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The map label for this gene is mutB [H]

Identifier: 192290432

GI number: 192290432

Start: 2209283

End: 2211442

Strand: Reverse

Name: mutB [H]

Synonym: Rpal_2039

Alternate gene names: 192290432

Gene position: 2211442-2209283 (Counterclockwise)

Preceding gene: 192290433

Following gene: 192290431

Centisome position: 38.5

GC content: 65.42

Gene sequence:

>2160_bases
ATGAGCAAGATTCCGAACTTCGCCGAGATCGCATTCCAGCCCACTGCCGCCCCTGCCCCTGCAGGTGAGGCCCAGCCGTG
GCTGACGCCCGAAGGCATTCTGGTCAAGCCGGGCTACTCCGAGGCGGATGTCGCCGGCATCGATTTTCTCGACACCTGGC
CGGGCGTCGCGCCGTTCCTGCGCGGCCCCTACCCGACCATGTACGTCAACCAGCCGTGGACGATCCGGCAATATGCCGGC
TTCTCGACCGCTGAAGATTCCAACGCGTTCTATCGCCGCAACCTCGCCGCCGGTCAGAAGGGCCTGTCGGTGGCGTTCGA
CCTCGCCACCCACCGCGGCTACGACTCGGATCATCCGCGCGTCGCCGGCGACGTCGGCATGGCCGGCGTGGCGATCGACT
CGATCTACGACATGCGCACGTTGTTCGCCGGCATCCCGCTCGACCAGATGAGCGTGTCGATGACCATGAACGGCGCGGTG
CTGCCGATCCTGGCGCTGTACGTCGCCGCCGCCGAGGAACAGGGCGTCTCCCCGGAAAAGCTGTCGGGCACCATTCAGAA
CGACATTCTGAAAGAGTTCATGGTGCGCAACACCTACATCTATCCGCCGGTGCCCTCGATGCGGATCATCTCCGACATCT
TCGCCTACACCTCGCAGAAGATGCCGAAGTTCAACTCGATTTCGATCTCCGGCTATCACATGCAGGAAGCCGGCGCGACG
CAGGACCTCGAGCTCGCTTATACGCTCGCCGACGGCGTCGAATACGTCCGCGCCGGCATCGCGGCCGGTCTCGACGTCGA
CAAGTTCGCGCCGCGGCTGTCGTTCTTCTGGGCGATCGGCATGAACTTCTTCATGGAAGTCGCCAAGATGCGCGCCGCGC
GCCTGCTGTGGGCCAAGCTGCTGACGCAGTTCGACCCGAAGCAGCAGAAGTCGCTGAGCCTGCGCACGCACTGCCAGACC
TCCGGTTGGTCGCTGACCGCGCAGGACGTGTTCAACAACGTCTCCCGGACAACTATCGAAGCGATGGCCGCCACCCAGGG
CCACACCCAGTCGCTGCACACCAACGCGCTCGACGAAGCCTTGGCGCTGCCGACGGACTTCTCGGCGCGGATCGCACGCA
ACACCCAGCTGTTCCTGCAGCAGGAGAGCGGCACCACAAGGATCATCGATCCGTGGGGCGGCTCGTACTATGTCGAACGG
CTGACCCACGACATCGCGGTGAAGGCCTGGGCTCACATCCAGGAAGTCGAAGCGCTCGGCGGCATGGCCAAGGCGATCGA
AGCCGGCGTGCCGAAGCTGCGCATCGAGGAAGCTGCCGCCAAGACCCAGGCGCGGATCGACACCGGCCGGCAGTCGGTGA
TCGGCGTCAACAAGTACAAGCCGACCGATGAAACTCCGATCGACGTGCTCAAGGTCGACAATTCGACCGTGCGCCGGCTG
CAGATCGACAAGCTCGGCCGGCTGAAGAAGGAGCGCGTCCAGGCCGATGTTGACGCCGCGTTATTGGCGCTGACCCGCTC
GGCTTCGGAAGGCAACGGCAACCTGTTGGCGCTGGCGATCGATGCGGCGCGCAAGAAAGCCACCGTCGGCGAAATCTCCG
ACGCGCTGGAGAAGGTCTATGGCCGGCATCGCGCCGAGATCAAGTCGATCACCGGCGTCTACAAGCGGGAGGTCGCATCG
ATGGCGGACCGGATGGAGAAGGTGCAGGCGCTGATCGACGCGTTCGAAGACGCCGAGGGCCGTCGCCCGCGTATCCTCGT
CGCCAAGATCGGCCAGGACGGCCACGACCGCGGCCAGAAGGTGATCGCCTCGGCGTTCGCCGACATCGGCTTCGACGTCG
ACATCGGGCCGCTGTTCGCCACCGCCGAGGAGGCGGCGCGGCAGGCGGTGGAGAACGACGTCCACATTCTCGGCGTGTCG
TCGCTGGCCGCCGCGCATCTCACCGCGGTCCCGGAACTCAAGGCCGCGCTGAAGAAGCAGGGTCGCGAGGACATCATGAT
CATCATCGGCGGTGTGGTCCCGCCGCAGGACTACGACGCGCTCTACAAGTCAGGCGCCGAAGCGATCTTCCCGCCCGGCA
CCGTGATCGCCGACGCCGCCGAGGAGTTGATCCACAAGCTGAACGCCCGCCTCGGCCATTCCAGCCAGGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
CACAAGTGTTCAGCTATCAGCAGCCGGACCACGATTAACCCGACGAAATGACGAGAAACGGCAGCCGTGCTACGATGGGC
TGTAGTGAGATCAGATAAAA

Downstream 100 bases:

>100_bases
CTGTCGACTTCGAAAGATGCCGGCTTAATTGACGAACTAGTCTAACCAGACTAGTTTGTCTGCATGAAGCAGGTCGGCAT
TTTCGAAGCAAAGACCCACC

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MSKIPNFAEIAFQPTAAPAPAGEAQPWLTPEGILVKPGYSEADVAGIDFLDTWPGVAPFLRGPYPTMYVNQPWTIRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAV
LPILALYVAAAEEQGVSPEKLSGTIQNDILKEFMVRNTYIYPPVPSMRIISDIFAYTSQKMPKFNSISISGYHMQEAGAT
QDLELAYTLADGVEYVRAGIAAGLDVDKFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLLTQFDPKQQKSLSLRTHCQT
SGWSLTAQDVFNNVSRTTIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVER
LTHDIAVKAWAHIQEVEALGGMAKAIEAGVPKLRIEEAAAKTQARIDTGRQSVIGVNKYKPTDETPIDVLKVDNSTVRRL
QIDKLGRLKKERVQADVDAALLALTRSASEGNGNLLALAIDAARKKATVGEISDALEKVYGRHRAEIKSITGVYKREVAS
MADRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAVENDVHILGVS
SLAAAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSSQAAE

Sequences:

>Translated_719_residues
MSKIPNFAEIAFQPTAAPAPAGEAQPWLTPEGILVKPGYSEADVAGIDFLDTWPGVAPFLRGPYPTMYVNQPWTIRQYAG
FSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAV
LPILALYVAAAEEQGVSPEKLSGTIQNDILKEFMVRNTYIYPPVPSMRIISDIFAYTSQKMPKFNSISISGYHMQEAGAT
QDLELAYTLADGVEYVRAGIAAGLDVDKFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLLTQFDPKQQKSLSLRTHCQT
SGWSLTAQDVFNNVSRTTIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVER
LTHDIAVKAWAHIQEVEALGGMAKAIEAGVPKLRIEEAAAKTQARIDTGRQSVIGVNKYKPTDETPIDVLKVDNSTVRRL
QIDKLGRLKKERVQADVDAALLALTRSASEGNGNLLALAIDAARKKATVGEISDALEKVYGRHRAEIKSITGVYKREVAS
MADRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAVENDVHILGVS
SLAAAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSSQAAE
>Mature_718_residues
SKIPNFAEIAFQPTAAPAPAGEAQPWLTPEGILVKPGYSEADVAGIDFLDTWPGVAPFLRGPYPTMYVNQPWTIRQYAGF
STAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVL
PILALYVAAAEEQGVSPEKLSGTIQNDILKEFMVRNTYIYPPVPSMRIISDIFAYTSQKMPKFNSISISGYHMQEAGATQ
DLELAYTLADGVEYVRAGIAAGLDVDKFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLLTQFDPKQQKSLSLRTHCQTS
GWSLTAQDVFNNVSRTTIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERL
THDIAVKAWAHIQEVEALGGMAKAIEAGVPKLRIEEAAAKTQARIDTGRQSVIGVNKYKPTDETPIDVLKVDNSTVRRLQ
IDKLGRLKKERVQADVDAALLALTRSASEGNGNLLALAIDAARKKATVGEISDALEKVYGRHRAEIKSITGVYKREVASM
ADRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAVENDVHILGVSS
LAAAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSSQAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=714, Percent_Identity=60.2240896358543, Blast_Score=895, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=683, Percent_Identity=61.7862371888726, Blast_Score=843, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=688, Percent_Identity=62.6453488372093, Blast_Score=889, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78119; Mature: 77988

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIPNFAEIAFQPTAAPAPAGEAQPWLTPEGILVKPGYSEADVAGIDFLDTWPGVAPFL
CCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHCCCCHHHHH
RGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
CCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
VAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVLPILALYVAAAEEQGVSPEK
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHHHHHHHHCCCCHHH
LSGTIQNDILKEFMVRNTYIYPPVPSMRIISDIFAYTSQKMPKFNSISISGYHMQEAGAT
HCCHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECEEECCCCCC
QDLELAYTLADGVEYVRAGIAAGLDVDKFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKL
CCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQFDPKQQKSLSLRTHCQTSGWSLTAQDVFNNVSRTTIEAMAATQGHTQSLHTNALDEA
HHHCCCCHHHCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERLTHDIAVKAWAHIQEVEALG
HCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAKAIEAGVPKLRIEEAAAKTQARIDTGRQSVIGVNKYKPTDETPIDVLKVDNSTVRRL
HHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCEEEECCCHHHHHE
QIDKLGRLKKERVQADVDAALLALTRSASEGNGNLLALAIDAARKKATVGEISDALEKVY
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
GRHRAEIKSITGVYKREVASMADRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHH
VIASAFADIGFDVDIGPLFATAEEAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQ
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GREDIMIIIGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSSQAAE
CCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SKIPNFAEIAFQPTAAPAPAGEAQPWLTPEGILVKPGYSEADVAGIDFLDTWPGVAPFL
CCCCCHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHCCCCHHHHH
RGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPR
CCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
VAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVLPILALYVAAAEEQGVSPEK
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHHHHHHHHCCCCHHH
LSGTIQNDILKEFMVRNTYIYPPVPSMRIISDIFAYTSQKMPKFNSISISGYHMQEAGAT
HCCHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECEEECCCCCC
QDLELAYTLADGVEYVRAGIAAGLDVDKFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKL
CCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQFDPKQQKSLSLRTHCQTSGWSLTAQDVFNNVSRTTIEAMAATQGHTQSLHTNALDEA
HHHCCCCHHHCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSYYVERLTHDIAVKAWAHIQEVEALG
HCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAKAIEAGVPKLRIEEAAAKTQARIDTGRQSVIGVNKYKPTDETPIDVLKVDNSTVRRL
HHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCEEEECCCHHHHHE
QIDKLGRLKKERVQADVDAALLALTRSASEGNGNLLALAIDAARKKATVGEISDALEKVY
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
GRHRAEIKSITGVYKREVASMADRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHH
VIASAFADIGFDVDIGPLFATAEEAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQ
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHC
GREDIMIIIGGVVPPQDYDALYKSGAEAIFPPGTVIADAAEELIHKLNARLGHSSQAAE
CCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]