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The map label for this gene is agaB [H]

Identifier: 192291081

GI number: 192291081

Start: 2933526

End: 2934953

Strand: Reverse

Name: agaB [H]

Synonym: Rpal_2702

Alternate gene names: 192291081

Gene position: 2934953-2933526 (Counterclockwise)

Preceding gene: 192291082

Following gene: 192291079

Centisome position: 51.1

GC content: 67.23

Gene sequence:

>1428_bases
TTGACCGGCGTCAACGTGACCGCGCTCCGGCCGTCCGAGGATTTGCGCGGTCAACAGGGAGCAGCGGCGCACGGCGCCGA
TCGTCACGACGGAATGACTGAACTTGCACGCACGCGCTGGGGCGGCCTTGCCGATCGCAAGGTCGACGCCAGCGGTTTCT
TCCGGACCGAACAGATCGACGGCGTATGGTGGTTCATCGATCCGGATGGCGGACGTTTTCTGTCGAAGGGCGTGGTCTCG
GTGCAGTTCGACCACGACAACGTTCAGGGCACGGATCGCCGGCCGTATCGCGAAGCCTGCACGCGTAAATACGGCAGCCG
CGAAGCGTGGCGACGCGTCGCGGCGGACCGCCTCACCTCCTGGGGCTTCAACACGCTCGGCGCCTGGTCGGAGCCGGAGA
TCGCACGCGCCGGCACCGTGCCCCTCGCCTCCGCCGCCGGCGTCGTCTATGTCGCGACCGCATTCGGCGAACAGCGCGGC
GGCTGGCCGCTGTGCGACATCTTCGAGCCGGAATTCGAACAGTTCGCCCGCGCGCACGCGCGTAAGGTCTGCACGCCGCA
AAGCGACGATCCGCATGTGCTCGGCTGGTTCACCGATAACGAGTTGCCATGGGGACCGGACTGGCGCGGGGAAAACGAAC
TGCTGCCCACCATCCTTCGCGCGATGGACGCGCCATTCTCGCGGCGCGCCGCGCTTGCGCTGCTGAAAAGCCGCTACGAC
AGCATCGATCAGTTCAATGCGGCGTGGCAATGCAAGCTGCCATCTTGGGACGATCTCGCCGCCACGCCGCTGCAGCCGCC
GCCGTTCAGCCGCAACTTCTTCAGCAACGACCGGGCGCAGGAGCACGATCCGATCGCGCGCGGTTACTTCGCCGATTGCG
ACGCCTTCGCCGGGCTGCTGGCCGAGCGCTACCTGTCGGTTTGCGCCACCGCGATCCGCGCTGCCGATCCAAATCACCTC
GTGCTCGGCGCCAGGTTCGCTTACGTGCCGCCGCGCGACGTCGTCGATGCTGCCGGCAGGTATTGCGACGTAATCAGCGT
CAATTGCTACGATCCGCTGCCGGACGCGGTAATCGACGGCTACGCCGAAACCGGGCGGCCGTGCCTGATCGGCGAATACT
CGTTCCGCGGCGACGATGCTGGACTCCCCAATACGCACGGCGCCGGTCCGCGGATGCCGACGCAGCGGGAGCGCGCGGCA
GGATTCACGCGCTACACCACCGCAGCGCTGCGCCATCCGGCGCTGATCGGCTATCACTGGTTCGTCCACGCCGACCAGCC
GGCGCAAGGCCGCTGGGACGGCGAGGACTCCAACTACGGCGTGGTGACGATCGAAGACGAGGTCTATCCCGAGCTGACCC
AGGCGATGACGGCGCTCAACGCCGAGGCCGAACGGATTCACGTCGAGACCGCGCCGGTGTTGGCCTGA

Upstream 100 bases:

>100_bases
TTCCACGGCTTCTTCACCATGGGCAAGCTGCTGCCGCAGGCCAACGTCGCGGTGCGCGAGATCGGCGACTGGCTGAAGGC
GCTTTAGCCGCAGGCCGGCA

Downstream 100 bases:

>100_bases
CCGGAGCCGCCGCGATCGTCACGCGGCCTTCGCGGCGCGGCGCAGCGCTTGCGGCGGGTGACCGAAGGCGCGGATGAAGG
CGCGGCGCATCCGCTCCGGA

Product: beta-agarase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTGVNVTALRPSEDLRGQQGAAAHGADRHDGMTELARTRWGGLADRKVDASGFFRTEQIDGVWWFIDPDGGRFLSKGVVS
VQFDHDNVQGTDRRPYREACTRKYGSREAWRRVAADRLTSWGFNTLGAWSEPEIARAGTVPLASAAGVVYVATAFGEQRG
GWPLCDIFEPEFEQFARAHARKVCTPQSDDPHVLGWFTDNELPWGPDWRGENELLPTILRAMDAPFSRRAALALLKSRYD
SIDQFNAAWQCKLPSWDDLAATPLQPPPFSRNFFSNDRAQEHDPIARGYFADCDAFAGLLAERYLSVCATAIRAADPNHL
VLGARFAYVPPRDVVDAAGRYCDVISVNCYDPLPDAVIDGYAETGRPCLIGEYSFRGDDAGLPNTHGAGPRMPTQRERAA
GFTRYTTAALRHPALIGYHWFVHADQPAQGRWDGEDSNYGVVTIEDEVYPELTQAMTALNAEAERIHVETAPVLA

Sequences:

>Translated_475_residues
MTGVNVTALRPSEDLRGQQGAAAHGADRHDGMTELARTRWGGLADRKVDASGFFRTEQIDGVWWFIDPDGGRFLSKGVVS
VQFDHDNVQGTDRRPYREACTRKYGSREAWRRVAADRLTSWGFNTLGAWSEPEIARAGTVPLASAAGVVYVATAFGEQRG
GWPLCDIFEPEFEQFARAHARKVCTPQSDDPHVLGWFTDNELPWGPDWRGENELLPTILRAMDAPFSRRAALALLKSRYD
SIDQFNAAWQCKLPSWDDLAATPLQPPPFSRNFFSNDRAQEHDPIARGYFADCDAFAGLLAERYLSVCATAIRAADPNHL
VLGARFAYVPPRDVVDAAGRYCDVISVNCYDPLPDAVIDGYAETGRPCLIGEYSFRGDDAGLPNTHGAGPRMPTQRERAA
GFTRYTTAALRHPALIGYHWFVHADQPAQGRWDGEDSNYGVVTIEDEVYPELTQAMTALNAEAERIHVETAPVLA
>Mature_474_residues
TGVNVTALRPSEDLRGQQGAAAHGADRHDGMTELARTRWGGLADRKVDASGFFRTEQIDGVWWFIDPDGGRFLSKGVVSV
QFDHDNVQGTDRRPYREACTRKYGSREAWRRVAADRLTSWGFNTLGAWSEPEIARAGTVPLASAAGVVYVATAFGEQRGG
WPLCDIFEPEFEQFARAHARKVCTPQSDDPHVLGWFTDNELPWGPDWRGENELLPTILRAMDAPFSRRAALALLKSRYDS
IDQFNAAWQCKLPSWDDLAATPLQPPPFSRNFFSNDRAQEHDPIARGYFADCDAFAGLLAERYLSVCATAIRAADPNHLV
LGARFAYVPPRDVVDAAGRYCDVISVNCYDPLPDAVIDGYAETGRPCLIGEYSFRGDDAGLPNTHGAGPRMPTQRERAAG
FTRYTTAALRHPALIGYHWFVHADQPAQGRWDGEDSNYGVVTIEDEVYPELTQAMTALNAEAERIHVETAPVLA

Specific function: Hydrolyzes agarose to yield predominantly neoagarotetraose and neoagarohexaose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 50 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013529
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02449 Glyco_hydro_42 [H]

EC number: =3.2.1.81 [H]

Molecular weight: Translated: 52593; Mature: 52462

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVNVTALRPSEDLRGQQGAAAHGADRHDGMTELARTRWGGLADRKVDASGFFRTEQID
CCCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCEEEEEEC
GVWWFIDPDGGRFLSKGVVSVQFDHDNVQGTDRRPYREACTRKYGSREAWRRVAADRLTS
EEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
WGFNTLGAWSEPEIARAGTVPLASAAGVVYVATAFGEQRGGWPLCDIFEPEFEQFARAHA
CCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
RKVCTPQSDDPHVLGWFTDNELPWGPDWRGENELLPTILRAMDAPFSRRAALALLKSRYD
HHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SIDQFNAAWQCKLPSWDDLAATPLQPPPFSRNFFSNDRAQEHDPIARGYFADCDAFAGLL
HHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AERYLSVCATAIRAADPNHLVLGARFAYVPPRDVVDAAGRYCDVISVNCYDPLPDAVIDG
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHCCCEEEEEEECCCCCCCHHHHHC
YAETGRPCLIGEYSFRGDDAGLPNTHGAGPRMPTQRERAAGFTRYTTAALRHPALIGYHW
HHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCEEEEEEE
FVHADQPAQGRWDGEDSNYGVVTIEDEVYPELTQAMTALNAEAERIHVETAPVLA
EEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHEEEEECCCCCC
>Mature Secondary Structure 
TGVNVTALRPSEDLRGQQGAAAHGADRHDGMTELARTRWGGLADRKVDASGFFRTEQID
CCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCEEEEEEC
GVWWFIDPDGGRFLSKGVVSVQFDHDNVQGTDRRPYREACTRKYGSREAWRRVAADRLTS
EEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
WGFNTLGAWSEPEIARAGTVPLASAAGVVYVATAFGEQRGGWPLCDIFEPEFEQFARAHA
CCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
RKVCTPQSDDPHVLGWFTDNELPWGPDWRGENELLPTILRAMDAPFSRRAALALLKSRYD
HHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SIDQFNAAWQCKLPSWDDLAATPLQPPPFSRNFFSNDRAQEHDPIARGYFADCDAFAGLL
HHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AERYLSVCATAIRAADPNHLVLGARFAYVPPRDVVDAAGRYCDVISVNCYDPLPDAVIDG
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHCCCEEEEEEECCCCCCCHHHHHC
YAETGRPCLIGEYSFRGDDAGLPNTHGAGPRMPTQRERAAGFTRYTTAALRHPALIGYHW
HHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCEEEEEEE
FVHADQPAQGRWDGEDSNYGVVTIEDEVYPELTQAMTALNAEAERIHVETAPVLA
EEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8193156 [H]