Click here to switch to the map view.

The map label for this gene is ispDF [H]

Identifier: 192291238

GI number: 192291238

Start: 3119017

End: 3120213

Strand: Reverse

Name: ispDF [H]

Synonym: Rpal_2860

Alternate gene names: 192291238

Gene position: 3120213-3119017 (Counterclockwise)

Preceding gene: 192291248

Following gene: 192291237

Centisome position: 54.32

GC content: 70.18

Gene sequence:

>1197_bases
ATGACGAACTCGCCCCGTACTGCTGCCATCATCGTCGCCGCCGGTCGCGGCCTGCGCGCCGGCGCGGGCGGCCCCAAGCA
ATACCGCACCCTCGCCGGCAGGCCGGTGATCGCGCGAGCGATGGAGCCGTTCTGCACCCATCCGGGCGTGATGGCGGTGC
AGCCGGTGACGAATCCCGACGACACCGAGATGTTCAACGCGGCAGTCGCCGGCCTGAATTTCCGCCCCGCGGTCGGCGGC
GGCGCAACCCGGCAGGCGTCGGTCCGCGCCGGCCTGGAGGCGCTGGCCGAGCTGAAGCCGGACATCGTGCTGATCCACGA
CGCGGCGCGTTGCTTCGTCACCCCGGAGCTGATCTCGCGCGCGATCACCGCGGCCGGCGCGACCGGCGCTGCGCTGCCTG
TCGTCGCCGTAACCGACACGATCAAGCAGGTCGACAGCAGCGGCGCGGTCGACGCGACGCCGGACCGCGCTAGCTTGCGG
ATCGCCCAGACCCCACAGGCGTTCCGTTTCGATGTGATCCTCGACGCCCACCGCCGCGCGGCCTCTGGCGGCCGGGACGA
TTTCACCGACGACGCCGCGATCGCCGAATGGGCGGGATTGACGGTGTCGACCTTCGAGGGCGATGCTAACAACATGAAGA
TGACCACCCCGGAAGATTTCGCGCGCGAGGAAAGCCGGCTGATGGCCGCACTCGGCGACATCCGCACCGGCACCGGCTAC
GACGTGCATGCGTTCGGCGAAGGCGACCACGTCTGGCTGTGTGGCCTGAAGGTGCCGCACACCCGCGGCTTCCTGGCGCA
TTCTGACGGCGACGTCGGCCTGCACGCGCTGGTCGACGCGATCCTCGGCGCGCTGGCGGACGGCGACATCGGCTCTCACT
TCCCGCCGACCGATCCGCAGTGGAAGGGCGCGGCCTCTGACAAGTTCCTCAAATACGCGATCGACCGCGTCACTGCGCGC
GGCGGCCGCGTCGCCAATCTCGAAGTGACGATGATCTGTGAGCGACCGAAGATCGGACCGCTGCGCGACGCGATGCGTCA
GCGCATCGCCGAGATCACCGGCGTGCCGGTGTCGCGCGTCGCGGTGAAGGCCACCACCAGCGAGAAGCTCGGCTTCACCG
GCCGTGAGGAAGGCATCGCGGCGACCGCATCCGCCACCATCCGGCTGCCCTGGGGCGCCGACGGACTGGCCGGCTGA

Upstream 100 bases:

>100_bases
TGCGGCGATGGAATCATGCTGCTTTCCTAGCCCGGCATGGTAAGGGCTGTGCACCGGCGCGACCAGCGCAGGCCATCCCA
ATTCCGTATCAATTGATCCG

Downstream 100 bases:

>100_bases
CGATGAGCAGCAACGACGCCCGCGCTCTCGCCCGCTCGTTGCTCGACCTCTGCCGTTCGCGCAAGCTGATGATCGCGACC
GCCGAGTCCTGCACCGGCGG

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTNSPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARAMEPFCTHPGVMAVQPVTNPDDTEMFNAAVAGLNFRPAVGG
GATRQASVRAGLEALAELKPDIVLIHDAARCFVTPELISRAITAAGATGAALPVVAVTDTIKQVDSSGAVDATPDRASLR
IAQTPQAFRFDVILDAHRRAASGGRDDFTDDAAIAEWAGLTVSTFEGDANNMKMTTPEDFAREESRLMAALGDIRTGTGY
DVHAFGEGDHVWLCGLKVPHTRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQWKGAASDKFLKYAIDRVTAR
GGRVANLEVTMICERPKIGPLRDAMRQRIAEITGVPVSRVAVKATTSEKLGFTGREEGIAATASATIRLPWGADGLAG

