCATGCCGAAGTCGCTGCTGGTGGT
CGGCTCAGGGGCGATCGGCATCGAGTTCGCCTCGTTCTTCCACACCATGGGCGCCAAGGTCACCGTGGTCGAAGTGCTGC
CGCAGATCTTGCCGGTCGAGGACGCCGAGATCGCAGCGCTCGCCCGCAAGCGGTTCGAGAAGCAGGGCATCAAGATCCTG
ACCGGCGCCAAGGTCACCAAGCTCGACAAGAAGGCCGACAGCGTGGTTGCCACCATCGATCCCGGCAACGGCAAGCCAGA
GACTGCGGAGTTCGACCGTGTGATCTCGGCGGTCGGCGTGGTCGGCAATGTCGAAAACCTCGGCCTGGACAAGCTCGGGG
TGAAGCTCGACCGCGGCACTATCGTCACCGACGGGCTCGGCCGCACCAACGTCCCCGGCCTCTATGCGATCGGGGACGTC
GCCGGGCCGCCAATGCTGGCGCACAAGGCAGAGCACGAGGGGGTGATCTGTGTCGAGGCCATCAAGGGCCTGCATCCGCA
TCCGCTCGACAAGAGCCTGATCCCCGGCTGCACCTACTGCCAGCCGCAGGTCGCCTCCGTGGGCCTGACAGAGGCCAAGG
CGAAGGAGCAGGGACGGGAGATCCGGGTCGGCCGCTTCCCGTTCACGGCCAACGGCAAGGCGATTGCGCTCGGCGAGGAC
CAGGGCCTGGTCAAGGTGATCTTCGACAAGAAGACCGGGCAGCTCTTGGGTGCGCATATGATCGGCGCCGAAGTCACCGA
GCTGATCCAGGGCTACGTGGTGGCGATGAACCTCGAGACCACCGAGGAAGAACTGATGCACACCGTCTTCCCGCACCCGA
CACTGTCGGAGATGATGAAGGAAGCCGTGCTCGACGCCTACGGCAAGGTGCTCAACGCCTGA

Upstream 100 bases:

>100_bases
AAATCCCGTGATGATGATGGTGTGAACGTCTAACCCTCTCCCCTTGTGGGAGAGGTGGTCGCCACCTGTGGCCGGGTGAA
GGGAAGAAGAGCGAGTAGTT

Downstream 100 bases:

>100_bases
CCCGAGCGGCGACTAAGTGCACCTGAAAAGGGTGTCCGCCAGGACACTCTTGTTGCTGTGTCGCAACAGTTCTGCATCAT
TTAACCGTCTTTGACGGTCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSDTSFDVIIIGSGPGGYVAAIRAAQLGFKTAIVEKSYLGGICLNWGCIPTKALLRSAEIYHYMQHAKDYGLSADNISFD
PKAIVQRSRGVSKRLNDGVGFLMKKNKISIIWGAATIDAPGKLAVAASKTEAPKGALPPGSYQAKHIIVATGARPRVLPG
LEPDKKLVWTYFEAMVPETMPKSLLVVGSGAIGIEFASFFHTMGAKVTVVEVLPQILPVEDAEIAALARKRFEKQGIKIL
TGAKVTKLDKKADSVVATIDPGNGKPETAEFDRVISAVGVVGNVENLGLDKLGVKLDRGTIVTDGLGRTNVPGLYAIGDV
AGPPMLAHKAEHEGVICVEAIKGLHPHPLDKSLIPGCTYCQPQVASVGLTEAKAKEQGREIRVGRFPFTANGKAIALGED
QGLVKVIFDKKTGQLLGAHMIGAEVTELIQGYVVAMNLETTEEELMHTVFPHPTLSEMMKEAVLDAYGKVLNA

Sequences:

>Translated_473_residues
MSDTSFDVIIIGSGPGGYVAAIRAAQLGFKTAIVEKSYLGGICLNWGCIPTKALLRSAEIYHYMQHAKDYGLSADNISFD
PKAIVQRSRGVSKRLNDGVGFLMKKNKISIIWGAATIDAPGKLAVAASKTEAPKGALPPGSYQAKHIIVATGARPRVLPG
LEPDKKLVWTYFEAMVPETMPKSLLVVGSGAIGIEFASFFHTMGAKVTVVEVLPQILPVEDAEIAALARKRFEKQGIKIL
TGAKVTKLDKKADSVVATIDPGNGKPETAEFDRVISAVGVVGNVENLGLDKLGVKLDRGTIVTDGLGRTNVPGLYAIGDV
AGPPMLAHKAEHEGVICVEAIKGLHPHPLDKSLIPGCTYCQPQVASVGLTEAKAKEQGREIRVGRFPFTANGKAIALGED
QGLVKVIFDKKTGQLLGAHMIGAEVTELIQGYVVAMNLETTEEELMHTVFPHPTLSEMMKEAVLDAYGKVLNA
>Mature_472_residues
SDTSFDVIIIGSGPGGYVAAIRAAQLGFKTAIVEKSYLGGICLNWGCIPTKALLRSAEIYHYMQHAKDYGLSADNISFDP
KAIVQRSRGVSKRLNDGVGFLMKKNKISIIWGAATIDAPGKLAVAASKTEAPKGALPPGSYQAKHIIVATGARPRVLPGL
EPDKKLVWTYFEAMVPETMPKSLLVVGSGAIGIEFASFFHTMGAKVTVVEVLPQILPVEDAEIAALARKRFEKQGIKILT
GAKVTKLDKKADSVVATIDPGNGKPETAEFDRVISAVGVVGNVENLGLDKLGVKLDRGTIVTDGLGRTNVPGLYAIGDVA
GPPMLAHKAEHEGVICVEAIKGLHPHPLDKSLIPGCTYCQPQVASVGLTEAKAKEQGREIRVGRFPFTANGKAIALGEDQ
GLVKVIFDKKTGQLLGAHMIGAEVTELIQGYVVAMNLETTEEELMHTVFPHPTLSEMMKEAVLDAYGKVLNA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=38.9473684210526, Blast_Score=308, Evalue=8e-84,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=29.5896328293737, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.801724137931, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI291045266, Length=466, Percent_Identity=26.1802575107296, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI33519430, Length=458, Percent_Identity=24.8908296943231, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI33519428, Length=458, Percent_Identity=24.8908296943231, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI33519426, Length=458, Percent_Identity=24.8908296943231, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=24.2489270386266, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI148277065, Length=466, Percent_Identity=24.2489270386266, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI291045268, Length=459, Percent_Identity=25.4901960784314, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=34.7161572052402, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082354, Length=480, Percent_Identity=27.9166666666667, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=28.9592760180996, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI87081717, Length=472, Percent_Identity=28.1779661016949, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=38.1856540084388, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=27.536231884058, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=26.4069264069264, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=462, Percent_Identity=26.4069264069264, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=499, Percent_Identity=24.6492985971944, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=185, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=37.9958246346555, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=28.2105263157895, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=28.9640591966173, Blast_Score=173, Evalue=7e-44,
Organism=Drosophila melanogaster, GI21358499, Length=474, Percent_Identity=39.662447257384, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI17737741, Length=476, Percent_Identity=26.2605042016807, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=27.3109243697479, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=27.3109243697479, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=27.3684210526316, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50321; Mature: 50190

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSFDVIIIGSGPGGYVAAIRAAQLGFKTAIVEKSYLGGICLNWGCIPTKALLRSAEI
CCCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
YHYMQHAKDYGLSADNISFDPKAIVQRSRGVSKRLNDGVGFLMKKNKISIIWGAATIDAP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCEEEEECCEEEEEEEEEEECCC
GKLAVAASKTEAPKGALPPGSYQAKHIIVATGARPRVLPGLEPDKKLVWTYFEAMVPETM
CCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
PKSLLVVGSGAIGIEFASFFHTMGAKVTVVEVLPQILPVEDAEIAALARKRFEKQGIKIL
CCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEE
TGAKVTKLDKKADSVVATIDPGNGKPETAEFDRVISAVGVVGNVENLGLDKLGVKLDRGT
ECCEEEHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHCEEEECCE
IVTDGLGRTNVPGLYAIGDVAGPPMLAHKAEHEGVICVEAIKGLHPHPLDKSLIPGCTYC
EEECCCCCCCCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCC
QPQVASVGLTEAKAKEQGREIRVGRFPFTANGKAIALGEDQGLVKVIFDKKTGQLLGAHM
CCCHHHCCCHHHHHHHCCCEEEEECCCEECCCCEEEEECCCCEEEEEEECCCCHHHHHHH
IGAEVTELIQGYVVAMNLETTEEELMHTVFPHPTLSEMMKEAVLDAYGKVLNA
HHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTSFDVIIIGSGPGGYVAAIRAAQLGFKTAIVEKSYLGGICLNWGCIPTKALLRSAEI
CCCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
YHYMQHAKDYGLSADNISFDPKAIVQRSRGVSKRLNDGVGFLMKKNKISIIWGAATIDAP
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCEEEEECCEEEEEEEEEEECCC
GKLAVAASKTEAPKGALPPGSYQAKHIIVATGARPRVLPGLEPDKKLVWTYFEAMVPETM
CCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
PKSLLVVGSGAIGIEFASFFHTMGAKVTVVEVLPQILPVEDAEIAALARKRFEKQGIKIL
CCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEE
TGAKVTKLDKKADSVVATIDPGNGKPETAEFDRVISAVGVVGNVENLGLDKLGVKLDRGT
ECCEEEHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHCEEEECCE
IVTDGLGRTNVPGLYAIGDVAGPPMLAHKAEHEGVICVEAIKGLHPHPLDKSLIPGCTYC
EEECCCCCCCCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCC
QPQVASVGLTEAKAKEQGREIRVGRFPFTANGKAIALGEDQGLVKVIFDKKTGQLLGAHM
CCCHHHCCCHHHHHHHCCCEEEEECCCEECCCCEEEEECCCCEEEEEEECCCCHHHHHHH
IGAEVTELIQGYVVAMNLETTEEELMHTVFPHPTLSEMMKEAVLDAYGKVLNA
HHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]