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The map label for this gene is lip2 [H]

Identifier: 192291082

GI number: 192291082

Start: 2934967

End: 2935917

Strand: Reverse

Name: lip2 [H]

Synonym: Rpal_2703

Alternate gene names: 192291082

Gene position: 2935917-2934967 (Counterclockwise)

Preceding gene: 192291084

Following gene: 192291081

Centisome position: 51.11

GC content: 68.24

Gene sequence:

>951_bases
ATGCCCGCCAAGCTCGATCCCGATCCCGATGCCGCTGCCGTTTACAAGGCCTTCCAGGACGCCGGCCGCCCCGCCTACGA
GACACTGACCGCCGCCGAAGCGCGCGCCTACTACTCGGCAGCGCGGCTGGTCAGCAATCCGGATCCAGCCGACATGGCAT
CGGTTCGTTCGATCGCGATCCCCGGCCCGGCCGGCGATATCCCGGCGCGGCTCTACACCCCGAACAAGTTGCGCCAAGAC
GAGGGCCTAGCGCCGGCGCTGGTGTTCTTTCATGGCGGCGGCTGGGTGATCGGCGATCTCGATACCCACGACGTGGTGTG
CCGCGGCATCGCCCATGACGGCGAACTGCTGGTGATCTCGGTCGACTACCGGCTCGCACCCGAGCACAAATTCCCCGCTG
CCATCGACGACGCAATCGCCGCGACGCGCTGGATTGCCGACAATGCCAGGAAGCTCGGCATCGATCCCGAGCAGCTGAGC
GTCGGCGGCGACAGCGCCGGCGGCAATCTCTCCGCGGTGGTCGCGCTGCACGCCCGCGATCACGGTGGACCGCTGCTCGC
CGGCCAGGTGCTGATCTATCCCGCCACCGATTTCTCGATGCGGCATCCGTCGCACAGCGAGCCGGAGACTAGCGTGCTGT
TGACGCATTCGGTGATCCGCTGGTTTCGCGATCACTATCTGAGCGGCGCGCAGGACGCCGACGACTGGCGCGCCTCGCCG
GCACGCGCCGAAACCCTTGCGGGCCTGCCGCCCGCCTTCGTCATCACCGCCGGCGCCGATCCGCTGCGCGACGAAGGCGA
CGAATACGCCCGCCGCCTCGCCGATGCCGGCGTCCCGGTAGTGCACCGGACCTATCCGGGTCAATTCCACGGCTTCTTCA
CCATGGGCAAGCTGCTGCCGCAGGCCAACGTCGCGGTGCGCGAGATCGGCGACTGGCTGAAGGCGCTTTAG

Upstream 100 bases:

>100_bases
TTGCGAGGAACTCCTGAGATATTGGTTTCAAACCCCGGCGGGCCTGACCGTCGCGGAATAAGCAAGCCCCGGCACGAACC
GGGAAGCACGAGGAAGTCCC

Downstream 100 bases:

>100_bases
CCGCAGGCCGGCATTGACCGGCGTCAACGTGACCGCGCTCCGGCCGTCCGAGGATTTGCGCGGTCAACAGGGAGCAGCGG
CGCACGGCGCCGATCGTCAC

Product: alpha/beta hydrolase fold-3 domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPAKLDPDPDAAAVYKAFQDAGRPAYETLTAAEARAYYSAARLVSNPDPADMASVRSIAIPGPAGDIPARLYTPNKLRQD
EGLAPALVFFHGGGWVIGDLDTHDVVCRGIAHDGELLVISVDYRLAPEHKFPAAIDDAIAATRWIADNARKLGIDPEQLS
VGGDSAGGNLSAVVALHARDHGGPLLAGQVLIYPATDFSMRHPSHSEPETSVLLTHSVIRWFRDHYLSGAQDADDWRASP
ARAETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVVHRTYPGQFHGFFTMGKLLPQANVAVREIGDWLKAL

Sequences:

>Translated_316_residues
MPAKLDPDPDAAAVYKAFQDAGRPAYETLTAAEARAYYSAARLVSNPDPADMASVRSIAIPGPAGDIPARLYTPNKLRQD
EGLAPALVFFHGGGWVIGDLDTHDVVCRGIAHDGELLVISVDYRLAPEHKFPAAIDDAIAATRWIADNARKLGIDPEQLS
VGGDSAGGNLSAVVALHARDHGGPLLAGQVLIYPATDFSMRHPSHSEPETSVLLTHSVIRWFRDHYLSGAQDADDWRASP
ARAETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVVHRTYPGQFHGFFTMGKLLPQANVAVREIGDWLKAL
>Mature_315_residues
PAKLDPDPDAAAVYKAFQDAGRPAYETLTAAEARAYYSAARLVSNPDPADMASVRSIAIPGPAGDIPARLYTPNKLRQDE
GLAPALVFFHGGGWVIGDLDTHDVVCRGIAHDGELLVISVDYRLAPEHKFPAAIDDAIAATRWIADNARKLGIDPEQLSV
GGDSAGGNLSAVVALHARDHGGPLLAGQVLIYPATDFSMRHPSHSEPETSVLLTHSVIRWFRDHYLSGAQDADDWRASPA
RAETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVVHRTYPGQFHGFFTMGKLLPQANVAVREIGDWLKAL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=313, Percent_Identity=29.3929712460064, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI206597554, Length=287, Percent_Identity=30.6620209059233, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI157041239, Length=286, Percent_Identity=27.972027972028, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI68051721, Length=305, Percent_Identity=24.9180327868852, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI61966717, Length=320, Percent_Identity=28.125, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI226423947, Length=313, Percent_Identity=24.2811501597444, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI157041237, Length=251, Percent_Identity=25.8964143426295, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI21328446, Length=110, Percent_Identity=39.0909090909091, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786682, Length=293, Percent_Identity=30.3754266211604, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17567059, Length=277, Percent_Identity=32.8519855595668, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17540028, Length=134, Percent_Identity=40.2985074626866, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=129, Percent_Identity=38.7596899224806, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=111, Percent_Identity=40.5405405405405, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24656084, Length=114, Percent_Identity=37.719298245614, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656076, Length=114, Percent_Identity=37.719298245614, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130169, Length=114, Percent_Identity=37.719298245614, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33847; Mature: 33716

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLDPDPDAAAVYKAFQDAGRPAYETLTAAEARAYYSAARLVSNPDPADMASVRSIAI
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEC
PGPAGDIPARLYTPNKLRQDEGLAPALVFFHGGGWVIGDLDTHDVVCRGIAHDGELLVIS
CCCCCCCCCEEECCCHHCCCCCCCEEEEEEECCCEEEECCCCHHHHHEECCCCCCEEEEE
VDYRLAPEHKFPAAIDDAIAATRWIADNARKLGIDPEQLSVGGDSAGGNLSAVVALHARD
ECCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCCCEEEEEEEEECC
HGGPLLAGQVLIYPATDFSMRHPSHSEPETSVLLTHSVIRWFRDHYLSGAQDADDWRASP
CCCCEEECEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
ARAETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVVHRTYPGQFHGFFTMGKLLP
CHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCC
QANVAVREIGDWLKAL
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PAKLDPDPDAAAVYKAFQDAGRPAYETLTAAEARAYYSAARLVSNPDPADMASVRSIAI
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEC
PGPAGDIPARLYTPNKLRQDEGLAPALVFFHGGGWVIGDLDTHDVVCRGIAHDGELLVIS
CCCCCCCCCEEECCCHHCCCCCCCEEEEEEECCCEEEECCCCHHHHHEECCCCCCEEEEE
VDYRLAPEHKFPAAIDDAIAATRWIADNARKLGIDPEQLSVGGDSAGGNLSAVVALHARD
ECCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCCCEEEEEEEEECC
HGGPLLAGQVLIYPATDFSMRHPSHSEPETSVLLTHSVIRWFRDHYLSGAQDADDWRASP
CCCCEEECEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
ARAETLAGLPPAFVITAGADPLRDEGDEYARRLADAGVPVVHRTYPGQFHGFFTMGKLLP
CHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCC
QANVAVREIGDWLKAL
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]