Sequences:

>Translated_398_residues
MTNSPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARAMEPFCTHPGVMAVQPVTNPDDTEMFNAAVAGLNFRPAVGG
GATRQASVRAGLEALAELKPDIVLIHDAARCFVTPELISRAITAAGATGAALPVVAVTDTIKQVDSSGAVDATPDRASLR
IAQTPQAFRFDVILDAHRRAASGGRDDFTDDAAIAEWAGLTVSTFEGDANNMKMTTPEDFAREESRLMAALGDIRTGTGY
DVHAFGEGDHVWLCGLKVPHTRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQWKGAASDKFLKYAIDRVTAR
GGRVANLEVTMICERPKIGPLRDAMRQRIAEITGVPVSRVAVKATTSEKLGFTGREEGIAATASATIRLPWGADGLAG
>Mature_397_residues
TNSPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARAMEPFCTHPGVMAVQPVTNPDDTEMFNAAVAGLNFRPAVGGG
ATRQASVRAGLEALAELKPDIVLIHDAARCFVTPELISRAITAAGATGAALPVVAVTDTIKQVDSSGAVDATPDRASLRI
AQTPQAFRFDVILDAHRRAASGGRDDFTDDAAIAEWAGLTVSTFEGDANNMKMTTPEDFAREESRLMAALGDIRTGTGYD
VHAFGEGDHVWLCGLKVPHTRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQWKGAASDKFLKYAIDRVTARG
GRVANLEVTMICERPKIGPLRDAMRQRIAEITGVPVSRVAVKATTSEKLGFTGREEGIAATASATIRLPWGADGLAG

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=30.1724137931034, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=52.5974025974026, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=33.1838565022422, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 41649; Mature: 41517

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARAMEPFCTHPGVMAVQPVTNPD
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCC
DTEMFNAAVAGLNFRPAVGGGATRQASVRAGLEALAELKPDIVLIHDAARCFVTPELISR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEECHHHHHH
AITAAGATGAALPVVAVTDTIKQVDSSGAVDATPDRASLRIAQTPQAFRFDVILDAHRRA
HHHHCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECHHHH
ASGGRDDFTDDAAIAEWAGLTVSTFEGDANNMKMTTPEDFAREESRLMAALGDIRTGTGY
CCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCC
DVHAFGEGDHVWLCGLKVPHTRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQ
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
WKGAASDKFLKYAIDRVTARGGRVANLEVTMICERPKIGPLRDAMRQRIAEITGVPVSRV
CCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHE
AVKATTSEKLGFTGREEGIAATASATIRLPWGADGLAG
EEEECCCHHCCCCCCCCCEEEECEEEEECCCCCCCCCC
>Mature Secondary Structure 
TNSPRTAAIIVAAGRGLRAGAGGPKQYRTLAGRPVIARAMEPFCTHPGVMAVQPVTNPD
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCC
DTEMFNAAVAGLNFRPAVGGGATRQASVRAGLEALAELKPDIVLIHDAARCFVTPELISR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEECHHHHHH
AITAAGATGAALPVVAVTDTIKQVDSSGAVDATPDRASLRIAQTPQAFRFDVILDAHRRA
HHHHCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECHHHH
ASGGRDDFTDDAAIAEWAGLTVSTFEGDANNMKMTTPEDFAREESRLMAALGDIRTGTGY
CCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCC
DVHAFGEGDHVWLCGLKVPHTRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQ
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
WKGAASDKFLKYAIDRVTARGGRVANLEVTMICERPKIGPLRDAMRQRIAEITGVPVSRV
CCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHE
AVKATTSEKLGFTGREEGIAATASATIRLPWGADGLAG
EEEECCCHHCCCCCCCCCEEEECEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA